Multi-Omics Analysis of the Mechanism of Mentha Haplocalyx Briq on the Growth and Metabolic Regulation of Fattening Sheep
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Design
2.2. Collection and Determination of Samples
2.3. 16S rRNA Sequencing of Rumen Fluid and Fecal Microorganisms
2.4. Metabolomics Detection of Rumen Fluid, Serum and Urine
2.5. Transcriptomics Analysis
2.6. Statistical Analysis
3. Results
3.1. Growth and Performance
3.2. Characteristics of Intestinal Tissue Morphology
3.3. Analysis of Rumen Fermentation Parameters and Rumen Microbiota
3.4. Analysis of Fecal Microbiota
3.5. Metabolomics Analysis of Rumen Fluid
3.6. Serum Antioxidant Capacity and Metabolomics Analysis
3.7. Urine Metabolomics Analysis
3.8. Analysis of Circular RNA in Rumen Epithelial Tissue
3.9. Analysis of lncRNA in Rumen Epithelial Tissues
3.10. miRNA Analysis of Rumen Epithelial Tissue
4. Discussion
4.1. Growth Performance and Intestinal Tissue Morphology
4.2. Rumen and Fecal Microorganisms
4.3. Metabolomics of Rumen Fluid, Serum, and Urine
4.4. Rumen Epithelial Tissue Transcriptomics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Position | Item | CON (μm) | MHB (μm) |
---|---|---|---|
Duodenum | Muscularis | 431.17 ± 3.29 | 445.01 ± 39.53 |
Villus | 383.11 ± 5.00 | 334.62 ± 29.51 | |
Jejunum | Muscularis | 272.98 ± 5.24 | 219.98 ± 35.05 |
Villus | 362.08 ± 38.72 | 474.07 ± 16.56 | |
Ileum | Muscularis | 161.41 ± 7.32 | 164.53 ± 12.63 |
Villus | 276.27 ± 4.31 | 201.45 ± 9.86 ** | |
Cecum | Muscularis mucosae | 344.66 ± 11.46 | 338.46 ± 13.99 |
Mucosa | 396.35 ± 14.37 | 246.23 ± 8.03 ** | |
Colon | Muscularis mucosae | 276.68 ± 12.00 | 244.32 ± 7.16 |
Mucosa | 535.91 ± 23.65 | 627.06 ± 16.09 * | |
Rectum | Muscularis mucosae | 363.97 ± 8.92 | 418.78 ± 11.66 * |
Mucosa | 351.72 ± 32.90 | 423.86 ± 9.76 |
Item | Treatment | SEM | p-Value | |
---|---|---|---|---|
CON | MHB | |||
Ammonia-nitrogen, mg/dL | 6.21 | 4.00 | 1.1119 | 0.065 |
Total volatile fatty acid, mM | 66.10 | 73.15 | 2.9304 | 0.029 |
Acetate, mM | 45.23 | 51.2 | 2.4436 | 0.027 |
Propionate, mM | 12.21 | 13.4 | 0.9084 | 0.193 |
Butyrate, mM | 7.85 | 7.72 | 0.5670 | 0.820 |
Valerate, mM | 0.81 | 0.78 | 0.0456 | 0.592 |
Acetate: Propionate | 3.71 | 3.94 | 0.3498 | 0.530 |
Phylum | Genus | Treatment | SEM | p-Value | |
---|---|---|---|---|---|
CON | MHB | ||||
Bacteroidota | Paraprevotella | 0.708 | 1.333 | 0.282 | 0.043 |
Alloprevotella | 0.028 | 0.097 | 0.028 | 0.029 | |
Marinilabilia | 0.013 | 0.070 | 0.026 | 0.044 | |
Candidatus Saccharibacteria | Saccharibacteria_genera_incertae_sedis | 0.110 | 0.283 | 0.072 | 0.030 |
Bacillota | Blautia | 0.150 | 0.083 | 0.031 | 0.048 |
Verrucomicrobia | Subdivision5_genera_incertae_sedis | 0.065 | 0.113 | 0.019 | 0.022 |
Chloroflexota | Ornatilinea | 0.118 | 0.025 | 0.021 | <0.01 |
Phylum | Genus | Treatment | SEM | p-Value | |
---|---|---|---|---|---|
CON | MHB | ||||
Bacteroidota | Prevotella | 1.662 | 3.960 | 0.998 | 0.036 |
Bacillota | Blautia | 0.947 | 0.482 | 0.213 | 0.046 |
Coprococcus | 0.700 | 0.412 | 0.118 | 0.028 | |
Clostridium XlVb | 0.203 | 0.320 | 0.050 | 0.035 | |
Parasutterella | 0.148 | 0.068 | 0.024 | 0.005 |
Item | Treatment | SEM | p-Value | |
---|---|---|---|---|
CON | MHB | |||
GSH-Px | 287.419 | 351.290 | 22.455 | 0.012 |
MDA | 2.970 | 2.094 | 0.598 | 0.164 |
SOD | 15.551 | 20.061 | 0.639 | <0.01 |
T-AOC | 0.288 | 0.555 | 0.080 | 0.004 |
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Yi, M.; Cao, Z.; Zhou, J.; Ling, Y.; Zhang, Z.; Cao, H. Multi-Omics Analysis of the Mechanism of Mentha Haplocalyx Briq on the Growth and Metabolic Regulation of Fattening Sheep. Animals 2023, 13, 3461. https://doi.org/10.3390/ani13223461
Yi M, Cao Z, Zhou J, Ling Y, Zhang Z, Cao H. Multi-Omics Analysis of the Mechanism of Mentha Haplocalyx Briq on the Growth and Metabolic Regulation of Fattening Sheep. Animals. 2023; 13(22):3461. https://doi.org/10.3390/ani13223461
Chicago/Turabian StyleYi, Mingliang, Zhikun Cao, Jialu Zhou, Yinghui Ling, Zijun Zhang, and Hongguo Cao. 2023. "Multi-Omics Analysis of the Mechanism of Mentha Haplocalyx Briq on the Growth and Metabolic Regulation of Fattening Sheep" Animals 13, no. 22: 3461. https://doi.org/10.3390/ani13223461