Copyright
by
Jennifer Alane Tate
2002
The Dissertation Committee for Jennifer Alane Tate certifies that this is the
approved version of the following dissertation:
Systematics and Evolution of Tarasa Philippi (Malvaceae):
An enigmatic Andean polyploid genus
Committee:
Beryl B. Simpson, Supervisor
David M. Hillis
Robert K. Jansen
Donald A. Levin
José L. Panero
Systematics and Evolution of Tarasa Philippi (Malvaceae):
An enigmatic Andean polyploid genus
by
Jennifer Alane Tate, B.S.
Dissertation
Presented to the Faculty of the Graduate School of
The University of Texas at Austin
in Partial Fulfillment
of the Requirements
for the Degree of
Doctor of Philosophy
The University of Texas at Austin
August, 2002
Acknowledgements
This dissertation certainly would not have come to fruition without the
assistance of many individuals.
I would first like to thank my advisor, Beryl
Simpson, for her continual support and guidance through the years. My committee
members: Bob Jansen, Don Levin, José Panero, and David Hillis were each helpful at
various stages of my graduate career. I would also like to thank Alan Lloyd for
serving on my qualifying exam.
The botany graduate students at UT were a major factor in my decision to join
the program.
My cohorts in the Simpson lab have provided an unparalleled
stimulating environment to work in over the years. Megan Helfgott was unbelievably
patient with me as a newcomer to the lab. She was ever encouraging and never
laughed at my ignorance in molecular ways. Martín Timaná, my Andean brother,
translated many Spanish documents for me and was a wonderful travel companion
during two trips to South America. Heidi Meudt and Mauricio López Langenbach
were likewise a great pair to travel with in Chile. Heidi’s enthusiasm for the natural
world is infectious. Mauricio (an honorary member of the Simpson lab) translated
Spanish and German documents and introduced me to the wonderful world of
empanadas! Fellow GOBOGS gals Katie Hansen, Andrea Weeks, and Leah Larkin
were always up for stimulating conversations over beer and offered many helpful
suggestions. Sarah Simmons knows me better than most and is still my friend.
Vaughan Symonds provided daily personal and professional support for which I am
most grateful. Many other graduate students were a welcome source of knowledge
and humor through the years, particularly Todd Barkman, Karen Clary, Jennifer
iv
Clevinger, Geoff Denny, Doug Goldman, Joshua McDill, Gani Padolina, Anneke
Schulmann, Ruth Timme, and Brian Van den Heuvel.
I have benefited from the wisdom and generosity of many systematists
throughout my degree program. Mark Chase (Kew Gardens) sent DNA aliquot of
Tarasa humilis.
John La Duke (University of North Dakota) sent DNA, ITS
sequences for the Malveae chapter, and lended advice for germinating mallow seeds.
Javier Fuertes Aguilar (Real Jardin Botanico, Madrid) and Tracey Slotta (Virginia
Polytechnic Institute) provided sequences for the Malveae chapter. Javier taught me
to “just add water”. Derrick Zwickl provided much needed assistance with using the
program Seq-gen and the use of his program Logreader. Zaiming Zhao taught me the
invaluable skill of identifying pollen mother cells in chromosome squashes. Jonathan
Wendel offered sequencing advice early in my study. Paul Fryxell provided his
amazing Malvaceae specimen and literature collection, as well as his superior
knowledge of the family many times.
Many staff members at UT were instrumental in various ways during the
dissertation process. I would particularly like to thank Theresa Barnes, Susan Cook,
John Lucio, Sandy Monahan, Tamra Rogers, Dena Sutton, and Paul Ward. John
Mendenhall took the SEM photos in Chapter 4. I would also like to thank the
curators of the herbaria from which I borrowed specimens (AAU, B, BM, C, CAS,
CONC, CORD, CTES, F, G, GB, GH, GOET, K, LPB, MEXU, MO, NY, OS, S,
SGO, SI, TEX/LL, UC, UPS, US, USM), particularly those that allowed me to
remove material for various studies. A special thanks is deserved to Tom Wendt for
all his efforts.
v
I am particularly thankful that my family has always been encouraging of my
desire to pursue a botanical career. Their support of my nerdy endeavors has not
gone unnoticed.
Funding for this project was provided by the National Science Foundation,
Doctoral Dissertation Improvement Grant; Botanical Society of America, J.S. Karling
Award; American Society of Plant Taxonomists, Graduate Student Research Award;
Sigma Xi, Grants-in-Aid of Research; University of Texas at Austin, Institute of Latin
American Studies, Faculty Sponsored Grant for Field Research in Latin America;
University of Texas at Austin, Department of Botany, Graduate Student Research
Award. I am also thankful for a University Continuing Fellowship that allowed me to
make great progress on my dissertation.
vi
Systematics and Evolution of Tarasa Philippi (Malvaceae):
An enigmatic Andean polyploid genus
Publication No._____________
Jennifer Alane Tate, Ph.D.
The University of Texas at Austin, 2002
Supervisor: Beryl B. Simpson
This work represents a progression from a large scale molecular phylogeny to
a species level study of breeding systems.
A phylogenetic approach utilizing
molecular sequence data was employed to evaluate the current infratribal
circumscription and generic relationships within the tribe Malveae (subfamily
Malvoideae, Malvaceae). The resulting ITS phylogeny indicates that many of the
alliances as well as several genera are not monophyletic and suggests that the current
classification needs revision. An in depth phylogenetic examination of one Malveae
genus, Tarasa Philippi, and suggested close relatives, Nototriche Turcz. and
Sphaeralcea St.-Hil, was also conducted using nuclear and chloroplast sequence data.
The monophyly of Tarasa was rejected in both the nuclear and chloroplast trees.
Surprisingly, the morphologically similar tetraploid species also did not form a single
clade, which indicates that the tetraploids have been formed multiple times.
Geographic and molecular data suggest that the tetraploid species may have been
vii
derived from multiple encounters between different sympatric diploid annual species.
Although multiple origins of polyploidy in Tarasa is not a novel finding, the apparent
morphological convergence of the tetraploid species is most intriguing particularly
because they violate two traditional dogmas of polyploids: they are annuals and have
smaller pollen grains than their diploid relatives. A study of the breeding systems and
pollen characteristics for Tarasa diploid and tetraploid species also revealed
unexpected findings. Pollen/ovule (p/o) ratios were used as an indirect measure of
the degree of outcrossing versus selfing and greenhouse studies provided direct
evidence of self-compatibility as well as autogamy in the plants. Tarasa species
follow the predicted trend of low p/o ratios in autogamous species and high p/o ratios
in xenogamous species. However, the overall positive correlation observed in Tarasa
between p/o ratio and pollen size contradicts the expected negative relationship
between these two factors. Statistically significant differences were detected between
the tetraploids and diploid annuals and perennials for anther number per flower,
number of grains per anther, p/o ratio, and pollen size. The tetraploid species occupy
the highest elevation habitats in the Andes and have repeatedly adopted a floral
morphology which is typical of autogamous species.
viii
Table of Contents
List of Tables ........................................................................................................ xii
List of Figures .................................................................................................... xviii
Chapter 1: An introduction to the dissertation........................................................1
Chapter 2: Phylogenetic relationships within the Tribe Malveae (Malvoideae,
Malvaceae) as inferred from ITS sequence data.............................................4
Abstract ...........................................................................................................4
Introduction.....................................................................................................4
Materials and Methods..................................................................................11
Taxon sampling....................................................................................11
DNA extraction and amplification.......................................................12
Sequence analysis ................................................................................18
Phylogenetic analysis...........................................................................18
Results...........................................................................................................19
Sequence characteristics and phylogenetic reconstruction ..................19
Discussion .....................................................................................................25
The traditional alliances (as well as several genera) are not
monophyletic...............................................................................25
Alliances and genera of Clade A .........................................................27
Alliances and genera of Clade B..........................................................29
Conclusions...................................................................................................32
Chapter 3: Molecular systematics of the polyploid Andean genus Tarasa Philippi
(Malvaceae)...................................................................................................33
Abstract .........................................................................................................33
Introduction...................................................................................................34
Taxonomic history ...............................................................................34
Chromosomes, geography, and morphology .......................................35
Systematic position ..............................................................................37
Materials and Methods..................................................................................40
ix
Taxon sampling....................................................................................40
DNA extraction and amplification.......................................................41
Sequence analysis ................................................................................47
Phylogenetic reconstruction.................................................................48
Chromosome counts.............................................................................50
Results...........................................................................................................52
New chromosome counts.....................................................................52
Chloroplast sequence characteristics ...................................................52
Chloroplast phylogeny reconstruction .................................................55
ITS sequence characteristics ................................................................60
ITS phylogeny reconstruction..............................................................62
Discussion .....................................................................................................65
Taxon sampling....................................................................................65
Urocarpidium albiflorum is not a Tarasa............................................65
Chloroplast phylogeny .........................................................................66
ITS phylogeny......................................................................................70
Hypothesis testing and incongruent phylogenies.................................75
Recovering the organismal phylogeny: Gene trees versus species trees76
Chapter 4: Multiple origins of polyploidy and breeding system evolution in
Tarasa (Malvaceae) ......................................................................................78
Abstract .........................................................................................................78
Introduction...................................................................................................79
Materials and Methods..................................................................................81
Pollen/Ovule ratios...............................................................................81
Self-compatibility in greenhouse grown plants ...................................84
Pollen characteristics ...........................................................................85
Results...........................................................................................................86
Pollen/ovule ratios ...............................................................................86
Self seed set..........................................................................................93
Pollen characteristics ...........................................................................94
Discussion ...................................................................................................100
x
A shift in breeding system follows polyploidization in Tarasa.........100
Tarasa tetraploid pollen size contradicts breeding system and ploidy
level expectations......................................................................103
Appendix A. Malveae ITS alignment ................................................................105
Appendix B. Tarasa and outgroups combined chloroplast data matrix ............126
Appendix C. Tarasa and outgroups ITS alignment ...........................................162
Literature cited .....................................................................................................172
Vita .....................................................................................................................183
xi
List of Tables
Table 2.1.
Genera of the tribe Malveae (Malvoideae, Malvaceae), their alliance
associations, haploid chromosome numbers and geographic
distributions. Abbreviations for alliances are as follows: A =
Abutilon, AN = Anoda, AS = Anisodontea, BK = Bakeridesia, BT =
Batesimalva, F = Fryxellia, G = Gaya, H = Howittia, HE =
Herissantia, K = Kearnemalvastrum, M = Malva, MA = Malope,
MO=Modiola, MS = Malvastrum, N = Napaea, PG = Plagianthus,
PH = Phymosia, R = Robinsonella, SC = Sidalcea, SD = Sida, SP =
Sphaeralcea.........................................................................................6
Table 2.2.
Historical classification of genera currently placed in tribe Malveae
(subfamily Malvoideae, Malvaceae)...................................................9
Table 2.3.
List of included taxa, voucher information (or GenBank accession
number), and collection locality information. *Where two accession
numbers occur, the first corresponds to the ITS1 sequence and the
second to ITS2. JL indicates DNA provided by John La Duke
(University of North Dakota), JFA was provided by Javier Fuertes
Aguilar (Real Jardin Botanico, Madrid), and TBS indicates DNA
provided by Tracey Bodo Slotta. ......................................................13
Table 2.4
Analysis of ITS regions for taxa included in this study. Length and
GC content were calculated using MacClade v. 4.0 (Maddison and
Maddison 2000). Pairwise divergence values and number of
parsimony informative characters were found using PAUP* v.4.0b8
(Swofford 2002)................................................................................20
xii
Table 2.5
Test for base composition bias of analyzed sequences (for entire data
set including outgroups). Values given are mean base frequency,
degrees of freedom (df), and P value (a value greater than 0.05
indicates that there is not a significant difference among the taxa in
the data set). ......................................................................................20
Table 3.1
Tarasa as circumscribed (Krapovickas 1954, 1960, 1971; Bates
1965) and chromosome numbers. Ploidy level was inferred for
species without previously published chromosome counts – see page
50 for details. ....................................................................................36
Table 3.2
Vouchers used in molecular analysis, their location and GenBank
accession number. An asterisk indicates voucher for chromosome
count. A letter designates separate populations sampled.................42
Table 3.3
List of primers used for PCR amplification and sequencing ............45
Table 3.4
Sequence statistics for species of Tarasa as calculated in MacClade
(length and GC content) and PAUP* (pairwise divergence). ...........53
Table 3.5
Test for base composition bias of analyzed sequences (for entire data
set including outgroups). Values given are mean base frequency,
degrees of freedom (df), and P value (a value greater than 0.05
indicates that there is not a significant difference among the taxa in
the data set). ......................................................................................54
xiii
Table 3.6
Results from the Independent Length Difference (ILD) Test (Farris
1994) for the molecular regions sequenced (ITS, matK-3’trnK
intron, psbA-trnH spacer, and trnT-trnL spacer). Data partitions
were defined in PAUP* and tested for homogeneity. The null
hypothesis (that the data partitions are homogenous) was rejected at
the 0.05 level.....................................................................................54
Table 3.7
Results from constraint analyses using parametric bootstrapping on
the ITS and chloroplast data sets. The first constraint forced all
traditionally circumscribed Tarasa to monophyly; the second
constraint was as the first but excluded the n=6 group of Tarasa
machupicchensis, T. mandonii, and T. spiciformis; the third
constraint forced monophyly of all tetraploids (chromosome number
known and inferred based on morphology and geography – see text
for details). The empirical tree length differences (=constrainedunconstrained) were compared to a distribution of tree length
differences generated from 100 simulated data sets. The null
hypothesis (that the constraint tree is the true tree) was rejected when
the observed tree length difference was greater than 95% of the
simulated tree length differences. *For the chloroplast unconstrained
tree, gaps were coded as missing. .....................................................59
Table 3.8
Results of three incongruence tests as conducted in PAUP*. All
tests compared the strict consensus of the most parsimonious ITS
trees and the strict consensus of the most parsimonious chloroplast
trees. The null hypothesis that the data fit either tree equally well
was rejected at the 0.05 level. ...........................................................60
xiv
Table 3.9
Hypothesized relationships for tetraploid species of Tarasa
(chromosomally known and inferred) based on nuclear (paternal
parent) and chloroplast (maternal) phylogeny reconstructions. An
asterisk indicates a tetraploid for which the chromosome number was
inferred. Hypotheses are based on the most closely related diploid
species to the tetraploid in each phylogeny. .....................................69
Table 3.10 Areas of sympatry between diploid species of Tarasa that are
potential tetraploid parents (see Table 3.9). Perennial species are
indicated by an asterisk. n/a indicates no apparent areas of overlap
in geographic distribution based on mapping of 1400 herbarium
specimen localities. ...........................................................................71
Table 4.1
Typical attributes of polyploid taxa as compared to Tarasa
polyploids..........................................................................................79
Table 4.2
Vouchers used in pollen/ovule ratio analysis....................................83
Table 4.3.
Results of pollen/ovule ratio analysis for species of Tarasa. An
asterisk indicates inferred chromosome number on the basis of
morphology and geographic distribution (see text for explanation).
Means and standard deviations (in parentheses) are given for number
of grains per anther, number of anthers per flower, and number of
ovules per flower. Breeding system was determined by comparing
the empirical log p/o ratios to values reported by Cruden (1977) (X =
xenogamy, FX = facultative xenogamy, FA = facultative autogamy,
and OA = obligate autogamy) – see text for details..........................88
xv
Table 4.4
Means and standard deviations (in parentheses) of anther number per
flower, number of grains per anther, and pollen/ovule ratio for ploidy
level and habit categories in Tarasa (see Table 4.3 for details).
Significance levels as detected by ANOVA are also given for these
categories. .........................................................................................92
Table 4.5
P-values for mean anther number per flower, number of grains per
anther, and P/O ratio as determined by Fisher’s PLSD and
Bonferroni/Dunn post hoc ANOVA for diploid (2n=10 only shown)
and tetraploid (2n=20) species of Tarasa. Calculated P values were
the same for each test. Significant differences are indicated as
follows: * indicates a p-value significant under Fisher’s PLSD at the
0.05 level, ** indicates a p-value significant under Bonferroni/Dunn
at the 0.05 level, *** indicates significant difference under both tests
(0.05 level). .......................................................................................93
Table 4.6
Self seed set in greenhouse grown individuals of Tarasa. Plants
were grown in the greenhouse at The University of Texas at Austin.
The number of fully mature seeds per schizocarp was counted for
fifty schizocarps on a single plant. Autogamous seed set is the
proportion of mature seeds produced to the number of carpels
present. Standard deviations are given in parentheses. An asterisk
indicates an inferred chromosome number on the basis of
morphology and geographic distribution (see text for details). ........94
xvi
Table 4.7
Results of pollen measurements utilizing light microscopy. Twentyfive grains were measured per individual; when possible 2 or more
individuals were sampled to represent a species. An asterisk
inidicates an inferred chromosome number based on morphology
and geographic distribution (see text for details). Values given are
the mean and standard deviation for the polar and equatorial axis,
and the approximate number of apertures.........................................95
Table 4.8
Means and standard deviations for pollen grain size (polar and
equatorial diameters) for species of Tarasa studied. P values from
ANOVA are given for the categories of ploidy level and ploidy level
and habit for all taxa (chromosome number inferred) or
chromosomally known taxa only. .....................................................97
Table 4.9
Significance levels for mean pollen diameter (polar axis) as
determined by Fisher’s PLSD and Bonferroni/Dunn post hoc
ANOVA for diploid (2n=10 only shown) and tetraploid (2n=20)
species of Tarasa. Calculated P values were the same for each test.
Significant differences are indicated as follows: * indicates a pvalue significant under Fisher’s PLSD at the 0.05 level, ** indicates
a p-value significant under Bonferroni/Dunn at the 0.05 level, ***
indicates significant difference under both tests (0.05 level). ..........99
xvii
List of Figures
Figure 2.1
One of 53 most parsimonious trees based on ITS sequence data
[length=2992, CI= 0.2920 (excluding autapomorphies), RI= 0.6562,
and RC=0.2112]. Branch lengths are shown above the branches.
Alliance groupings are shown at right. .............................................21
Figure 2.2
The strict consensus of 53 most parsimonious trees based on ITS
sequence data. Bootstrap support (above 50) for 100 replicates is
indicated below the nodes.................................................................22
Figure 2.3
Clade A from one most parsimonious tree (see Figure 2.1). Branch
lengths are shown above the branches and bootstrap support (above
50) for 100 replicates is given below. Genera are colored according
to their alliance associations which also correspond to the bars on the
right. ..................................................................................................23
Figure 2.4
Clade B from one most parsimonious tree (see Figure 2.1). Branch
lengths are shown above the branches and bootstrap support (above
50) for 100 replicates is given below. Genera are colored according
to their alliance associations which also correspond to the bars on the
right. ..................................................................................................24
Figure 3.1
Distribution of Tarasa in the Americas. The map was generated
using the program DIVA-GIS (Hijmans et. al 2002). Localities are
based on label information from approximately 1400 herbarium
specimens. Diploid (2n=10) species are indicated by red dots;
tetraploid (2n=20) species are indicated by yellow dots...................38
xviii
Figure 3.2
One of 15 most parsimonious trees based on chloroplast sequence
data (length=619, CI=0.713, RI=0.873, RC=0.735). Branch lengths
are given above the branches. Dashed branches collapse in the strict
consensus. Diploid (2n=10) perennial species of Tarasa are shown
in blue, diploid annual species are shown in red, and tetraploid
annuals are shown in green. The 2n=12 group is shown in orange.
An asterisk following a taxon name indicates inferred ploidy level
(see page 50 for details). Letters following taxon names indicate a
separate population sampled. ............................................................57
Figure 3.3
Strict consensus of 15 most parsimonious trees based on chloroplast
sequence data. Bootstrap support (above 50) for 100 replicates is
shown below the branches. Diploid (2n=10) perennial species of
Tarasa are shown in blue, diploid annual species are shown in red,
and tetraploid annuals are shown in green. The 2n=12 group is
shown in orange. An asterisk following a taxon name indicates
inferred ploidy level (see page 50 for details). Letters following
taxon names indicate a separate population sampled. ......................58
xix
Figure 3.4
One of 7 most parsimonious trees based on ITS sequence data
(length=348, CI=0.6084, RI=0.8360, RC=0.5677). Branch lengths
are shown above the branches. Dashed branches collapse in the
strict consensus. Diploid (2n=10) perennial species of Tarasa are
shown in blue, diploid annual species are shown in red, and
tetraploid annuals are shown in green. The 2n=12 group is shown in
orange. An asterisk following a taxon name indicates inferred ploidy
level (see page 50 for details). Letters following taxon names
indicate a separate population sampled; a number following the
name indicates clone number............................................................63
Figure 3.5
Strict consensus of 7 most parsimonious trees based on ITS sequence
data. Bootstrap support (above 50) for 100 replicates is shown
below the branches. Diploid (2n=10) perennial species of Tarasa
are shown in blue, diploid annual species are shown in red, and
tetraploid annuals are shown in green. The 2n=12 group is shown in
orange. An asterisk following a taxon name indicates inferred ploidy
level (see page 50 for details). Letters following taxon names
indicate a separate population sampled; a number following the
name indicates clone number............................................................64
Figure 4.1
Histogram of mean number of anthers per flower for all species of
Tarasa analyzed. ...............................................................................90
Figure 4.2
Histogram depicting mean number of grains per anther for all
species of Tarasa analyzed. ..............................................................91
Figure 4.3
Histogram of mean pollen/ovule ratio for all species of Tarasa
analyzed. ...........................................................................................91
xx
Figure 4.4
Scanning electron micrographs of pollen grains for 6 species of
Tarasa (Malvaceae) demonstrating variation in grain size and spine
size and morphology. Magnification and scale bars are given in the
lower right corner of each panel. Panels A-C: Tetraploid annual
species, panels D-E: Diploid perennials, panel F: Diploid annual. ..96
Figure 4.5
Histogram of mean pollen diameter (polar axis) in micrometers for
the categories of diploid annual, diploid perennial, and tetraploid
annual................................................................................................99
Figure 4.6
Relationship between surface area of pollen grain (polar axis) and
pollen/ovule ratio for species of Tarasa studied (see Table 4.3).
Tetraploid annual species are shown in red, diploid annuals in light
blue, and diploid perennials in dark blue. Pearson correlation
coefficients (r) for the tetraploids, diploid annuals, and diploid
perennials are 0.519, -0.698, and 0.296, respectively.....................100
xxi
Chapter 1: An introduction to the dissertation
The purpose of this chapter is to serve as an overall introduction to the
dissertation. This work represents a progression from a large scale molecular phylogeny
to a species level study of breeding systems. As Chapters 2-4 are intended ultimately to
be separate publications, they are constructed as such with an abstract, introduction,
materials and methods, results, and discussion with a single literature cited compiled for
all chapters.
Chapter 2 involves a molecular phylogenetic analysis of the tribe Malveae
(subfamily Malvoideae, Malvaceae) using sequence data from the nuclear ribosomal
internal transcribed spacers (ITS).
The phylogeny was reconstructed using newly
generated sequences, sequences deposited in GenBank, and sequences generously
provided by other Malvaceae workers (Javier Fuertes Aguilar, Tracey Bodo Slotta, and
John La Duke). The tribe Malveae is the largest in the subfamily Malvoideae, containing
approximately 75 genera (~1000 species). Various circumscriptions of the tribe have
been published over the past 150 years. Since 1968, however, the genera within it have
been assigned to twenty-one informal alliances on the basis of morphology and
chromosome number. The ITS phylogeny indicates that many alliances and also several
genera are not monophyletic. The basalmost lineage of the Malveae is occupied by the
genus Howittia which is sister to two larger clades containing the remaining members of
the tribe. According to the ITS phylogeny, chromosomal evolution appears to have been
quite complex within the tribe as aneuploid and polyploid lineages commonly occur.
Chapter 3 investigates in detail the systematics and evolution of one of the
Malveae genera (Tarasa) and its relatives. Tarasa Philippi is a genus found in the high
1
Andes from central Peru to southern Chile and adjacent Argentina with two species
disjunct in Mexico.
The species exhibit a distinct geographic distribution that is
concordant with ploidy level: the diploids are found in pockets around the Andes at midelevations (800-3000 m), while the tetraploids are widespread at the highest elevations
(3000-4200 m). The tetraploid species of Tarasa are unusual in that they are annuals and
have smaller morphological features than the diploids. Molecular phylogenies were
reconstructed for most species of the genus and several purportedly allied genera using
nuclear and chloroplast sequence data. The monophyly of Tarasa was rejected in both
the nuclear and chloroplast phylogenies. Also surprising was the result that the tetraploid
species did not form a monophyletic clade, which indicates that polyploidy has arisen
multiple times within the genus. While the independent origin of polyploids within a
genus is not unique to Tarasa, the apparent morphological convergence of the tetraploids
subsequent to polyploidy is intriguing. Based on the sequence data, it appears that
various combinations of the diploid annual species of Tarasa have formed these unusual
tetraploids.
The discoveries from Chapter 3 prompted a study of the breeding systems of
Tarasa species, which is presented in Chapter 4. Pollen/ovule ratios have often been
used as an indicator of the relative degree of outcrossing versus selfing in plants. For
many plant taxa, a negative correlation has been found between pollen/ovule ratios and
the degree of self-fertilization, pollen grain size, and stigmatic area relative to the pollen
bearing area of the pollinator (Cruden 1977, 2000). Species of Tarasa follow this trend
for breeding systems: the diploid perennials have the highest p/o ratios (xenogamous),
followed by the diploid annuals (xenogamous to facultatively xenogamous), and the
tetraploid annuals have the lowest p/o ratios (autogamous or facultatively xenogamous).
All pairwise comparisons were found to be statistically significant.
2
Empirical data
indicate that the tetraploids not only have fewer anthers per flower, but also have fewer
pollen grains produced per anther. In the greenhouse, none of the diploid perennials (5
species studied) were self-compatible. Only one diploid annual species was available for
study and it was autogamous, but the resulting progeny were weak (likely a result of
inbreeding depression). All of the six tetraploid species studied were autogamous and
produced vigorous offspring. Thus, in Tarasa the multiple origins of polyploidy have
been accompanied by numerous shifts to an autogamous breeding system. An intriguing
earlier finding suggested that Tarasa tetraploids have smaller pollen grains than the
diploids (Tressens 1970). Because polyploidization is expected to increase cell size,
polyploids typically have larger pollen and guard cells than their diploid relatives
(Stebbins 1940). A thorough reexamination of pollen grain sizes for 27 of the 30 species
using light microscopy confirmed that the tetraploids do in fact have smaller grains than
the diploids. The difference in size is statistically significant between the tetraploids and
diploid annuals and perennials. These data are also surprising since, unlike most plant
groups, a positive correlation was found between pollen grain size and p/o ratio for the
tetraploids and diploid perennials (although the diploid annuals exhibited a negative
correlation). The unusual and recurring morphology exhibited by the tetraploids is quite
intriguing (annual habit, inconspicuous white flowers with few anthers).
Potential
contributing factors during the evolution of these species are morphological adaptations
to a high elevation habitat, a reduction in pollinator abundance at such altitudes, and
adaptations that accompany a self-compatible breeding system.
3
Chapter 2: Phylogenetic relationships within the Tribe Malveae
(Malvoideae, Malvaceae) as inferred from ITS sequence data
ABSTRACT
A molecular phylogenetic analysis of the tribe Malveae (subfamily Malvoideae,
Malvaceae) was undertaken using the internal transcribed spacer region of the 18S-26S
nuclear ribosomal repeat (ITS).
This work represents the first exhaustive study to
evaluate the infratribal circumscription and generic affinities using molecular sequence
data. The ITS phylogeny indicates that the informal tribal alliances and several genera
are not monophyletic. Two main clades were reconstructed in the phylogeny: one clade
pertaining to the Abutilon, Bakeridesia, Batesimalva, Fryxellia, Gaya, Plagianthus,
Robinsonella, and Sida alliances, and a second large clade containing genera from the
Anisodontea, Callirhoe, Kearnemalvastrum, Malva, Malvastrum, Modiola, Napaea,
Phymosia, Sphaeralcea, and Sidalcea alliances.
INTRODUCTION
In recent years, morphological and molecular evidence have shown that many of
the traditional families of the Malvales are not monophyletic (Judd and Manchester 1997;
Alverson et al. 1998, 1999; Bayer et al. 1999). As a result, an expanded Malvaceae has
been proposed which is comprised of the subfamilies Bombacoideae (formerly
Bombacaceae), Sterculioideae (Sterculiaceae), Malvoideae (Malvaceae), and Tiliodeae
(Tiliaceae) (Bayer et al. 1999). Subfamily Malvoideae has consistently been supported as
a monophyletic group on the basis of both morphological and molecular data (Judd and
Manchester 1997, Alverson et al. 1999, Bayer et al. 1999). Although no classification for
the Malvoideae has yet been offered, I will here refer to this group as most recently
4
circumscribed:
composed of the tribes Dechaschistieae, Gossypieae, Hibisceae,
Malvavisceae, and Malveae (Fryxell 1988).
As considered in the present study, the tribe Malveae includes approximately 75
genera (~1000 species) which encompass the majority of the morphological and
taxonomic diversity within the family (Table 2.1) (Fryxell 1997). Traditionally, members
of the Malveae have been characterized by a combination of several morphological
characters: schizocarpic fruits (sometimes a capsule), mericarps numbering 3 to over 20
and equal to the number of free styles, antheriferous apex of the staminal column, and the
absence of lysigenous cavities (“gossypol glands”) (Fryxell 1988). The genera of the
Malveae exhibit a broader geographic distribution and exploit a wider variety of habitats
than the other malvaceous tribes. While most of the other tribes are restricted to tropical
and subtropical zones of both hemispheres, more than 15 of the 75 Malveae genera have
mostly temperate distributions.
However, some of the largest genera of the tribe
(Abutilon, Sida, Nototriche) have tropical distributions (Table 2.1).
The genera currently considered members of the tribe Malveae have not always
been placed in this tribe.
Table 2.2 provides a synopsis of the major infratribal
classification schemes, beginning with Bentham and Hooker (1862), for the tribe
Malveae and for the genera currently considered members of the Malveae. Bentham and
Hooker divided the tribe into four subtribes on the basis of carpel arrangement and ovule
number and position:
Abutilinae, Malopinae, Malvinae, and Sidinae (as Abutileae,
Eumalveae, Malopeae, and Sideae). Schumann (1890) reassigned three genera (Malope,
Kitaibelia, Palaua) to a separate tribe Malopeae due to the irregular arrangement of their
carpels into superimposed verticils (i.e., not in single whorl). The remaining genera of
the Malveae were placed into one of three subtribes (Abutilinae, Malvinae, or Sidinae
[Schumann 1890]) based on carpel morphology. This classification was followed by
5
Table 2.1.
Genera of the tribe Malveae (Malvoideae, Malvaceae), their alliance
associations, haploid chromosome numbers and geographic distributions.
Abbreviations for alliances are as follows: A = Abutilon, AN = Anoda, AS
= Anisodontea, BK = Bakeridesia, BT = Batesimalva, F = Fryxellia, G =
Gaya, H = Howittia, HE = Herissantia, K = Kearnemalvastrum, M = Malva,
MA = Malope, MO=Modiola, MS = Malvastrum, N = Napaea, PG =
Plagianthus, PH = Phymosia, R = Robinsonella, SC = Sidalcea, SD = Sida,
SP = Sphaeralcea.
GENUS
ALLIANCE
HAPLOID
DISTRIBUTION
NUMBER
Abutilon Mill.
A
Acaulimalva Krapov.
Akrosida Fryxell & Fuertes
Alcea L.
Allosidastrum (Hochr.)
Krapov., Fryxell & D.M.
Bates
Allowissadula D.M. Bates
Althaea L.
Anisodontea Presl
Anoda Cav.
SP
SD
M
SD
A
M
AS
AN
7, 8, 14, 16, 18,
21, 36
5
NA
21
7
Pantropical
Andes
Brazil
Mediterranean to C Asia
Neotropics
Asterotrichion Klotzsch
Bakeridesia Hochr.
Bastardia H.B.K.
Bastardiastrum (Rose) D.M.
Bates
Bastardiopsis (K. Schum.)
Hassl.
Batesimalva Fryxell
Billieturnera Fryxell
Briquetia Hochr.
Callirhoe Nutt.
PG
BK
A
A
8
14, 21, 25, 35, 42
22
13, 14, 15, 18,
30, 45
NA
15
7, 14
15
A
14
S Am
BT
A
BT
SC
Calyculogygas Krapov.
Calyptraemalva Krapov.
Corynabutilon (K. Schum.)
Kearney
Cristaria Cav.
Dendrosida Fryxell
Dirhamphis Krapov.
SP
SP
A
12, 16
8
7
14, 15, 21, 28,
30, 34, 35, 42, 56
5
NA
8
Mexico
S Texas to NE Mexico
Mexico, S Am
C United States to NE
Mexico
Uruguay
Brazil
Temperate Chile & Argentina
G
SD
BT
6, 12
21
7, 15
Chile, also S Peru
S Mexico, N South America
W Mexico, Bolivia/Paraguay
6
Texas, Mexico
Europe, Middle East, C Asia
S Africa
S US, Mexico, S Am
Tasmania
Mexico, C Am
Neotropics
W Mexico
Eremalche Greene
SP
10, 20
Fryxellia D. M. Bates
Fuertesimalva Fryxell
Gaya H.B.K.
F
SP
G
8
5, 10, 15
6, 12
Gynatrix Alef.
Herissantia Medik.
Hochreutinera Krapov.
Hoheria A. Cunn
Horsfordia A. Gray
PG
HE
A
PG
BT
NA
6, 7
7
21
15
Howittia F. Muell.
Iliamna Greene
H
PH
NA
33
Kearnemalvastrum D. M.
Bates
Kitaibelia Willd.
Krapovickasia Fryxell
K
16
MA
SD
21, 22
8
Lavatera L. –Malva L.
M
7, 18, 20, 21, 22,
42, 56
Lawrencia Hook.
Lecanophora Speg.
Malacothamnus Greene
PG
G
PH
NA
6, 12,18
6, 17
Malope L.
Malvastrum A. Gray
Malvella Jaub. & Spach
MA
MS
SD
22, 25
6, 12, 18, 24
16?
Meximalva Fryxell
Modiola Moench
Modiolastrum K. Schum.
Monteiroa Krapov.
Napaea L.
Navaea Webb & Berthelot
Neobaclea Hochr.
Neobrittonia Hochr.
Nototriche Turcz.
Palaua Cav.
Periptera DC.
Phymosia Ham.
SD
MO
SP
SP
N
M
A
PH
SP
SP
AN
PH
8
9
5, 15, 50
10
15
21, 22?
8
16
5, 10, 15, 20
5
13
17
Plagianthus J.R. & G. Forst.
Pseudabutilon R.E. Fr.
PG
A
21
8, 16
7
S California, N Baja
California
W Texas, N Mexico
Andes, Mexico
Neotropics (Mexico, West
Indies, Bolivia)
Tasmania, S/SE Australia
Neotropics
Mexico, Paraguay/Argentina
New Zealand
SW US, N Mexico, Baja
California
SE Australia
W US & Canada,
Illinois/Virginia
Mexico to Costa Rica,
Columbia
E Europe
SW Texas & NE Mexico, S
Am
Mediterranean,
California/Baja California,
Australia
Australia
Temperate Argentina
California/Baja California &
Chile
Mediterranean
N Am & S Am
W US & Mexico, S Am,
Mediterranean
S Texas to C Mexico
Pantropical and subtropical
S Am
S Brazil, N Argentina
E United States
Macaronesia
Temperate Argentina
Mexico to Panama
Andes
Coastal Peru & Chile
W Mexico
Mexico, Guatemala,
Carribean
New Zealand
US to Argentina
Rhynchosida Fryxell
SD
8
Robinsonella Rose & Baker f.
Sida L. (outliers)
Sida L. s.s.
Sidalcea A. Gray
Sidasodes Fryxell & Fuertes
Sidastrum Baker f.
Sphaeralcea St.-Hil.
R
SD
SD
SC
SD
SD
SP
Tarasa Phil. (incl.
Urocarpidium)
Tetrasida Ulbr.
Wissadula Medik.
SP
16
8, 16
7, 14, 28
10, 20, 30
5
16
5, 10, 15,
25
5, 6, 10
SD
A
NA
7
8
S Texas & N Mexico, Bolivia &
Argentina
Mexico to Costa Rica
Pantropical
Pantropical
W United States and NW Mexico
Colombia, Eucador, Peru
Mexico & West Indies to Argentina
Temperate N & S Am
Andes, Mexico
Ecuador & Peru
Neotropics
Table 2.2.
Historical classification of genera currently placed in tribe Malveae (subfamily Malvoideae, Malvaceae)
Bentham and
Hooker 1862
Tribe Malveae
Schumann
Edlin
Kearney
Hutchinson
1890
1935
1949/1951a
1967
Tribe Malveae Tribe Malveae Tribe Malveae
Tribe Malveae
Subtribe
Subtribe
Subtribe
Subtribe Abutilinae
Subtribe Abutilinae
Abutilinae
Abutilinae
Corynabutilinae
Subtribe
Subtribe
Subtribe
Subtribe Malopinae
Subtribe Malvinae
Malvinae
Malvinae
Corynabutilinae
Subtribe
Subtribe
Subtribe Malvinae
Subtribe Malvinae
Tribe Abutileae
Sidinae
Sidinae
Subtribe Sidinae
Subtribe Sidinae Subtribe Abutilinae
Subtribe Sidinae
Tribe
Malopeae
Tribe
Malopeae
Tribe Malopeae
Tribe Malopeae
Bates
1968
Tribe Malveae
Bates and Blanchard
1970
Tribe Malveae
Fryxell
1988, pro parte
Tribe Malveae
Abutilon alliance
Abutilon alliance
Abutilon alliance
Anisodontea alliance
Anisodontea alliance
Anisodontea alliance
Anoda alliance
Anoda alliance
Anoda alliance
Gaya alliance
Howittia alliance
Kearnemalvastrum
alliance
Malacothamnus
alliance
Bakeridesia alliance
Callirhoe alliance
Bakeridesia alliance
Batesimalva alliance
Gaya alliance
Fryxellia alliance
Howittia alliance
Gaya alliance
Malope alliance
Malva alliance
9
Kearnemalvastrum
alliance
Malacothamnus
alliance
Malvastrum alliance
Malope alliance
Plagianthus alliance
Sidalcea alliance
Sphaeralcea alliance
Malva alliance
Malvastrum alliance
Napaea alliance
Plagianthus alliance
Sidalcea alliance
Sphaeralcea alliance
Herrisantia alliance
Howittia alliance
Kearnemalvastrum
alliance
Malope alliance
Malva alliance
Malvastrum alliance
Modiola alliance
Napaea alliance
Phymosia alliance
Plagianthus alliance
Robinsonella alliance
Sida alliance
Sidalcea alliance
Sphaeralcea alliance
Edlin (1935) and slightly modified by Kearney (1949, 1951) who erected a fourth
subtribe Corynabutilinae. In 1967, Hutchinson further restructured the family and tribes
by creating Abutileae (composed of subtribes Abutilinae and Sidinae), Malopeae, and
Malveae (subtribes Corynabutilinae and Malvinae). The tribe Abutileae was created to
accommodate genera with decurrent stigmas, while genera with apical stigmas remained
in the Malveae. In both tribes, the subtribes were distinguished by ovule number and
position. Bates (1968) completely revised the classification scheme by eliminating the
subtribes and, instead, created thirteen informal alliances within a single tribe Malveae.
These generic alliances were based on morphology and chromosome number that were
thought to reflect better phylogenetic affinities (Bates 1968). Bates and Blanchard (1970)
subsequently expanded this classification scheme to include sixteen alliances. The most
recent classification for the Malveae (in which palynological and comparative
morphology were also considered) was proposed by Fryxell (1988). Although only
Mexican genera were treated in this publication, several new alliances were created. The
major changes involved removing several genera from the Abutilon alliance and creating
three new alliances (Herrisantia, Robinsonella, and Sida), segregating Modiola from the
Sphaeralcea alliance into its own alliance, reassigning Callirhoe to the Sidalcea alliance,
renaming the Malacothamnus alliance as the Phymosia alliance, and creating two new
alliances for the newly described genera Batesimalva and Fryxellia. For the current
study, I have followed the taxonomy of Fryxell (1988) and have treated the non-Mexican
genera as in Bates and Blanchard (1970).
Recent molecular studies of the Malvales and the Malvoideae (as Malvaceae s.
str.) have provided preliminary evidence for phylogenetic relationships within the
subfamily as well as the tribe Malveae. The tribe Gossypieae was found to be sister to
the Malveae based on rbcL and atpB (Bayer et al. 1999) and ndhF (Alverson et al. 1999)
10
sequence data. However, a chloroplast DNA based phylogeny using RFLPs (La Duke
and Doebley 1995) placed the Malveae in a clade sister to the remaining tribes of the
family. Although La Duke and Doebley’s study did not support monophyly of the
Malveae alliances, it did indicate two major clades: one associated with the Abutilon and
Sida alliances and the other with the remaining alliances. A recent study (Fuertes Aguilar
et al. in press) based on ITS sequence data examined the phylogenetic relationships of the
Abutilon and Sida generic alliances. Although the sampling was not exhaustive, neither
alliance was supported as monophyletic (Fuertes Aguilar et al. in press).
Previous studies have demonstrated that sufficient variation exists in ITS to
resolve phylogenetic relationships within and between genera in the Malvaceae s. str.
(Seelanan et al. 1997) and particularly in the Malveae (Ray 1995; Andreasen and
Baldwin 2001; Whittall et al. 2000; Fuertes Aguilar et al. in press; and Chapter 3).
However, several of these studies have also revealed genera which are not monophyletic
as currently circumscribed. Among these are the Abutilon-Sida complex (Fuertes Aguilar
et al. in press) and the Malva-Lavatera group (Ray 1995). The main purpose of this
study was to reconstruct a phylogeny that would provide a framework for a discussion of
the diversity among Malveae genera. The traditional alliances and generic relationships
are here evaluated in the most comprehensive molecular-based phylogeny of the Malveae
to date.
MATERIALS AND METHODS
Taxon sampling
Sixty-three genera representing twenty of the twenty-one infratribal alliances
(Bates and Blanchard 1970, Fryxell 1988) were included.
Table 2.3 lists the taxa
included, their voucher and location or GenBank accession number if previously
11
published.
When possible, two or more species of the same genus were included.
Gossypium and Thespesia (tribe Gossypieae) were the designated outgroups.
DNA extraction and amplification
Leaf material was collected in the field and dried in silica gel (Chase and Hills
1991) or sampled from herbarium specimens.
In some cases, seeds removed from
herbarium specimens or collected in the field were grown in the greenhouse and fresh
leaf material was used. Total genomic DNA was extracted from fresh material by the
“rainforest” method of Scott and Playford (1996) or from herbarium material following
the modified small scale CTAB protocol (Doyle and Doyle 1987) of Loockerman and
Jansen (1996). The addition of a phenol extraction aided in obtaining clean DNA from
recalcitrant species (especially from older herbarium specimens).
The internal transcribed spacer (ITS) region of the 18S-25S nuclear ribosomal
repeat was chosen for phylogeny reconstruction. The ITS region was amplified by the
polymerase chain reaction (PCR) in a 50 uL cocktail consisting of 1x MasterAmp Tfl
PCR buffer (Epicentre), 2.5 µM MgCl2, 0.8 µM DNTPs, 0.05 µM DMSO, 0.5 unit TFL
polymerase (Epicentre) and 0.4 µM forward and reverse primers. Amplification primers
used were 7A (A. Plovanovich and J. Panero unpublished) and 4 (White et al. 1990) or
for ITS 1 and ITS 2 separately, primers 7A and 2 (Kim and Jansen 1994) and primers 3
(Kim and Jansen 1994) and 4, respectively. After an initial denaturing step at 95° for 5
minutes (“hot start”), the reactions were held at 72° while polymerase was added to each
reaction. Amplification of the ITS proceeded at 94° for 3 min, 46-52° for 1 min., 72° for
1 min., followed by 35 cycles of 94° 1 minute, 46-52° 1 minute, 72° for 45 sec. + 3
sec/cyc. with a final 7-15 minute extension at 72°.
12
Amplification products were
Table 2.3.
List of included taxa, voucher information (or GenBank accession number), and collection locality information.
*Where two accession numbers occur, the first corresponds to the ITS1 sequence and the second to ITS2. JL
indicates DNA provided by John La Duke (University of North Dakota), JFA was provided by Javier Fuertes
Aguilar (Real Jardin Botanico, Madrid), and TBS indicates DNA provided by Tracey Bodo Slotta.
Species
Abutilon andrewsianum W. Fitzg.
Abutilon pubistamineum Ulbr.
Abutilon theophrasti Medik.
Acaulimalva alismatifolia (K. Schum. & Hieron.)
Krapov.
Acaulimalva dryadifolia (Solms) Krapov.
Alcea rosea Linn.
Alcea rugosa Alef.
Allosidastrum pyramidatum (Cav.) Krapov., Fryx.,
D.M. Bates
Allowissadula holosericea (Scheele) D.M. Bates
Anisodontea capensis (L.) D.M. Bates
Anoda crenatiflora Orteg.
Bakeridesia gloriosa D.M. Bates
Bastardia bivalvis Kunth
Bastardiastrum cinctum (Brandegee) D.M. Bates
Bastardiopsis densiflora Hassl.
Batesimalva violacea (Rose) Fryx.
Billieturnera helleri (Rose ex Heller) Fryx.
Briquetia sonorae Fryx.
Briquetia spicata (Kunth) Fryx.
Callirhoe digitata Nutt.
Callirhoe involucrata A. Gray
Calyculogygas uruguayensis Krapov.
Voucher*
Locality
Johnson and Thomas MRS 351 (TEX) Australia - Queensland
AJ251049/AJ274993
La Duke s.n. (GFND)
USA - Minnesota – St. Paul
Sagástegui A. et al. 15772 (TEX)
Peru - Cajamarca - Cajamarca
Lewis 37150 (TEX)
AF303022
AJ251164/AJ251170
AJ274965/AJ274996
AJ274963/AJ274994
AJ251165/AJ251171
AJ251043/AJ274987
Panero et al. 5968 (TEX)
Werling 293 (LL)
Van Devender 98-522 (TEX)
Arbo et al. 5902 (TEX)
Powell and Powell 3632 ((TEX)
JL
Felger and Straub 85-1315 (TEX)
Guillén and Roca 3557 (TEX)
AJ304936
Tate s.n. (TEX)
Krapovickas and Cristobal 16169 (LL)
13
Bolivia - La Paz - Inquisivi
Mexico - Oaxaca - Tlacolula
Mexico - Oaxaca - Tlacolula
Mexico – Sonora – Mcpio. San Javier
Argentina - Misiones - San Javier
USA - Texas - Brewster county
Mexico – Sonora (Cañon Nacapules)
Bolivia – Santa Cruz – Velasco
USA - Texas
Uruguay - Lavalleja
Corynabutilon vitifolium (Cav.) Kearney
Cristaria andicola Gay
Dendrosida breedlovei Fryx.
Dendrosida wingfieldi Fryx.
Dirhamphis sp.
Eremalche exilis Greene
Eremalche parryi Greene
Fryxellia pygmaea (Correll) D.M. Bates
Fuertesimalva jacens (S. Watson) Fryx.
AJ274970/AJ275001
Tate et al. 97 (TEX)
AJ251032/AJ274976
AJ251033/AJ274977
JL
Morefield and McCarty 3395 (TEX)
AJ304938
JL
Koch and Fryxell 77326 (LL)
Fuertesimalva limensis (L.) Fryx.
Gaya atiquipana Krapov.
Gaya calyptrata H.B. & K.
Gossypium hirsutum Cav.
Hochreutinera amplexifolia (DC.) Fryx.
Hoheria sp.
Tate and Timana 60 ((TEX)
Tate et al. 106 (TEX)
AJ251048/AJ274992
U12719
Rubio 1087 (TEX)
JFA
Horsfordia sp.
Howittia trilocularis F. Muell.
JL
Craven s.n. (TEX)
Iliamna bakeri (Jepson) Wiggins
Iliamna lactibracteata Wiggins
Iliamna remota Greene
Iliamna rivularis Greene
Kearnemalvastrum sp.
Kearnemalvastrum lacteum (Aiton) D. M. Bates
Kitaibelia vitifolia Willd.
Krapovickasia physaloides (Presl) Fryx.
Lavatera arborea L.
Lavatera occidentalis S. Watson
Lavatera trimestris L.
Lecanophora chubutensis (Speg.) Rodrigo
AF271166
AF271183
AF271196
AF271201
JL
Calzada 21569 (TEX)
AJ274972/AJ275003
AJ251047/AJ274991
AF303016
AJ251167/AJ251173
AF303027
Tate et al. 72 (TEX)
14
Chile - II Reg. de Antofagasta - El Loa
USA - California - Inyo county
Mexico - Mexico – Mcpio. Tenango de
Arista
Peru - Ancash - Yungay
Peru – Arequipa - Caraveli
Mexico - Queretaro - Mcpio. Landa
Cultivated - Real Jardin Botanico,
Madrid
Cultivated - Australian National Botanic
Garden, Canberra
Mexico – Oaxaca – Mcpio. Santiago
Argentina - Mendoza - Las Heras
Lecanophora heterophylla (Cav.) Krapov.
Malacothamnus chilensis (Gay) Krapov.
Malacothamnus fasciculatus Greene
Tate et al. 70 (TEX)
Meudt and Lopez 028 (TEX)
TBS
Malope sp.
Malva assurgentiflora (Kellogg) M.F. Ray
Malva dendromorpha M.F. Ray
Malva linnaei M.F. Ray
Malva nicaensis All.
Malva wigandii (Alef.) M.F. Ray
Malvastrum amblyphyllum R.E. Fries
Malvastrum americanum (L.) Torr.
Malvastrum coromandelianum (L.) Garcke
Malvella sagittifolia (A. Gray) Fryx.
Meximalva filipes (A. Gray) Fryx.
Modiola caroliniana (L.) G. Don
Modiolastrum lateritium (Hook.) Krapov.
Monteiroa glomerata (Hook. et Arn.) Krapov.
JFA
AF303032
AF303020
AF303015
AF303028/AF303029
AF303019
Krapovickas et al. 47237 (TEX)
Nee 50549 (TEX)
AJ274971/AJ275002
AJ251045/AJ274989
AJ251039/AJ274983
Tate et al. 50 (TEX)
Tressens et al. 4592 (TEX)
Krapovickas and Cristobal 43703
(TEX)
AJ304940
AF303030
JFA
Tate et al. 85 (TEX)
Funk and Bernal H. 11289 (TEX)
Tate et al. 102 (TEX)
Tate et al. 103 (TEX)
Tate et al. 105 (TEX)
Torres and Ramirez 13674 (TEX)
JL
AJ274964/AJ274995
AJ251046/AJ274990
JL
Napaea dioica Linn.
Navaea phoenicea Webb & Berth.
Neobrittonia acerifolia Hochr.
Nototriche anthemidifolia A.W. Hill
Nototriche flabellata A.W. Hill
Nototriche pedicularifolia (Meyen) Hill
Palaua camanensis R. Ferreyra & M. Chanco
Palaua rhombifolia R. Grah.
Periptera punicea DC.
Phymosia umbellata (Cav.) Kearney
Pseudabutilon umbellatum (L.) Fryx.
Rhynchosida physocalyx (A. Gray) Frxy.
Robinsonella sp.
15
Argentina - Mendoza - Las Heras
Chile – Santiago – Prov. de Cordillera
Rancho Santa Ana Botanical Garden
14242
Argentina – Formosa – Bermejo
Bolivia – Santa Cruz – Warnes
Mexico - Puebla
Argentina - Misiones - Guaraní
Brasil - Santa Catarina
Argentina - Salta - Santa Victoria
Bolivia - La Paz - Murillo
Peru - Arequipa - Arequipa
Peru - Arequipa - Islay
Peru - Arequipa - Caraveli
Mexico – Michoacán – Mcpio. Zitácuaro
Sida abutifolia Mill.
Sida aggregata Presl
Sida fibulifera Lindl.
Sida hermaphrodita (L.) Rusby
Sida hookeriana Miq.
Sida juissieana DC.
Sida linifolia Cav.
Sida longipes A. Gray
Sida odorata Monteiro
Sida oligandra K. Schum.
Sida platycalyx Benth.
Sida xantii A. Gray
Sidalcea cusickii Piper
Sidalcea hirsuta A. Gray
Sidalcea multifida Greene
Sidalcea stipularis J.T. Howell & True
Sidasodes colombiana Fryx. & J. Fuertes
Sidastrum paniculatum (L.) Fryx.
Sphaeralcea angustifolia G. Don
Sphaeralcea cordobensis Krapov.
Sphaeralcea crispa Hook. ex Baker f.
Sphaeralcea philippiana Krapov.
Sphaeralcea wrightii A. Gray
Tarasa albertii Phil.
Tarasa humilis (Gill. ex Hook. et Arn.) Krapov.
Tarasa machupicchensis Krapov.
Tarasa mandonii (Baker) Kearney
Tarasa operculata (Cav.) Krapov.
Tarasa rhombifolia Krapov.
Tarasa thyrsoidea Krapov.
Tarasa trisecta (Griseb.) Krapov.
Tetrasida chachapoyensis (Baker f.) Fryx. & J. Fuertes
AJ274961/AJ251617
AJ274943/AJ251599
AJ251042/AJ274986
JFA
JFA
AJ251036/ AJ274980
AJ274959/AJ251613
AJ251030/AJ274974
AJ274960
AJ274966/AJ274997
AJ251041/AJ274985
AJ274946/AJ251602
AF196546
AJ304896
AJ304910
AJ304932
AJ274969/AJ275000
AJ251040/AJ274984
Tate et al. 51 (TEX)
Tate et al. 79 (TEX)
Tate et al. 77 (TEX)
Tate et al. 71 (TEX)
Tate et al. 53 (TEX)
Tate et al. 74 (TEX)
Tate et al. 69 (TEX)
Timana and Tate 3771 (TEX)
Beck 14510 (TEX)
Tate and Timaná 62 (TEX)
Tate and Symonds 109 (TEX)
Tate et al. 107 (TEX)
Tate et al. 91 (TEX)
Campos and Diaz 2369 (TEX)
16
Mexico - San Luis Potosi
Argentina - Cordoba
Argentina - La Pampa - Caleu Caleu
Argentina - Mendoza - Las Heras
Mexico - Tamaulipas
Argentina - Mendoza - Malargüe
Chile - IX Reg. de Araucania
Peru - Cuzco - Urubamba
Bolivia - Cochabamba - Ayopaya
Peru - Arequipa - Arequipa
Peru - Cuzco - Urubamba
Peru - Arequipa - Caraveli
Argentina - Salta - Cafayate
Peru - Cajamarca - San Ignacio
Tetrasida weberbaueri (Ulbr.) Fryx. & J. Fuertes
Thespesia lampas (Cav.) Dalzell ex Dalzell & A.
Gibson
Urocarpidium albiflorum Ulbr.
Wissadula boliviana R.E. Fries
Wissadula cruziana R.E. Fries
Wissadula periplocifolia Griseb.
Campos and Diaz 2367 (TEX)
U56779
Peru - Cajamarca - San Ignacio
Burandt 2340 (F)
Nee 48628 (TEX)
Nee 47523 (TEX)
Hill and Horn 27076 (TEX)
Peru - Moquegua
Bolivia - Santa Cruz - Andrés Ibáñez
Bolivia - Santa Cruz - Cordillera
Guyana - Region IV - DemeraraMahaica district
17
separated on a 1% agarose gel, stained with ethidium bromide and visualized with UV on
a transilluminator. PCR products were cleaned using Qiagen QIAquick spin columns
following manufacturer’s instructions. Cycle sequencing was performed using Big Dye
terminator chemistry. Centri-sep sephadex columns were used to remove residual salts
and unincorporated nucleotides from the single-stranded products.
Automated
sequencing using the forward and reverse amplification primers was conducted on an
ABI 3700 at the DNA Analysis Laboratory at the University of Texas at Austin.
Sequence analysis
The boundaries of ITS were determined by comparison to published Gossypium
sequences.
Complementary strands were edited using Sequencher (Gene Codes
Corporation 1995). The sequences were aligned using Clustal X (Thompson et al. 1997)
with manual adjustments as needed.
Sequences were examined for angiosperm-
conserved regions in ITS1 (Liu and Schardl 1994) and ITS2 (Hershkovitz and Zimmer
1996). These regions were used to align taxa and if sequences were found which did not
maintain these regions, they were considered to be paralogous copies and were discarded
from the phylogenetic analyses. Sequence length and GC content were calculated in
MacClade 4.0 (Maddison and Maddison 2000). Pairwise divergence values and the
presence of biased base composition were assessed using PAUP* (Swofford 2002).
Phylogenetic analysis
Because the 5.8S was not available for all sequences, the region was excluded
from the phylogenetic analyses.
Maximum parsimony was used as the optimality
criterion in PAUP* version 4.0b10 (Swofford 2002).
The data set was tested for
hierarchical structure by evaluating 10000 randomly generated trees (Hillis and
18
Huelsenbeck 1992). For phylogeny reconstruction, an heuristic search was employed
with 1000 random addition sequences, TBR branch swapping, ACCTRAN characterstate optimization and gaps coded as missing. To reduce the amount of time spent
swapping on suboptimal trees, only 5 trees were held at each replicate. The best trees
were then swapped to completion. Support for monophyly of the clades was determined
by 100 bootstrap replicates (Felsenstein 1985).
RESULTS
Sequence characteristics and phylogenetic reconstruction
One hundred sixteen sequences (65 of these new) were analyzed for Malveae taxa
representing twenty (Herrisantia alliance not represented) of the twenty-one infratribal
alliances (Appendix A).
These sequences will be deposited in GenBank prior to
publication. ITS1 length varied between taxa from 240 to 296 base pairs (bp), while
ITS2 contained 184 to 232 bp in the included taxa. Mean GC content of ITS1 and ITS2
was 46.9 – 61.8% and 49.8 – 67.7%, respectively. Pairwise divergence between genera
ranged from 0.021-0.213. The aligned region including ITS1 and ITS2 contained 622
characters:
137 characters were constant, 95 were autapomorphic, and 390 were
parsimony informative. ITS1 contained 225 parsimony informative characters while
ITS2 contained 165. Evaluating 10000 random trees returned a g1 statistic of -0.315
[critical value for 25 taxa and 500 informative characters = -0.08 (0.05 level) and –0.09
(0.01 level)] which indicates significant structure to the ITS data set (Hillis and
Huelsenbeck 1992).
An heuristic search as described above recovered 53 most parsimonious (MP)
trees of length 2992 with a CI= 0.2920 (excluding autapomorphies), RI= 0.6562, and
RC=0.2112. Figure 2.1 is one of these MP tree (that is similar to the majority rule tree)
19
and Figure 2.2 is the strict consensus of the most parsimonious trees. Figures 2.3 and 2.4
highlight the alliances and genera of clades A and B, respectively, on one most
parsimonious tree (same as Fig. 2.1) (see discussion below).
Table 2.4
Analysis of ITS regions for taxa included in this study. Length and GC
content were calculated using MacClade v. 4.0 (Maddison and Maddison
2000). Pairwise divergence values and number of parsimony informative
characters were found using PAUP* v.4.0b8 (Swofford 2002).
Region
ITS1
ITS2
Table 2.5
Length
GC content
Parsimony informative
characters
240-296 46.9 – 61.8 %
225
184-232 49.8 – 67.7 %
165
Test for base composition bias of analyzed sequences (for entire data set
including outgroups). Values given are mean base frequency, degrees of
freedom (df), and P value (a value greater than 0.05 indicates that there is
not a significant difference among the taxa in the data set).
Region
ITS
A
0.21927
C
0.27938
20
G
0.27115
T
0.23020
df
372
P
0.990
10
7
3
1
5
14
9
27
22
19
13
34
10
12
18
31
3
34
55
45
16
3
0
6
9
21
8
7
40
9
12
11
4
16
27
4
11
10
8
3
7
29
16
12
6
1
12
9
16
Clade B
8
11
3
32
5
3
3
57
2
3
11
18
12
12
23
5
20
0
3
0
3
7
4
16
3
9
17
4
13
3
8
2
5
8
3
3
3
2
3
4
13
8
8
2
0
4
4
2
0
3
7
22
5
3
11
1
2
3
1
2
11
6
12
2
8
1
3
2
3
6
7
10
3
10
9
12
19
42
32
21
16
7
21
18
9
14
6
6
15
22
9
0
1
13
17
22
7
50
17
4
6
30
5
21
5
7
22
13
22
11
4
1
17
9
3
7
2
30
5
8
10
8
11
42
31
5
31
43
14
33
21
0
18
7
14
20
15
23
7
14
Clade A
6
1
28
6
8
8
9
15
13
40
12
6
28
21
31
14
32
60
41
19
12
9
14
17
16
14
9
18
62
15
28
8
21
24
35
13
18
5
9
15
25
105
50
52
Figure 2.1
Malva.nicaensis
Malva.linnaei
Lavatera.occidentalis
Malva.assurgentea
Malva.dendromorpha
Malva.wigandii
Lavatera.arborea
Lavatera.trimestris
Navaea.phoenicea
Malope.sp.
Kitaibela.vitifolia
Alcea.rosea
Alcea.rugosa
Callirhoe.digitata
Callirhoe.involucrata
Napaea.dioica
Anisodontea.capensis
Malacothamnus.fasciculatus
Phymosia.umbellata
Iliamna.rivularis
Iliamna.remota
Palaua.rhombifolia
Palaua.camanensis
Urocarpidium.albiflorum
Fuertesimalva.jacens
Fuertesimalva.limensis
Sidalcea.cusickii
Sidalcea.multifida
Sidalcea.hirsuta
Sidalcea.stipularis
Eremalche.exilis
Eremalche.parryi
Iliamna.bakeri
Iliamna.latibracteata
Malvastrum.americanum
Malvastrum.amblyphyllum
Malvastrum.coromandelianum
Monteiroa.glomerata
Modiolastrum.lateritium
Modiola.caroliniana
Calyculogygas.uruguayensis
Kearnemalvastrum.sp.
Kearnemalvastrum.lacteum
Acaulimalva.dryadifolia
Acaulimalva.alismatifolia
Malacothamnus.chilensis
Tarasa.mandonii
Tarasa.machupicchensis
Sphaeralcea.angustifolia
Sphaeralcea.wrightii
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.cordobensis
Tarasa.operculata
Tarasa.thyrsoidea
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Tarasa.trisecta
Tarasa.rhombifolia
Tarasa.humilis
Tarasa.albertii
Sidastrum.paniculatum
Meximalva.filipes
Sida.platycalyx
Sida.fibulifera
Anoda.crenatiflora
Periptera.punicea
Horsfordia.exsita
Bakeridesia.gloriosa
Bastardiastrum.cinctum
Wissadula.cruziana
Wissadula.boliviana
Wissadula.periplocifolia
Tetrasida.chachapoyensis
Pseudabutilon.umbellatum
Abutilon.pubistamineum
Bastardia.bivalvis
Bastardiopsis.densiflora
Abutilon.Laduke
Abutilon.andrewsianum
Hochreutinera.amplexifolia
Briquetia.sonorae
Dirhamphis.sp.
Gaya.calyptrata
Gaya.atiquipana
Billieturnera.helleri
Robinsonella.sp.
Sida.oligandra
Fryxellia.pygmaea
Dendrosida.breedlovei
Dendrosida.wingfieldi
Sida.abutifolia
Sida.odorata
Sida.linifolia
Sida.aggregata
Sida.xantii
Sida.longipes
Sida.jussieana
Krapovickasia.physaloides
Rhynchosida.physocalyx
Neobrittonia.acerifolia
Batesimalva.violacea
Allowissadula.holosericea
Tetrasida.weberbaueri
Allosidastrum.pyramidatum
Malvella.sagittifolia
Corynabutilon.vitifolium
Lecanophora.chubutensis
Lecanophora.heterophylla
Cristaria.andicola
Sidasodes.colombiana
Sida.hookeriana
Hoheria.sp.
Sida.hermaphrodita
Howittia.trilocularis
Gossypium.hirsutum
Thespesia.lampas
Malva
Malope
Malva
Sidalcea
Napaea
Anisodontea
Phymosia
Sphaeralcea
Sidalcea
Sphaeralcea
Phymosia
Malvastrum
Sphaeralcea
Kearnemalvastrum
Sphaeralcea
Phymosia
Sphaeralcea
Sida
Anoda
Batesimalva
Bakeridesia
Abutilon
Sida
Abutilon
Batesimalva
Gaya
Abutilon
Robinsonella
Sida
Fryxellia
Sida
Phymosia
Batesimalva
Abutilon
Sida
Abutilon
Gaya
Sida
Plagianthus
Sida
Howittia
One of 53 most parsimonious trees based on ITS sequence data
[length=2992, CI= 0.2920 (excluding autapomorphies), RI= 0.6562, and
RC=0.2112]. Branch lengths are shown above the branches. Alliance
groupings are shown at right.
21
95
96
99
100
100
100
77
72
75
94
100
100
100
99
92
98
94
100
93
76
100
91
84
87
68
96
63
100
100
93
98
90
62
79
100
100
73
99
72
100
68
76
99
100
99
99
66
84
71
100
100
100
74
100
82
.
88
100
57
100
52
58
100
51
84
75
100
96
73
51
100
100
77
100
53
99
51
100
Figure 2.2
83
Malva.nicaensis
Malva.linnei
Lavatera.occidentalis
Malva.assurgentea
Malva.dendromorpha
Malva.wigandii
Lavatera.arborea
Lavatera.trimestris
Navaea.phoenicea
Malope.sp.
Kitaibela.vitifolia
Alcea.rosea
Alcea.rugosa
Callirhoe.digitata
Callirhoe.involucrata
Napaea.dioica
Anisodontea.capensis
Malacothamnus.fasciculatus
Iliamna.rivularis
Iliamna.remota
Phymosia.umbellata
Palaua.rhombifolia
Palaua.camanensis
Urocarpidium.albiflorum
Fuertesimalva.jacens
Fuertesimalva.limensis
Sidalcea.cusickii
Sidalcea.multifida
Sidalcea.hirsuta
Sidalcea.stipularis
Eremalche.exilis
Eremalche.parryi
Iliamna.bakeri
Iliamna.latibracteata
Malvastrum.americanum
Malvastrum.amblyphyllum
Malvastrum.coromandelian
Monteiroa.glomerata
Modiolastrum.lateritium
Modiola.caroliniana
Calyculogygas.uruguayensis
Kearnemalvastrum.sp.
Kearnemalvastrum.lacteum
Acaulimalva.dryadifolia
Acaulimalva.alismatifolia
Malacothamnus.chilensis
Tarasa.mandonii
Tarasa.machupicchensis
Sphaeralcea.angustifolia
Sphaeralcea.wrightii
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.cordobensis
Tarasa.operculata
Tarasa.thyrsoidea
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Tarasa.trisecta
Tarasa.rhombifolia
Tarasa.humilis
Tarasa.albertii
Sidastrum.paniculatum
Meximalva.filipes
Sida.platycalyx
Sida.fibulifera
Anoda.crenatiflora
Periptera.punicea
Horsfordia.exsita
Bakeridesia.gloriosa
Bastardiastrum.cinctum
Wissadula.cruziana
Wissadula.boliviana
Wissadula.periplocifolia
Tetrasida.chachapoy
Pseudabutilon.umbellatum
Abutilon.pubistamineum
Bastardia.bivalvis
Bastardiopsis.densiflora
Abutilon.Laduke
Abutilon.andrewsianum
Briquetia.sonorae
Hochreutinera.amplexifolia
Dirhamphis.sp.
Gaya.calyptrata
Gaya.atiquipana
Billieturnera.helleri
Robinsonella.sp.
Sida.oligandra
Fryxellia.pygmaea
Dendrosida.breedlovei
Dendrosida.wingfieldi
Sida.aggregata
Sida.xantii
Sida.abutifolia
Sida.odorata
Sida.linifolia
Sida.longipes
Sida.jussieana
Krapovickasia.physaloides
Rhynchosida.physocalyx
Neobrittonia.acerifolia
Batesimalva.violacea
Allowissadula.holosericea
Tetrasida.weberbaueri
Allosidastrum.pyramidatum
Malvella.sagittifolia
Corynabutilon.vitifolium
Lecanophora.chubutensis
Lecanophora.heterophylla
Cristaria.andicola
Sidasodes.colombiana
Sida.hookeriana
Hoheria.sp.
Sida.hermaphrodita
Howittia.trilocularis
Gossypium.hirsutum
Thespesia.lampas
The strict consensus of 53 most parsimonious trees based on ITS sequence
data. Bootstrap support (above 50) for 100 replicates is indicated below the
nodes.
22
10
21
100 9
12
9
99 19
18
99
42
14
32
6
21
6
66 16
22
15
84
9
71
7
Clade B
0
22
100 1
13
6
74 17
17
100
4
30
5
21
5
22
17
100 13
22
3
11
9
82
7
7
8
57
4
2
1
30 88
100
5
42
100
31
10
52
43
100
8
5
11
31
14
58
43
33
8
6
28
6 100 1
21
0
18
51
14
96
9
13
51
14
7
73
7
75
20
15
8
84
15
23
40
12
41
100
9
100
6
19
100
12
77
9
28
21
31
17
14
14
100 14
32
16
53 60
18
8
62
21
99
15
28
24
35
13
51
9
5
18 83 15
100
25
105
49
50
Figure 2.3
Sidastrum.paniculatum
Meximalva.filipes
Sida.platycalyx
Sida.fibulifera
Anoda.crenatiflora
Periptera.punicea
Horsfordia.exsita
Bakeridesia.gloriosa
Bastardiastrum.cinctum
Wissadula.cruziana
Wissadula.boliviana
Wissadula.periplocifolia
Tetrasida.chachapoyensis
Pseudabutilon.umbellatum
Abutilon.pubistamineum
Bastardia.bivalvis
Bastardiopsis.densiflora
Abutilon.Laduke
Abutilon.andrewsianum
Hochreutinera.amplexifolia
Briquetia.sonorae
Dirhamphis.sp.
Gaya.calyptrata
Gaya.atiquipana
Billieturnera.helleri
Robinsonella.sp.
Sida.oligandra
Fryxellia.pygmaea
Dendrosida.breedlovei
Dendrosida.wingfieldi
Sida.abutifolia
Sida.odorata
Sida.linifolia
Sida.aggregata
Sida.xantii
Sida.longipes
Sida.jussieana
Krapovickasia.physaloides
Rhynchosida.physocalyx
Neobrittonia.acerifolia
Batesimalva.violacea
Allowissadula.holosericea
Tetrasida.weberbaueri
Allosidastrum.pyramidatum
Malvella.sagittifolia
Corynabutilon.vitifolium
Lecanophora.chubutensis
Lecanophora.heterophylla
Cristaria.andicola
Sidasodes.colombiana
Sida.hookeriana
Hoheria.sp.
Sida.hermaphrodita
Howittia.trilocularis
Gossypium.hirsutum
Thespesia.lampas
Sida
Anoda
Batesimalva
Bakeridesia
Abutilon
Sida
Abutilon
Batesimalva
Gaya
Abutilon
Robinsonella
Sida
Fryxellia
Sida
Phymosia
Batesimalva
Abutilon
Sida
Abutilon
Gaya
Sida
Plagianthus
Sida
Howittia
Clade A from one most parsimonious tree (see Figure 2.1). Branch lengths
are shown above the branches and bootstrap support (above 50) for 100
replicates is given below. Genera are colored according to their alliance
associations which also correspond to the bars on the right.
23
19
100
22
100
7
3
1
9
99
27
34
13
100
12
31
72
10
77
3
75
10
5
95
14
96
18
34
55
45
16
94
8
6
40
100 9
12
9
7
3
0
21
100
11
4
16
27
3
7
4
29
100 11
10
12
8
99
16
6
1
12
9
92
0
7
98
3
0
3
4
11
76
3
16
16 100
93
3
11
18
5
91
3
57
84
2
23
100
5
20
8
32
12
12
3
3
9
87
17
13
4
3
2
5
68
8
8
3
3
96
4
63
3
3
2
13
100
8
8
100
2
0
3
93
4
7
98
5
90
22
62
11
100
0
1
2
11
100
6
73
12
99
Clade A
3
3
76
8
1 100
68
3
1
2
2
72
2
6
7
7
99
Figure 2.4
4
2
3
79
10
3
Malva.nicaensis
Malva.linnei
Lavatera.occidentalis
Malva.assurgentea
Malva.dendromorpha
Malva.wigandii
Lavatera.arborea
Lavatera.trimestris
Navaea.phoenicea
Malope.sp.
Kitaibela.vitifolia
Alcea.rosea
Alcea.rugosa
Callirhoe.digitata
Callirhoe.involucrata
Napaea.dioica
Anisodontea.capensis
Malacothamnus.fasciculatus
Phymosia.umbellata
Iliamna.rivularis
Iliamna.remota
Palaua.rhombifolia
Palaua.camanensis
Urocarpidium.albiflorum
Fuertesimalva.jacens
Fuertesimalva.limensis
Sidalcea.cusickii
Sidalcea.multifida
Sidalcea.hirsuta
Sidalcea.stipularis
Eremalche.exilis
Eremalche.parryi
Iliamna.bakeri
Iliamna.latibracteata
Malvastrum.americanum
Malvastrum.amblyphyllum
Malvastrum.coromandelianum
Monteiroa.glomerata
Modiolastrum.lateritium
Modiola.caroliniana
Calyculogygas.uruguayensis
Kearnemalvastrum.sp.
Kearnemalvastrum.lacteum
Acaulimalva.dryadifolia
Acaulimalva.alismatifolia
Malacothamnus.chilensis
Tarasa.mandonii
Tarasa.machupicchensis
Sphaeralcea.angustifolia
Sphaeralcea.wrightii
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.cordobensis
Tarasa.operculata
Tarasa.thyrsoidea
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Tarasa.trisecta
Tarasa.rhombifolia
Tarasa.humilis
Tarasa.albertii
Malva
Malope
Malva
Sidalcea
Napaea
Anisodontea
Phymosia
Sphaeralcea
Sidalcea
Sphaeralcea
Phymosia
Malvastrum
Sphaeralcea
Kearnemalvastrum
Sphaeralcea
Phymosia
Sphaeralcea
Clade B from one most parsimonious tree (see Figure 2.1). Branch lengths
are shown above the branches and bootstrap support (above 50) for 100
replicates is given below. Genera are colored according to their alliance
associations which also correspond to the bars on the right.
24
DISCUSSION
The traditional alliances (as well as several genera) are not monophyletic
Consistent with a previous chloroplast-based phylogeny (La Duke and Doebley
1995), the informal alliances of the Malveae are not monophyletic in the ITS phylogeny
(Figs. 2.1, 2.3, 2.4). Figures 2.1, 2.3, and 2.4 illustrate the general distribution of the
alliances on one most parsimonious tree. Overall, two main clades were reconstructed:
one clade (hereafter referred to as Clade A) containing the Abutilon, Bakeridesia,
Batesimalva, Fryxellia, Gaya, Plagianthus, Robinsonella, and Sida alliances; and a
second large clade (Clade B) consisting of genera from the Anisodontea, Callirhoe,
Kearnemalvastrum, Malva, Malvastrum, Modiola, Napaea, Phymosia, Sphaeralcea, and
Sidalcea alliances. Although many more genera were included here, the structure of the
ITS tree appears congruent with the cp-DNA tree of La Duke and Doebley (1995) in that
two main clades were reconstructed. None of the historical classification schemes are
reflected in the ITS phylogeny, however (Table 2.2). While some alliances are not
monophyletic, only one alliance (Phymosia) appeared in the two main clades. Therefore,
the alliances in general seem to indicate better generic affinities than the previous
subtribal classifications.
In this case, chromosome number and general geographic
distribution seem to be more reliable indicators of phylogenetic relatedness than are some
of the previously emphasized morphological characters (particularly those of the carpel).
The tribe Malveae appears to be monophyletic, although sampling did not include
three of the other four tribes in the Malvoideae. To place the tribe definitively within the
family, sampling should include members of the tribes Dechaschistieae, Gossypieae,
Hibisceae, and Malvavisceae. However, as the ITS alignment even within the tribe
presented difficulties, the use of a more slowly evolving molecule for phylogeny
25
reconstruction would permit the inclusion of more distantly related taxa. Likewise, the
high homoplasy levels (CI=0.2920 excluding uninformative characters, RC=0.2112)
indicate that this marker may not be particularly well-suited for a tribal level phylogeny.
However, homoplasy levels have been shown to increase when a large number of taxa are
analyzed (Sanderson and Donoghue 1989).
The basal-most lineage of the Malveae is occupied by Howittia, a monotypic
genus from southeastern Australia. The systematic position of this genus has been
questionable for some time. Bentham and Hooker (1862) first placed the genus in the
subtribe Abutileae with other genera containing two to many ovules per carpel (e.g.,
Abutilon, Sphaeralcea, Modiola). Edlin (1935) proposed that perhaps this genus did not
belong in the Malveae since it possesses morphological features rather atypical for the
tribe (shrubby habit, staminal column antheriferous to the base, lack of stylar branches,
and non-disarticulating fruits). Instead, the tribe Hibisceae was thought to house this
unusual genus better (Edlin 1935). Bates (1968) placed Howittia in its own alliance
within the Malveae and allied the genus to other genera in the Abutilon alliance. Since
Howittia occupies a basal (or perhaps sister) lineage of the Malveae, it is particularly
unfortunate that the chromosome number is unknown (Bates [1968] suggested a base
chromosome number of 7 or 8 for the tribe).
Several genera were also resolved as non-monophyletic in the ITS phylogeny:
Abutilon, Iliamna, Lavatera, Malva, Malacothamnus, Sida, Tarasa, and Tetrasida.
Previous phylogenetic studies of the Abutilon-Sida complex (Fuertes Aguilar et al. in
press) and the Lavatera-Malva group (Ray 1995) similarly demonstrated a lack of
monophyly for these four genera. The issue of non-monophyly for the remaining genera
was unexpected. For the remainder of the discussion I will focus on the overall pattern of
alliance and generic relatedness within clades A and B, respectively.
26
Alliances and genera of Clade A
As previously mentioned, Clade A contains mostly members of the Sida and
Abutilon alliances, along with genera from the Bakeridesia, Fryxellia, Gaya, Phymosia,
and Robinsonella alliances (Figure 2.3). The Abutilon-Sida complex was the subject of a
recent phylogenetic investigation using ITS (Fuertes Aguilar et al. in press) in which the
two genera were found to be non-monophyletic.
Sida (100 spp.) has long been
recognized as a heterogeneous assemblage (Fryxell 1985). Attempts to create a more
natural group have resulted in several segregate genera: Allosidastrum, Bastardiopsis,
Billieturnera,
Dendrosida,
Krapovickasia,
Malvella,
Meximalva,
Rhynchosida,
Sidastrum, and Tetrasida. Consequently, it is not surprising that the remaining species of
Sida as well as the entire Sida alliance (12 genera) still do not form a monophyletic group
in this expanded ITS phylogeny (Figure 2.3). A “core” Sida clade was reconstructed,
which consisted of Sida abutifolia (section Spinosae), S. aggregata (section Muticae), S.
linifolia (section Stenindae), S. longipes (section Ellipticifoliae), S. jusseiana (section
Nelavagae), S. xanti (section Cordifoliae) along with Dendrosida.
Fryxell (1997)
suggested Meximalva and Dendrosida were potential close relatives to Sida. In fact, both
genera are closely related to species of Sida although they occur in separate clades. The
remaining species of Sida are distributed throughout Clade A (Figure 2.3). Likewise, the
11 genera of the Abutilon alliance are scattered throughout clade A. The three species of
Abutilon (200 spp.) included here also did not form a monophyletic group. The potential
for non-monophyly appears high for alliances which are composed of many genera (e.g.,
Abutilon, Sida, Sphaeralcea). However, even a few of the smaller alliances are not
monophyletic in the ITS phylogeny: Gaya alliance (3 genera), Batesimalva alliance (4
genera), and the Phymosia alliance (1 sp. in clade A, the other 3 in clade B). In
particular, the placement of Neobrittonia of the Phymosia alliance (Fryxell 1988) sister to
27
Batesimalva was surprising, although the two share a chromosome number of n=16.
Bates (1968) originally placed Neobrittonia amongst the other pluriovulate genera of the
Abutilon alliance (e.g., Bakeridesia, Herissantia, Pseudabutilon, and Wissadula).
One of the other genera resolved as non-monophyletic in the ITS phylogeny was
Tetrasida. The genus currently contains four species (Fryxell and Fuertes Aguilar 1992)
found in Peru and Ecuador. Two species were included here to represent the genus: T.
chachapoyensis clusters with species of Wissadula, while T. weberbaueri is sister to
Allowissadula holosericea. Krapovickas (1969) included the species now considered as
Tetrasida in Abutilon section Tetrasida because he believed the condition of a fourmerous corolla (for which the genus was named) in the species was not sufficiently
consistent to merit generic recognition (Krapovickas 1969).
However, Fryxell and
Fuertes Aguilar (1992) resurrected the genus including two species and later (Fryxell and
Fuertes Aguilar 1997) described two new species.
The most basal clade of Clade A contains a medley of taxa: Hoheria, Sidasodes
colombiana, Sida hermaphrodita, and Sida hookeriana. The placement of Hoheria, a
genus endemic to New Zealand, outside the remaining members of the Malveae seems
logical in terms of its geographic distribution.
Very few genera in the tribe (only
members of the Plagianthus alliance and Howittia) are centered in the South Pacific
region. The placement of Hoheria with these “divergent” taxa is more likely a reflection
of the lack of sampling from Australia and New Zealand species, rather than a true
phylogenetic affinity. In fact, the inclusion of the remaining members of this alliance
provides additional resolution to the placement of this genus (Tate et al. in prep.). Also
included in this clade is Sida hookeriana (section Hookerianae), a species from
southwestern Australia (Fryxell 1985). The placement of this species near Hoheria is
intriguing and its basal position in the phylogeny could be evidence of South Pacific
28
origin for the tribe. However, two American species are also included in this clade:
Sidasodes colombiana (from the Andes of Colombia and Peru) and Sida hermaphrodita
(section Pseudo-Napaea) from the eastern United States. Fryxell and Fuertes Aguilar
(1992) noted the similarity of Sidasodes to Sida hermaphrodita on the basis of fruit
morphology. However, these taxa were not thought to share other features. Sidasodes
has a base chromosome number of x=5 which is common to members of the traditional
Sphaeralcea alliance, but the former is not closely related to any genera of the latter.
Instead, this species probably represents an aneuploid lineage of the clade.
Alliances and genera of Clade B
Clade B was resolved as a well-supported group (84% bootstrap support)
composed of genera from the Anisodontea, Kearnemalvastrum, Malva, Malvastrum,
Napaea, Phymosia, Sidalcea, and Sphaeralcea alliances (Figure 2.4). The clade contains
mostly American taxa, with the exception of Alcea (Mediterranean to central Asia),
Anisodontea (South Africa), Kitaibela (Europe), the Malva-Lavatera group (mainly north
temperate
areas),
(Mediterranean).
Navaea
phoenicia
(Canary
Island
endemic)
and
Malope
Again, several of the alliances (Malva, Phymosia, Sidalcea,
Sphaeralcea), as well as several genera (e.g., Iliamna, Malacothamnus, Tarasa) are not
monophyletic in the ITS phylogeny.
The three genera with multiverticillar carpels (Kitaibela, Malope, and Palaua)
that were once placed in a separate tribe Malopeae were all members of clade B (Figure
2.4). However, these three genera did not form a monophyletic group, which implies that
this unusual morphological feature has evolved on three separate occasions. Bates (1968)
placed Palaua in the Sphaeralcea alliance with the other x=5 genera, while retaining
Kitaibela and Malope as the sole members of the Malope alliance. He also proposed that
29
the evolution of the carpels into superposed verticils in Palaua occurred independently
from that of Malope and Kitaibela, an hypothesis which is supported here.
One important finding within clade B is the sister relationship of Urocarpidium
albiflorum to the Fuertesimalva species. Urocarpidium albiflorum, the type species of
the genus, was placed in synonymy with Tarasa operculata due to its apically plumose
awns on the mericarps (Fryxell 1996).
The genus Fuertesimalva was created to
accommodate the remaining species of Urocarpidium (Fryxell 1996). The results of the
ITS phylogeny (and also chloroplast data, see Chapter 3), do not support the separation of
U. albiflorum from the remaining species of Fuertesimalva nor its inclusion in Tarasa
operculata and argue for the original generic composition and name. In fact, calyx and
leaf vestiture seem to be a better indicator of phylogenetic relatedness for these species
than the presence of apical ornamentation on the mericarps. For example, in Tarasa the
calyx trichomes are stipitate stellate, whereas in Urocarpidium sensu lato, they are
hirsute. The two genera also differ in mericarp morphology: Urocarpidium mericarps
are indehiscent, glabrous, and typically laterally “ridged,” while Tarasa mericarps are
dehiscent (at least partially), have stellate pubescence on the dorsal and apical surfaces,
and the lateral walls may be smooth or reticulate. The occurrence of an apical awn on the
mericarps of these genera appears to be a convergent character and is found in other cases
as well (e.g., Tarasa species).
The sister relationship of a South American coastal genus (Palaua) to an Andean
genus (Fuertesimalva) is particularly interesting. Neither genus had been previously
suggested to be closely related to the other. Because the genera occurring in the high
Andes do not form a monophyletic clade (they are scattered within Clade A and Clade
B), multiple independent colonizations of the Andes by several Malveae genera are
inferred (e.g., Acaulimalva, Malvastrum, Nototriche, Sida, Sidasodes, and Tarasa).
30
Iliamna and Malacothamnus were both found to be not monophyletic (see
discussion of M. chilensis under Tarasa). Two species of Iliamna (I. bakeri and I.
lactebracteata) (x=33) which are endemic to northern California/southern Oregon are
more closely related to Sidalcea (x=5) and Eremalche (x=5) which are distributed along
the western coast of North America than to the remaining members of Iliamna. The other
two species included here (I. rivularis, found in the Rocky Mountains of the United
States and I. remota, found in Illinois, Indiana, and Virginia), cluster with Phymosia
(Mexico and Caribbean) (x=17) and Malacothamnus fasciculatus (California) (x=17).
Iliamna, Malacothamnus, Neobrittonia and Phymosia were grouped together in the
Phymosia alliance by Fryxell (1988). The Iliamna/Malacothamnus/Phymosia group is
currently being studied in greater detail (Slotta pers. comm.).
Among the non-monophyletic genera was Tarasa (x=5), a high elevation Andean
genus, which ranges from central Peru to southern Argentina and adjacent Chile, with 2
species disjunct in central Mexico (Krapovickas 1954).
In the ITS phylogeny,
Malacothamnus chilensis was placed sister to Tarasa machupicchensis and T. mandonii
of Peru and Bolivia, respectively (a finding also corroborated by chloroplast data, see
Chapter 3). Malacothmanus chilensis (x=6) is the only South American species (southern
Chile) of a genus otherwise composed of x=17 North American species (Krapovickas
1952, Bates 1968, Bates and Blanchard 1970). Recent chromosome counts made during
a systematic study of Tarasa revealed that T. machupicchensis and T. mandonii share this
chromosome number of x=6 (see Chapter 3). Morphologically, these species also share a
shrubby habit and relatively large flowers. Based on the unusual shared chromosome
number, morphological evidence, and molecular phylogenetic results, these taxa merit
recognition at the generic level (Tate in prep.).
31
Even with the removal of the x=6 species from Tarasa, the genus is still not
monophyletic. Sphaeralcea (x=5), a North-South American temperate disjunct genus, is
sister to a clade containing the remaining species of Tarasa and the ultra-high elevation
(> 4000 m) Andean genus Nototriche (x=5). These three genera had been suggested to be
close relatives based on gross morphology and mitotic chromosome morphology
(Krapovickas 1960). The two North American Sphaeralcea species sampled cluster
together, as do the two South American taxa included. The species of Nototriche are
well-embedded within the Tarasa clade. Further sampling of these two genera is needed
to resolve the relationship among the species.
CONCLUSIONS
Results from this study, like several previous phylogenetic works, suggest that the
infratribal systematics and even generic boundaries of the Malveae need to be revised.
Before formal proposals are made, however, the phylogenetic relationships recovered in
the nuclear-based phylogeny should be compared to a chloroplast-based phylogeny. True
phylogenetic
relationships
may
be
obscured
in
situations
where
polyploidy
(allopolyploidy) and/or hybridization commonly occur (Doyle 1992, Wendel and Doyle
1998). The former at least appears to be a common speciation mechanism in many
lineages of the Malveae. The extent of hybridization between lineages within the tribe
remains untested to date.
Future work which utilizes both nuclear and chloroplast
molecular data and which focuses on the systematics of closely related genera should
shed light on this issue.
32
Chapter 3: Molecular systematics of the polyploid Andean genus
Tarasa Philippi (Malvaceae)
ABSTRACT
A molecular phylogenetic analysis of the Andean genus Tarasa (Malvaceae) and
related genera yielded unexpected results regarding generic boundaries, the origins of
polyploidy, and the morphological attributes of the polyploid taxa.
As currently
circumscribed, the 30 species of Tarasa (diploid and tetraploid) are morphologically
characterized by their magenta, lilac, or white flowers displayed in axillary scorpioid
cymes, apically aristate, completely dehiscent mericarps, and a base chromosome number
of x=5. The species are found across a range of elevations (800-4200 m) from central
Peru to southern Chile and adjacent Argentina, with two species disjunct in central
Mexico. The tetraploid species occupy higher elevation habitats in the Andes than the
diploids. Further, the tetraploids are unusual in that they are all annuals and have reduced
floral morphologies (smaller flowers and fewer anthers) compared to the diploid species.
Nuclear (ITS) and chloroplast (psbA-trnH and trnT-trnL spacers, matK-3’trnK intron)
sequence data were used to reconstruct independent phylogenies to test monophyly of the
genus, determine its sister group(s), and investigate the origin of the polyploid species.
Neither the nuclear nor the chloroplast phylogeny supports monophyly of Tarasa as
currently circumscribed.
The high Andean genus Nototriche and the North/South
American disjunct genus Sphaeralcea are placed within the Tarasa clade. Also, a clade
consisting of the shrubby species Tarasa machupicchensis, T. mandonii, and T.
spiciformis plus Malacothamnus chilensis (n=6) was placed outside of and sister to the
remaining species of Tarasa and included genera. Chromosome counts have verified that
these Tarasa species are n=6 and should not be considered part of Tarasa. Lastly, the
33
polyploid species of Tarasa do not form a monophyletic clade and thus have been
generated multiple times. These findings suggest that the shared morphological features
of the tetraploids are the result of convergent evolution and not shared ancestry.
INTRODUCTION
Taxonomic history
The genus Tarasa was first described by Philippi in 1891 for a single species,
Tarasa rahmeri Philippi, from Tarapacá, Chile. The genus (locally called ‘Tarasa’) was
primarily distinguished from other mallows by its completely dehiscent and apically
aristate mericarps. Philippi (1891) contrasted the genus with Cristaria with which it
shares apical mericarp ornamentation, but in Cristaria these appendages are broad and
wing-like. A second species, Tarasa albertii Philippi [as Tarassa alberti Philippi], from
the Andes of southern Argentina was added in 1893. In 1949, Kearney transferred
Sphaeralcea mandonii and Malva plumosa to Tarasa bringing the total number of species
to four. During the interim between Philippi’s descriptions and Kearney’s publication
and a few years thereafter, the remaining species now considered Tarasa were variously
described as species of Malva, Malvastrum, or Sphaeralcea. In a 1954 synopsis of
Tarasa, Krapovickas expanded the genus to include 24 species by proposing 13 new
combinations and describing 8 new species. Since then, six additional species have been
included in the genus (Bates 1965, Krapovickas 1960, 1965, 1970, 1979).
As
traditionally circumscribed, the species of Tarasa are characterized by axillary scorpioid
cymes bearing lilac, magenta, or white flowers (except solitary in Tarasa humilis and
umbellate in Tarasa umbellata and Tarasa reichei), apically aristate and completely
dehiscent mericarps, and a base chromosome number of x=5 (Krapovickas 1954).
Krapovickas (1960) created two sections within Tarasa (Table 3.1): section Umbellatae
34
containing the two species (T. umbellata and T. reichei) in southern Chile that have
umbels and exserted anthers and stigmas, and section Tarasa composed of the remaining
species. The latter was further divided into three informal groups that differed in habit,
leaf morphology, and ploidy level.
Chromosomes, geography, and morphology
As part of his revision of the genus, Krapovickas (1954, 1960) conducted
cytological studies which revealed the presence of both diploids (2n=10) and tetraploids
(2n=20). These counts, combined with those made in this study total nineteen of the
thirty species: nine of these are 2n=10, two are 2n=12, and eight are 2n=20 (Table 3.1).
Polyploidy is particularly interesting in Tarasa because characters of the species violate
two axioms of polyploid groups. First, polyploids are typically perennials (Stebbins
1940, 1950), however, the polyploid species of Tarasa are all annuals (Krapovickas
1954, 1960). Second, because polyploidization leads to increased cell size, the gross
morphological features of polyploids described to date are larger than their diploid
relatives (Gustafsson 1948, Stebbins 1940, 1950). Yet, the tetraploid species of Tarasa
have an overall reduced morphology which includes smaller flowers with fewer anthers
than the diploid species (Krapovickas 1960, 1971). In a palynological study, Tressens
(1970) found the pollen of several tetraploid Tarasa species (range = 25-27 µm in
diameter) to be generally smaller than that of most diploid pollen (range = 27.5-32.0 µm
in diameter). Tetraploid pollen grains were found to have more apertures than diploid
pollen grains (6-10 versus 3-5) and were ovoid in shape (versus spherical in diploid
species) (Tressens 1970).
35
Table 3.1
Tarasa as circumscribed (Krapovickas 1954, 1960, 1971; Bates 1965) and
chromosome numbers. Ploidy level was inferred for species without
previously published chromosome counts – see page 50 for details.
2n
2n
published inferred
Circumscription of Tarasa
Chromosome
count reference
SECTION TARASA
DIPLOID PERENNIALS
T. albertii Phil.
T. capitata (Cav.) Bates
T. humilis (Gillies ex Hook. et Arn.) Krapov.
T. machupicchensis Krapov.
T. mandonii (Baker) Kearney
T. operculata (Cav.) Krapov.
T. rhombifolia Krapov.
T. spiciformis Krapov.
T. thyrsoidea Krapov.
10
10
10
12
12
10
Krapovickas 1949
Krapovickas 1960
Krapovickas 1960
This chapter
This chapter
Krapovickas 1960
10
12
10
This chapter
10
10
Krapovickas 1954
Fernandez 1974
DIPLOID ANNUALS
T. cardenasii Krapov.
T. heterophylla (Gris.) Krapov.
T. latearistata Krapov.
T. meyeri Krapov.
T. trisecta (Gris.) Krapov.
10
10
10
Krapovickas 1954
Krapovickas 1960
20
20
Krapovickas 1960
Krapovickas 1960
TETRAPLOID ANNUALS
T. antofagastana (Phil.) Krapov.
T. cerrateae Krapov.
T. congestiflora (Johnston) Krapov.
T. corrugata Krapov.
T. geranioides (Cham. et Schl.) Krapov.
T. marinii Krapov.
T. martiniana Krapov.
T. nototrichoides Krapov.
T. odonellii Krapov.
T. pediculata Krapov.
T. tarapacana (Phil.) Krapov.
T. tenella (Cav.) Krapov.
T. tenuis Krapov.
T. urbaniana (Ulbr.) Krapov.
SECTION UMBELLATAE
T. reichei (Phil.) Krapov.
T. umbellata Krapov.
20
20
20
This chapter
20
20
20
20
Krapovickas 1954
20
20
20
20
20
Krapovickas 1960
Krapovickas 1960
Krapovickas 1954
Krapovickas 1960
10
10
36
In addition to these anomalous morphological features, the species of Tarasa
exhibit a distinct relationship between ploidy level and geographic distribution (Figure
3.1). The diploid species, many of which have restricted distributions, occur at midelevations (between 800-3500 m) and form a discontinuous belt along both sides of the
Andean Cordillera (Krapovickas 1960, 1971). The tetraploids occupy the interior of the
diploid ring at higher elevations (2500-4200 m) and are usually weeds in disturbed or
cultivated habitats of the central Andean altiplano (Krapovickas 1954, 1960, 1971).
Areas historically subjected to disturbance typically have comparatively high occurrences
of polyploidy, especially those affected by the advance and retreat of Pleistocene ice
sheets (Stebbins 1950, Ehrendorfer 1980).
Krapovickas (1971) suggested that the
morphological plasticity and greater stress tolerance of the tetraploids allowed them to
occupy new areas at higher altitudes that were previously not occupied by the genus. An
origin for the polyploid species was not suggested nor whether they were auto- or
allopolyploid derivatives (Krapovickas 1960, 1971).
Systematic position
Tarasa has been allied to other x=5 genera belonging to the Sphaeralcea alliance
in the tribe Malveae. In particular, the genera Sphaeralcea A. St.-Hil., Nototriche Turcz.
and Acaulimalva (Cav.) Krapov. were suggested to be close relatives (Krapovickas 1960,
1971). Sphaeralcea (40 species) occurs in temperate North and South America and has
similar mericarp morphology to Tarasa (Krapovickas 1960, 1971).
Nototriche is a
distinctive Andean (Ecuador to Argentina) clade of approximately 100 perennial (rarely
annual) acaulescent species usually found at altitudes above 4000 m (Fryxell 1997). A
few tetraploid species of Tarasa share with Nototriche the unusual morphological
character of a sheath formed by fused stipules, petiole, and peduncle (Krapovickas 1960).
37
Figure 3.1
Distribution of Tarasa in the Americas. The map was generated using the
program DIVA-GIS (Hijmans et. al 2002). Localities are based on label
information from approximately 1400 herbarium specimens. Diploid
(2n=10) species are indicated by red dots; tetraploid (2n=20) species are
indicated by yellow dots.
38
Acaulimalva is also an Andean genus (19 species) of acaulescent perennial herbs
distributed at high elevations (2900-4800 m) from Venezuela and Colombia to Bolivia
and northwestern Argentina (Krapovickas 1974, Fryxell 1997). Tarasa humilis, the only
species in the genus with an acaulescent habit and solitary flowers, was suggested to be a
link between either the perennial species of Nototriche or the genus Acaulimalva
(Krapovickas 1960).
In particular, Krapovickas (1971) noted that Sphaeralcea,
Nototriche, and Tarasa have mitotic chromosomes of similar size and morphology which
could indicate a close relationship among them. Urocarpidium albiflorum Ulbr., the type
species of that genus, was placed in synonymy with Tarasa operculata due to the
presence of an apically plumose awn on the mericarps (Fryxell 1996).
The genus
Fuertesimalva Fryxell was created to accommodate the remaining species of
Urocarpidium (Fryxell 1996).
Krapovickas (1949) earlier in a footnote had also
suggested that Urocarpidium could be synonymous with Tarasa since both were
separated from Malvastrum on the basis of apically aristate fruits (Ulbrich 1917).
However, it is not clear from his remarks whether he was able personally to examine any
specimens of Urocarpidium.
Previous molecular-based phylogenies provided preliminary evidence for the
systematic position of Tarasa. An earlier chloroplast DNA restriction site analysis of the
Malvaceae s.s. placed Sphaeralcea and Tarasa as sister taxa (La Duke and Doebley
1995). However, neither Nototriche nor Acaulimalva were included in this study. Our
recent and more inclusive ITS phylogeny of the tribe Malveae revealed surprising and
complex relationships of Tarasa to its allies (Figures 2.1 and 2.4, previous chapter.).
Unexpectedly, the genus was found not to be monophyletic due to the inclusion, in a
single well-supported clade with Tarasa, of Malacothamnus chilensis (Gay) Krapov. and
all species of Nototriche and Sphaeralcea sampled. Acaulimalva was not closely related
39
to this group, despite its similar geographic distribution, habit, and chromosome number.
The placement of Malacothamnus chilensis sister to Tarasa machupicchensis, T.
mandonii, and T. spiciformis was not anticipated, but is logical given its geographic
distribution, shared chromosome number of n=6 (M. chilensis, Bates and Blanchard
1970; T. mandonii, A. Krapovickas pers. comm. and Table 3.1, T. machupicchensis,
Table 3.1) and overall similar morphology to those Tarasa species. The remaining
species of Malacothamnus have a chromosome number of n=17 and are confined to the
western coast of North America from California to Baja California (Fryxell 1997). The
species of Sphaeralcea sampled (both North and South American representatives) formed
a monophyletic clade sister to the remaining species of Tarasa and Nototriche in that ITS
phylogeny.
The goals of this study were three-fold to: 1) assess further the monophyly of
Tarasa as currently circumscribed using both nuclear and chloroplast sequence data, 2)
determine the sister taxon to Tarasa, and 3) unravel the origins of the polyploid species
of Tarasa. In this chapter, I examine in more detail the phylogenetic relationships within
Tarasa and to the genera Nototriche and Sphaeralcea.
Evidence is drawn from
morphological and cytological data as well as nuclear and chloroplast-based phylogenies.
MATERIALS AND METHODS
Taxon sampling
Outgroup choice was initially based on the suggested close relationship of Tarasa
to Acaulimalva, Nototriche, and Sphaeralcea (Krapovickas 1960, 1971). Only the most
closely related genera as determined from a larger ITS phylogeny of the tribe Malveae
have been included here (see previous chapter). Twenty-seven of the thirty species of
40
Tarasa were included and when possible multiple populations of wide-ranging taxa were
sampled (Table 3.2).
DNA extraction and amplification
Plant (leaf) material used in the molecular analyses was collected in the field and
dried in silica gel (Chase and Hills 1991) or sampled from herbarium specimens.
Alternatively, seeds removed from herbarium specimens or collected in the field were
grown in the greenhouse and fresh leaf material from them was used. Total genomic
DNA was extracted by the “rainforest” method of Scott and Playford (1996) or the
modified CTAB protocol (Doyle and Doyle 1987) of Loockerman and Jansen (1996).
The addition of a phenol extraction aided in obtaining clean DNA from recalcitrant
species (especially from older herbarium specimens). Vouchered DNAs of two species
were generously provided by Mark Chase at Kew Gardens (Tarasa humilis) and John La
Duke at The University of North Dakota (Tarasa cerrateae). Voucher specimens and
their locations are indicated in Table 3.2.
Three non-coding regions were used to reconstruct a chloroplast-based phylogeny
of the combined
psbA-trnH spacer, trnT-trnL spacer, and the 3’ intergenic region
between matK and trnK. Primer sequences and their references are listed in Table 3.3.
Two primers (trnH2 and matK-M) were modified from previously published primers
specifically for this project and were based on Gossypium and Tilia sequences for these
regions, respectively. The program Primer3 (Rozen and Skaletsky 1997) aided in primer
design. Because the trnT-trnL region was approximately 1400 bp in length, it was
difficult to amplify for some taxa (especially those extracted from herbarium specimens)
and to sequence reliably the entire fragment. Thus, an internal primer (Table 3.3) was
41
Table 3.2
Vouchers used in molecular analysis, their location and GenBank accession number. An asterisk indicates
voucher for chromosome count. A letter designates separate populations sampled.
Species
Source/Voucher
Locality
Fuertesimalva jacens (S. Watson) P.A. Fryxell
Fuertesimalva limensis (L.) P. A. Fryxell
Malacothamnus chilensis (Gay) Krapov.
Modiola caroliniana G. Don
Modiolastrum lateritium (Hook.) Krapov.
Monteiroa bullata (Ekman) Krapov.
Nototriche anthemidifolia A. W. Hill
Nototriche flabellata A. W. Hill
Nototriche pedicularifolia (Meyen) A. W. Hill
Sphaeralcea angustifolia G. Don
Sphaeralcea cordobensis Krapov.
Sphaeralcea crispa Hook.
Sphaeralcea philippiana Krapov.
Sphaeralcea wrightii A. Gray
Tarasa albertii Phil. A
Tarasa albertii Phil. B
Koch and Fryxell 77326 (LL)
Tate and Timana 60 (TEX)
Meudt et al. HM-028 (TEX)
Tate and Simmons 50 (TEX)
Tressens et al. 23-XI-93 (TEX)
Krapovickas and Cristobal 43703 (TEX)
Tate et al. 85 (TEX)
Funk and Bernal H. 11289 (TEX)
Tate et al. 102 (TEX)
Tate et al. 51 (TEX)
Tate et al. 79 (TEX)
Tate et al. 77 (TEX)
Tate et al. 71 (TEX)
Tate et al. 53 (TEX)
Böcher et al. 978 (MO)
Tate et al. 74 (TEX)
MEXICO - Mexico - Tenango de Arista
PERU - Ancash - Yungay
CHILE - Santiago
MEXICO - Puebla
ARGENTINA - Misiones - Guaraní
BRASIL - Santa Catarina
ARGENTINA - Salta - Santa Victoria
BOLIVIA - La Paz - Murillo
PERU - Arequipa - Arequipa
MEXICO - San Luis Potosi
ARGENTINA - Cordoba
ARGENTINA - La Pampa - Caleu Caleu
ARGENTINA - Mendoza - Las Heras
MEXICO - Tamaulipas
ARGENTINA - Mendoza - Atuel Valley
ARGENTINA - Mendoza - Malargüe
42
Table 3.2
continued
Species
Tarasa antofagastana (Phil.) Krapov. A
Tarasa antofagastana (Phil.) Krapov. B
Tarasa antofagastana (Phil.) Krapov. C
Tarasa capitata (Cav.) Bates A
Tarasa capitata (Cav.) Bates B
Tarasa cardenasii Krapov.
Tarasa cerrateae Krapov.
Tarasa congestiflora (Johnston) Krapov.
Tarasa corrugata Krapov.
Tarasa geranioides (Cham. et Schl.) Krapov.
Tarasa heterophylla (Gris.) Krapov.
Tarasa humilis (Gillies ex Hook. et Arn.) Krapov. A
Tarasa humilis (Gillies ex Hook. et Arn.) Krapov. B
Tarasa latearistata Krapov.
Tarasa machupicchensis Krapov.
Tarasa mandonii (Baker) Kearney A
Tarasa mandonii (Baker) Kearney B
Tarasa marinii Krapov.
Source/Voucher
Krapovickas and Cristobal 46656
(TEX)
Tate et al. 92 (TEX)
Tate et al. 66 (TEX)
Tate and Timana 63 (TEX)
Candia 78 (LPB)
Nee 44773 (TEX)
Treacy 794 (NY)
Ricardi et al. 189A (CONC)
Tate and Symonds 110 (TEX)
Rzedowski 31482 (NY) *
Krapovickas et al. 18830 (LL)
Chase 3965 (K)
Tate et al. 69 (TEX)
Nuñez 637 (MCNS)
Timana and Tate 3771 (TEX) *
Fortunato et al. 1893 (TEX)
Beck and Seidel 14510 (TEX) *
Tupayachi 986 (MO)
43
Locality
ARGENTINA - Jujuy - Tumbaya
ARGENTINA - Tucumán - Tafí
MEXICO - Veracruz
PERU - Arequipa - Arequipa
BOLIVIA - Cochabamba - Quillacollo
BOLIVIA – Santa Cruz - Vallegrande
PERU - Arequipa - Coporaque
CHILE - I Reg. de Tarapaca - Arica
PERU - Cuzco - Calca
MEXICO - Hidalgo - Tepeapulco
BOLIVIA - Tarija - Tarija
ENGLAND - Kew Gardens
CHILE - IX Reg. de Araucania
ARGENTINA - Salta - Cachi
PERU - Cuzco - Urubamba
BOLIVIA- La Paz
BOLIVIA - Cochabamba - Ayopaya
PERU - Cuzco - Urubamba
Table 3.2
continued
Species
Tarasa meyeri Krapov.
Tarasa nototrichoides Krapov.
Tarasa odonellii Krapov.
Tarasa operculata (Cav.) Krapov. A
Tarasa operculata (Cav.) Krapov. B
Tarasa pediculata Krapov.
Tarasa rhombifolia Krapov.
Tarasa spiciformis Krapov.
Tarasa tarapacana (Phil.) Krapov.
Tarasa tenella (Cav.) Krapov. A
Tarasa tenella (Cav.) Krapov. B
Tarasa tenuis Krapov.
Tarasa thyrsoidea Krapov. A
Tarasa thyrsoidea Krapov. B
Tarasa trisecta (Gris.) Krapov.
Tarasa urbaniana (Ulbr.) Krapov.
Urocarpidium albiflorum Ulbr.
Source/Voucher
Krapovickas et al. 22022 (F)
Menhofer X-1093 (LPB) *
Zamora and Quiroga 19 (LPB)
Dillon et al. 4790 (TEX)
Tate and Timana 62 (TEX)
Baumann 234 (CONC)
Tate and Symonds 109 (TEX)
Weberbauer 7512 (F)
Krapovickas and Shinini 35752 (CTES)
Krapovickas 47722 (TEX)
Solomon and Moraes 13401 (MO)
Aguilar s.n. (TEX)
Dillon et al. 3243 (TEX)
Tate et al. 107 (TEX)
Tate et al. 91 (TEX)
Leiva and Leiva 488 (F)
Burandt 2340 (F)
44
Locality
ARGENTINA - Salta - Cachi
BOLIVIA - La Paz - F. Tamayo
BOLIVIA - La Paz - Murillo
PERU- Moquegua - Mariscal Nieto
PERU - Arequipa - Arequipa
CHILE – II Region – El Loa
PERU - Cuzco - Urubamba
PERU - Ayacucho - Huanta
ARGENTINA - Jujuy - Humahuaca
ARGENTINA - Jujuy - Humahuaca
BOLIVIA - La Paz - Murillo Province
PERU - Puno - Amantani
PERU - Arequipa - Caraveli
PERU - Arequipa - Caraveli
ARGENTINA - Salta - Cafayate
PERU - La Libertad - Otuzco
PERU - Moquegua
Table 3.3
List of primers used for PCR amplification and sequencing
Region
Primer
name
ITS
7A
ITS
1A
ITS (1)
2b
ITS (2)
3
ITS
4
trnT-trnL spacer
A
trnT-trnL spacer
B
trnT-trnL spacer
IF
trnT-trnL spacer
IR
psbA-trnH spacer
psbA
psbA-trnH spacer
trnH2
matK-3' trnK intron matKM
matK-3' trnK intron trnK-2R
Primer sequence (5'-3')
Reference
GAGTCATCAGCTCGCGTTGACTA
GGAAGGAGAAGTCGTAACAAGG
CTC GAT GGA ACA CGG GAT TCT GC
GCA TCG ATG AAG AAC GCA GC
TCC TCC GCT TAT TGA TAT GC
CAT TAC AAA TGC GAT GCT CT
TCT ACC GAT TTC GCC ATA TC
GGA ATA CTC GAA CGG
CCG TTC GAG TAT TCC
GTT ATG CAT GAA CGT AAT GCT C
CGC GCA TGG TGG ATT CAC AAT CC
TCGACTTTCTGGGCTATC
AACTAGTCGGATGGAGTAG
A. Plovanovich and J. Panero unpublished
Downie and Katz-Downie 1996
Based on Kim and Jansen 1994
Kim and Jansen 1994
White et al. 1990
Taberlet 1991
Taberlet 1991
This publication
This publication
Sang et al. 1997
This publication
This publication
Johnson and Soltis 1994
45
designed in a conserved region approximately 1000 bp from the 5’ end in order to
amplify and sequence this region better. Likewise, some taxa were cloned (TOPO TA
cloning kit, Invitrogen) for this same region when insufficient polymerase chain reaction
(PCR) product was obtained for sequencing. Each chloroplast region was PCR amplified
in a 50 uL cocktail consisting of 1x MasterAmp Tfl PCR buffer (Epicentre), 2.5 µM
MgCl2, 0.8 µM DNTPs, 0.05 µM DMSO, 0.5 unit TFL polymerase (Epicentre) and 0.4
µM forward and reverse primers. After an intitial denaturing step at 95° for 5 minutes
(“hot start”), the reactions were held at 72° while polymerase was added to each reaction.
Amplification of each chloroplast region proceeded at 94° for 3 min, 46-52° for 1 min.,
72° for 1 min., followed by 35 cycles of 94° 1 minute, 46-52° 1 minute, 72° for 45 sec. +
3 sec/cyc. with a final 7-15 minute extension at 72°. Amplification products were
separated on a 1% agarose gel, stained with ethidium bromide and visualized with UV on
a transilluminator. PCR products were cleaned using Qiagen QIAquick spin columns
following manufacturer’s instructions. Cycle sequencing was performed using Big Dye
terminator chemistry and either the forward or reverse primer. Centri-sep columns were
used to remove residual salts and unincorporated nucleotides from the sequencing
products. Automated sequencing was performed on an ABI 3700 at the DNA Analysis
Laboratory at The University of Texas at Austin.
The internal transcribed spacer (ITS) region of the 18S-25S nuclear ribosomal
repeat was chosen for nuclear phylogeny reconstruction. Amplification and sequencing
of the ITS region was conducted as for the chloroplast regions. Amplification primers
used were 7A (A. Plovanovich and J. Panero unpublished) and 4 (White et al. 1990) or
for ITS 1 and ITS 2 separately, primers 7A and 2b (Kim and Jansen 1994) and primers 3
(Kim and Jansen 1994) and 4, respectively. Primer sequences are given in Table 3.3.
The ITS region was cloned (TOPO TA cloning kit, Invitrogen) for individuals in which
46
the directly sequenced PCR product contained polymorphic sites, i.e., where two
discernible peaks appeared in the chromatogram. Colonies were screened for inserts
using PCR and among these, 5-10 clones per reaction were selected for sequencing.
Sequence analysis
The boundaries of each target sequence were determined by comparison to
published Gossypium (ITS, psbA-trnH, trnT-trnL) or Tilia (matK-3’trnK) sequences.
BLAST (Altschul et al. 1990) searches in GenBank were conducted to detect potential
contaminant sequences. Where multiple populations or accessions of the same taxon had
identical sequences (for ITS or chloroplast regions), only one sequence was used in the
analyses.
Complementary strands were edited using Sequencher (Gene Codes
Corporation 1995). The sequences were aligned using Clustal X (Thompson et al. 1997)
with manual adjustments as needed. Sequence length and GC content were calculated in
MacClade 4.0 (Maddison and Maddison 2000). Pairwise divergence values and base
composition (biased) were assessed using PAUP* (Swofford 2002).
Although the ITS spacers are not protein coding regions, they are still constrained
to maintain a particular secondary structure for proper processing (Baldwin et al. 1995).
Nucleotide substitutions or insertion-deletion events which may alter the expected
secondary structure can be used as evidence of non-functional copies (Buckler and
Holtsford 1996, Mayol and Rosello 2001). Likewise, an excess of observed nucleotide
substitutions (particularly in conserved regions) in copies would indicate that nuclear
repeats exist which are not under selection (Li 1983, Mayol and Rosello 2001).
Therefore, cloned ITS sequences were examined in an attempt to identify non-functional
or divergent paralogues. Sequences were evaluated for angiosperm-conserved regions
within ITS1, 5.8S, and ITS2 (Liu and Schardl 1994, Hershkovitz and Zimmer 1996,
47
Mayol and Rossello 2001).
If cloned sequences contained deviant nucleotide
substitutions in any of these conserved areas and/or if they contained an excess of
nucleotide substitutions overall (relative to the remainder of the data set and/or as
compared to the directly sequenced product), then they were considered to be paralogous
copies and were discarded from further analyses.
The program GeneConv (Sawyer 1999) was used to test for potential
recombination of ITS sequences between taxa (Smith 1999, Posada and Crandall 2001).
The cloned ITS data set was examined using the default settings (returning only
significant global fragments).
Phylogenetic reconstruction
Two data sets (ITS and all chloroplast regions combined) were analyzed
separately under the parsimony optimality criterion of PAUP* v. 4.0b10 (Swofford
2002).
The Independent Length Difference Test (ILD) of Farris (1994), which is
implemented in PAUP* as the Partition Homogeneity Test, was first conducted to test for
homogeneity among data partitions. For the chloroplast data set, three partitions were
defined: matK-3’trnK intron, psbA-trnH spacer, and trnT-trnL spacer. A second test
compared each chloroplast region (pairwise). Finally, a test combining ITS and all
chloroplast regions examined two partitions (ITS and all chloroplast regions combined).
Because different numbers of sequences were available for the two data sets, the number
of ITS clones included were trimmed to mirror the species relationships recovered in
initial analyses of the chloroplast phylogeny (thus creating a molecular phylogeny
representative of a maternal lineage). For all ILD tests, 100 replications were performed.
Heuristic searches were employed with TBR branch swapping, ACCTRAN character
optimization and gaps coded as missing.
48
Additional tests were conducted in PAUP* to assess the apparent incongruence of
the chloroplast and ITS phylogenies (reviewed in Mason-Gamer and Kellogg 1996 and
Johnson and Soltis 1998). The compare-2, Templeton, and Kishino-Hasegawa tests were
conducted to test the null hypothesis that the data sets are congruent and that the observed
differences in the phylogenies are due to chance. For all three tests, the strict consensus
tree (either ITS or chloroplast) was designated as the constraint for the rival data set.
Each data set was tested for hierarchical structure by evaluating 10,000 randomly
generated trees and comparing the calculated g1 to critical values (Hillis and
Huelsenbeck 1992). Modiola and Modiolastrum were the designated outgroups in each
analysis.
For phylogeny reconstruction under parsimony, an heuristic search was
employed with 1000 random addition replicates, TBR branch swapping, ACCTRAN
character-state optimization and gaps coded as missing (ITS) or recoded as binary
characters (combined chloroplast data matrix). Branch lengths of zero were collapsed.
Support for monophyly of the clades was determined by 100 bootstrap replications
(Felsenstein 1985).
The likelihood ratio test (Goldman 1993, Yang 1996, Huelsenbeck and Rannala
1997) was conducted to determine the model of evolution that best describes each data
set. Likelihood scores were calculated for the most parsimonious trees generated from an
heuristic search (1000 RAR, TBR branch swapping, ACCTRAN optimization in effect,
gaps treated as missing) in PAUP* for five increasingly complex models: Jukes-Cantor,
HKY-85, GTR, GTR+G, and GTR+G+I . Pairwise values [(more complex model - less
complex model)*2] were compared to a Chi square distribution.
The best model
(GTR+G+I) as determined by the likelihood ratio test was then used in the constraint
analyses and simulations.
49
Initital phylogenetic analyses resulted in unexpected relationships. In order to test
alternative hypotheses of evolution in Tarasa, parametric bootstrapping (Hillis et al.
1996) was also conducted as follows: 1) all traditionally circumscribed species of Tarasa
were constrained to monophyly, 2) as 1, except excluding T. machupicchensis, T.
mandonii, and T. spiciformis (n=6 group), and 3) all known and suspected Tarasa
tetraploids constrained to monophyly. Chromosome number was inferred for species of
Tarasa lacking direct counts based on habit, geographic distribution, and floral
morphology. All perennials were considered diploid (2n=10) (excluding the n=6 group).
Annuals with a restricted geographic range, petal length greater than 3 mm, and anthers
numbering more than 30 were considered diploid (2n=10).
Annuals with a wide
geographic range, petal length less than 3 mm, and anthers fewer than 20 were considered
tetraploid (2n=20). The single “best” constraint tree was then used as the model tree in
SeqGen (Rambaut and Grassly 1997). Zero branch lengths were added to the model tree
(before simulations in SeqGen) using the “Resolve tree” function in TreeEdit (Rambaut
and Charleston 2001). One hundred data sets were simulated in SeqGen (Rambaut and
Grassly 1997) using the model constraint tree and likelihood parameters. Each dataset
was then analyzed in PAUP* (with and without the appropriate constraint) employing
heuristic searches with 500 random addition sequences, TBR branch swapping, and gaps
coded as missing. Tree scores with and without the constraint were compared to the best
empirical unconstrained and constrained phylogenies.
Chromosome counts
Material used to conduct chromosome counts included personal collections, plants
grown from seeds harvested from herbarium specimens, or gifts (Krapovickas).
Permission was obtained from herbaria for judicious removal of seeds from specimens.
50
Seeds were considered mature if "full" and "healthy looking" (not collapsed). Seeds were
soaked in a 10% bleach solution for 10 minutes followed by 10 minutes in sterile water.
After soaking, the seed coats were nicked with a clean razor blade. For each species 3-4
seeds were placed in a petri dish with sterile water. The petri dish was wrapped with
parafilm, and placed in a lighted chamber (85° day/70° night) until germination. Seeds
from herbarium specimens up to 25 years old germinated and produced vigorous
individuals. When seedlings had completely emerged from the seed coats, they were
transplanted to a 2 1/2" plastic pot with a 3:1:1 mix of Scott's Metro Mix 500:fine
sand:coarse granite. The soil was thoroughly moistened and the pots placed in a domed
flat in the same lighted chamber. After the second set of true leaves emerged, the plants
were weaned from the high humidity environment by gradually exposing them to open
air. After several weeks in the 2 1/2" pots, the plants were transplanted to 4" clay pots.
Plants were watered daily to every other day and were given a weekly treatment of
commercial fertilizer.
A young inflorescence was removed from an individual plant and placed in 3:1
(absolute ethanol:glacial acetic acid) for 24 hours. The buds were then transferred to
70% ethanol and kept at 4° C until counts were conducted. A range of buds was
examined for the correct stage of meiosis. The anther mass was teased out of the buds
and placed on a clean microscope slide. A drop of the DNA-binding flourochrome DAPI
(4’, 6-diamidino-2-phenylindole) was placed on the slide and the anther mass was
macerated with the blunt end of a glass rod for 2-3 minutes. A cover slip was placed on
top and pressure was applied to spread out the chromosomes. Under UV on an Olympus
BX60 light microscope, a rapid low power scan (at 200x) was first conducted to ensure
that the cells were at the correct stage. Digital images were captured with an Optronics
Magnafire S60800 at high power (1000x).
51
RESULTS
New chromosome counts
Haploid chromosome counts were made for four species of Tarasa and are
included in Table 3.1: Tarasa thyrsoidea n=5, T. geranioides n=10, T. machupicchensis
n=6, T. mandonii n=6.
Chloroplast sequence characteristics
Sequences of the three chloroplast regions (matK-3’trnK, psbA-trnH, trnT-trnL)
were obtained for 27 of the 30 species of Tarasa (multiple populations sampled for 8
species), 5 species of Sphaeralcea, 3 species of Nototriche, 2 species of Fuertesimalva,
and 1 species each of Urocarpidium, Malacothamnus, Modiola, and Modiolastrum (53
sequences in total), except that only a portion of the trnT-trnL intergenic spacer was
sequenced for two species of Tarasa (T. cardenasii and T. congestiflora) and one species
of Nototriche (N. flabellata) (Appendix B).
These sequences will be deposited in
GenBank prior to publication. Sequence statistics for each region [only for species of
Tarasa] are given in Table 3.4. The matK-3’trnK region sequenced for species of Tarasa
included approximately 774 base pairs (509 bp of the matK exon and 265 bp of the
intron). This region had the lowest pairwise divergence values (0-2.2% within Tarasa)
but the greatest mean GC content (33.3%) of the three chloroplast regions sequenced.
Variation within the matK-3’trnK region consisted entirely of nucleotide substitutions.
The trnT-trnL intergenic spacer was the longest of the three regions sequenced, varying
from 1261 to 1500 bp. This region had pairwise divergence values ranging from 0-4.4%
(within Tarasa) and a mean GC content of 21.1%. Both nucleotide substitutions and
insertion-deletion events were sources of variation within the trnT-trnL region. A total of
52
Table 3.4
Sequence statistics for species of Tarasa as calculated in MacClade (length
and GC content) and PAUP* (pairwise divergence).
Region
ITS 1
5.8S
ITS 2
matK-3’trnK intron
psbA-trnH spacer
trnT-trnL spacer
Length
287-294 bp
164 bp
227-229 bp
774 bp
475-545 bp
1261-1500 bp
Mean GC content Pairwise divergence
52.9%
0 – 0.095
54.3%
0
57.1%
0 – 0.092
33.3%
0 – 0.022
20.9%
0 – 0.089
21.1%
0 – 0.047
ten indels from this region were re-coded as binary characters. The psbA-trnH intergenic
spacer varied from 475 to 545 bp in length within Tarasa. This spacer had the lowest
mean GC content (20.9%), but the greatest pairwise divergence values (0-8.9%) among
the three chloroplast regions sequenced. Variation within the psbA-trnH spacer consisted
of nucleotide substitutions, indels, and a 30 bp inversion which was excluded from the
phylogenetic analyses (see below). Three insertion-deletion events were re-coded as
binary characters.
The aligned data matrix of the three chloroplast regions contained 3331 base
pairs. The majority of the sequence variation among taxa was in the form of nucleotide
substitutions, although a total of 13 (3 in psbA-trnH and 10 in trnT-trnL) insertiondeletion (indels) events were coded as binary characters. A 30 bp perfect (no internal
nucleotide substitutions) inversion in the psbA-trnH spacer was omitted from the
analyses as it was found to occur homoplastically (as determined by mapping of the
inversion onto the phylogeny). No significant biases in base composition were detected
in the chloroplast data set (Table 3.5). The calculated g1 value for 10,000 randomly
generated trees using the chloroplast data matrix was –1.0705 [critical g1 values for 25
53
Table 3.5
Test for base composition bias of analyzed sequences (for entire data set
including outgroups). Values given are mean base frequency, degrees of
freedom (df), and P value (a value greater than 0.05 indicates that there is
not a significant difference among the taxa in the data set).
Region
ITS
matK-3’trnK intron
psbA-trnH spacer
trnT-trnL spacer
Table 3.6
A
0.17504
0.32057
0.36859
0.41458
C
0.29006
0.13570
0.07962
0.09909
G
0.27989
0.19728
0.12893
0.11440
T
0.25501
0.34644
0.42286
0.37193
df
153
126
126
126
P
1.00
1.00
1.00
0.12
Results from the Independent Length Difference (ILD) Test (Farris 1994)
for the molecular regions sequenced (ITS, matK-3’trnK intron, psbA-trnH
spacer, and trnT-trnL spacer). Data partitions were defined in PAUP* and
tested for homogeneity. The null hypothesis (that the data partitions are
homogenous) was rejected at the 0.05 level.
Comparison
matK-psbA-trnT
matK-psbA
psbA-trnT
matK-trnT
ITS-all chloroplast regions
P
0.02
0.20
0.27
0.03
0.01
Reject the null?
Yes
No
No
Yes
Yes
taxa and 250 variable characters are -0.09 (p=0.01), -0.08 (p=0.05)] (Hillis and
Huelsenbeck 1992).
The results of the ILD test for both the nuclear and chloroplast regions are
compiled in Table 3.6. The null hypothesis (that the data partitions were homogenous)
could not be rejected only for the matK-psbA (p=0.20) and psbA-trnT comparison
(p=0.27). The remaining analyses detected significant heterogeneity between the data
54
partitions (matK-psbA-trnT, p=0.02; matK-trnT, p=0.03; ITS-all chloroplast, p=0.01).
Although significant heterogeneity was detected in the three chloroplast regions, when
analyzed separately each region did not produce radically conflicting phylogenies.
Therefore, the three regions were combined for phylogenetic analyses. The ITS and
chloroplast data sets were analyzed separately.
The results for the three topological incongruence tests (Compare-2, Templeton
test, and Kishino-Hasegawa test) are compiled in Table 3.8. The null hypothesis for the
Compare-2 test (that the difference in tree lengths is what would be expected from
random data) could not be rejected (p=0.18). The Templeton and Kishino-Hasegawa
tests rejected the null hypothesis (that the data fit either tree equally well) of the ITS data
fitting as well as the chloroplast data onto the chloroplast phylogeny, p<0.0001 for both
tests. However, the null could not be rejected for both tests when the chloroplast data
was constrained to fit the ITS phylogeny (p=0.8236 and p=0.7946, respectively).
Chloroplast phylogeny reconstruction
Under parsimony, 238 characters in the combined chloroplast matrix were
informative (gaps coded as binary characters), 2775 characters were constant and 287
were variable but not parsimony-informative. The matK-trnK intron contributed 31
parsimony informative characters, the psbA-trnH spacer contained 64, and the trnT-trnL
intergenic region had 157 parsimony informative characters. An heuristic search as
previously described resulted in 15 most parsimonious trees of 619 steps.
The
consistency index (CI) excluding uninformative characters was 0.713, the retention index
(RI) was 0.873, and the rescaled consistency index (RC) was 0.734. Figure 3.2 is one of
the 15 most parsimonious trees (with the best likelihood score) and Figure 3.3 is the strict
consensus of these 15 trees.
55
For the chloroplast data set, constraining all traditionally circumscribed Tarasa
species to monophyly resulted in a single most parsimonious (MP) tree of length 675
(Table 3.7). Excluding Tarasa mandonii, T. machupicchensis, and T. spiciformis from
this constraint yielded 18 MP trees of length 639. Finally, constraining the tetraploid
species of Tarasa to form a monophyletic clade resulted in 2 MP trees of length 648. The
log likelihood scores for the best unconstrained tree was -8221.76602; the scores for the
three constraints were -8477.16295, -8321.25021, and -8368.71974, respectively. Each
chloroplast-based constraint tree was rejected as statistically different from the empirical
tree (p<0.001).
56
Figure 3.2
One of 15 most parsimonious trees based on chloroplast sequence data
(length=619, CI=0.713, RI=0.873, RC=0.735). Branch lengths are given
above the branches. Dashed branches collapse in the strict consensus.
Diploid (2n=10) perennial species of Tarasa are shown in blue, diploid
annual species are shown in red, and tetraploid annuals are shown in green.
The 2n=12 group is shown in orange. An asterisk following a taxon name
indicates inferred ploidy level (see page 50 for details). Letters following
taxon names indicate a separate population sampled.
57
Figure 3.3
Strict consensus of 15 most parsimonious trees based on chloroplast
sequence data. Bootstrap support (above 50) for 100 replicates is shown
below the branches. Diploid (2n=10) perennial species of Tarasa are shown
in blue, diploid annual species are shown in red, and tetraploid annuals are
shown in green. The 2n=12 group is shown in orange. An asterisk
following a taxon name indicates inferred ploidy level (see page 50 for
details). Letters following taxon names indicate a separate population
sampled.
58
Table 3.7
Results from constraint analyses using parametric bootstrapping on the ITS and chloroplast data sets. The first
constraint forced all traditionally circumscribed Tarasa to monophyly; the second constraint was as the first but
excluded the n=6 group of Tarasa machupicchensis, T. mandonii, and T. spiciformis; the third constraint forced
monophyly of all tetraploids (chromosome number known and inferred based on morphology and geography –
see text for details). The empirical tree length differences (=constrained-unconstrained) were compared to a
distribution of tree length differences generated from 100 simulated data sets. The null hypothesis (that the
constraint tree is the true tree) was rejected when the observed tree length difference was greater than 95% of the
simulated tree length differences. *For the chloroplast unconstrained tree, gaps were coded as missing.
Dataset
Description
Chloroplast Tree length
Number MP trees
Reject the null?
Likelihood score of
best tree
ITS
Tree length
Number MP trees
Reject the null?
Likelihood score of
best tree
No constraints
All Tarasa
619*
2
675
1
Yes (p<0.001)
-8477.16295
Tarasa (except
All tetraploids
n=6 group)
(known and suspected)
639
648
18
2
Yes (p<0.001)
Yes (p<0.001)
-8321.25021
-8368.71974
374
10
Yes (p<0.001)
-3231.85298
362
82
Yes (p<0.001)
-3178.10681
-8221.76602
348
7
-3148.67849
59
366
26
Yes (p<0.001)
-3241.67284
Table 3.8
Results of three incongruence tests as conducted in PAUP*. All tests
compared the strict consensus of the most parsimonious ITS trees and the
strict consensus of the most parsimonious chloroplast trees. The null
hypothesis that the data fit either tree equally well was rejected at the 0.05
level.
Test
Compare-2 test
Templeton test
Data set
Chloroplast
ITS
Chloroplast
Kishino-Hasegawa ITS
Chloroplast
Constraint
P
ITS strict consensus tree
0.13
Chloroplast strict consensus tree <0.0001
ITS strict consensus tree
0.8236
Chloroplast strict consensus tree <0.0001
ITS strict consensus tree
0.7946
ITS sequence characteristics
ITS sequences (ITS1, 5.8S, and ITS2) were obtained for the previously mentioned
taxa (Appendix C). Amplification of the ITS region yielded a single product in each case
as revealed by gel electrophoresis. No intraspecific ITS variation was evident for the
following taxa: Tarasa albertii, T. capitata, T. humilis, T. operculata, T. tenella, and T.
thyrsoidea.
The three populations of Tarasa antofagastana surveyed for this study
possessed differing ITS sequences and, therefore, all were retained for the phylogenetic
analyses. Variation within the ITS region consisted of nucleotide substitutions and small
indels (1-4 bp in length). Sequence statistics for species of Tarasa are compiled in Table
3.4. Within Tarasa, ITS1 sequence length varied from 286-294 base pairs and ITS2
from 227-229 bp. The 5.8S was invariable in length within Tarasa and the outgroups at
164 bp. Mean GC content in ITS1, 5.8S, and ITS2 were 52.9%, 54.3%, and 57.1%,
respectively. Pairwise divergence values between species of Tarasa were 0-9.5% for
ITS1, 0% for 5.8S, and 0-9.2% for ITS2. The data set (including outgroups) was not
60
found to contain significantly biased base composition among the taxa (p=1.00) (Table
3.5). The calculated g1 value for 10,000 randomly generated trees from ITS data matrix
was –0.508 [critical g1 value for 25 taxa and 100 variable characters = -0.12 (p=0.01), 0.10 (p=0.05)] (Hillis and Huelsenbeck 1992).
Seventeen taxa of Tarasa contained polymorphic sites (nucleotide positions in
which 2 or more peaks were apparent in the chromatogram) in the directly sequenced ITS
products, while twelve Tarasa taxa did not contain any polymorphisms in the ITS region.
Polymorphic sites were present in diploid annuals and perennials as well as many
tetraploid annual species. Conspecific individuals typically had the same number of
polymorphic sites, although they varied as to position in the alignment (e.g., as in Tarasa
operculata and Tarasa thyrsoidea). The three South American species of Sphaeralcea
included in this study all contained polymorphisms in the ITS, while the two North
American species had homogenous repeats.
Two of the three Nototriche species
sequenced contained polymorphisms in this region.
After analyzing the cloned ITS sequences for changes in conserved regions,
several sequences were discarded.
The observed changes included nucleotide
substitutions or single base-pair deletions in the angiosperm-conserved regions of ITS1
and ITS2, and the 5.8S (Liu and Schardl 1994, Hershkovitz and Zimmer 1996, Buckler
and Holtsford 1996, Mayol and Rosselló 2001). Nucleotide substitutions were usually
from G→A or C→T. In some cases, cloned sequences contained nucleotide substitutions
that were not apparent in the original, directly sequenced products. These sequences
were also removed before phylogenetic analyses were conducted. Typically, sequences
with deviations in one conserved region also had excess changes in the other regions.
After initial phylogenetic analyses were conducted, if clones from the same individual
clustered together in the ITS phylogeny, then only a single clone was retained for the
61
remaining analyses (e.g., T. marinii, T. meyeri, T. rhombifolia, T. tenella, T. tenuis, T.
urbaniana).
Therefore, in most cases, from the 5-10 clones sequenced for each
individual only two were considered functional copies (although three sequences were
retained for Tarasa geranioides).
No recombination events were apparent (i.e., no significant global fragments were
detected) among the ITS sequences as determined by GeneConv (Sawyer 1999).
ITS phylogeny reconstruction
The aligned length of the ITS region was 703 characters: 516 characters were
constant, 59 were parsimony uninformative, and 128 were parsimony informative. The
ITS 1 region was the most variable with 75 parsimony informative characters, while ITS
2 contained 51 and the 5.8S had only 2.
Parsimony searches as described above
recovered 7 most parsimonious (MP) trees of 348 steps, with a consistency index
(excluding uninformative characters) of 0.608, a retention index of 0.836, and a rescaled
consistency index of 0.568. Figure 3.4 is one of these MP trees (with the best likelihood
score) and Figure 3.5 is the strict consensus.
Table 3.7 compares the results from the unconstrained and constrained
phylogenetic analyses. Constraining all traditionally circumscribed Tarasa to monophyly
resulted in 10 most parsimonious (MP) trees of length 374. Excluding Tarasa mandonii,
T. machupicchensis, and T. spiciformis from this constraint yielded 82 MP trees of 362
steps. Finally, constraining the known and suspected tetraploid species of Tarasa to a
monophyletic clade resulted in 26 MP trees of length 366. The log likelihood score for
the best unconstrained tree was -3148.67849, while the scores for the three constraints
were -3231.85298, -3178.10681, and -3241.67284, respectively. For all three tests, the
null hypothesis (that the constraint tree was the true tree) was rejected (p<0.001).
62
Figure 3.4
One of 7 most parsimonious trees based on ITS sequence data (length=348,
CI=0.6084, RI=0.8360, RC=0.5677). Branch lengths are shown above the
branches. Dashed branches collapse in the strict consensus. Diploid
(2n=10) perennial species of Tarasa are shown in blue, diploid annual
species are shown in red, and tetraploid annuals are shown in green. The
2n=12 group is shown in orange. An asterisk following a taxon name
indicates inferred ploidy level (see page 50 for details). Letters following
taxon names indicate a separate population sampled; a number following the
name indicates clone number.
63
Figure 3.5
Strict consensus of 7 most parsimonious trees based on ITS sequence data.
Bootstrap support (above 50) for 100 replicates is shown below the
branches. Diploid (2n=10) perennial species of Tarasa are shown in blue,
diploid annual species are shown in red, and tetraploid annuals are shown in
green. The 2n=12 group is shown in orange. An asterisk following a taxon
name indicates inferred ploidy level (see page 50 for details). Letters
following taxon names indicate a separate population sampled; a number
following the name indicates clone number.
64
DISCUSSION
Taxon sampling
Regrettably, each of the three species of Tarasa lacking from the phylogenetic
study are known only from their type specimens and were not found during the two
collecting trips made to South America. The type localities of Tarasa reichei and Tarasa
umbellata in southern Chile were discovered to be converted to pine and eucalyptus
plantations (pers. obs.). The type of Tarasa martiniana, the third species not sampled,
was collected in northern Chile in mid-March 1968. During the ultimate collecting trip to
South America, we collected in that region in early February 2000. As the summer rains
were delayed that year and the species is an annual, it is likely that we were merely too
early to collect it.
Urocarpidium albiflorum is not a Tarasa
Both the ITS and chloroplast phylogenies support the placement of Urocarpidium
albiflorum with species of Fuertesimalva and not with Tarasa, as proposed by Fryxell
(1996). The presence of a prominent apical awn on the mericarps of U. albiflorum and
species of Tarasa is a convergent morphological character.
Tarasa species and
Urocarpidium albiflorum/Fuertesimalva species are markedly different in other
morphological characteristics, most notably mericarp pubescence (stellate pubescent in
the former and glabrous in the latter) and calyx and leaf pubescence (stellate stipitate in
Tarasa and hirsute in U. albiflorum/Fuertesimalva species).
It is uncertain if
Urocarpidium merits recognition as a monotypic genus sister to Fuertesimalva. Further
phylogenetic investigation of the Fuertesimalva species is warranted.
65
Chloroplast phylogeny
As previously discussed in Chapter 2, Tarasa was found not to be monophyletic
in the ITS phylogeny of the tribe Malveae, a finding which is corroborated by the
chloroplast-based phylogeny (Figures 3.2 and 3.3). The n=6 group composed of Tarasa
machupicchensis, T. mandonii, T. spiciformis, and Malacothamnus chilensis is placed
basal to the clade containing the other species of Tarasa, as well as Nototriche and
Sphaeralcea. As the n=6 group possesses both a distinct chromosome number and
overall morphology, a new genus will be created to accommodate these species (Tate in
prep).
Even with the removal of the n=6 group, Tarasa remains a non-monophyletic
assemblage. Interestingly, in the chloroplast phylogeny (Figures 3.2 and 3.3), several
Argentinian species of Tarasa cluster with the South American species of Sphaeralcea.
Two of these Tarasa species, T. albertii and T. humilis, most closely resemble
Sphaeralcea in terms of morphology and geographic distribution. In South America,
Sphaeralcea is restricted to Chile and Argentina at elevations up to 3000 m. Although
not currently sympatric (both Tarasa species are found at higher elevations in the Andes
than Sphaeralcea species), these two species occupy the southern-most range of the
genus (Figure 3.1). The other taxa belonging to this “southern” clade in the chloroplast
phylogeny are Tarasa trisecta (a diploid annual) from northwestern Argentina and
Tarasa tenuis (a tetraploid annual) from southern Peru. These two species are sister taxa
in both the ITS and chloroplast phylogeny, which may account for the anomalous
placement of a Peruvian taxon in an otherwise Argentinian clade. In any case, it appears
that these lineages have experienced gene flow at some time in Argentina through a
maternal parent.
66
The monophyly of Tarasa is further violated by the placement of Nototriche in
the chloroplast phylogeny. In the strict consensus (Figure 3.3), Nototriche forms a wellsupported clade as part of a larger clade containing Tarasa rhombifolia (perennial from
Cuzco, Peru), T. marinii (annual from Cuzco, Peru), T. congestiflora (annual from
Arequipa, Peru), and T. pediculata (annual from northern Chile). Because primarily
Peruvian taxa appear to be most closely related to Nototriche (in both the nuclear and
chloroplast trees), Peru could have been an important center of gene flow and/or
speciation among these taxa. The three perennial species of Nototriche included were
from varying geographic regions (Argentina, Bolivia, and Peru).
Unfortunately, no
annual species of Nototriche were available for this study; these may prove essential for
further unraveling the relationship between these two high elevation genera. The species
of Tarasa that share with Nototriche the unusual morphological feature of fused stipules,
petiole, and peduncle are T. marinii, T. nototrichoides, T. odonellii, and T. urbaniana. As
these four species do not form a monophyletic clade, this character appears to have arisen
multiple times.
One of the most surprising results from phylogeny reconstruction was the nonmonophyly of the tetraploid species. This outcome was unexpected, particularly because
of their unusual and shared morphological features of white or lilac flowers, petals 1-3
(rarely 6-7) mm in length, fewer than 20 anthers, and highly lobed leaves.
The
phylogeny suggests that the tetraploids have been formed multiple times with recurrent
evolution toward a similar morphology. The results of the parametric bootstrapping
(constraint #3) in particular lend support to this conclusion. The chloroplast sequence
data have been somewhat informative as to the potential maternal parent (or lineage) of
the Tarasa tetraploids (Table 3.9). For example, the tetraploid Tarasa antofagastana was
placed near to Tarasa meyeri (diploid annual) and Tarasa latearistata (suspected diploid
67
annual) in the phylogeny.
The sequences for these species are strikingly similar,
especially when one considers that almost 3 kb of the chloroplast was sequenced. This
result suggests that Tarasa antofagastana is a recent polyploid. Surprisingly, the three
populations of Tarasa antofagastana sampled did not cluster together in the chloroplast
phylogeny. The closest population geographically (T. antofagastanaB from Tucuman,
Argentina) to the diploid annuals was the most closely related to it and, in fact, that
population was identical to Tarasa meyeri for the three chloroplast regions sequenced.
The other two populations (Mexico and Jujuy, Argentina) sampled have apparently
accumulated only 1 or 2 more nucleotide substitutions, respectively.
Based on the
chloroplast phylogeny results, Tarasa trisecta is a potential parent of Tarasa tenuis, as is
Tarasa cardenasii a potential parent of T. odonellii.
The pattern of diploid parent-
tetraploid derivative is not as clear for the other Tarasa tetraploid species, however. If
we assume that the most closely related diploid species in the chloroplast phylogeny is
the maternal parent for the tetraploid of interest (Brochmann et al. 1996, Ge et al. 1999,
Popp and Oxelman 2001), then the same diploid taxon would be implicated for the origin
of different tetraploids (Table 3.9).
An allopolyploid origin for the tetraploids is
strengthened if the geographic distribution of the diploids is also considered (Figure 3.1).
Table 3.10 indicates the geographic areas where the diploids (perennials and annuals) are
sympatric (based on mapping of herbarium specimens). The data indicate that in several
localities (particularly in southern Bolivia and adjacent Argentina) different diploids are
sympatric, which may have provided the opportunity for gene exchange. Unfortunately,
however, crossing data is not currently available for these species.
68
Table 3.9
Hypothesized relationships for tetraploid species of Tarasa (chromosomally known and inferred) based on
nuclear (paternal parent) and chloroplast (maternal) phylogeny reconstructions. An asterisk indicates a tetraploid
for which the chromosome number was inferred. Hypotheses are based on the most closely related diploid
species to the tetraploid in each phylogeny.
Tetraploid species
T. antofagastana
T. cerrateae
T. congestiflora*
T. corrugata*
T. geranioides
T. marinii*
T. nototrichoides *
T. odonellii
T. pediculata*
T. tarapacana
T. tenella
T. tenuis
T. urbaniana
Chloroplast phylogeny
T. meyeri (diploid annual)
T. capitata (diploid perennial)
T. rhombifolia (diploid? perennial)
T. cardenasii (diploid annual)
T. cardenasii (diploid annual)
T. rhombifolia (diploid? perennial)
T. meyeri (diploid annual)
T. cardenasii (diploid annual)
T. rhombifolia (diploid? perennial)
T. capitata (diploid perennial)
T. meyeri (diploid annual)
T. trisecta (diploid annual)
T. capitata (diploid perennial)
69
ITS phylogeny
T. heterophylla (diploid annual)
T. cardenasii (diploid annual)
T. rhombifolia (diploid? perennial)
T. cardenasii (diploid annual)
T. latearistata (diploid? annual)
T. rhombifolia (diploid? perennial)
?
?
T. cardenasii (diploid annual)
T. cardenasii (diploid annual)
?
T. trisecta (diploid annual)
T. cardenasii (diploid annual)
The placement of the diploid perennial Tarasa capitata within a clade of annual
diploids and tetraploids was surprising since the majority of the diploid perennial species
occupy more basal positions in the Tarasa clade. This species is widespread along the
eastern Cordillera of the Andes from Peru to Argentina and may be a taxon from which
the diploid annuals were ultimately derived. An evolutionary trend from perennial to
annual habit is common in many groups (Grant 1981), although reversals to a perennial
habit have been documented (e.g., Barrett et al. 1996). Given the relatively large number
of autapomorphies on the Tarasa capitata branch in the chloroplast phylogeny (Figure
3.2), this taxon is likely much older than the diploid or tetraploid annuals.
ITS phylogeny
In the ITS phylogeny (Figures 3.4 and 3.5), the n=6 group forms a well-supported
clade basal to Nototriche, Sphaeralcea, and the remaining species of Tarasa.
Sphaeralcea forms a monophyletic clade separate from the remaining species of Tarasa.
However, the next branch in the ITS tree contains Tarasa albertii and T. humilis. On the
basis of morphology, these two species are clearly sister taxa and are distinct from other
species of Tarasa as well as Sphaeralcea.
Inspection of the seven most parsimonious ITS phylogenies reveals a single
nucleotide substitution which places various species of Tarasa sister to a strongly
supported Nototriche clade. These species include Tarasa tenuis and Tarasa trisecta
(sister taxa as previously mentioned), Tarasa marinii (annual) and Tarasa rhombifolia
(perennial) both from eastern Peru (Cuzco), and Tarasa congestiflora (annual) from
southern Peru (Arequipa). In the strict consensus of the ITS trees (Figure 3.5), the
species of Nototriche are a monophyletic group in an otherwise unresolved clade
70
Table 3.10 Areas of sympatry between diploid species of Tarasa that are potential tetraploid parents (see Table 3.9).
Perennial species are indicated by an asterisk. n/a indicates no apparent areas of overlap in geographic
distribution based on mapping of 1400 herbarium specimen localities.
Tarasa capitata*
Tarasa cardenasii
Tarasa heterophylla
Tarasa latearistata
Tarasa
capitata*
--
Tarasa
cardenasii
N. Bolivia
Tarasa
heterophylla
S. Bolivia
NW Argentina
Tarasa
latearistata
S. Bolivia
NW Argentina
--
S. Bolivia
--
S. Bolivia
S. Bolivia
--
Tarasa meyeri
Tarasa trisecta
Tarasa rhombifolia*
71
Tarasa
meyeri
S. Bolivia
NW Argentina
C Argentina
(Cordoba)
S. Bolivia
S. Bolivia
S. Bolivia
NW Argentina
--
Tarasa
trisecta
NW
Argentina
Tarasa
rhombifolia*
Cuzco, Peru
n/a
n/a
n/a
n/a
n/a
n/a
NW
Argentina
--
n/a
n/a
--
containing most species of Tarasa. Again, additional sampling from Nototriche would
likely aid in determining the origins of this unusual genus.
One of the most notable features of the ITS phylogeny is the fact that ITS clones
from the same individual do not cluster together. For example, the two diploid perennials
Tarasa operculata and Tarasa thyrsoidea have ITS copies which are more closely related
to each other (paralogs) than to ITS copies from the same species (orthologs). Tarasa
operculata is found along the coast from central Peru to northern Chile at elevations
between 1000-3000 m, while Tarasa thyrsoidea is endemic to a few coastal lomas in
Peru (elevation <500 m). The two species are often mistaken for one another, but differ
in several morphological characters. The former has conspicuous stipules and bracteoles
several mm in length and white flowers with purple nectar guides densely arranged in
axillary cymes, while the latter has vestigial stipules and reduced “papery” bracteoles and
lavender flowers ‘spread out’ along the axis of the cyme. The pattern recovered in the
ITS phylogeny for these two species is a classic example of gene divergence predating
speciation (Li 1997). As such, one of the pairs of sequences represents a paralogous copy
of the ITS. Further molecular analysis of the nuclear ribosomal repeats would be needed
to determine which is the dominant copy.
A more complicated scenario exists for the tetraploid species, however. The
presence of multiple ITS types (and polymorphisms in the directly sequenced products)
indicates that hybridization and/or allopolyploidization events have occurred. Given the
low sequence diversity among the species (0-5.6%) and the relatively young geological
area that the species inhabit (the high Andes), these events have most likely been recent.
Concerted evolution is expected to homogenize repeats belonging to gene families
(Zimmer et al. 1980).
However, this process may fail to homogenize the nuclear
ribosomal repeats when 1) hybridization (or allopolyploidization) is recent, 2) when the
72
parents of the hybrid have repeats located at different loci and interlocus concerted
evolution fails, or 3) when a hybrid is asexual (Baldwin et al. 1995, Sang et al. 1995,
O’Kane, Jr. et al. 1996, Fuertes Aguilar et al. 1999). In the case of Tarasa, not all
tetraploids possess multiple ITS copies (e.g., Tarasa antofagastana A, T. congestiflora,
T. corrugata, T. nototrichoides, T. odonellii, T. pediculata). Presumably, these taxa
either have ITS repeats which are already homogenized or they are autotetraploids.
Given the contrasting species relationships which are recovered in the nuclear and
chloroplast phylogenies, the former seems a more plausible explanation. For the taxa
which still possess multiple, seemingly functional, ITS types (e.g., Tarasa antofagastana
B and C, T. cerrateae, T. geranioides, T. latearistata, T. tarapacana), concerted
evolution has failed or has not had sufficient time to homogenize the repeats. The
placement of the Tarasa antofagastana B and C population clones appears logical as they
cluster with conspecific clones (albeit from separate populations) or with geographically
proximal taxa (T. antofagastanaC2 from Mexico with T. geranioides1 also from
Mexico). The two cloned sequences from Tarasa cerrateae are members of the same
clade, which might indicate that concerted evolution is currently working to homogenize
the copies. Tarasa geranioides is the only species for which three presumably functional
ITS clones were recovered.
Each of these three sequences maintain the conserved
regions in ITS1 and ITS2, as well as the 5.8S. One copy (T. geranioides1) clusters with
Tarasa antofagastanaC2 with which it is geographically close.
Another copy (T.
geranioides2) clusters with Tarasa odonellii, a closely related taxon in the chloroplast
phylogeny, and the third copy is sister to Tarasa latearistata1 (a suspected diploid). The
latter taxon is the only diploid (chromosome number inferred based on morphology) with
multiple ITS types that did not cluster together in the phylogeny. The second copy (T.
latearistata2) is sister to Tarasa meyeri, to which it is closely related in the chloroplast
73
phylogeny. Although morphology would indicate that this species is diploid, it could
very well be a hybrid or an allopolyploid. Tarasa tarapacana, a known tetraploid,
similarly did not have clones that clustered together. One copy (T. tarapacana1) is
placed in a clade with several other tetraploids and the diploid Tarasa meyeri, while the
other copy (T. tarapacana2) is placed in a clade consisting only of tetraploids.
As a biparentally inherited marker, one would expect that recent hybrids or
allopolyploids would possess two ITS types and that their phylogenetic placement would
indicate parental origins (i.e., one copy would cluster with the maternal parent and the
other copy with the paternal parent).
Likewise, when nuclear and chloroplast
phylogenies are incongruent, hybrid or polyploid origins may be inferred from the
biparental and maternal trees, respectively (Kim and Jansen 1994, Wendel and Doyle
1998). In this group of species, however, the data seem to indicate that each diploid
species (particularly the annuals) may have been repeatedly involved in the formation of
a different tetraploid. For example, Tarasa meyeri is a potential maternal parent of the
tetraploid Tarasa antofagastana (Table 3.9) based on the chloroplast sequence data. In
the nuclear ITS phylogeny, the most closely related diploid is Tarasa heterophylla, which
fruit morphology would also support as a parent. In that same ITS clade, however, are
other tetraploids (e.g., T. cerrateae, T. geranioides) which may also have been derived
from T. heterophylla (or T. latearistata). Further support to this hypothesis is given by
the current geographic distribution of the diploid species.
In multiple locations,
particularly in northwestern Argentina and southern Bolivia, different diploid annuals are
sympatric.
Since the tetraploids occupy a much wider geographic range than the
diploids, the molecular data seem to be more informative as to parental origins than is the
current geographic distribution. Complicating matters further is the observation that the
Tarasa tetraploids are morphologically more similar to each other than they are to the
74
diploid species. Consequently, they do not display the classic pattern of morphological
intermediacy between parental species.
Again, although the origins for all of the
tetraploid species are still not definitively known, the molecular data has revealed the
extensive reticulate evolution that has occurred within this group.
Hypothesis testing and incongruent phylogenies
Because unexpected phylogenetic results arose from the chloroplast and ITS data
sets, statistical tests to assess the reliability of the hypotheses were conducted.
Parametric bootstrapping was performed to test alternative a priori hypotheses of
monophyly for Tarasa as currently circumscribed, Tarasa without the n=6 group, and the
tetraploids forming a monophyletic clade. For both the chloroplast and ITS data sets, and
for each constraint, the results were highly significant (p<0.001) that the recovered trees
consisting of the non-monophyletic groups are reliable (Table 3.7), thus strengthening the
conclusions drawn from these phylogenetic estimates.
Additional tests to evaluate the seemingly incongruent chloroplast and ITS trees
were also conducted (Table 3.8). The compare-2 results indicate that the difference in the
chloroplast and ITS topologies is no greater than expected by chance. The results of the
Templeton and Kishino-Hasegawa tests indicate that the chloroplast data fit onto the ITS
phylogeny as well as the ITS data, but that the reverse case is not true (the ITS data do
not fit onto the chloroplast tree). This asymmetry indicates that some evolutionary
process has produced these topologies (Doyle 1992, Wendel and Doyle 1998). Given the
accumulated geographical, morphological, and cytological information, reticulation
seems to have been a major factor in shaping the evolution of these species.
75
Recovering the organismal phylogeny: Gene trees versus species trees
When using molecular sequence data to reconstruct phylogenies, it is important to
remember that the resulting hypothesis of relationships reflects the history of the DNA
molecule (or more precisely, the particular region of the molecule utilized) and not
necessarily the history of the organisms under study (Doyle 1992). Certain evolutionary
processes, such as lineage sorting, hybridization/introgression, and gene duplication may
obscure true species relationships (Doyle 1992, Rieseberg and Wendel 1993, Wendel and
Doyle 1998). Current methods of phylogenetic reconstruction enforce bifurcating trees
and, as a result, reticulate events may not be apparent without supportive evidence.
Concerted evolution may also distort species relationships, as was found in Gossypium
(Wendel et al. 1995). The Gossypium allotetraploids have ITS sequences which have
been homogenized toward one parental copy or the other. As a result, the ITS phylogeny
was not concordant with known species relationships based on other data (Wendel et al.
1995). Thus, caution must be exercised when interpreting molecular phylogenies. In
particular, when seeking explanations for incongruent trees (from multiple data sources)
or unexpected phylogenetic results, an approach which considers geography,
morphology, and cytology is necessary.
In the case of Tarasa, the molecular phylogenies interpreted along with
morphological and geographical data indicate a complex evolutionary history which has
involved repeated allopolyploidization. The seemingly recent evolution of these species
may have been associated with the final uplift of the Andean Cordillera during the
Pleistocene. As evidence, the most basal lineages of the genus are occupied by lower
elevation species and the most derived positions are occupied by the highest elevation
species (including Nototriche which is only found above 4000 m).
The current
geographic distribution of the diploid annual species indicates that northwestern
76
Argentina and Bolivia have been areas of sympatry where allopolyploidization events
may have occurred. The phylogenetic, geographic, and morphological data point to
recurrent encounters among these species which have resulted in these unusual polyploid
species.
77
Chapter 4: Multiple origins of polyploidy and breeding system
evolution in Tarasa (Malvaceae)
ABSTRACT
A previous molecular phylogenetic investigation of the genus Tarasa Philippi
(Malvaceae) revealed multiple origins of polyploidy and morphological convergence of
the tetraploid species. Breeding systems for diploid and tetraploid species were inferred
using pollen/ovule (p/o) ratios and/or measured directly in greenhouse grown plants. The
diploid perennial species exhibited the highest p/o ratios (xenogamous), followed by the
diploid annuals (xenogamous to facultatively xenogamous), and the tetraploid annuals
with the lowest p/o ratios (autogamous or facultatively xenogamous). The tetraploid
annuals had a significantly lower p/o ratio than the diploid annuals and perennials when
all taxa were considered. None of the diploid perennial species grown in the greenhouse
were self-compatible, whereas all of the tetraploid annuals were autogamous. Only one
diploid annual was available for study in the greenhouse, and it produced self-seed
although the resulting progeny were weak (likely the result of inbreeding depression).
Not only do the tetraploid species have fewer anthers per flower, but they also produce
fewer pollen grains per anther as compared to both the diploid annuals and perennials.
Pollen diameters (as measured under light microscopy) of the tetraploids were also
compared to the diploid species. Contrary to a central tenet of polyploidy, the tetraploid
species of Tarasa have significantly smaller pollen grains than both the diploid annuals
and perennials.
This size difference also contradicts Cruden’s (1977) finding of a
negative correlation between pollen/ovule ratio and pollen size. When all species of
Tarasa are considered, the overall correlation between these two variables is also
78
positive. The tetraploid species of Tarasa occupy the highest elevation habitats in the
Andes. Their unusual and shared morphology was likely influenced by adaptations to the
harsh high altitude habitats, a reduction in pollinators at such altitudes, and adaptations
that accompany an autogamous breeding system.
INTRODUCTION
Following polyploidization, many plant taxa undergo alterations in morphological
and reproductive features. Likewise polyploidy is often correlated with habit and habitat.
Table 4.1 lists the characters most often associated with polyploidy and compares them to
the tetraploid species of the genus Tarasa (Malvaceae). For example, polyploids often
occupy a wider geographic range than their diploid relatives and are frequently found in
disturbed or marginal habitats (Ehrendorfer 1980).
Polyploidy is common at high
latitudes and altitudes (Löve and Löve 1949, Diers 1961, Löve and Löve 1974, Stebbins
1984) and in previously glaciated areas, particularly those affected by the advance and
retreat cycles during the Pleistocene (Ehrendorfer 1980). Many polyploids are perennial
in habit, which may confer the ability to delay reproductive efforts in the absence of a
suitable mate (Stebbins 1940). Similarly, a breakdown of self-incompatibility
Table 4.1
Typical attributes of polyploid taxa as compared to Tarasa polyploids
POLYPLOID FEATURES
Occupy marginal or disturbed habitats over
a greater area
Perennial habit
Self-compatible → Autogamous
Larger cell size compared to diploids
(pollen and guard cells)
TARASA TETRAPLOIDS
Occupy highest elevations in the Andes in
disturbed habitats
Annual habit
Autogamous
Smaller cell size compared to diploids
(pollen, fide Tressens 1970)
79
mechanisms often accompanies polyploidization (Stebbins 1950, 1957, Levin 1983,
Richards 1997, Soltis and Soltis 2000, Rosquist 2001, but see Cook and Soltis 1999).
Because chromosome doubling is expected to increase cell size, polyploids should have
larger cells (as exemplified by pollen grains and guard cells) than their diploid relatives
(Stebbins 1940, 1950). As Table 4.1 outlines, the tetraploid species of Tarasa contradict
two of the main dogmas associated with polyploids: they are annuals and several species
possess smaller pollen grains than the diploids (Tressens 1970). Polyploids often also
display a myriad of other phenotypic and physiological differences relative to their
progenitors including changes in enzyme phenotype, hormone levels, secondary
chemistry, and meiotic/mitotic rates (Stebbins 1950, Tal 1980, Levin 1983, Ramsey and
Schemske 1998, Otto and Whitton 2000).
A molecular phylogenetic analysis of Tarasa (Malvaceae) and related genera
revealed that the tetraploid species have been formed multiple times (Chapter 3). The
polyphyly of the tetraploids was particularly unexpected because of their strikingly
similar and unusual morphologies. Typically, Tarasa tetraploids are sprawling to erect
annuals (up to 0.5 m in height) with white or pale lavender colored flowers, petals 1-3
mm in length, 5-20 anthers, and highly lobed leaves. Conversely, the diploid species are
easily distinguished from one another. They are annuals (up to 1 m) or perennials (up to
1.5 m) typically with lavender or magenta colored flowers, petals 5-10 mm in length,
anthers numbering 30-100, and shallowly divided leaves. Molecular and geographic data
indicate that the polyploids are most likely the result of hybridization (followed by
polyploidization) between various sympatric diploid annual species. Although previous
phylogenetic investigations have demonstrated that multiple origins are common for a
particular polyploid species (Doyle et al. 1990, Brochmann et al. 1992, Wallace and
Jansen 1995, Cook et al. 1998, Doyle et al. 1999, Segraves et al. 1999, Soltis and Soltis
80
1999, Sharbel and Mitchell-Olds 2001), multiple occurrences in Tarasa are particularly
interesting due to the recurring evolution of several morphological features.
The unusual and shared morphology of the Tarasa tetraploids is therefore
apparently the result of convergent evolution and not a reflection of common ancestry.
One explanation for the recurrent morphological similarity of the tetraploids could be a
switch from outcrossing to autogamy in the high elevation habitats that the tetraploids
occupy. Pollen/ovule (p/o) ratios have traditionally been used as an indicator of the
relative degree of outcrossing versus selfing in plants (Cruden 1977). In many unrelated
plant taxa, pollen/ovule ratios have been shown to be negatively correlated with selffertilization, pollen size, and the ratio of the stigmatic area to the pollen-bearing area on
the pollinator (Cruden 1977, Cruden 2000, Cruden and Miller-Ward 1981). The two
main objectives for this study were to 1) infer breeding system indirectly from
pollen/ovule ratios and directly from greenhouse studies and 2) examine pollen size and
morphology for the diploid and tetraploid species to determine if pollen size relationships
hold across all polyploids and if a correlation exists with breeding system.
MATERIALS AND METHODS
Pollen/Ovule ratios
To determine pollen grains per flower, the most mature, unopened bud on an
inflorescence was removed from a selected herbarium specimen (Table 4.2 lists the
vouchers). When possible, two or more individuals from separate populations were
sampled to account for geographic variability. For each individual, the numbers of
anthers and styles in a single flower were recorded. The total number of pollen grains in
an anther was directly counted for three anthers per flower. Because the time to anther
maturity varies along the staminal column, one anther was selected from each of the top,
81
middle, and bottom of the column. Each anther was macerated in 10 µL of lactophenolaniline blue (Kearns and Inouye 1993) in a 0.5 µL “PCR” tube and then vortexed for 30
seconds. The pollen/lactophenol mixture was placed on a microscope slide and all grains
in that anther were counted at 200x magnification on a Leitz microscope. In the case that
the anthers had dehisced before the bud opened, the occurrence was noted and a younger
bud was selected from the same specimen.
To calculate P/O ratios, the number of grains per anther was averaged for the
three anthers. The mean number of grains per anther was then multiplied by the total
number of anthers in that flower. The total number of grains was then divided by the
number of styles because in Tarasa the carpels are uniovulate and equal to the number of
styles.
Pollen/ovule ratios were also log transformed in order to compare them to
Cruden’s (1977) extensive breeding system data set.
Chromosome number was inferred for species of Tarasa for which direct counts
were not available. All perennial species were considered to be diploid (2n=10), except
Tarasa spiciformis which is considered 2n=12 on the basis of similar morphology to
Tarasa machupicchensis and T. mandonii (both 2n=12). Annuals with magenta or purple
petals longer than 3 mm, anthers numbering more than 30, and a restricted geographic
distribution were considered diploid (2n=10). Annuals with white or pale lavender
flowers, petal length less than 3 mm, anthers fewer than 30, and a wide geographic
distribution were assumed to be tetraploid (2n=20).
For statistical analyses, species of Tarasa were grouped according to ploidy level
or habit and ploidy level. Single-factor ANOVA was conducted to test for significance
of mean anther number, mean number of grains per anther, and mean pollen/ovule ratio.
For each category, two groups were evaluated: only species with known chromosome
82
Table 4.2
Vouchers used in pollen/ovule ratio analysis
Species
Tarasa albertii
Tarasa albertii
Tarasa antofagastana
Tarasa antofagastana
Tarasa antofagastana
Tarasa antofagastana
Tarasa antofagastana
Tarasa capitata
Tarasa capitata
Tarasa capitata
Tarasa cardenasii
Tarasa cardenasii
Tarasa cerrateae
Tarasa cerrateae
Tarasa congestiflora
Tarasa congestiflora
Tarasa corrugata
Tarasa corrugata
Tarasa geranioides
Tarasa heterophylla
Tarasa heterophylla
Tarasa humilis
Tarasa humilis
Tarasa latearistata
Tarasa machupicchensis
Tarasa machupicchensis
Tarasa mandonii
Tarasa mandonii
Tarasa mandonii
Tarasa marinii
Tarasa meyeri
Tarasa meyeri
Tarasa nototrichoides
Voucher and location
Bartlett 19452 (US)
Tate et al. 74 (TEX)
Tate et al. 66 (TEX)
Pisano & Venturelli 1945 (SGO)
Krapovickas & Shinini 30755 (F)
Semper 433 (NY)
Tate et al. 92 (TEX)
Tate et al. 63 (TEX)
Lewis 35295 (MO)
Cabrera 22629 (CTES)
Ehrich 462 (US)
Nee 44773 (NY)
Chanco 40 (TEX)
Chanco 29 (TEX)
Pennell 13168 (NY)
Arroyo 84-578A (CONC)
Nunez 7485 (MO)
Tate et al. 110 (TEX)
Hinton et al. 8326 (LL)
Bastion 863 (US)
Kiesling 3731 (MO)
Ruiz Leal 24662 (CTES)
Tate et al. 69 (TEX)
Fiebrig 3096 (US)
Timana & Tate 3771 (TEX)
Chavez Alfaro 3453 (MO)
Fortunato et al. 1893 (F)
Luteyn & Dorr 13790 (TEX)
Cardenas 3112 (US)
Vargas 5067 (CAS)
Krapovickas et al. 22021 (GH)
Krapovickas & Seijo 47841 (TEX)
Feuerer 10570 (CTES)
83
Table 4.2
continued
Species
Tarasa nototrichoides
Tarasa odonellii
Tarasa odonellii
Tarasa operculata
Tarasa operculata
Tarasa pediculata
Tarasa pediculata
Tarasa rhombifolia
Tarasa spiciformis
Tarasa tarapacana
Tarasa tarapacana
Tarasa tarapacana
Tarasa tenella
Tarasa tenella
Tarasa tenuis
Tarasa tenuis
Tarasa thyrsoidea
Tarasa thyrsoidea
Tarasa trisecta
Tarasa trisecta
Tarasa urbaniana
Tarasa urbaniana
Voucher and location
Franken 338 (LPB)
Krapovickas 22029 (CTES)
Pierotti 1038 (NY)
Tate & Timana 62 (TEX)
Tate et al. 95 (TEX)
Teillier 4103 (SGO)
Belmonte 97052 (CONC)
Tate et al. 109 (TEX)
Stock & Horton s.n. (F)
Matthei & Rodriguez 258 (CONC)
Venturi 8733 (US)
Tate et al. 80 (TEX)
Krapovickas & Seijo 47838 (TEX)
Matthei & Rodriguez 219 (CONC)
Lopez 5546 (CTES)
Vargas 9263 (MO)
Ferreyra 5553 (TEX)
Tate et al. 107 (TEX)
Tate et al. 91 (TEX)
Venturi 4302 (US)
Leiva & Leiva 488 (TEX)
Krach 8410 (NY)
number and all species with chromosome number known and inferred. Two post-hoc
ANOVA tests were also performed:
Fisher’s PLSD (a less stringent test) and
Bonferroni/Dunn (a more conservative test).
Self-compatibility in greenhouse grown plants
Plants were grown in the greenhouse at The University of Texas at Austin from
seed collected in the field or sampled from herbarium specimens. Among the species
studied were five diploid perennials (Tarasa albertii, T. capitata, T. humilis, T.
operculata, and T. thyrsoidea), one diploid annual (Tarasa trisecta), and six tetraploid
84
annuals (Tarasa antofagastana, T. geranioides, T. odonellii, T. tarapacana, T. tenella, T.
urbaniana). Plants were allowed to self pollinate without any mechanical manipulation.
If the plants did not produce selfed seeds, the flowers were manually pollinated with self
and outcross pollen (in this case from plants from the same parent). Diploid flowers were
emasculated before the bud opened, but attempts to emasculate buds of some tetraploids
proved unsuccessful as the minute flowers were extremely sensitive to disturbance (the
flowers did not mature/open after handling). The number of fully mature seeds per
schizocarp was counted for fifty fruits on a single plant. Mean seed set was calculated as
the proportion of mature seeds produced relative to the total possible.
Pollen characteristics
The same individuals used for pollen/ovule ratios were examined for several
pollen morphological characteristics (Table 4.2).
The traditional acetolysis method
(Erdtman 1960) for preparing pollen proved too harsh for Tarasa grains, as was
bleaching in a weak (10%) solution for a short period of time. Instead, the grains were
washed for 15 minutes in 800 µL acetone to remove any residual pollenkitt. After the
grains settled to the bottom of the tube, the acetone was decanted and the grains allowed
to air-dry overnight. Pollen grains were stored in 80% ethanol until studied.
A drop of pollen/ethanol mixture was placed on a slide and the ethanol allowed to
evaporate. The grains were mounted in glycerin jelly with acid fuschin stain (Kearns and
Inouye 1993). Slides were rendered permanent by sealing the edge of the cover slip with
clear fingernail polish.
Using light microscopy, twenty-five grains per individual were evaluated for
shape, size, aperture number, and other gross morphological features. The measurements
of the grains excluded the spines and were recorded for both polar and equatorial
85
diameters. Pollen diameter measurements (polar axis) were used to calculate surface area
and these data were plotted against log pollen/ovule ratio (Cruden 1977, Cruden and
Miller-Ward 1981). A subset of taxa was selected for a scanning electron microscopy
(SEM) study to represent the morphological diversity among the species.
SEM
preparation consisted of placing a droplet of pollen/ethanol suspension on a conductive
carbon tab (EM Sciences) mounted on an SEM stub and allowing the ethanol to
evaporate (at 60º C) . The samples were sputter coated on a Ladd Benchtop Sputter
Coater with 60/40 Ag/Pd target at 2.5 kV (20mA) for 75 seconds (coating thickness of 15
nm). Grains were examined on a Philips 515 scanning electron microscope at 13 kV,
bias set to 2, spot size of 40 nm, and a working distance of 30 mm (tilt approximately
15º). Digital images were captured with a SEMICAPS 2000 system and exported as tif
files.
RESULTS
Pollen/ovule ratios
A total of 80 individuals representing 27 of the 30 species of Tarasa were
evaluated for pollen/ovule ratios. The three species not sampled are only known from the
type specimens and no other specimens were available for study. Table 4.3 summarizes
the mean number of anthers, mean number of pollen grains per anther, mean number of
ovules, and mean pollen/ovule ratio for each species studied. Graphical representations
of each of these factors are shown in Figures 4.1-4.3. Table 4.4 compiles the mean and
standard deviations for anther number, number of grains per anther, and P/O ratio for the
ploidy level and habit categories. The results of the ANOVA are also given in Table 4.4
for these categories. ANOVA detected significant differences for mean anther number,
mean number of grains per anther, and mean pollen/ovule ratio for the category of ploidy
86
level as well as when ploidy level and habit were considered (P<0.0001 in each case).
Table 4.5 gives the results from the post-hoc ANOVA Fisher’s PLSD and
Bonferroni/Dunn tests.
Mean anther number per flower varied greatly between the 2n=20 tetraploid (520) and the 2n=10 diploid (24-102) species (Table 4.3). For chromosome number both
known and inferred, the difference in anther number was significant under both Fisher’s
PLSD and Bonferroni/Dunn between diploid (2n=10) annuals and perennials (p=0.0032,
and p=0.0022, chromosome number known and inferred, respectively), diploid annuals
and tetraploids (p=0.0039 and p=0.0004, respectively), and diploid perennials and
tetraploids (p<0.0001 for both categories).
Mean number of grains per anther was not significantly different between diploid
annuals and diploid perennials. When the chromosomally known diploid annuals and
tetraploid annuals were compared, the differences were significant under Fisher’s PLSD
only (p=0.0117). When all taxa were included, the mean number of grains per anther was
significantly different under both tests (p=0.0005). The diploid perennials and tetraploid
annuals significantly differed in number of grains per anther in both categories for both
tests (p=0.0002 chromosomally known, p<0.0001 all taxa).
For mean pollen/ovule ratio, a significant difference was detected between the
diploid annuals and perennials only under Fisher’s PLSD test: p=0.0349 and p=0.0434
for chromosome number known and inferred, respectively.
When p/o ratios were
compared between the diploid annuals and the tetraploids, a significant difference was
found only when all taxa were considered and only under Fisher’s PLSD (p=0.0123).
When only the chromosomally known taxa were tested, the difference was not significant
under either test (p=0.0511). However, a significant difference was detected between the
87
Table 4.3.
Results of pollen/ovule ratio analysis for species of Tarasa. An asterisk indicates inferred chromosome number
on the basis of morphology and geographic distribution (see text for explanation). Means and standard
deviations (in parentheses) are given for number of grains per anther, number of anthers per flower, and number
of ovules per flower. Breeding system was determined by comparing the empirical log p/o ratios to values
reported by Cruden (1977) (X = xenogamy, FX = facultative xenogamy, FA = facultative autogamy, and OA =
obligate autogamy) – see text for details.
Species
T. cardenasii
T. heterophylla
T. latearistata
T. meyeri
T. trisecta
T. albertii
T. capitata
T. humilis
T. operculata
T. rhombifolia*
T. thyrsoidea
T. machupicchensis
T. mandonii
T. spiciformis*
T. antofagastana
Individuals 2n
sampled
2
2
1
2
2
2
3
2
2
1
2
2
3
1
5
10
10
10
10
10
10
10
10
10
10
10
12
12
12
20
Habit
Flower
color
Petal
Length
(mm)
Number of
grains/anther
Number of
anthers/flower
Annual
Annual
Annual
Annual
Annual
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Annual
Lilac
Lilac
Lilac
Lilac
Lilac
Magenta
Lilac
Magenta
White
Lilac
Lilac
Rose-pink
Rose-pink
Rose-pink
White
2-3
5-7
7-8
8-10
5-7
10-15
5-15
8-10
8-10
9-10
10
12-15
20-30
10-15
3-4
143.50 (8.25)
305.33 (60.81)
348.00
258.17 (5.42)
382.50 (20.03)
309.83 (47.85)
350.00 (111.66)
322.50 (18.15)
376.17 (156.27)
265.33
441.50 (20.98)
275.67 (19.80)
510.33 (62.49)
330.00
102.20 (43.29)
24 (2)
38 (2)
40
30 (0)
60 (0)
55 (4)
47 (13)
69 (2)
76 (8)
40
102 (9)
72 (5)
67 (7)
60
15 (4)
88
Number
Mean P/O
of
ratio
ovules/flower
10 (1)
9 (1)
10
12 (1)
9 (1)
10 (0)
13 (3)
12 (0)
11 (1)
10
13 (1)
9 (3)
8 (1)
10
10 (2)
337.23
1272.22
1392.00
673.48
2086.36
1704.08
1265.38
1840.94
2722.73
1061.33
3584.98
2190.02
4459.87
1980.00
155.43
Log P/O
ratio
Breeding
system
2.53
3.10
3.14
2.83
3.32
3.23
3.10
3.27
3.44
3.03
3.55
3.65
3.30
3.34
2.19
FX
X
X
FX
X
X
X
X
X
X
X
X
X
X
OA
Table 4.3
Species
T. cerrateae
T. congestiflora*
T. corrugata*
T. geranioides
T. marinii*
T. nototrichoides
T. odonellii
T. pediculata*
T. tarapacana
T. tenella
T. tenuis
T. urbaniana
continued
Individuals 2n
sampled
2
2
2
1
1
2
2
2
3
2
2
2
20
20
20
20
20
20
20
20
20
20
20
20
Habit
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Flower Petal
color Length
(mm)
White
White
Pink
White
White
White
White
White
White
White
Lilac
White
Number of
Number of
grains/anther anthers/flower
1.5-2 159.00 (37.24)
2.5-3 70.50 (14.38)
2.5-3 123.83 (69.53)
1.5-2
32.00
6-7
245.67
1-2 227.33 (51.38)
2-3
59.50 (9.19)
2-3
62.50 (6.84)
1.5-2 136.33 (37.55)
1.5-2 110.17 (47.38)
6-7 198.83 (100.17)
1.5-2 92.50 (86.03)
89
10 (0)
10 (0)
17 (5)
5
20
5 (0)
5 (0)
10 (1)
9 (1)
8 (4)
19 (5)
7 (3)
Number
Mean P/O
of
ratio
ovules/flower
10 (3)
8 (0)
7 (1)
9
8
9 (1)
10 (2)
9 (1)
8 (0)
10 (1)
8 (1)
8 (1)
159.00
88.13
291.89
17.78
614.17
126.30
31.32
69.85
159.06
86.97
490.46
86.33
Log P/O
ratio
Breeding
system
2.20
1.95
2.47
1.25
2.79
2.10
1.50
1.84
2.20
1.94
2.69
1.94
OA
OA
FA/FX
OA
FA/FX
OA
OA
OA
OA
OA
FA/FX
OA
diploid perennials and tetraploid annuals for both categories and under both post-hoc tests
(p=0.0002 for chromosomally known, p<0.0001 for all taxa).
According to Cruden (1977), a log transformed pollen/ovule score of 3.59-3.71
indicates xenogamy, 2.76-2.86 indicates facultative xenogamy, 2.09-2.21 indicates
facultative autogamy, and a score of 1.38-2.21 indicates obligate autogamy. The scores
for Tarasa suggest that the diploid annuals (log p/o 2.53-3.32) are facultatively
xenogamous to xenogamous, the diploid perennials (log p/o 3.03-3.55) are xenogamous,
and the tetraploid annuals (log p/o 1.25-2.79) are obligately autogamous, facultatively
autogamous, or facultatively xenogamous.
Mean number of anthers per flower
90
80
70
60
50
40
30
20
10
0
2n=20 Annuals
2n=10 Annuals
2n=10 Perennials
2n=12 Perennials
Chromosome number and habit
Figure 4.1
Histogram of mean number of anthers per flower for all species of Tarasa
analyzed.
90
500
Mean number of grains
per anther
400
300
200
100
0
2n=20 Annuals
2n=10 Annuals
2n=10 Perennials 2n=12 Perennials
Chromosome number and habit
Figure 4.2
Histogram depicting mean number of grains per anther for all species of
Tarasa analyzed.
4500
4000
Mean pollen/ovule ratio
3500
3000
2500
2000
1500
1000
500
0
2n=20
Annuals
2n=10
Annuals
2n=10
Perennials
2n=12
Perennials
Chromosome number and habit
Figure 4.3
Histogram of mean pollen/ovule ratio for all species of Tarasa analyzed.
91
Table 4.4
Means and standard deviations (in parentheses) of anther number per flower, number of grains per anther, and
pollen/ovule ratio for ploidy level and habit categories in Tarasa (see Table 4.3 for details). Significance levels
as detected by ANOVA are also given for these categories.
Category
Mean anther number Mean number of grains
per flower
per anther
Mean P/O ratio
Log
P/O ratio
3.06
3.31
3.46
2.26
ALL TAXA
2n=10 annuals
2n=10 perennials
2n=12 perennials
2n=20 annuals
ANOVA
Ploidy level
Ploidy level and habit
38 (14)
65 (23)
66 (6)
11 (5)
287.500 (93.005)
344.222 (60.656)
372.000 (122.839)
124.643 ( 66.695)
1152.258 (677.848)
2029.907 (955.277)
2876.630 (1375.141)
182.822 (179.837)
F=30.012 ,P<0.0001
F=32.957, P<0.0001
F=24.012, P<0.0001
F=16.819, P<0.0001
F=22.241, P<0.0001
F=18.557, P<0.0001
38 (16)
70 (21)
70 (4)
10 (5)
272.375 (100.040)
360.000 (52.266)
393.000 (165.930)
111.316 (53.416)
1092.323 (767.261)
2223.622 (926.958)
3324.945 (1605.026)
148.294 (149.129)
F=17.926, P<0.0001
F=24.490, P<0.0001
F=18.076, P<0.0001
F=14.441, P=0.0001
F= 15.071, P=0.0002
F=14.588, P=0.0001
KNOWN PLOIDY LEVEL ONLY
2n=10 annuals
2n=10 perennials
2n=12 perennials
2n=20 annuals
ANOVA
Ploidy level
Ploidy level and habit
92
3.04
3.35
3.52
2.17
Table 4.5
P-values for mean anther number per flower, number of grains per anther,
and P/O ratio as determined by Fisher’s PLSD and Bonferroni/Dunn post
hoc ANOVA for diploid (2n=10 only shown) and tetraploid (2n=20) species
of Tarasa. Calculated P values were the same for each test. Significant
differences are indicated as follows: * indicates a p-value significant under
Fisher’s PLSD at the 0.05 level, ** indicates a p-value significant under
Bonferroni/Dunn at the 0.05 level, *** indicates significant difference under
both tests (0.05 level).
Comparison
ALL TAXA
diploid annuals, diploid perennials
diploid annuals, tetraploid annuals
diploid perennials, tetraploid
annuals
KNOWN PLOIDY LEVEL ONLY
diploid annuals, diploid perennials
diploid annuals, tetraploid annuals
diploid perennials, tetraploid
annuals
Anther
number
per flower
Number of
grains
per anther
P/O ratio
P=0.0022***
P=0.0004***
P<0.0001***
P=0.2364
P=0.0005***
P<0.0001***
P=0.0434*
P=0.0123*
P<0.0001***
P=0.0032***
P=0.0039***
P<0.0001***
P=0.1271
P=0.0117*
P=0.0002***
P=0.0349*
P=0.0511
P=0.0002***
Self seed set
Table 4.6 summarizes the results from self seed set for species of Tarasa grown in
the greenhouse. None of the diploid perennial species (Tarasa albertii, T. capitata, T.
humilis, T. operculata, and T. thyrsoidea) were found to be self-compatible.
The
tetraploid annuals were all autogamous, but to varying degrees as represented by mean
seed set (0.53-0.87).
93
Table 4.6
Self seed set in greenhouse grown individuals of Tarasa. Plants were grown
in the greenhouse at The University of Texas at Austin. The number of fully
mature seeds per schizocarp was counted for fifty schizocarps on a single
plant. Autogamous seed set is the proportion of mature seeds produced to
the number of carpels present. Standard deviations are given in parentheses.
An asterisk indicates an inferred chromosome number on the basis of
morphology and geographic distribution (see text for details).
Species
Tarasa trisecta
Tarasa machupicchensis
Tarasa antofagastana
Tarasa geranioides
Tarasa nototrichoides*
Tarasa odonellii
Tarasa tarapacana
Tarasa tenella
Tarasa urbaniana
Species
Tarasa albertii
Tarasa capitata
Tarasa humilis
Tarasa operculata
Tarasa thyrsoidea
2n
Habit
10 Annual
12 Perennial
20 Annual
20 Annual
20 Annual
20 Annual
20 Annual
20 Annual
20 Annual
2n
Habit
10
10
10
10
10
Perennial
Perennial
Perennial
Perennial
Perennial
Autogamous seed set
0.42 (0.20)
0.71 (0.25)
0.70 (0.23)
0.75 (0.20)
0.53 (0.23)
0.66 (0.24)
0.87 (0.14)
0.85 (0.20)
0.63 (0.26)
Seed set from
manual self-pollinations
0
0
0
0
0
Pollen characteristics
Table 4.7 lists the results of pollen characterization utilizing light microscopy.
All grains within Tarasa were spherical to sub-spherical in shape with dimorphic spines.
The larger spines are positioned on basal cushions, both of which are variable in size
between the species (Figure 4.4). The small spines are scattered between the larger
94
Table 4.7
Results of pollen measurements utilizing light microscopy. Twenty-five
grains were measured per individual; when possible 2 or more individuals
were sampled to represent a species. An asterisk inidicates an inferred
chromosome number based on morphology and geographic distribution (see
text for details). Values given are the mean and standard deviation for the
polar and equatorial axis, and the approximate number of apertures.
Species
2n
Habit
Polar axis
(µm)
T. cardenasii
T. heterophylla
T. latearistata
T. meyeri
T. trisecta
T. albertii
T. capitata
T. humilis
T. operculata
T. rhombifolia*
T. thyrsoidea
T. machupicchensis
T. mandonii
T. spiciformis*
T. antofagastana
T. cerrateae
T. congestiflora*
T. corrugata*
T. geranioides
T. marinii*
T. nototrichoides
T. odonellii
T. pediculata*
T. tarapacana
T. tenella
T. tenuis
T. urbaniana
10
10
10
10
10
10
10
10
10
10
10
12
12
12
20
20
20
20
20
20
20
20
20
20
20
20
20
Annual
Annual
Annual
Annual
Annual
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Perennial
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
Annual
41.55 (0.01)
36.15 (0.64)
38.88 (2.01)
40.22 (1.21)
38.43 (1.03)
37.93 (0.24)
39.90 (1.61)
39.61 (0.97)
42.49 (4.52)
38.15 (2.21)
39.12 (6.44)
40.19 (3.10)
39.90 (1.67)
33.31 (2.11)
38.57 (1.20)
34.34 (1.67)
32.99 (1.07)
38.06 (0.43)
35.26 (1.58)
40.08 (1.32)
32.44 (2.88)
35.16 (2.10)
34.46 (5.32)
37.99 (0.50)
35.43 (0.04)
37.98 (2.80)
37.73 (3.01)
95
Equatorial
axis
(µm)
44.73 (1.51)
39.04 (0.27)
40.89 (1.76)
42.88 (1.01)
41.91 (2.70)
40.65 (1.27)
42.13 (2.18)
42.96 (1.00)
45.21 (4.21)
40.67 (2.00)
41.65 (7.52)
43.37 (3.51)
43.13 (2.85)
36.00
40.76 (1.78)
37.16 (2.67)
36.30 (0.83)
40.17 (1.37)
38.88 (2.06)
44.00 (2.83)
36.52 (1.95)
38.42 (2.49)
37.11 (5.11)
40.51 (0.06)
38.66 (0.09)
40.81 (3.27)
40.34 (3.60)
Number of
apertures
~6
~7
4
4
3-4
3-4
~5
3-4
3-4
~5
3-4
3-4
3-4
3-4
6-8
~6
4-5
~6
~8
~6
~6
~8
~6
~6
~8
~6
8
Figure 4.4
Scanning electron micrographs of pollen grains for 6 species of Tarasa
(Malvaceae) demonstrating variation in grain size and spine size and
morphology. Magnification and scale bars are given in the lower right
corner of each panel. Panels A-C: Tetraploid annual species, panels D-E:
Diploid perennials, panel F: Diploid annual.
96
Table 4.8
Means and standard deviations for pollen grain size (polar and equatorial
diameters) for species of Tarasa studied. P values from ANOVA are given
for the categories of ploidy level and ploidy level and habit for all taxa
(chromosome number inferred) or chromosomally known taxa only.
Mean polar
diameter (µm)
Mean equatorial
diameter (µm)
39.046 (2.027)
39.533 (1.645)
36.192 (2.351)
41.890 (2.131)
42.212 (1.714)
39.203 (2.182)
ALL TAXA
Diploid annual
Diploid perennial
Tetraploid annual
ANOVA
Ploidy level
F= 5.479, P=0.0110 F= 4.699, P=0.0190
Ploidy level and habit F= 3.558, P=0.0300 F= 3.026, P=0.0501
KNOWN ONLY
Diploid annual
Diploid perennial
Tetraploid annual
39.087 (2.339)
39.810 (1.676)
36.557 (1.662)
42.140 (2.375)
42.520 (1.720)
39.442 (1.349)
ANOVA
Ploidy level
F= 7.157, P=0.0060 F=8.611 , P=0.0029
Ploidy level and habit F= 4.707, P=0.0165 F= 5.462, P=0.0097
spines and are barely visible with the light microscope. Figure 4.4 illustrates the gross
morphological differences among some of the species.
Differences in aperture (all
colporate) number were also apparent (Table 4.7), although the precise number was at
times difficult to assess since the apertures also seem to vary in size. When the number
of apertures was 3-4, the colpi were equatorially distributed on the grain. Pollen with 5
or more apertures present had the colpi spirally arranged around the grain.
Pollen sizes were variable both within and between species of Tarasa (Table 4.7).
Within a species, the pollen grain size typically varied only a few micrometers, with a
few exceptions (e.g., Tarasa thyrsoidea and T. pediculata). The mean polar diameter
97
ranged in the diploid (2n=10) annual taxa from 36.15-41.55 µm, the diploid perennials
from 37.93-42.49 µm, and the tetraploid annual species from 32.44-40.08 µm. Figure 4.5
is a histogram of the mean pollen diameter (polar axis) for these three categories.
Significant differences were detected by ANOVA for mean pollen grain size (both polar
and equatorial diameters) for the ploidy level and ploidy level and habit categories (Table
4.8). These values were significant when all taxa were considered (e.g., ploidy level:
polar diameter P=0.0110, equatorial diameter P=0.0190), as well as when only the
chromosomally known taxa were included (e.g., ploidy level: polar diameter P=0.0060;
equatorial diameter P=0.0029).
Post-hoc ANOVA tests revealed significant differences between some of the
categories (Table 4.9).
When chromosomally known and all taxa were evaluated,
Fisher’s PLSD detected significant difference between diploid annuals and tetraploid
annuals for polar diameter (p=0.0339 known, p=0.0301 all taxa) and equatorial diameter
(p=0.0183, known and p=0.0390 all).
A significant difference was detected under
Fisher’s PLSD between the diploid perennials and the tetraploids for polar diameter
(p=0.0057 known, p=0.0083 all taxa) and equatorial diameter (p=0.0054 known,
p=0.0155 all). Bonferroni/Dunn returned a significant difference only for the difference
between diploid perennials and the tetraploids for the chromosomally known only
category (p=0.0057 polar diameter, p=0.0054 equatorial diameter).
Figure 4.6 depicts the relationship between log surface area (pollen size) and log
pollen/ovule ratio for the species of Tarasa studied. Correlation coefficients for the
tetraploids, diploid annuals, and diploid perennials were 0.6224, -0.6031, and 0.2454,
respectively.
98
Table 4.9
Significance levels for mean pollen diameter (polar axis) as determined by
Fisher’s PLSD and Bonferroni/Dunn post hoc ANOVA for diploid (2n=10
only shown) and tetraploid (2n=20) species of Tarasa. Calculated P values
were the same for each test. Significant differences are indicated as follows:
* indicates a p-value significant under Fisher’s PLSD at the 0.05 level, **
indicates a p-value significant under Bonferroni/Dunn at the 0.05 level, ***
indicates significant difference under both tests (0.05 level).
Comparison
Mean polar
diameter
Mean equatorial
diameter
ALL TAXA
diploid annuals, diploid perennials
P=0.7347
diploid annuals, tetraploid annuals
P=0.0301*
diploid perennials, tetraploid annuals P=0.0083*
P=0.8218
P=0.0390*
P=0.0155*
KNOWN PLOIDY LEVEL ONLY
diploid annuals, diploid perennials
P=0.5521
P=0.7382
diploid annuals, tetraploid annuals
P=0.0339*
P=0.0183*
diploid perennials, tetraploid annuals P=0.0057*** P=0.0054***
Mean pollen (polar)
diameter (µm)
45
40
35
30
2n=10 annuals
2n=10 perennials
2n=20 annuals
Ploidy level and habit
Figure 4.5
Histogram of mean pollen diameter (polar axis) in micrometers for the
categories of diploid annual, diploid perennial, and tetraploid annual.
99
Figure 4.6
Relationship between surface area of pollen grain (polar axis) and
pollen/ovule ratio for species of Tarasa studied (see Table 4.3). Tetraploid
annual species are shown in red, diploid annuals in light blue, and diploid
perennials in dark blue. Pearson correlation coefficients (r) for the
tetraploids, diploid annuals, and diploid perennials are 0.519, -0.698, and
0.296, respectively.
DISCUSSION
A shift in breeding system follows polyploidization in Tarasa
Following polyploidization, a shift from an outcrossing to a selfing breeding
system has been empirically shown in many taxa (Stebbins 1950, Grant 1981, Levin
1983, Richards 1997, Ramsey and Schemske 1998, but see Cook and Soltis 1999). The
tetraploid species of Tarasa follow this expectation and have each independently evolved
a morphology typical of autogamous plants yet atypical for polyploids. This shift to
100
autogamy in Tarasa has repeatedly involved an overall reduction in floral morphology
that includes smaller petals, fewer anthers per flower, and fewer grains produced by each
anther (Tables 4.4 and 4.5). Consequently, the difference in pollen/ovule ratios between
the diploids and tetraploids is striking (Figure 4.3). The best phylogenetic estimate for
the species indicates that the tetraploids were formed multiple times (Chapter 3) and were
likely derived from hybridization events between different pairs of diploid annuals. The
diploid annuals possess floral morphologies that appear to promote outcrossing (Table
4.3), including relatively large lavender petals (> 5 mm) with nectar guides, many
anthers, and the stigmas exserted several millimeters beyond the anther mass. The
pollen/ovule ratios indicate that the diploid annuals may be facultatively xenogamous
(Table 4.3 and 4.4).
They do appear to possess “leaky” self-incompatibility (only
represented by one species in the greenhouse), although apparently with the consequence
of inbreeding depression. Polyploidy is expected to buffer the effects of deleterious
alleles arising from inbreeding (Stebbins 1957, Charlesworth and Charlesworth 1987,
1995).
Not surprisingly, the tetraploids do not appear as susceptible to inbreeding
depression as the diploid annuals, since vigorous individuals were grown from
autogamous seed (data not shown).
Considered together, the phylogenetic and
reproductive data indicate that the “selfing” tetraploid morphology has recurrently
evolved from predominantly outcrossing diploids. In the evolutionary history of the
Tarasa species, a shift from a perennial to an annual habit likely occurred first and was
followed by increased self-compatibility in the diploid annuals. After the formation of
the tetraploid species, autogamy then became the predominate breeding system and those
morphological features typical of autogamous taxa (inconspicuous flowers, few anthers
and few grains produced per anther) evolved. Other phylogenetically based studies have
documented the rather labile nature of plant breeding systems (reviewed in Weller and
101
Sakai 1999). For example, a few other studies have detected multiple origins of selfing
within a genus, particularly in Eichhornia (Barrett 1995) and Gilia (Barrett et al. 1996).
However, the tetraploid species of Tarasa represent a particularly interesting case due to
the extensive nature of their convergent morphology and because they do not fit the
archetypal polyploid pattern.
Plants found at high elevations are typically smaller in stature and floral features
than plants growing at lower elevation (Clausen et al. 1940). The reduction in floral
features in Tarasa tetraploids is not surprising in this regard but the size difference
between diploids and tetraploids is maintained in the greenhouse. Many factors have
likely contributed to the evolution of this unusual and recurring floral form exhibited by
the tetraploid species of Tarasa [inconspicuous white or lavender flowers, petals less than
3 mm (but 6-7 mm in 2 species) in length, and anthers numbering 5-20]. The novel
genetic combinations (resulting from allopolyploidization) appear to have allowed the
tetraploids to exploit higher elevation habitats in the high Andes that were not previously
occupied by the diploid species. Consequently, morphological adaptations to the high
altitude habitats would have had a selective advantage.
In particular, because the
tetraploids are annuals, a breeding system/morphology which offers reproductive
assurance in an environment where pollinators are scarce and the growing season is short
would be essential.
Polyploidy is particularly common among taxa found at high
latitudes and high altitudes (Löve and Löve 1949, Diers 1961, Löve and Löve 1974,
Stebbins 1984). Arroyo et al. (1981, 1982, 1985) and Arroyo and Squeo (1990) in a
series of community studies of the high Chilean Andes demonstrated a relationship
between breeding system and pollinator abundance (frequent autogamy at high elevations
where pollinators are scarce). Abiotic factors in particular were shown to influence the
latter (Arroyo et al. 1981 1982, 1983). Low pollinator abundance may have been a pre102
existing element in shaping the tetraploid morphology subsequent to invasion of the high
elevation habitats. However, a phylogenetic component may have also contributed to the
plasticity of the floral characters observed in the tetraploids (conferred by the diploid
annuals).
Tarasa tetraploid pollen size contradicts breeding system and ploidy level
expectations
The data presented here for pollen size in Tarasa contradict previous findings for
both a negative correlation between pollen/ovule ratios and pollen size (Cruden and
Miller-Ward 1981, Mione and Anderson 1992, Cruden 2000) and a positive correlation
between ploidy level and pollen size (Stebbins 1940, 1950). In an autogamous breeding
system, fewer pollen grains are produced and these are typically larger than in
outcrossing relatives (Cruden and Miller-Ward 1981, Cruden 2000). This relationship
has been attributed to a trade-off in sexual allocation between size and number (Charnov
1982, Queller 1984) and/or to floral morphological characters, in particular, style length
and stigmatic depth (Cruden and Lyon 1985, Cruden 2000 and references therein). In
Tarasa, the tetraploids possess an autogamous breeding system where fewer pollen grains
are produced per anther, but the pollen grains are significantly smaller than the diploids
where xenogamy is favored (p=0.003 under Bonferroni/Dunn) (Table 4.9 and Figure 4.6).
When habit and ploidy level are considered among the species, the tetraploids are not
significantly different in pollen size from the diploid annuals under the more conservative
post-hoc ANOVA (Bonferroni/Dunn p= 0.0399 chromosomally known only, p=0.0301
all taxa), but are significantly smaller than the diploid perennials (p=0.0057 known,
p=0.0083 all taxa). Interestingly, regression analysis indicates a positive relationship
between pollen size (as surface area) and pollen/ovule ratio for the tetraploids (r=0.519)
103
and diploid perennials (r=0.296), but the diploid annuals exhibit the expected negative
correlation between the two variables (r=-0.698). Since the tetraploids have smaller
flowers (thus shorter styles) than the diploid annuals and perennials, a structural
component also likely contributes to the smaller pollen found in the tetraploids.
Chromosome drawings by Krapovickas (1960) indicate that the tetraploid chromosomes
are not smaller in size than the diploids.
Attributing pollen size to a single factor does not seem appropriate for most plant
species, especially in the case of Tarasa. Interrelated environmental and genetic factors
have likely contributed to the unexpected patterns observed here. Among the most
important considerations are 1) the tetraploids are primarily autogamous and are derived
from primarily xenogamous diploid annuals, and 2) the former have undergone a
morphological reduction in all floral characters probably as a reponse to a combination of
a shift in breeding system and to the harsh environment of the high Andes.
104
Appendix A. Malveae ITS alignment
ITS1 (1-355), ITS2 (356-623)
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
10
GTCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
??????????
GTCGAAACCT
GTCGAAAGCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCAAAACCT
-TCGAAACCT
GTCGAAACCT
-TCGAAACCT
GTCGAAAGCT
GTCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
GTCGAAAGCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCGAAATCC
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
-TCGAAACCT
20
30
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG C-ACATGACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AT--GACC
?????AGCAG A-AT--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAA ATAC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCC-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCC-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AY--GACC
GCCT-AGCAG A-AT--GACC
GCCT-AGCAG A-AT--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AC--AACC
GCCT-AGCAG A-AC--GACC
GCCT-AGCAG A-AT--GACC
GCCT-AGCAG A-AT--GACC
GCCT-AGCAG A-AT--GACC
GCCT-AGCAG A-AT--GACC
105
40
CGT-GAATGT
CGT-GAATGT
CGT-GAATGC
CGT-GAACGT
CGT-GAACGT
CGT-GAATGT
CGT-GAATGT
CGT-GAACAA
CCG-GAACAA
CGT-GAACGT
CGC-GAACGT
CGT-GAATGT
CGT-GAACGT
CGC-GAATGT
CGC-GAATGT
CGT-GAACGT
CGT-GAATGT
CGT-GAATGT
TGT-GAACGT
TGT-GAACGT
CGT-GAACGT
CGT-GAACAT
CGA-GAACGT
CGT-GAATGC
CGT-GAATGC
CGT-GAATGT
CGT-GAAYGT
CGT-GAATGT
CGT-GAACGT
CGT-GAACGT
CGT-GAACGT
TGT-GAATGC
CGT-GAATGC
CGC-GAACGC
CGT-GAATGT
CGC-GAACGT
CGT-GAATGT
CGT-GAACGC
CGT-GAACGT
AGT-GAACGT
CGT-GAACGT
CGT-GAACGT
CGT-GAACAT
CGT-GAACAT
50
60
GTTATCATAC AA--AACACC
GTTATCATAC AA--AACACC
GTTATCATAC AA--AACACC
GATATAAAAC AA--CTCAAC
TATATAAAAC AA--CTCAAC
GATATCAAAC AA--TCGAAT
GATATCAAAC AA--TCGAAT
GTTATCATAC AA--AACAGC
GTTATCAAAC AA--AACAAC
GATATCAAAC AA--CTCAAC
GTTATCATAC AA--AACAGC
GTTATCATAC AA--AACATC
GTTATTATAC AA--ATCACC
GTTATCATAC AA--AACACC
GTTATCATAC AA--AACACC
GTTATCAAAC AA--AACAAC
GTTACCATAC AA--AACAAC
GTTACCAAAC AA--AACAAC
GTTATCAAAC AA--CTAAAC
GTTATCAAAC AA--CTAAAC
GATATCAAAC AA--CTAAAC
GTTAATAAAC AA--AACAAC
GTTATAAAAC AA--CTCCTC
GTTATCAAAC AA--AACAGC
GTTATCAAAC AA--AACAGC
GTTACCAAAC AA--AACAAC
GATATCAAAC AA--CWAAAC
GATATCAAAC AA--CAAAAC
GTTATCGTAC AA--AACAAC
GATATCAAAC AA--CTCAAC
GATATCAAAC AA--CTCAAC
GTTATCATAC AA--AACAAC
GTTATCATAC AA--AACAAC
GTTG-CAAAC AA--CACC-G
GTTACCAAAC AA--AACAAC
GTTATCAAAC AA--CTCCAC
GTTATCATAC AA--AACATC
GTTATCAAAC AA---TAA-GATATCAAAC AA--CAAAAC
GATATCAAAC AA--CAAAAC
GATATTGAAC AA--CTCAAC
GATATTGAAC AA--CTCAAC
GATATCAAAC AA--CTCAAC
GATATCAAAC AA--CTCAAC
70
--GAGAGAG--GAGAGGG--GAGAGGG--GAGGGGG--GAGGGGG--GAGGGGGG
--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGAGTG--GGGAGGG--GAGGGGG--GATAGGG--GAGAGAG--GAGGGGG--GAGAGGG--GAGTGGG--GAGGAGG--GAGGAGG--GAGGGGG--GAGGGGG--GAGGAGGGAGAGAGGG--GAGAGGG--GAGTGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGAGGG--GAGAGGG--GAGGTGG--GAGTGGG--GAGGGG-A
--GAGAGGA--GAGGGC---GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG-
75
TGCGG
TGCGG
TGCGG
TGCGG
TGCGG
TGAGG
TGAGG
CGCGG
AGCAG
CGCGG
TGAGG
TGCGG
TGCGG
TGAGG
TGCGG
CGAGG
CGCGG
CGCGG
TGTGG
TGTGG
CGCGG
CGCGG
TGCGG
TGCGG
TGCGG
CGCGG
CGCGG
CGCGG
CGCGG
CGTAG
CGTAG
CGTTG
CGTTG
TGCGG
CGCGG
GGCGG
TGCGG
CGCGG
CGCGG
CGCGG
TGCGG
TGCGG
CGCGG
CGCGG
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
GTCGAAACCT
GTCGAAACCT
??????????
-------CCT
---------GTCGAAACCT
GTCAAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
GTCGAAACCT
------------------GTCGAAACCT
---------GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
GTCAAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
-TCGAAACCT
GTCGAAAC-T
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAACCCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
???GAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
-TCAAAACCT
-TCAAAACCT
-TCAAAACCT
-TCGAAACCT
GTCGAAACCT
GTCGAAACCG
GCCT-AGCAG
GCCT-AGCAG
??????????
GC-A-AGCAG
---------GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCTAAGCAG
GCCT-AGCAG
GCCA-AGCAG
----------CCT-AGCAG
GCCT-AGCAG
---------GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAA
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GC?T-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCTT-AGCAG
GCAT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCAT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
TCCT-AGCAG
A-AT--GACC
A-AC--GACC
??????????
A-AC--GACC
---C--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AT--GACC
A-AT--GACC
A-AC--GACC
---------A-AC--GACC
A-AC--GACC
---------A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--AACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AY--G-CC
A-AC--GACC
AAAC--GACC
A-AC--GACC
A-AC---ACC
A-AC--GACC
A-AC--GACC
A-AC--AACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
ATAC--GCCG
A-AC--GACC
ATAC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AT--GACC
ATAC--GACC
A-AT--GACC
A-AT--GACC
A-AT--GACC
A-GA--GACC
ATAC--GACC
---C--GACC
106
CGT-GAACGT
CGC-GAACGT
???-?AACGT
CGC-GAACGT
CGC-GAACGT
CGC-GAACGT
CGT-GAACGT
CGT-GAACGT
CGT-GAACGT
CGC-GAACGT
CGC-GAACGT
-----AACGT
CGC-GAACGT
CGC-GAACGT
-----AACGT
CGT-GAACGT
CGC-GAACGT
CGT-GAACGT
CGT-GAACGT
TGT-GAATGT
CGT-GAACGT
CGT-GAACGT
TGT-GAACAT
CGT-GAACGT
CGT-GAACGT
CGC-GAACGT
TGT-GAACGT
TGT-GAACGT
TGT-GAACGT
CGT-GAACGT
CGT-GAACGT
CGT-GAATGT
CGT-GAACGT
CGT--AATGT
CGT-GAACGT
AGC-GAATGT
CGT-GAATGC
CGT-GAATGT
TGT--AATGT
CGC-CAACGT
CGC-GAACGT
CGT-?AATGT
TGT-GAATGT
CGT-GCATGT
TGT-GAATGT
CGC-GAACGT
TGT-?AATGT
CGT-GAATGT
CGT-GAACAT
CGT-GAACGT
CGT-GAACAT
CGT-GAACAT
CGC-GAACGT
TGT-GAATGT
GATATCAAAC
GTTATCATAC
GATGTCAAAC
GTTATCGAAC
GATATCGAAC
GTTATCAAAC
GTTATCAAAC
GATATCATAC
GATATCAAAC
GATATCAAAC
GTTATCGAAC
GTTATCGACC
GTTATCGAAC
GTTATCGAAC
GATTTCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GTTATCAAAC
GTTATCATAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GTTATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATAAAAC
GATATCAAAC
GTTATCATAC
GTTATCAAAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCAACC
GTTATCAAAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
GTTATCAAAC
GTTATCATAC
GTTATCATAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GTTATCAAAC
GTTATCATAC
AA--TTGAAC
AA--AACAAC
ACAACCAGTC
AA--CCGATC
ACAAACAATC
AA--CTCCGC
AA--CTTTGC
AA--CTCCAC
AA--CAAAAC
TA----GA-C
AA--CCGATC
AA--CCGATC
AA--CCGATC
AA--CCGATC
AA--CCGA?C
AA--CTAATC
AA--CTAATC
AA--CTAATC
AA--AACAAC
AA--AAACAC
AA--CTAAAC
AA--CTAAAC
AA--CTAAAC
AA--CAAAAC
----TAAATAA--AACAAC
AA--CCCCAC
AA--CCCCAC
AA--CCCCAC
AA--CTCAAC
AA--CTCAAC
AA--AACATC
AA--CTCAAC
AA--AACACC
AA--AACAAC
AA--AACAAC
AA--AAAAAC
AA--AAAATAA--AACCAC
AA--CTCCAC
AA--CTCCAC
AA--AACAAC
AA--AACAAC
AA--AACAAC
AA--AACAAC
AA--AACATC
AA--AACCAC
AA--AAAATC
AA--CAAACT
AA--CAAACT
AA--CAAACT
AA--TAAACT
AA--CTCCAC
AA--AA-ATC
--GAGGGGG--GAGAGGG--GAGGGG---GAGGGGG--GGGGGGG--GAGGAGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GTTGGGG--GTGGGGG--GTGGGGG--GAGGGGG--GAGAGGG--GAGGGGG--GAGGGGG--GAGGKGG--GAGGGGG--GAGGTGG--GGGGGGG--GGGGGGG--GGGGGGG--GGGGGGG--GAAGGGGG
--GAAGGGG--GAGAGGG--GAGGAGG--GAGGGGG--GAGAGGG--TGGAGGG--GAGAGGG--GAGAGGG--GAGAGGG--CAGGGG-A
--GAGGGG-A
--GACAGGG--GAGATGG--GAGAGGG--GACAGGG--GAGAGGG--GAGAGGG--GAGAGGG--GAGGGGG--GAGGGGA--GAGGGGG--GAGGGGG--GAGGGGG--GAGAGGG-
TGAGG
CGCGG
TGCGG
TGCGG
TGAGG
TGCGG
TGCGG
CGCGG
CGTGG
CGCGG
TGCGG
TGCGG
TGCGG
TGCGG
CGAGG
TGCGG
TGCGG
TGCGG
AGCGG
CGCGG
CGCGG
CGCGG
CGCGG
CGCTG
TGCGG
TGAGG
CGCGG
CGCGG
CGCGG
CGCGG
CGAGG
TGCGG
TGTGG
TGCGG
CGCGG
TGCGG
TGCGG
TGCGG
GGCGG
CGCGG
GGCGG
TGCGG
TGCGG
TGCGG
TACGG
AGAGG
GGCGG
TGCGG
TGCGG
TGAGG
TACGG
CGTGG
CGCGG
CGCGG
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
-TCGAAACCT
GTCGAAACCT
GTCGAAACCT
-TCGAAACCC
-TCGAAACCT
GTCGAAACCT
GTCGAAACCT
GTCGAAACCT
GCCT-AGCAG
GCCT--GCAG
GCCT-AGCAG
GCCT--GCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT--GCAG
GCCT-AGCAG
GCCT-AGCAG
GCCC-CGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
GCCT-AGCAG
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AT--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AY--GACC
A-AC--GACC
A-AC--GACC
A-AT--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
A-AC--GACC
CGT-GAACGT
CGT-GAACAT
CGT-GAACAT
CGT-GAACAT
CGT-GAACGT
CGT-GAACGT
CGT-GAACGT
CGT-GAACGT
CGT-GAACGT
TGT-GAACGT
TGT-GAACGT
TGT-GAACRT
TGT-GAACGT
CGT-GAACGT
TGT-GAACGT
CGCGGAACGC
CGT-GAACGT
CGC-GAACGT
CGC-GAACGT
TGT-GAATGT
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GATATMAAAC
GATATCAAAC
GATATCAAAC
GATATCAAAC
GTTATCATAC
GTTATCAAAC
GTTG-CACAC
GATATAAAAC
GTTATCATAC
GTTATCATAC
GTTATCATAC
AA--CTCCAC
AA--CTCCAC
AA--CTCCAC
AA--CTCCAC
AA--CTCCAC
AA--CTACAC
AA--CTACAC
AA--CTCCAC
AA--CTCCAC
AA--CCTCAC
AA--CCCCAC
AA--CCTCAC
AA--CCCCAC
AA--AATACC
AA--AACAAC
GA--CACC-G
AA--CTCAAC
AA--AACACC
AA--AACACC
AA--AACACC
--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GAGGGGG--GGGGGGG--GGGGGGG--GAGGGGG--GAGGGGG--GGGGGGG--GGGGGGG--GGGGGGG--GGGGGGG--GAGAGGG--GAGGGGG--GGGGCGG--GAGGGGG--GAGAGGG--GAGAGGG--GAGAKGG-
TGCGG
CGCGG
CGCGG
CGCGG
TGCGG
CGCGG
CGCGG
CGCGG
CGCGG
CAYGG
TGCGG
C?CGG
YGCGG
TGCGG
AGCGG
CGCGG
CGTAA
TGCGG
TGCGG
TGCGG
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
85
ATG-CAATAT
TTG-CAATAT
ATG-CAATAT
ATG-CATCCT
ATG-CATCCT
ATG-CATCTT
ATG-CATCTT
ATG-CAATCT
ATG-CAATCC
ATG-CATCCT
GTG-CAATCT
ATG-CAATCT
GTG-CAATGT
ATG-CAATCT
GTG-CAATGT
ATG-MAATCT
ATG-CAATCT
ATG-CAATCT
ATG-CATCCT
ATG-CATCTT
ACG-CATCCT
ATG-CAATAT
ATG-CAACTT
ACG-CAATCT
ACG-CAATCT
ATG-CAATCT
ATG-CATCAT
ATG-CATCAT
ATG-CAATGT
ATG-CATCCT
ATG-CATTCT
GTG-CAGAAA
95
105
TG-CCCC--A ACCCC-TCTC
TG-TCCC--A ACCCC-TCTC
TG-CCCC--A ACCCC-TCT?
TG-CCCC--A AACCC-CCTC
TG-CCCC--A AACCC-CCTT
TG-CCCC--A AACCC-CCTC
TG-CCCC--A AACCC-CCTC
TG-CCCC--A ACCCC-TCTC
TTGCCCC--A ACCCC-TCTC
TG-CCCC--A AACCC-CCTC
TGCTCCC--A A-CCCCTCTC
TG-CCCC--A ACCCC-TCTC
TG-CCCCC-A ACCTC-TCTC
TG-CCCC--A ACCCC-TCTC
TG-CCCCC-A ATCCC-TCTC
TG-CACC--A ACCTC-CCTC
TG-CCCC--A ACTCC-TCTC
TG-CCCC--A ACCCC-TCTC
TG-CCCC--A AACCC-CCTC
-G-CCCC--A AACCC-CCTC
TG-CGCCT-A AACCC-CCTC
TG-CCCC--A AACCC-CCTC
TG-CCCC--A AACCC-CCTC
TG-CGCCG-A ATCCC-TCTC
TG-CCCC--A ATCCC-TCTC
TG-CCCC--A ACCCC-TCTC
TG-CCCC--A AACCC-TCTC
TG-CCCC--A AACCC-TCTC
TG-CCCC--A ACCCC-TCTC
TG-CACC--A AACCC-CCTC
TG-CACC--A AACCC-CCTC
TG-CCCC--A ACCTT-TCTC
115
---GATGCCT
---GATGCCT
C--GATGCCT
---GATGCCT
---GATGCCT
---GTTGCCT
---GTTGCCT
---GATGCCT
---GACGCCT
---GATGCCT
---GGTGCCT
---GGTGCCT
---GATGTCT
---GGTGCCT
---GATGTCT
---GGTGTCT
---GATGCCT
---GATGCAT
---GATGCCT
---GATGCCT
---GATGCCT
---GAACCCT
---GGTGTCA
---GATGCCT
---GATGCCT
---GATGCAT
---GATGCCT
---GATGCCT
GATGATGCCT
---GATGCCT
---GATGCCT
---GACGCCT
125
TGG---TG-T
TTGT--TGTG
TGC---TG-C
TGGCGGTGTG
TGGCGGTGTG
AGG---TGTG
AGG---TGTG
CTG---AGTG
TGG---CTGG
TGG---TGTG
TGT---TGTG
TG----TTGT
TGG---TC-T
TGG---TGTG
TG----GTGT
CGG-----TG
TGG---TTTG
TGG---TTCG
TCG---TTTG
TCG---TTTG
TGG---TGTG
CGG---TGTG
CGG---TTTG
CTG---AATG
CTG---AATG
TGG---TTCG
TGG---CGTG
TGG---CGTG
TGT---CGTG
TGG---TGTG
TGG---TGTG
CT----TTCG
135
GTTTGGTC-T
CTTTGCTC-T
GTTTGGTC-T
CTT-GGCT-C
CTT-GGCT-C
CTT-GGCC-T
CTT-GGCC-T
CTT-GGGT-C
----GGTC-T
CTT-TGCC-T
TGT-GGCT-T
GTTTGTTC-C
GTTTGGTC-T
CTT-GCCA-C
GTTTGGTC-T
CGTCGGTC-T
TTT-GGTC-T
TTT-GGTG-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GTCC-T
CTT-GGTC-T
CTT-GGTC-T
TTT-GGTG-T
CTT-GACC-A
CTT-GACC-G
CTT-GGTC-T
CTT-GGCT-CTT-GGCT-GTTCGGTC-T
145
T-GCCTC--A
T-GCATC--A
T-GCCTC--A
T-GCCCC--A
T-GCCCC--A
T-TCCCC--A
T-TCCCC--A
T-GCCTC--A
T-GCC-C--A
T-TCCCC--A
T-GCCTT--C
T-GTCTC--C
T-GCCTC--A
T-GTCTY--C
T-GCCTC--A
T-GCCTCTCA
T-GGCTG--A
A-GCCTC--A
T-TCACC--A
T-TCACC--A
T-TCACC--A
T-GCCTC--A
C-GCCTC--A
T-GGCTC--A
T-GGCTC--A
A-GCCTC--A
T-GCACA--A
T-GCACA--A
T-TGCTT--C
------------------T-GCCTC--A
150
GCCCTCCCTTCCTCCGTCCGTTCGTTCGTCCTGCATTCCGTCCTACCCACCCGCCCA
ACCCA
TCCGCTCCTCCCTCCGTCCGTCCGTCC-TCCTTCTCTCTCTCCCTCCGTTCGGCCA--------TTCC-
107
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
GTG-CAGAAA
GTG-CATCCT
ATG-CAATCT
ATGGCAATCA
ATG-CAATCT
ACG-CATCCT
ATG-CATCCT
ATG-CATCCC
AAG-CATACT
AAG-CATACT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ACG-CAATCT
ATG-CATCCG
ATG-CATCCT
ATG-CA?CCG
ACG-CAACAT
ATG-CAACTA
ATG-CATCCT
ATG-CATCCT
ATG-CATCCA
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CGTCCT
ATG-CATCCT
TTG-TATCCTTG-CATCCTTG-CATC-AAC-GAATCC
GTG-CAATCT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CAATCT
ATG-CATCTT
ATG-CATCTT
ATG-CATCTT
ATG-CA-CCT
ATG-CATCCT
GTG-CATTTG
ATG-CATCCT
ATG-CAATCT
ACG-CAATCT
GTG-CAATCT
AAC-GAATCT
ATG-CAATCT
GTG-CAATCA
ATG-CAATCT
ATG-CAATCT
ACG-CAATCT
TG-CCCC--A
CG-CCTC-TC
TG-CCCC--A
TG-CCCCC-A
TG-CCCC--A
TG-CACC-TC
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCCC-TG-CCCC--G
TG-CCCC--G
TG-CCCC--G
TG-CACC--A
TG-CGCC--G
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--G
TG-CCCC--G
TG-CCCC--G
TG-CCCC--G
TG-CCCC--G
---------A
---------A
---------A
TG-CCCC--A
TG-CCCCC-T
TG-CACCC-A
TG-CACCC-A
TG-CCCC--A
TG-CCCCC-A
TG-CACC--A
TG-CACC--G
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CACC--A
TG-CACC--A
TTGTCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCCC-TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--T
TG-CCCCC-A
TG-CCCCC-A
TG-CCCC--A
ACCTT-TCTC
GCCAC-CC-C
ACCCC-TCTC
AATCC-CCTC
AACCC-TCTC
G-TAC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
ACCCC-TCTC
AACCC-CCTC
AGCCC-CCTC
AACCC-CCTC
AACCC-CCTC
AGCCC-CCTAACCC-CCTC
AACCC-CCTM
AACTC-CCTC
AGCCC-CCTC
AACCC-CCTC
AGCCC-CCTC
AGCCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
ACCCCCTCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
ACCCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
ATCCCCTCCC
CACCC-CCTC
AACCC-TCTC
ACCCC-TCTC
ACCCT-TCCG
ATCCC-TCTC
ACCCC-TCTC
ACCCC-TCTC
AGCCC-CCTC
AACCC-CCTC
ATCCC-TCTC
108
---GACGCCT
---CGTGTCT
---GATGCAT
---GACGCCT
---GGTGCCT
---TATGCCG
---GATGTCT
---GATGTCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GTTGCCT
---GATGCCC
---GATGCCT
---GATGCCT
---GATGCCT
---GACGTCT
---GATGTCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GACGCCT
---GATGCCT
---GGTGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGTCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GTTGCCT
---GATGCCT
---GATGCCC
---TATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GACGCCT
---GACGCCC
---GATGCCT
CT----TTCG
CGG---AGCG
TGG---TTCG
TGG---TGAG
TGT---TG-T
CGG---AGTT
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
CGG---CGTG
TGG---CGTG
CGG---CGCG
TGG---TGCG
CGG---TTTG
CAG---TTTG
TGG---TGTG
TGG---ACTG
TGG---TGTG
CGG---CGCG
CGG---CGCG
CGG---CGCG
CGG---CGCG
CGG---TGCG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGC---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
AAG---TGTG
CGG-----TG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGT---TGTTGG---TGTG
TGG---TGGC
CGG---CGTG
TG----GTTT
TGG---TGTG
TGG---TGTG
TGC---TGTG
TGG---TGTG
TGG---TGTG
TGC---AGTG
GTTTGGTC-T
GCC-AGTC-T
TTT-GGTG-T
CCT-GGCT-T
GTTTGTTC-C
CTT-GGCT-T
CTT-GCCC-T
CTT-GCCC-A
TTT-GGCT-T
TTT-GGCT-T
CTT-GGCT-C
CTT-GGCT-C
CTT-GGCC-T
CTT-GGTC-C
CCC-TGCC-T
CCG-AGCC-T
CCC-GGCC-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GCCT-T
CTT-GGCT-T
CGC-AGAGCC
CCG-AGCC-T
CCA-AGCC-T
CCG-A-CC-T
CCG-AGCC-T
CCT-AGCC-T
CTT-GCCC-T
CTT-GGCC-T
CTT-GCCC-T
CTT-GACC-T
CTT-GGTC-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GGCC-T
CTT-GGCC-T
CCT-AGCC-T
CGTCGGTC-T
CTT-GCCT-T
CTT-GTCT-T
CTT-GTCT-T
CTT-GGTT-T
CTT-GGTT-T
GTTTGTTC-C
CTT-GGCT-T
GTTTGGTC-T
TTT-GG-T-G
GATTGGTC-C
TTT-GGTC-T
CTT-GGTC-T
CTT-GGTC-T
TCC-GGCT-T
CCT-GGCC-T
ATT-GGTC-T
T-GCCTC--A
CGTCGTCC-C
A-GCCTC--A
G-GCCTC--A
T-GTCTC--C
T-GCCTC--C
T-GCACC--A
T-GCACC--A
T-GTCCC--A
T-GTCCC--A
T-GCCCC--A
T-GCCC---A
T-TCCCC--A
T-GCCTC--A
T-GCCAC--A
T-GCCGC--A
T-GCCAC--A
C-GCCTC--A
T-GCCTC--A
T-GCCCC--A
T-GCCCC--A
CGGCCGC--A
T-GCCGC--A
T-GCCGC--A
T-GCCGC--A
T-GCCGC--A
C-GCCGC--A
T-GCACC--A
T-GCACC--A
T-GCACC--A
T-GCCTC--A
T-GTAGC--A
T-TCACC--A
T-TCACC--A
T-GCACC--A
T-TCCCC--A
T-GCCAC--A
T-GCTTCTCA
T-GTACC--A
T-GTACC--A
T-GTACC--A
T-GCACC--A
T-GCTCT--C
G-GTCTC--C
T-GCCCC--A
T-GCCTC--A
T-GC-TC--A
T-GCCTC--T-GGCCC--A
T-GCCCC--A
T-GCATC--A
T-GC-TC--A
T-GCCTC--A
T-GACTC--A
TCCCTTTGTTCCCCCTACCCCTTATCCGTCCGACCGACCGTCCGTCCGTTCG-CTCTACGTCCGTACG-CCC-CCCTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCCGTCTGTCTGTCCGTCCCTCTCTCCGTCCGTCCGTTCGTTCGTCCCTCCTTCCTTCCTGCCTT
GCCTT
TCC-TCCGAACC-CTCCTCCTCTCTCCCTCT-TCACCTCATGCC-
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
ACC-GAATCT
ACG-CAATCT
TTG-CAATCT
GTG-CAATAT
ATG-CAATCA
ATG-CATTCC
ATG-CATCAT
ATG-CATCAT
ATG-CATCAT
ATG-CATCAT
ACG-CATCAC
GTC-CAATCT
ATG-CATCTT
ATG-CATCYT
ATG-CATCCT
ATG-CATCCT
ATG-CATCTT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CATCCT
ATG-CAACTT
ATG-CATCTT
ATG-CATCTT
ATG-CATC-T
ACG-CAATCT
ACG-CAATCT
GCG-CATCCC
ATG-CATCAT
ATG-CAATCT
ACG-CAATCT
ACG-CAATCT
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--TG-CCCC--T
TG-CCCC--A
TG-CTCT--A
TG-CTCC--A
TG-CTCT--A
TG-CTCC--A
TG-CCCC--A
TG-CCCCC-T
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--G
TG-CCCC--G
TG-CCCC--A
TG-CCCC--A
TG-CCCC--G
TG-CCCC--A
TG-CCCC--G
TG-CCCC--A
TG-CACC--A
TG-CCCC--A
CG-CCTC-TC
TG-CACC--A
TG-CCCC--A
TG-CCCC--A
TG-CCCC--A
ATACC-TCTC
ATCTC-TCTC
ATCCC-TCCC
------TCTC
ACCCC-TCTC
ACCCC-TCTC
AACCC-CCTT
AACCC-CCTT
AACCC-CCTT
ATCCC-CCTT
AACCC-CCTC
ACCCC-TCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTT
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
AACCC-CCTC
ACCCC-TCTC
ACCCC-TCTC
GTCTC-CCTC
AACCC-CCTT
ACCCC-TCTC
ACCCC-TCTC
ACCCC-TCTC
---GATGCCT
---GCTGCCT
---GATGCCT
---GATGCTG
---GATGCCT
---GAAGCCT
A--GATGCCT
A--GATGCCT
A--GATGCCT
A--GATGCCC
---GATGCCT
---GATGCCT
---GTTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GATGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GGTGCCT
---GTAGCCT
---GATGCCT
---AGCGTCT
---GATGTCT
---GAAGCCT
---GAAGCCT
---GTAGCCT
TGG---TGCG
TGG---TGTG
TGG---TGTG
AGG---AGTG
TGC---TGTG
TGC---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---TGTG
TGG---CGTG
TGG---TGTG
TGG---TGTG
TGG---AGTG
TGG---TTTG
CGG---AGCG
TAG---TTTG
ACA---AGTG
ACA---AGTG
TGG---AGTG
GTT-GGTC-T
GTT-GGTC-T
CTT-GGTC-T
CTT-GATC-T
CTT-GGTC-T
CTT-GGTC-T
CTT-GCCC-T
CTT-GCCC-T
CTT-GCCC-T
CTT-GCCC-T
-TA-AGCT-T
CTT-GGTC-T
CTT-GCCC-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GCCT-T
CTT-GTCT-T
CTT-GTCT-T
CTT-GTCT-T
TTT-GGTC-C
CTG-GGGT-C
ATC-GGTC-C
CTT-GGCT-T
TTT-TGTT-C
TTT-TGTT-C
TTT-GGTC-C
TT-CCTC--A
T-GCCTC--A
T-GCCTC--G
T-GCGCC--C
G-GCCTC--A
T-GCCTC--A
T-ACACC--A
T-ACACC--A
T-ACACC--A
T-GCCCCC-A
T-GCCTC--A
T-GCCGC--A
T-GCCCC--A
T-GCCCC--A
T-GCCCC--A
T-GCCCC--A
T-GCCCC--A
T-GCCCC--A
T-GCCCC--A
T-ACCCC--A
T-ACCCC--A
T-GCCYC--A
T-GCCCC--A
T-GCCYC--A
T-GCCCC--A
T-GTATC--A
TTGCCTC--A
CGTCG----C-GC-----A
T-GCATC--A
T-GCATC--A
T-GTATC--A
TCTCTCTCTCTCCTCCC
TCTCTCTCTGCGTGCGTGCGTGCGTCCATCTCTCCATCCATCCATCCATCCATCCGTCCGTCCGTCCGTCCTTCCTTCCTTCCTACCCTCAT----TGC-ACCCACCCACCC-
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
160
170
ACCTC----- GTGA-GGGTG
CCCTC----- GTGA-TGGTG
ACCTT----- GTGA-GGGTG
TCCTT----- GTGC-GGGTG
TCCTT----- GTGC-GGGTG
TTCTT----- GGGC-GGGTG
TTCTT----- GGGC-GGGTG
CCCTT----- GAGA-TGATG
CCCCT----- GGGA-GGGTG
CTCTT----- GGGC-GGGTG
CCACTC---- GTGG-GGGCG
CCTC------ GTGA-GGGTG
C-TGT----- G-----GATG
TCCCTC---- GCGA-GGGCG
CC-TC----- GTGT-GGATG
ACCTT----- AGGT-TTGTG
CTCCT----- --GA-GGGTG
CGGTC----- GGGA-AGGTG
CTTTT----- GGGC-GGGTG
CTTTT----- GGGC-GGGTG
180
AGTTG-CAAA--TG-CAAAGTTG-CAAAGATA-CCAAGATA-CCAAGATT-ACAAGATT-ACAAGATG-CCACGCTG-CCAAGATG-ATAAGATG--CAAGATG-CCAAGTTG-CAAAGATG-CCAAGTTG-CAAAGAGGCCGAAGATG-CCAAGATG-CTAATAGG-TCTATAGG-TCT-
190
GTCCCA-TCC
GCTCCA-TCC
TTCCCA-TCC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTT
GGTCTT-GTT
GTTCTA-GCC
GTTCCA-GCA
GGTCTT-GTC
GTTCCA-TCC
GTTCCA-ACC
GTTCCA-TCC
GTTCCA-TCC
GTTCCA-TCC
GT-CCC-GTC
GTTCCA-TCC
CTTCCA-ACC
GGCCTT-GTC
GGCCTT-GTC
200
AC--TCCAAAC--TACAAAC--TCCAAACACTCCAAACACTCCAAGCACTC--AGCACTC--AAC--TCTGAAC--ACCGAACACTCCAAGC--TACAGAC--TCCAAAC--CCCAAAC--TCCA-AC--TCCAAAC--TCCAAAG--TCCAAGC--TCCAAG-A-TCTAGG-A-TCTAG-
210
-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAAA
-GG-CAAAAA
-GG-TAAAA-GG-TAAAA-GG-CAAAAC
-GG-CAAAAC
-GG-CAAAA-GG-CAAAAA
-GG-CAAAA-GG-CAAAAGGG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-
220
CCAA-CAA-C
TCAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
TGAA-CAA-C
TGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CAAA-CAAAC
CCAA-CAAAC
CTAA-CAA-C
CCAA-CAAAC
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
TAAA-CAA-C
225
CCCCCCC-CCCCC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-CCC-CCCCCCC-CCCCCCCCCCCCCCCCCCCCCCCC-
109
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
CTCTT----CCCTC----CCCTT----GC-TC--GAG
CC-TC--GAG
CCGTC----TTCTTT---TTCTTT---CCCTCC---------------------CCGTC----CCGTC----CCCGTC---CGGTC----CCCTC----CCTGT----CCCTCT-GTG
CTCTTT---CTCTTT---CTATTT---CTATTTT--CCCTT----CCCTT----CTGTT----CCCTC----CCCTC----TCCTC----CCCTC----CCCTC----CCCAT----CTCTC----CTCTT----CCCTC----TCCTC----TCCTC----TCCTC----TCCTC----CCCTC----CTCTTT---ACCTTT---CTCTTT---TCCTA----CCCT-----CTCTT----CTCTT----CTCTT----CTCTT----CCCTT----ACCTTT---CTCTC----CTCTC----CTCTC----CTCTT-----
GGGC-GGGTG
TGGA-GGGTG
GGGA-CGGTG
AGGG--TGTG
AGGG--TGTG
GGGA-AGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGG-GGGTG
-------------------TGGGGGGTG
-TG--GGGTG
GGGTGGGGTG
GGGA-AGGTG
GGGC-GGGTG
-TGA-GGGTG
GGGT-GGGTG
GGGC-GGGTT
GTGC-GGGTG
GGGC-GGATG
GGGC-GGATG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGT-GCGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGA-CGGTG
GGGA-CGGTG
TGGC-GGTTG
GGGC-GGGTG
TTGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGT-GGGTG
GAGA-GGATG
-GGA-TTTTG
GGGT-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-GGGTG
GGGC-AGGTG
AGGA-GGGCG
GAGC-GGTTG
GAGC-GGTTG
GAGC-GGTTG
GGGA-GGGTG
AGATG-CCAAGATG-CCA-GTGG-CCATGAT--CA-TGATA-CAAAGATG-CTAAGATG-CCCAGATG-CACAGGTA-GAA------------------AGACC-CAAAGACC-CAAAGATG-CC-G
AGATG-CTAAGATG-CC-AGATG-CCAGG-TG-CCGAGATG-CCTAGATG-CCTAGATG-CCTAGATG-CCTAGATA-CCAAGATA-CCAAGATG-ACAAGATG-CC-TGACG----G
TCCCG----CGACG----AGACG-CC-G
AGACG-CC-G
AGATG-CCAAGATG-CCAAGTTG----TCCCG----TCCTG----TCCCG----TCCCG----TGACG----AGATG-CCAAGATG-CCAAGTTG-CCAATGTG--ATAGATG-CAATGATG-GCATGATG-CCATGATG-CGAAGATG-ACATGATA----AGAGG-CGGAGATG-CAAAGATG-CAAAGATG-CAAAGATG-CAAA
110
GGTCTT-GTC
GGTCCG-GTC
GGTCTT-GTC
GTTCCA-ACC
GTTCCA-ACC
CTTCCA-ACC
GGTCAT-GTC
GGYCAT-GTC
GTTCCA-ACC
----TT-GTC
----TT-GTC
GCTCCA-TCC
GCTCCA-TCC
GGATCA-ACC
CTTCCA-ACC
GGTCT-TGCC
GTKTCA-TCC
AGCCTC-GCT
GGTCTT-GTC
GGTCTT-GTC
GGTTTA-TAC
GGTCTT-TAC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTTCA-ATC
GGTTTC-GGC
GGTTTC-GTC
GGTCTC-GTC
GGCCTT-GTC
GGCCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTTTT-GTC
GGTTTC-GTC
GGTTTC-GTC
GGTTTC-GTC
GGTTTC-GTC
GGCTTC-GTC
GGTCTC-GTC
GGTCTC-GTC
GGTCTC-GTC
GGCCTC-GTC
GTT-GA-TCC
GGTCTA-GTC
GGTCTT-GTC
GTTCTT-GTC
GGTCTT-GTC
GGTTTT-GTC
GGTCCC-GTC
G--CTT-GTC
G--CTT-GTC
G-TCTT-GTC
GGTCTT-GTC
AC--TCCAAAC--TCTGAAC--TCCGAAC--TC-GAAC--TCTGAGC--TCCAAA
ACAC-CCAAACAC-CCAAGC--TCCAAA
ACACTCCAAACACTCCAAGC--TGAGATC--TGAGATC--TTCGAGC--TCCAAA
AC--ACCAAAC--TCCAATC--TCCAAACACTCCTAACACTCCAAACACTCCAATCACTCCAAACACTCCAAACACTCCAAACACTCTTAAC--TCCGTACGCTCCAAGCGCTCCGAGCGCTCCAAAC--TCCGAAC--TCCGAACACTTCAAACACTCCAGACACT-ATAGCGCTCCGAGCGCCCCGAGCGCTCCGAGTGCTCCGAACGCTCCGAACAATCCAAAAACTCCAAACATTCCAAAC--TTCA-AC--TC-AAAC--TCCGAAC--TCCGAATACTCCAAACACTC-A-ACACTCCAAGT--TCCAA---CCCTGA---CCCTGA---CACTGAACATTCCAA-
-GG-CAAAA-GG-CAAAA-GG-CAAAA-GA-CAAAA-GA-CAAAA--G-CAAAA-GG-CAAAA-GG-CAAAAAGG-CAAAA-GG-CAAA--GG-CAAA--GG-CAAAA-GG-CAAAA-GG-CAAAG--G-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAGAAA
-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CACAA-GG-CAAAA-GG-CAAAA-GG-TCAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAAGGG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CTAAA-GG-CAAAA-GG-CAAAA-GG-CAGAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
TGAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CGAA-CAAAC
CCAA-CAA-C
CGAA-CAA-C
CCAA-CAAAC
CCAA-CAA-C
CGAA-CAA-C
CGGA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
TGAA-CAA-C
CAAA-CAAAC
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CAAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
TGAA-CAAAC
CGAA-CAAAC
CAAA-CAAAC
CGAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
TGAA-CAA-C
CGAA-CAA-C
CCAA-CAA-C
TGAA-CAA-C
TGAA-CAA-C
TGAA-CAA-C
CGAA-CAA-C
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
CTCTT----CTCTCTT--CTCTT----CACTC----CCCTC----CCCTC----CC-TCCCTAG
CC-TC--GAG
CCCAC----CTC------CCCTCA---CC-TC----CC-TC--GAG
CC-TT--GAG
CC-TC--TAG
CCATC----CCCT-----CC-TT--GAC
CTCTTTT--GTCTTTT--CTCTTTT--CTCTTT---CCCTT----CCCTA----CTCTC----CTCTC----CTCTTTTT-CTCTTTTT-CTCTC----YTCTC----CTCTT----CTCTC----CTCTC----CTCTC----CTCTC----CTCTC----CTCTT----CC-TC----CCCCT----------------------CC-TC----YC-TY----CC-TC-----
GGGA-GGGTG
GAGC---GTG
GGGC-AGGTG
ATGA-GGGTG
GGGT-GCGTG
TATA-GGGTG
AGAGGGTGTG
AGAGGGTGTA
AGGA-CGGTG
GGGC-GGGTG
GGGC-AGGGG
-GGG-GTGTG
AGGG--TGTG
AGGG----TG
AGGG--TGTG
CGGA--GGTG
AGGA-CGGTG
AGGG--CGTG
GGGC-GGGTG
GGGC-GTGTG
GGGC-GGGTG
GGGC-GGGTG
GGGT---GTG
-GGA-TTTTG
GGGC-GGTTG
GGGC-GGTTG
GGGC-GGTTG
GGGC-GGTTG
GAGC-GGTTG
GCGC-GGTTG
GGGC-GGTTG
GGGC-GGTTG
GGGC-GGTTG
GAGC-GGTTG
GAGC-GGTTG
GAGC-GGTTG
GAGC-GGTTG
GTGA-GGGTC
GGGA-GGGTG
------------------GTGA-GGATC
GTGA-GGATC
GTGA-GGGTC
TGATG-CAAA
AGATG-CCAAGATG-CCAAGATG-CCAAGATG-CC-AGATG-CTATGATA-CAATGATA-CAAAAATG-CCAAGATG-CCAAGATG-CCAAGATG-CATTGATA-AAATGATA-CAAG
TGATA-CAAAGATG-CC-G
AAATG-CCATGATA-CAAAGATG-CTTAGATG-CTTAGATG-CTTAGATG-CATAGATG-CCAAGATG-CCAAGTTG-CAAAGATG-CAAARATG-CAAAGATG-CAAAGTTG-CAAAGATG-CAAAGATG-CAAAGATG-CCAAGATG-CCAAGATG-CAAGGATG-CAAAGATG-CAAAGATG-CAAAGATG-TCAAGATG-CCA-------------G--AAAGATG-TCAAGATG-TCAAGATT-TCA-
AGTCTT-GTC
GTTCCA-TCC
GGTCAT-GTC
GTTCCA-TCC
GGTTCA-ATC
GTTCCA-GCC
GTTCCA-ACC
CTTCCA-AAC
GTTCAA-TCC
GGTCTT-GCC
GGTCTT-GCC
GTTCCA-TTC
GTTCCA-ACC
G-TCCA-ACC
GTTCCA-ACC
GTTCCC-GCC
GTTCAA-TCC
GTTCCA-AAC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GGT
GTTCGA-TCC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
TGTCTC-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
GGTCTT-GTC
CGTCTT-GTC
GGTCTT-GTC
GGCCTT-GTC
GCTCGA-TCC
GTTCCA-GCA
GGTCCG-ACC
-GTCTT-GTC
GTTCCA-TCC
GTTCCA-TCC
GTTCAA-TCC
ACATTCCAGAC--TTCAAACACTCCAAGC--TCCAAAC--TCCGTAC--TTCAAAC--CCCAAAC--CCCAAAC--TCCAAAC--TCCAAAC--TCCAACC--TTCCGA
AC--ACCAAAC--TCCAAAC--CCCAAAC--TCCGAAC--TCCAAAC--CCCAAACACTYTAAACACTCTAAACACTCTAAACACTCCA-GT--GT-ATAC--TCCAAACACTCCAAACACTCCAAACACTCCAAACACTCCAAACACTCCAAACATTCTAAACACTATAAACACTTCAAACACTTCAAACATTCCAA----TCCAAACATTCCAA----TCCAAAC--TCGAAAC--ACCGATC--TCCGGAC--TCCAAAC--TCTTAAC--TCTTAAC--TTGAA-
-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GA-CAAAA-GA-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-C-AAA-GA-CAAAA-GA-CAAAA-GA-CAAAA-GGTCAAAA-GG-CAAAA-GA-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CACAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAA-GG-CAAAAC
-GG-CGAAA-GG-CAAAAA
-GG-CAAAA-GG-CAAAA-GG-CAAGA-
CGAA-CAA-C
CTAA-CAAAC
CAAA-CAA-C
CCAA-CAA-C
CAAA-CAAAC
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAAAC
CGAA-CAA-C
CGAA-CAA-C
CCAAACAA-C
CCAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
CAAA-CAA-C
CCAA-CAA-C
CCAA-CAA-C
TAAA-CAA-C
TAAA-CAA-C
TAAA-CAA-C
TGAA-CAA-C
CGAA-CAA-C
CCAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
CAAA-CAA-C
CGAA-CAA-C
CGAA-CAA-C
TGAA-CAA-C
TGAA-CAA-C
TGAA-CAA-C
TCAA-CAA-C
CCAA-CAAAC
CGAA-CAA-C
CGGA-CAAAC
CGAA-CAA-C
CCAA-CAAAC
CCAA-CAAAC
CCAA-CAAAC
CCCCCCC-CCCCCCCCCCC-CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC-CCCCCCCCCCCCCCC-CCC-CCC--
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
235
245
GGC-GCGAAT TGCGCCAAGG
GGC-GCGAAT TGCGTCAAGG
GGC-GCGAAT TGCGCCAAGG
GGC-GCGAAT CGCGCCAAGG
GGC-GCGAAT CGCGCCAAGG
GGC-GCGAAT TGCGTCAAGG
GGC-GCGAAT TGCGTCAAGG
GGC-GTGAAT TGCGCCAAGG
255
AATT---TAA
AATT---TAA
AAAT---TAA
AAT--AAAAA
AAT--AAAAA
AATT--AAAA
AATT--AAAA
AATT--AAAA
265
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
TTG--AAAGA
275
GT-GC-ACGT
GT-GC-ACGT
GT-GC-AACG
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGA
285
-TACT-GTCG
-TACT-GTCG
TTACT-GTCT
CTGCT-GTTG
CTGCT-GTTG
CTACT-GTCA
CTACT-GTCA
-TACT-GCTG
295
CCGACCC-GT
CCGACCC-GT
CCGACCC-GT
CCGTCCC-GT
CCGTCCC-GT
TCGTCCC-GT
TCGTCCC-GT
CCTACCC-GT
300
T-TGC
T-CGC
T-CGC
G-CGC
A-CGC
A-TGC
A-TGC
T-TGC
111
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGCGGCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAC
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
CGCGCCAAGG
TGCGCCAAGG
CGTGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TACGCCAAGG
TACGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGTCAAGG
TGCGCCAAGG
CGCGTCAAGG
CGCGTCAAGG
CGCGTCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
AGCGTCAAGG
CGCGTCAAGG
CGCGTCAAGG
CGCGTCAAGG
CGCGTCAAGG
CGCGTCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
AATT---AAA
AAT--AAAAA
AAAT--TGAA
AAAT---TTA
AATT---GAA
AATT---TAA
AATT---TAA
AAAT--AAAA
AATT---AAA
AATC---AAA
AATT-ATAAA
AATT-ATAAA
AATAAAAAAA
AAT---AAAA
AAT--AAAAA
AATT---ATA
AATT----TA
AATT---AAA
AAT-ATAAAA
AAT-ATAAAA
AATT---AAA
AAT--AAAAA
AAT--AAAAA
AATT---AAA
AATT---AAA
AATC---GAA
AATT---GAA
AAC--AAGAA
AACT---TAA
AACT---ATA
AAT-AAAAAA
AAT-AAAAAA
AATAAAAAAA
AATAAAAAAA
AAT--AAAAA
AAT--AAAAA
AATT-AAAAA
AATT---GAA
AAT--AAAAT
AAT--AAGAA
AAT--AAAAA
AAT--AATAA
AAT--AAAAA
AATT-GAAAA
AATTACAAAA
AAT---AAAA
AAT--AAAAA
AAT--AAAAA
AAT--AAAAA
AAT--AAAAA
AAT--AAAAA
AATAAAAAAA
AAT-AAAAAA
AAT--AAAAA
112
ATG-AAAAGA
ATG-AAAAGA
TTG--AAAGA
ATG-AAAAGA
ATA-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ACT-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
TTG-AAAAAA
ATG-AAAGGA
ATG-AAAGGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AATAGA
ATG-AAAAGA
ATG-AAAAGA
ATA-AAAAGA
ATA-AAAAGA
ACG-AAAGAA
ATG-AAAAGA
ATG-AAAGAA
ATG-AAAAGA
ATG-AAAAGG
ATG-AAAAGA
ATTGAAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
CTG-AAAAGA
-TG-AAAAGA
ATG-AAAAGA
ATG--AAAGA
ATG-AAAAGA
ATG--AAAGA
TTG-AAAGAG
TTG-AAAAAG
ATG-AAAAGA
ATG-AAAAGA
ATG--AAAGA
ATG-AAAAGA
ATG-AAAAGA
ATT-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
AATGAAAAGA
ATG-AAAAGA
ATG-AAAAGA
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GG-GC-ACGY
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACGG
GG-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACAG
GGGGC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GC-GC-ACCT
GT-GC-ACGT
GT-GT-ATGT
GT-GC-ATGT
GT-GC-ACGT
GT-TC-ACGT
GT-TC-ACGT
GT-GC-ACGT
GG-GC-GTGT
GT-GC-ATGT
GT-GC-GTGT
GT-GC-ATGT
GG-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-GTGT
GC-GC-GTGT
GT-GC-GTGT
GT-GC-GTGT
GT-GC-GTGT
GT-GC-ACGT
GT-TC-ACGT
GT-GC-AAGT
-TACT-GTCG
CTTCT-GTCA
-CACT-GTCG
-TACT-GTCG
-CACT-GTCG
--ACT-GTCG
-CACT-GTCC
C-ACT-GATG
-CACT-CTTG
-TACT-GTTG
CTATT-ATCA
CTATT-ATCA
CTGCT-GTCG
-TGCT-GTCG
ATGCT-GTTG
-TACT-GTCG
-TACT-GTCG
-TACT-GTTG
CTGCT-GTCG
CTGCT-GTCG
-TATT-GCCG
CTGCT-GCCG
CTGCT-GCCG
-CACT-GATG
CCACT-GATG
CTTCT-GTCG
-TACT-GTTG
TCGCT-GTCG
-CACT-GTCG
CTGCT-GTCG
CTACT-GTCG
CTATT-GTCG
CTACT-GTTG
CTACT-GTTG
CTGCT-GTCG
CTGTT-GTCG
CTACT-GTCG
-TACT-GGCG
TTCGTTGTTTTCGTTGTCTTCGTTGTCG
TTGCT-GTCG
TTGTT-GTCG
TTGCT-GTCG
CTGCT-GTTG
TTGCC-GTCC
TTCGTTGTCTTCGTTGTCG
TTCGTTGTCTTCGTATCATTCGTTGTCG
TTGCT-GTCG
CTGCT-GCCA
CTGCT-GCTG
CCTACCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGACCC-GT
TCAACCC-GT
TCGACCC-GT
TCAACCC-GT
CCAACCC-GT
CCA-CCC-GT
TCAACCC-GT
CC-TACC-GT
CCGTACC-GT
CCATCCC-GT
TCGACCC-GT
ACGACCC-GT
CCATCCC-GT
CCATCCC-GT
TCAACCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGACCC-GT
TCGTCCC-GT
TCSTCCC-GT
CCAACCC-GT
CCAACCC-GT
CCGCACC-GT
TCAACCC-GT
CCGTCCC-GT
CCAACCC-GT
TCGCACT-GT
CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
TCGTCC--GT
CCAGCCC-GT
---TCCC----------GT
CAC------ACGACCC-GT
ACGACCC-GT
ACGTCCC-GT
CCGTCCC-GT
ACGTCCC-GT
--------GT
TCG--------------GT
--------GT
TCC------CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
C-CGC
A-CGC
T-CGC
T-CGC
T-CGC
T-CGC
C-CGC
C-CGC
T-CGC
T-CGC
A-TGC
A-TGT
A-CGC
C-CGC
C-CGC
C-TGC
C-TGC
T-CGC
A-CGC
A-CGC
C-CGC
T-CGC
T-CGC
A-CGT
C-CGC
T-CGC
T-TGC
A-CGC
T-CGC
T-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
C-CTG
----A-T-----C-CGC
C-CGC
A-CGC
A-CGC
G-CGC
A-T-----A-T-A-T-----C-CGC
G-TGC
T-CGC
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTTAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAA
GGC-GTGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GTGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
GGC-GCGAAT
AGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGACATCG
TGCGTCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCGAAGG
TGCGCCAAGG
CGCGCCAAGG
-GCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CACGCCAAGT
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TACGCCAAGG
TACGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
CGCGCCAAGG
CGCGCCAAGG
TGCGCCAAGG
TGCGCCAAGG
AATC---AAA
AACT--ATAA
AAT--AAAAA
AAT--AAAAA
AATAAAAAAA
AAT--AAAAA
AAT--AAAAA
AAAT--AAAA
AATT-GAAAA
AATT-GAAAA
AATT-GAAAA
AATA-ATAAA
AATA-ATAAA
AAAT---TGA
AATTAAAATA
AATC---TAA
AATT---GAA
AATT---AAA
AATT---TTA
AATT---ATA
AACT--ATAA
AAC--AAATA
AAC--AAGAA
AATT---TAA
AATT---ATA
AATT---ATA
AATT---ATA
AATT---AAA
AACT--ATAA
AATT-AT-TA
A-TT-AAAAA
A-TT-AAAAA
A-TT-AAAAA
AAT--AAAAA
AAT--AAGAA
AACT--ATAA
ATTT-GAAAA
ATTT-GAAAA
ATTT-GAAAA
ATTT-GAAAA
ATTT-TAAAA
AATT-AAAAA
AATT-AAAAA
AATT-GAAAA
AATT-GAAAA
AATT-GAAAA
AATT-GAAAA
AATT-GAAAA
AATT-GAAAA
AATT---AAA
AATT---GAA
AATC---GAA
AATT-AAAAA
AATT----AA
113
ATG-AAAAGA
-TG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG--AAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-MAAAGA
AAT-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATC-AAAGGA
ATA-AAAGGA
-TG-AAAAGA
ATG-AAAGAA
ATG-AAAGAA
ATG-AAAAGG
ATG-AAAGGA
ATG-AAAAGA
ATG-AAAGGA
ATG-AAAAGA
-TG-AAAAGA
TTT-AAAGGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-GAAAAA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
-TG-AAAAGA
ATG-AAAAGA
ATG-AAAAGA
ATT-AAAAGA
ATG-AAAAGA
AAGAAAAAGG
ATG--AAAGA
AAA-AAAAGA
GG-GC-ACGA
GG-GT-GCGA
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ATGT
GT-GC-ATGT
GG-GC-ACGT
GT-GC-ATGT
GT-GC-ATGT
GT-GC-ATGT
GT-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GA-GC-ACGT
GG-GC-GTGT
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GT-GC-GCGC
GG-GC-ACGT
GG-GC-ACGT
GT-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACGT
GG-GC-ACTT
GG-GC-ACGT
GG-GC-ACGT
GT-GC-ACAT
GT-GC-ACAT
GT-GC-ACAT
GT-AC-ATGT
GG-GCTACGT
GG-GC-ACGA
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-ACGT
GT-GC-AYGT
GT-GC-ACKT
GT-GC-ACGT
GG-GC-ACGC
GT-GC-ACGT
GGGGC-ACGT
GT-GC-ACGT
GG-GC-ACGT
-TCCC-CTCG
-TACT-GTCG
CAGCT-GTCG
CTGCT-GTCG
CTGCT-GTCA
CTGCT-ATCA
TTACTTGTCG
C-ACT-GTCG
-TGCT-GTTG
-TGCT-GTTG
-TGCT-GTTG
TTGCT-GTCG
TTGCT-GTCT
-CACT-GTCG
CTGCT-GTTG
-TACT-GTCG
-TACT-GGCG
-T-CT-GTCG
-TATT-GTTG
-TACT-GTCG
-TATT-GTCG
TTCGT-GTCG
TTCGT-GTCG
-TACT-GTCA
-TACT-GTCG
--CGT-GTCG
-TACT-GTCG
-TGCT-GTCG
-TATT-GTCG
-TACT-GTCA
CTACT-GTTG
CTAYT-GTTG
CTACT-GTTG
CTATT-GCCA
TTCGT-GTCG
-TACT-GTCG
TTGCT-GTCG
TTGCT-GTTG
TTGCT-GTCG
TTGCT-GTCG
TTGCT-GTCG
TTGCT-GTCG
TTGCT-GTCG
TTGCT-GTCA
TTGCT-GTCA
-TGY?-GTCG
-TGCT-GTTG
-TGYT-GTCG
-TGCT-GTTG
-TACT-GTCG
-TACT-GTCG
CTCCC-GTCG
CTGCT-GTCG
-TACT-CTCG
CATACCC-GT
CCGACTC-GT
CCGTCCC-GT
CCGTCCC-GT
TCGTCCC-GT
CCGTCCC-GT
C-C------CCAACCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCGTCCC-GT
CCATCCC-GT
CCATCCC-GT
TCGTCCC-GT
TCGTCCC-GT
CCGACCC-GT
CCGGCCC-GT
CCGACCC-GT
CCAACCC-GT
CCATCCC-GT
TCAACCC-GT
CCGTCCC-GT
CCGTCCC-GT
GCAACCC-GT
CCAACCC-GT
CCAACCC-GT
CCAACCC-GT
TTGACCC-GT
CCGGCCC-GT
CCATCCC-GT
CCGTCCC-GT
CCGTCCC-GT
TCRTCCC-GT
TCATCCC-GT
CCATACC-GT
CCGACCCAGT
TCGTCCC-GT
CCATCCC-GT
CCATCCC-GT
CCATCCC-GT
TCGTCCC-GT
CCGTCCC-GT
CCRTCCC-GT
ACGTCCC-GT
ACGTCCC-GT
TCGTCCC-GT
CCGTCCC-GT
TYGCCCC-GT
CCATCCC-GT
CCGACCC-GT
CCTACCC-GT
CCGCACC-GC
TCATCCC-GT
CCAACCC-GT
C-CGC
T-CGC
A-CGC
A-CGC
A-CAC
A-CGC
----C-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
T-TGC
A-CGC
T-CGC
C-CGC
T-CGC
T-CGC
C-CGC
TTCGC
A-CGC
A-CGC
C-CGC
C-CGC
T-CGC
C-TGC
T-TGC
T-CGC
C-CGC
G-CGC
T-CGC
G-CGY
A-CGC
A-CGC
T-AGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
A-CGC
T-CGC
T-CGC
C-CGC
T-CGC
T-CGC
Wissadula.cruziana
Wissadula.periplocifolia
GGC-GCGAAT TGCGCCAAGG AATT----AA AAA-AAAAGA GG-GC-ACGT -TACT-CTCG CCAACCC-GT T-CGC
GGC-GCGAAT TGCGCCAAGG AATT---AAA ATG-AAAAGA GG-GC-ACGT -TACT-GTCG CCGACCC-GT T-CGC
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
310
320
GGTGT-TCGT --GC-GGGAG
GGTGT-TTGT --GC-GGGAG
GGTGT-TTGT --GA-GGGAG
GGTGT-TTGT --GT-GACAG
GGTGT-TTGT --GT-GACAG
GGTGT-TTGT --GT-GGCAG
GGTGT-TTGT --GT-GGCAG
GGTGT-CTGT --GC-GACAG
GGTGT-GTGT --GC-GGTAG
GGTGT-CTGT --GT-GGCAG
GGTGTACTGT --GC-GGGAG
GGTGT-TTGT --GC-GGGAG
GGTGT-TTGT --GC-GGGAG
GGTGT-ATGT --GC-GGCAG
GGAGT-TTGT --GC-GGGAG
GGTGT-GCGT --GC-RTCAG
GGTGT-TTGT --GC-GGGAG
GGTGT-TTGT --GC-GGCAG
GGTGT-MTGT --GT-GGTAT
GGTGT-CTGT --GT-GGTAT
GGTGT-CTGT --GT-GGCAG
GGTGT-TTGT --GC-GGCAG
GGTGA-TCGT --GC-GGCAC
GGTGT-ATGT --GA-GGCGG
GGTGT-ATGT --GA-GGCGG
GGTGT-TTGT --GC-GGCAG
GGTGT-ATGT --GT-GGCAT
GGTGT-ATGT --GT-GGCAT
GGTGT-TTGT --GC-GGTGG
GGTGT-CTGT --GC-GGTAG
GGTGT-CTGT --GC-GGTAG
GGTGT-TTGT --GC-TGTAG
GGTGT-TTGT --GC-AGTAG
GGTGT-CGAT --GC-TTCAG
GGTGT-TTGC --GC-GGCAG
GGTGT-ACGT --GC-AGCAG
GGTGT-TTGT --GC-GGGAG
GGTGT-CCGT --GC-GACAG
GGTGT-CTGT --GT-GGTAG
GGCGT-CTGT --GT-GGCAG
GGTGT-CTGT --GT-GGCAG
GGTGT-CTGT --GT-GGCAG
GGTGT-CTGT --GT-GGCAG
GGTGT-CTGT --GT-GGCAG
GGTGT-CTGT --GT-GGCAG
CTTAA-CTGT CCGTCGGCAG
---------- -----GGCAG
---------- -----GGCAG
---------- -----GGCAG
GGTGA-TCGT --GC-GACGC
330
TTTC---GCT
TGTC---GCT
TGTC---GCT
CGAT---GTT
CGAT---GTT
TGAT---GCT
TGAC---GCT
TATC---GTT
TATC---GTT
TGAC---GTT
CGTC---GCT
TGTC---GCT
TGTC---GTT
TGTC---GCT
TGTC---GCT
TGTC---RCT
TGTC---GCT
TGTC---GAT
TGAT---GCT
TGAT---GCT
TGAC---GCT
TGTC---GTT
AGAC---GAT
TGAC---GCT
TGAC---GCT
TGTC---GAT
AGAT---GTT
AGAC---GTT
TGAC---GTT
TGAC---GTT
TGAC---GTT
TGAC---GCT
TGAC---GCT
TGAT---GTT
TGTC---GAT
TGGC---GCT
TGTC---GTT
AGGG---GTG
AGAC---GCT
TGGC---GCT
TGAT---GTT
TGAT---GTT
CGAC---GCT
CGAC---GTT
TGAC---GCT
TGAC---GCC
CGAG---GAT
CGAG---GGC
CGAG---GAT
TGAC---GAT
114
340
G-CTACTTTT
G-CTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CCACTTTT
G-CCACTTCT
G-TTACTTTT
G-CTTCTTTT
G-CTTCCTCT
G-ACACTT-T
G-CTTCTTTT
G-TTACTTTT
G-CTRCTTTT
G-CC-CTTCT
G-CTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CAACTTTT
G-CTACTTTT
G-CTACCTTT
G-CTACCTTT
G-CTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CCGCTTTT
G-CCACTTTT
G-TT-CTCTT
G-CTACTTTT
G-CTACTCTT
G-CTTCTTTT
G-CTGCCTTG-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
GCC-ACTTCT
G-TTACTTTT
G-TCGCTCTC
G-TCACTTTT
GCC-ACTTTT
350
GTCGTGAAAGTCGTGAATGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAATGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTTAAAGTCGCGAAAGTCGCGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTCAAAGTCGTTATAGTCGTTAAAGTCGCAAAAT
GTCGTGAAAGTCGTGAAAGTCGTGAAAGTTGTGAAAGTCGTAAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAA
GTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTTAAT-
360
A-TACATCGT
A-TAC---GT
A-TACATCGT
A-TACATCGT
A-TACATCGT
--TACATCGT
A-TAC---GT
A-TA---TGT
ATACG----T
A-TA---CGT
A-TAC---GT
A-TATATCGT
A-TAAATCGT
A-TATATCGT
A-TACATCGT
A-TACATCGT
A-AACATCGT
A-TACATCGT
A-TATATTGT
A-TATATTGT
A-TACATCGT
A-TAC---GT
A-TACATCGT
ACAC----GT
A-------GT
A-TACATCGT
A-TACATTGT
A-TACATTGT
A-TACATCGT
C-TACATCGT
C-TACATCGT
A-TACATCGT
A-TAC---GT
A-TAC---GT
A-TACATCGT
A-TACATCGT
A-TATATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TTTATTGT
A-TTTATTGT
A-TACATCGT
A-TACATCGT
--T--A-CGT
A-TAC---GT
A-TA---CGT
A-TAC---GT
A-TAAATCGT
A-TACATCGT
370
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCCT-A
CGCCCC-T-A
CGCCCCA--A
CGCCCCA--A
CGCCCCC--C
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCA--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCA--A
TGCCCAC--A
TGCCCAC--A
CGCCCCC--A
CGCCCCCCAG
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCA--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCCA-A
CGCCCCCA-A
CGTCCCC--T
CGCCCCC--T
CGCCCCC--A
CGCCCCA--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCC--A
CGCCCC---A
CGCCCC---A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGTCCAC--A
TGCCCCC--A
CGCCCCC--G
TGCCCCC--A
CGCCCCCC-A
375
TCAAA
CCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAT
CCAAT
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAG
TCCAT
TCCAT
TCAAA
TTAAA
TTAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCCAA
TCAAA
TCAAA
TCTTA
CCCAA
TTAAA
TTAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
--AAA
TCAAA
TCAAA
TCAAT
TCAAT
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
GGTGA-TCGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
---------------------------------------------GGTGT-CTGT
GGTGT-CTGT
GGTGT-ATGC
GGTGT-CTAT
GGTGT-TTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CCGC
GGTGT-CTGT
---------GGTGT-ACGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
GGGTT-TTGT
GGGTT-TTGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-TTGT
GGTGT-AAGT
GGTGT-TTGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-TTGT
GGTGT-ACGT
GGTGT-ACGT
GGTGT-TTGT
GGTGT-ATGT
GGTGT-GTGT
GGTGT-ATGT
GGTGT-CAGT
GGTGT-TTGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-TTGT
GGTGC-TCGT
GGTGT-TTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
--TT-GACAG
--GC-GGCAG
--GT-GGCAG
--GT-GGCGG
-----GGCAG
-----GGCAA
-----GGCAG
-----GGCAG
-----GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGCAG
--GC-GATGG
--GC-GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGCAG
-----GGCAG
--GC-GGCAG
--GC-GACAG
--GC-GACAG
--GC-GACAG
--GA-GGCAG
--GA-GGCAG
--GC-GGGAG
--GT-GGCAG
--GC-GAGAG
--CG-GGCAG
--GC-GGCAG
--GC-AGCAG
--TA-GGCAG
--TC-GACAG
--GC-AGCAG
--GC-AGCAG
--GA-GGCAG
--GT-GGCAG
--GA-GGCAG
--GA-GGCAG
--TC-GGCAG
--GC-GGCAG
--GA-GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGTAG
--GT-GACAG
--GC-GGCAG
--GC-GGCAG
--GT-GGCAG
--GT-GGCAG
--GT-GGCAG
--GC-GGCAG
--GC-GGCAG
CGAC---GGT
TGAC---GTT
TGAC---GCT
CGAC---GGT
CGAG---GGC
CAAA---CAC
CGAG---GGC
CGAG---GGC
CGAG---GAT
TGAC---GCT
TGAC---GCT
TGAC---GTT
TGTC---GTT
TGTTCTAGTT
TGAC---GCT
TGGC---GCT
TGAC---GTT
TGAC---GCT
TGAG---GAT
CGTC---GCT
TGAT----TT
TGAT----TT
TGAT----TT
TGAC---GTT
TGAT---GTT
TGTC---GCT
TGAC---GCT
TGTC---GCT
TGAC---GCC
TGTC---GCT
TGAC---GTT
TGAC---GAT
TGTC---GCT
CGGC---GCT
TGGC---GCT
TGAC---GCT
TGGC---GTT
TGAC---GCT
TGGC---GCT
TGAT---GCT
TGTC---GCT
TGAC---GTT
TGAT---GTT
TGAT---GTT
TGAT---GTT
TGAT---GTT
TGAC---GTT
TGTC--AGTT
TGAC---GTT
TGAC---GTT
TGAC---GTT
TGAC---GTT
TGAC---GTT
TGAT---GTT
115
G-CTACTTTT
----ACTTTT
G-TTACTTTT
G-TTACTTCT
G-TCGCTCTC
G-TCACTCTC
G-TCACTCTC
G-TCACTCTC
G-TCACTCTA
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TCACTTTT
GCC-ACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CTTCTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CTACTTTT
G-TTACTTTT
G-CTTCTTCT
G-TTACTTTT
G-CTACTTTT
GCC-ACTTCT
G-CTACTTTT
G-CTACCTTT
G-CTACCTTT
GCTTTCTTTT
G-TTACTTTT
G-CTACTCTT
G-CTACTTTT
G-CTACCTTT
G-CTACTTTT
G-CTACCTTT
G-CCACCTTT
G-CTACTTTT
G-CTACCTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-ATACTTTT
G-CTACTTTT
GCC-ACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
GTCGTTAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAA
GTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTAAAAGT-GTAAAAGTCGTAAATGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTKAAAGTCGTGAAAGTCGTTAAAGTCGTGAAAGTCGTGAAAGTCGTGAATGTCGTGAATGTCGTGAATGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAATGTCGTCAAAGTCGTGAATGTTGTTAAAGACGTGAATGTCGTGAATGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTTAAAA
GTCGTAAATGTCGTGAAAGTCGTGATAGTCGTGATAGTCGTGATAGTCGTGAAAGTCGTGAAA-
A-TACATTGT
A-TACATCGT
A-TACATCGT
A-TAA--CGT
A-TAAATCGT
A-TAAATCGT
A-TTAATCGT
A-TTC---GT
A-TAAATCGT
A-TACATCGT
A-TACATCGT
A-TAC---GT
A-TAC---GT
A-TA---TGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TAC---GT
A-TAC---GT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
G-TATATCGT
A-TACATCGT
GATA----CG
A-TAC----T
T-TATATCGT
A-TA---CGT
A-TA---CGT
A-TAC----T
A-TAC---GT
A-TAC---GT
T-AA---CGT
A-TA---CGT
A-TA---CGT
A-TA---CGT
A-AAC---GT
A-TT---CGT
A-AA---CGT
A-TACATCGT
A-TACATTGT
A-TACATCGT
A-TACATTGT
A-TAC---GT
A-TAT---GT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCC--G
CGCCCCC--G
CGCCCCC--G
CGCCCCC--G
CGCCCCC--G
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCAT--A
TCGCCCC--A
CGTC-CC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCA--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCAC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TGCCCCC--A
CGCCCCC--C
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CCAAT
TCAAA
TCAAT
CCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCATA
TCAAA
TCAAA
TCAAT
CTAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCAAA
TCTAC
CAAAA
TCAGA
TCAAA
TCAAA
CCAAA
TCAAA
TCAAA
TCTAA
TCAAA
TCAAA
TCAAA
TCAAA
CCAAA
TCAAG
ATAAA
ATAAA
ATAAA
ATAAA
TCAAA
CTAAA
TCAAA
TCAAG
TCAAG
TCAAG
TCAAA
TCAAA
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
GGTGT-CTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-YTGT
GGTGT-CTGT
GGTGT-CTGT
GGTGT-TTGT
GGTGT-ATGT
GGTGT-GTGT
GGTGC-CGGT
GGTGT-CTGT
GGTGT-ATGT
GGTGT-ATGT
GGTGT-ATGT
--GC-GGCAG
--GT-GGCAG
--GT-GGCAG
--RC-GACAG
--GC-GACAG
--GC-GACAG
--GC-GACAG
--GC-GGGAG
--GC-GGTAG
--GC-TTCGG
--GC-GGCAG
--GC-GGGAG
--GC-GGGAG
--GC-GGTAG
CGAT---GTT
TGAC-----TGAC---GTT
TGAT---GTT
TGAT---GTT
TGAT---GTT
TGAT---GTT
TGTC---GCT
TACC---GTT
GGAC---GAC
CAAC---GCT
TGTC---GCT
TGTC---GCT
TGTC---GCT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-TTACTTTT
G-WTACTTTT
G-TTACTTTT
G-TTACTTTT
G-CTTCTCTT
G-CTACTTCT
G-TC-CTCCG
G-TTACTTTT
G-CTTCTTTT
G-CTTCTTTT
G-CTTCTTTT
GTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTTAAAGTCGTGAAAGTCGCAAAAT
GTCGTGAAAGTCGTGAAAGTCGTGAAAGTCGTGAAA-
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATTGT
A-TACATCGT
A-TACATCGT
--TACATCGT
AATACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
A-TACATCGT
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--C
CGCCCCCA-A
CGCCCCC--A
CGCCCCC--A
CGCCCCC--A
TCAAA
TCAAA
TCCAA
TCAAC
TCAAA
TCAAC
TCAAA
TCAAA
CCAAA
TCCAA
TCAAA
TCAAA
TCAAA
TCAAA
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
385
395
CCTCGAG-T- --TTA--TTC
CCTCGAG-C- ---TA--TTCCTCGAG-C- --TTA--TTC
CCCTGAG-C- ---CC--TCG
CCCTGAG-C- ---CC--TCG
CCCTAAG-C- ---CA--TCG
CCCTAAG-C- ---CC--TCG
CTCTGAG-T- ---CT-ATTG
CCCCGTG-T- ---CT--TAC
CTCTGAG-C- ---CC--TTG
CC-CGAG-C- --AAT--TTA
CC-CGAG-C- --TCT--TCC
CCTCGAG-C- ---TT-ACCC
CCCGAGCTC- ------TTTC
CCTCGAG-C- --TTA--CTC
CCCCGAG-C- ---CT--TCG
TCTCGAG-C- ---TC-TTAT
CCTCGAG-T- ---CT--TTC
CCTTTAG-C- ---CC--TCG
CCTTTAG-C- ---CC--TCG
CCCTTGG-C- ---CC--TCG
CCCTGAG-C- ---CC-TCGG
CCCTAAG-C- ---CC--CAG
CCTCAAG-C- ---CT--TTT
CCTCAAG-C- ---CT--TTT
CCTCGAG-T- ---CT--TTC
ACCTAAGAC- ---CC--TCG
ACCTAAGAC- ---CC--TCG
CCTTGAG-C- ---CC-TTGG
TC-TCAG-C- ---CC--TCC
TC-TCAG-C- ---CC--TCC
CCTCGAG-C- ---CT--CTC
CCTCGAG-C- ---CT--CTC
CCATGAG-C- ---CCTCGAG
CCTCGAG-T- ---CT--TTC
CCCCGAG-C- ---CA-C-GG
CC-CGAG-C- --TGT--TTY
CCTGTAG-C- ---CCTCGTG
405
AGC-TCAGGT
GGC-TAAGGT
GGC-TCAGGT
AGC-TACGGT
GGC-TACGGT
CGC-TACGGT
CGC-TACGGT
GC--TAAGGT
AGC-ACAACG
GGC-TACGGT
TGC-TCGGGA
GGT-TCAGGT
GGC-TCAGGT
GGC-TCAGGT
GGC-TCAGGT
GGC-TCAGTT
GGC-TCAGGT
GGC-TCAGTT
GGT-TATGGA
GGT-TATGGA
GGC-TACGGT
G---TCAGGG
AGC-TAAGGT
GGC-AAATGA
GGC-AAATGA
GGC-TCAGTT
GTC-TAAGGT
GTC-TAAGGT
GCA--CAGGT
GGC-TGCG-T
GGC-TGCG-T
GGC-TCAGGT
GGC-TCAGGT
C---CTCGGT
GGC-TCAGTT
GC--TACGGG
GGC-TCGACA
A---TACGGT
415
-CAAAT--TG
-CAA-T--TG
-CAAAT--TG
-TGAATATTG
-TGAATATTG
-TGCAT--TG
-TGCAT--TG
-CAATT--TG
TTGTAT--TG
-TGCAC--TG
-CGTTT--TG
-CAATT--TG
-CAGTTT-TG
-CAATA--TG
-CAGTT--TG
-TGGAT--TG
-CAAAT--TG
-T-TAT--TG
-TGAAT--TG
-CAAAC--TG
-TGAAC--TG
-TGAACG-TG
-TGAAG--TG
-GGAAG--TG
-GGAAG--TG
-T-TAT--TG
-TATAG--TG
-TATAG--TG
-CGAAG--TG
-TGAAC--TG
-TGAAC--TG
-GAAAC--TG
-GAAAC--TG
-TGGAC--CG
-T-TAT--TG
GTGGAG--TG
-CGATT--TG
-TGAGG--TG
425
AGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGAG-C-GGA
TGGG-C-GGA
TGAG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-CATGA
TGGG-T-GGA
TGGG-C-GGA
TGGG-C-GGA
TGAG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
CGGG-C-GGA
TGAG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-T-GGA
435
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGGCCTC
AACTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
GAATGGCTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGCCTTC
AATTGGCTTC
GAATGGCTTC
AACTGGCCTC
AACTGGCCTC
AATTGGCTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGGCCTC
GAATGGCTTC
AATTGGCTTC
AATTGGCCTC
AATTGGCTTC
445
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCTTGTGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCCTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGCGCGCTC
CCGTGTGYTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGAGCTM
CCGTGTGCTT
450
ATCGC
ACCGT
ACCGT
ACCGC
ACCGC
ACCGC
ACCGC
ACTGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGT
ACCGC
ACCGT
ACTGC
ACCGC
ACCGC
ATCGC
ATCGC
ACCGC
A-CGC
ACCGC
ATCGC
GCCGC
ACCGC
ATCGT
ATCGT
ACCGC
ACCGC
ACCGC
ATCGC
ATCGC
ACAGC
ACCGC
ACCGC
ACCGC
GTTGC
116
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
CCCTAAG-CCCCTAAG-CCATTGAG-CCACTGAG-CCCCTGAG-CCCCTGAG-CCCCTGAGC-CCTCGAG-CCCACGAG-CCCCCGAG-CCCTCGAG-CCCCTGAG-CCCTTGAG-CACCTAAG-CCCCTGAG-CCCATAAC-CCCCCGAG-CCCCCGAG-CCCCCGAG-CCCCCGAG-CCCCCGAG-CCCCTAAG-CCCCTAAG-CCCCTAAG-CC--TTAC-CCCTCGAG-CCCCTGAG-CCCCTTAT-CCCCTGAG-CCCCTGAG-CCCCTGAG-CCCCCGAG-CCCATTAT-CCCATTAG-CCCATTAG-CCCCTCAG-CCCCTCAG-CCCCT-AG-CCCTTAAG-CC
CGTCGAG-CCCTCGAG-CCCTCGAG-CCCTCAAG-CCCTCAAA-CCCTCTAG-CCTCCAAG-CCCCCAAG-CCCCTGGG-CCCTCAAG-CCCTCAAG-CC--------CCTCGAA-CCCTCGAG-CCCTCAAG-C-
---CC--TCG
---CC--TCG
---CC--TTG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TTG
---CT--AAG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TAG
---CC--CAG
---CC--TTG
---CC--TCG
---CA--TCG
---CC--TCG
---CC--TAG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TTG
---CC--TCG
---CC--TCG
---CT-AAGG
---CT--AAA
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TTG
---CT--TTC
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TAG
---CC--TCG
--AAT--TTC
TAACC--TTG
--TCA--TTG
---CT--AAG
---CT--GTG
---CT--TTG
---CT--TTG
---CT--AAA
---CA-CG----CA-CT-G
---CC-TT-C
---CT--TTG
---CT--TTG
------------CT-TTT---CT--AAA
---CT--TTG
GGC-TATGGT
GGC-TACGGT
GGC-TACGGT
GGC-TACGGT
GGC-TACGGT
GGC-TACGGT
GCG-TACGGT
TGC-TCGGGT
GGC-CAAGAT
GGC-CGGGAT
GGC-CACGAT
GGC-CAAGGT
GGC-CAAGGC
GGC-TACGGT
GGC-TACGGT
GGC-TATGGT
GGC-CGGGAT
GGC-CGGGAT
GGC-CGGGAT
GGC-CGGGAT
GGC-CGGGAT
GGC-TACGGT
GGC-TACGGT
GCG-TACGGT
GT--TTAGTT
AGC-TCGAGA
GGC-TACGGT
GGC-TACGGT
GGC-AACGGT
GG--TACGGA
GGC-TACGGT
GGC-CGGGTG
GGC-AACGGT
GGC-AACGGT
GGC-AACGGT
GGC-TACGGT
GGC-TACGGT
TGC-TCAGGG
GGC-TACGGT
GGC-TCAGGT
TGC-TCGGGT
GGC-TCAGGT
GGC-AAATGT
GGA-AAAGGT
AGC-TCGGGT
GG-CTATGGG
GC--TATGGG
GGC-TCAGAT
TGC-GAAGGT
GGC-AAAGAG
--C-TCAG-T
GGC-TCAGTG
AGC-TCGGGT
GGC-AAAGGT
117
-TGAAT--TG
-TGAAC--TG
-TGTAA--TG
-TGTAA--TC
-TGAAC--TG
-TGAAC--TA
-TGCAC--TG
-CGAAG--CG
-TGATG--TG
-CGACG--CG
-TGATG--CG
-TGAAG--CG
-CGAAG--TG
-TGAAT--TG
-TGAAW--TG
-TGAAG--TG
-CGACG--CG
-TGACG--CG
-CGACG--CG
-CGACG--CG
-TGATG--CG
-TGAAT--TG
-TGAAT--TG
-TGAAT--TG
-TGTAC--TG
-TGAAC--TG
-TGAAC--TG
-TGAAC--TG
-TGAAT--TG
-TGAAC--TG
-TGATG--TG
-TGAAT--CG
-TTAAC--TG
-TTAAC--TG
-TTAAY--TG
-TGAAT--TG
-TGAAT--TG
-CTATC--TG
-TTAAC---G
-CAATT--TG
-CGAAG--CG
-CGAAA--TG
-GGAAG--TG
-GGAAA--TG
-TGAAA--TG
-TGGAG--TG
ATGGAG--TG
-AGAAG--TG
-GAAAG--TG
-GGAAG--TG
-GAAATG-TG
-TTAAA--TG
-TGAAA--TG
-GGAAG--TG
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
CGGG-C-GGA
CGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
CGGG-C-GGA
CGGG-C-GGA
CGGG-C-GGA
CGGG-C-GGA
CGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGGGC-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
AGGG-C-GGA
TGGG-C-GGA
TGTGGC-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TTGG-C-GGA
AACTGGCCTC
AACTGGCCAC
AAATGGCCTC
AATTGTCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AACTGGCTTC
AATTGACCTC
AATTGGCCTC
AATTGGCCTC
AACTGGCTTC
AATTGGCTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AACTGGCCTC
AACTGGCCTC
AATTGGCTTC
AATTGGTTTC
AACTGGCCTC
AACTGGCCTC
AACTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGTCTC
AATTGGTCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCCTC
AATTGGCTTC
AACTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
AACTGGCTTC
AATTGGTTTC
AATTGGCTTC
AATTGGCTTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTT
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTA
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCAC
CCGTGCGCAC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGAGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGAGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGATC
CCGTGTGCTC
CCGTGAGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGAGCTC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACTGC
ACCGC
ACCGC
ACCGC
ACCGC
AACGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ATCGC
ATCGC
ACCGC
ATATC
ACCGC
AC-GC
ACC-ACCGA-CGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCCACCGC
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
TCCTAAG-CTCCTAAG-CTCCTAAG-CCTC--------------CCCT-GAG-CCCATTAG-TCCATTAG-TCCATTAG-TCCATTAG-TCCATTAG-TCCATTAG-CCCATTAG-CCCCTAAG-CCCCTWAG-CCCATTAG-CCCATTAG-CCCATTAG-CCCATTAG-CCCC-GAG-CCCCCGGG-TCCC------CCCTCAG-T-CCTGAG-C-CCTGAG-CACC-GAG-C-
---CC--TAG
---CC--TAG
---CC--TAG
------------CC--TCG
---CT--AAA
---CC--TCG
---CT--TCG
---CT--TYG
---CT--TCG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TTG
---CC--TTG
---CC--TCG
---CC--TCG
---CC--TCG
---CC--TCG
---TC-TTTT
---CT--TAC
------------CC--TCG
---TC-TTTG
---TC-TTTR
---TC-TTTT
GGC-TATGGT
GGC-TATTGT
GGC-TATGGT
----TAAGGT
GG--TACGGG
AGC-TGAGAT
GGC-TACGGT
GGC-TATGGT
GGC-TATGGT
GGC-TATGGT
GGC-TATGGT
TGC-TACGGT
TGC-TATGGT
GGC-TACGGT
GGC-TATGGT
GGC-AAYAGT
GGC-AACGGT
GGC-AACAGT
GGC-AACGGT
GGC-TAAGGT
GGC-TCAGGT
------CGGT
GGC-TGCGGT
TAC-TTAGGT
TRC-TTAGGT
TGC-TCAGGT
-TTAGT--TG
-TTAGT--TG
-TTAGT--TG
-TTAAC--TG
-TGTAG--TG
-GGAAC--TG
-TGAAC--TC
-TGAAC--TG
-TGAAC--TG
-TGAAC--TG
-TGAAC--TG
-TGAAT--TG
-TGATT--TG
-TGAAT--TG
-TGAAT--TG
-TGAAC--TG
-TGARC--TG
-TGAAC--TG
-TGAAY--TG
-CATTT--GG
-TGAAG--TG
-TGGAC--TG
-TGAAC--TG
-CATTT--TG
-CATTT--TG
-CATTA--TG
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
AGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
YGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
CGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
TGGG-C-GGA
AATTGGTCTC
AATTGGCCTC
AATTGGTCTC
AATTGGCCTC
AATTGGCTTC
AATTGGTTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGGCCTC
AATTGGCCTC
AATTGGCTTC
AATTGGCTTC
AATTGGCTTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGTGCTC
CCGTGCGCTC
CCGTGYGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGCGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
CCGTGCGCTC
ACCGC
ACCGC
ACCGC
ATCGC
A-CGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
ACCGC
GGTGC
ACCGC
AAGGC
AMGGC
ACCGC
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
460
470
GT-GCGGTTG GCCT-AAA-A
GC-TCGGTTG GCCT-AAA-A
GC-GCGGCTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAAATC-GCGGTTG GCCT-AAAATC-ATGGTTG GCCT-AAAATC-ATGGTTG GCCT-AAAACT-GCGGTTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAAATC-GCG-TTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAA-A
GC-ACGGTTA GCCT-AAA-A
TC-GCGGTTG GCCT-AAA-A
GC-CCGGTTA GCCT-AAA-A
TT-GCGGTTG GCCT-AAAATC-GCGGTTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAA-TC-GCGGTTG GCCT-AAA-TC-GCGGTTG GCCT-AAAATC-GCGGTTG GCCT-AAA-A
TC-GCGGTTG GCCT-AAAATC-GCGGTTG GCCC-AAA-TC-GCG-TTG GCCC-AAA--
480
AT-GAGTCTT
AT-GAGTCTT
AT-GAGTTTT
TA-GAGTCCT
TA-GAGTCCT
TTTG-GTCCT
TTTG-GTCCT
AT-GAGTCCT
AT-GAGTCCT
TT-GAGTCCT
TC-GAGTCTT
AC-GAGTCTT
AT-GTGTCTT
AY-GAGTCTA
AT-GAGTCTT
AC-GAGTCCT
AT-GAGTCAT
AT-GAGTCTT
TTCGGATCCT
TTTGGGTCCT
TT-GAGTCCT
TC-GAGTCCT
TC-GGGTCTT
TATAAGTCCT
TATAAGTCCT
490
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATTA
C-GGCGATTA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
CCGGCGATGA
C-GGCGATGA
C-GGCGATTA
C-GGCAATTT
C-GGCGATGA
C-GGCGATTT
C-GGCGATGA
C-GGCGAACA
C-GGCGATAA
C-GGCGATGA
T-GGCGATGT
?-GGCGATGT
C-GGCGATGA
C-GGCGATGA
C-GGCTATGA
C-GGCGATAA
C-GGCGAT-A
500
AG-TGCC-GC
AG-TGCG-AC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AT-TGCC-GC
AT-TGCC-GC
AG-TGCC-GC
AC-TGCC-GC
AG-TGCC-GC
CG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCT-GC
AG-TGCC-AC
AG-TGCC-GC
AG-TGTC-GC
AG-TGTC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-CGCC-GC
CG-TGCC-GC
AG-TGCC-GC
510
GACAA-TCGG
GACAA-TCGG
AACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-ACGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAAATCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
520
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAAATGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
525
TT--T
TT--T
TT--T
TT--T
TT--T
TTAAT
TTAAT
TT--A
TA--T
TT--T
AT--C
AT--T
TT--T
AT--C
TT--T
TC--T
TT--G
TT--C
TT--T
TT--T
TT--T
TT--T
TT--T
TC--T
TC--T
118
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
CA-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TT-GCGGTTG
TA-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TT-GCGGTTG
TT-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
ACACGCGGTT
T--GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGCTG
TC-GCGGTTG
TTCGCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-ACGGTTG
TC-GCG-TTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TT-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
CC-GCGGTTG
TC-GTGGTTG
TC-GCGGTTG
TC-GCG-TTG
GCCT-AAA-A
GCCT-AAAAGCCT-AAAAGCCT-AAA-GCCT-AAAAGCCT-AAAAGCCT-AAA-A
GCCT-AAA-A
GCCT-AAA-T
GCCT-AAA-A
GCAT-AAA-A
GCCT-AAA-G
GCCT-AAA-A
GCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGGCCAAAAAGCCTTAAAAGTCT-AAA-GTCT-AAA-GTCT-AAA-GCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAA-GTCT-AAA-GTCT-AAA-GTCC-AAA-GTCT-AAA-GTCT-AAA-GCCT-AAAAGCCT-AAA-GCCT-AAAAGCCT-AAA-A
GCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGTCT-AAA-GCCT-AAAGGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCC-AAA-A
GCCT-AAAAGCCT-AAA-A
GCCT-AAAA-
AT-GAGTCTT
TT-GAGTCCT
TT-GAGTCCT
TACGAGTCCT
TT-GAGTCCT
TT-GAGTCCT
AT-GAGTCTT
TT-GAGTCTT
TC-GAGTCCT
AT-GAGTCTT
TC-GAGTCAT
AT-GAGTCTT
TC-GAGTCTT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TCTGAGCGAT
AT-GAGTCTT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TC-GAGTCCT
TC-GAGTCCT
TT-GAGTCCT
TC-GAGTCCT
TCTGAGTCCT
TTCTAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TT-GAGTCCT
TTTGAGTCCT
TC-GAGTCCT
TT-GAATCCT
AT-GAGTCTT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TTTGAGTCCT
AT-GAGTCCT
TC-GAGTCCT
TC-GAGTCTT
TM-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
AT-GAGTCTA
TT-GATTCCT
AT-GAGTCTT
AT-GAGTCTT
119
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATCA
C-GGCGATCA
C-GACGACAT
C-GGCGATGA
C-GGCGATAA
T-GGCGATTT
C-GACGATGG
C-GGCGATGA
C-GACGATGA
C-TGTAATGA
C-GGTAATGA
C-GGCGATGA
C-GGCGATGA
T-GGATGACG
C-GGCGATGA
C-GGCGATGA
C-GGCGATGG
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCTATGA
A-GGCGATGA
C-GGCGATGG
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATAA
C-GGCGA-AA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
T-GGCGA--C-GGCGAACA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
T-GGCGATTA
C-AGCGATGA
C-GGCGATGA
C-GGCGATGA
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
CA-TCGTCGC
AG-TGCC-GC
TG-TGCC-GC
AG-TGCC-GC
TA-ACATCGC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
GG-TGCC-GC
AAGTGCC-GC
AG-TGCC-GC
---TCCC-GC
AG-CGCC-GC
AG-TGCC-GC
AG-CGCC-GC
AG-TGCC-GC
AG-CGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-AC
AGGCGCC-GC
AG-CGCC-GC
AG-CGCC-GC
AG-------AG-CGCC-GC
AG-CGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-AA
CG-TGCC-GC
AG-TGCC-GC
AG-TGTC-GC
AG-TGCC-GC
AG-TGCC-GC
AT-TGCC-GC
AG-TGCC-GC
AG-TGYC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-CGCC-GC
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGC
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGC-TCGG
GACAA-TCGG
GACAA-CCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGG
GACGA-TCGG
---------GACGA-TCGG
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GGCAA-TCGG
GACAA-TCGG
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGTAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGTAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGTGAA--TGGGAA-CGC
TGGGAA-TGC
TGGGAA-CGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAT-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-CGC
TGGGAA-CGC
---------TGGGAA-CGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-CGC
TT--C
TT--C
TT--C
TC--T
TT--T
TT--T
TT--T
TT--T
TG--C
TT--C
TG--T
AT--T
CT--A
TT--T
TT--T
ATT-T
TTT-T
TT--T
TT--T
TT--C
TC--C
CT--T
CT--T
TT--T
TT--T
TG--T
TT--T
AT--T
TC--T
CT--T
CT--T
----CT--T
TT--T
TT--T
TA--T
TT--T
TT--A
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TC--T
TT--T
TT--T
TT--T
TT--T
TT--T
AT--C
TT--A
TT--T
TC--C
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
TC-GCGGTTG
TT-GCG-TTG
TC-GCCGTTG
TC-GCGGTTG
TC-GCG-TTG
TC-GCGGTTG
TC-GTGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCG-TTG
TC-GCG-TTG
TC-GCGGTTG
TC-GCG-TTG
TC-GCGGTTG
TC-GYGGTTG
TC-GCGGTTG
TC-GTGGTTG
TC-GCGGTTG
TC---GGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-GCGGTTG
TC-ACGGTTG
TC-GCGGTTG
TC-ACGGTTG
TC-GCGGTTG
TT-GCGGTTG
TC-GCGGTTG
TA-CCGGTTG
TC-GCGGTTG
TC-GCGGTTT
TC-GCGGTTT
TC-GCGGTTG
GCCT-AAA-A
GCCC-AAA-GCCC-AAA-GCCT-AAAAGCAT-AAA-A
GCAT-AAA-A
GCCT-AAA-T
GCCC-AAA-GCCC-AAA-GCCC-AAA-GCCT-AAA-A
GCCT-AAAAGCCC-AAA-GCCT-AAAAGCTT-AAAAGCCT-AAAAGCTT-AAAAGCCT-AAA-GCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAAAGCCT-AAA-A
GCCT-AAA-A
GCCT-AAA-T
GCCT-AAAAGCCT-AAA-A
GCCT-AAA-A
GCCT-AAA-A
AT-GAGTCCT
TATAAGACCT
TATAAGTCCT
AA-GAGTCCT
TC-GAGTCAT
TC-GAGTCAT
AT-GAGTCCT
TATAAGTCCT
TATAAGTCCT
TATAAGTC-T
-T-GAGTCCT
AA-GAGTCTT
TGTAAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TTTGAGTCTT
AT-GAGTCTT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TT-GAGTCCT
TC-GAGTCCT
TC-GAGTCCT
TC-GAGTCCT
TC-GAGTCCT
AC-GAGTCTT
AT-GAGTCCT
TC-GAGTCCT
TT-GAGTCCT
AC-GAGTCTT
AC-GAGTCTT
AC-GAGTCTT
C-GGCGATGA
C-GGCGATAA
C-GGCGATGA
C-GGCGATAA
C-GGCGATAA
C-GGCGATAA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATTA
C-GGCGATAA
C-GGCTGAGA
C-GGCGATGA
C-GGCGTTGA
C-GGCGATGA
T-GGCGATGA
C-GGCGATGA
C-GGCGA-AA
C-GGCGATGA
C-GGCGAKKA
C-GGCGATCA
C-GGCGATSA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGATGA
C-GGCGAATA
C-GGCGATGA
C-GAAGACGG
C-GGCGATGA
C-GGCGAATA
C-GGYGAATA
C-GGCGAATA
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGTC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
TG-TGCC-GC
AT-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
GG-TGCC-TC
CG-TGCC-GC
AG-TGCC-GC
AG-TGCC-RC
AG-TGCY-GC
AG-TGCC-GC
AG-TGCC-GC
AG-CGCC-GC
AG-TGCC-AC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
AG-TGCC-GC
CA-TCGTCAC
AG-TGCC-GC
AG-TGCC-AC
AG-TGCC-AC
AG-TGCC-GC
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACGA-TCGG
GACGA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
GACAA-TCGG
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-CGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-CGC
TGGGAA-TGC
TGGGAA-CGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAT-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAT-TGC
TGGGAT-TGC
TGGGAA-TGC
TGGGAT-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGTGA-CGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TGGGAA-TGC
TT--T
TT--T
TT--T
TT--T
TG--T
TG--T
TT--T
TT--T
TA--T
TT--T
TT--C
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--T
TT--A
TT--T
TT--T
TT--T
TT--C
TT--T
TT--T
TC--T
TT--T
CATCC
TT--T
TT--T
TT--T
TT--T
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
535
545
CAGCT-GCCT CG-TTCGAAG
CTGCT-GTCT CG-TTCGAAG
CAGCT-GCCT CG-TTCGAAG
TAGCT-GCCT CG-TTCGTAG
TAGCT-GCCT CG-TTCGTAG
AAGCT-GCCT CG-TTAGTTA
AAGCT-GCCT CG-TTAGTTA
TGGCT-GCCT C-TTTCGAAG
CAGCT-GCCT CG-TTCGAAG
TAGCT-GCCT CG-TTCGAAG
AAGCA-GCCT CG-TTCGAAG
GAGCT-GCCT CG-TTCGAAG
TTGCT-GCCT CG-TTCGAAG
555
TCGTGTGC-G
TCGTGTGC-G
TTGTGTGT-G
TCGTGTGC-G
TCGTGTGCTG
TCGTGTGT-G
TCGTGTGT-G
TTGTGTGC-A
TCGTGTGC-G
TCGTGCGC-G
TCGCGTGT-G
TCGCGTGT-G
TCGTGTGT-G
565
CTCGTCGATT
CTCGTCGATT
CTCGTCGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCGATT
CTCGTCGATT
CTCGTTGATT
CTCGTCGAGT
CTCGTCGAAT
CTCGTCGATT
575
---TGGA-CC
---TTGA-CC
---CGAA-CC
---AGGA-CC
---AGGA-CC
---TGGA-CC
---TGGA-CC
---CTGA-TC
---CGGA-TC
---AGGA-CT
---TGGA-CC
---CGGA-CC
---CTGA-CC
585
-CTAT-GACC
-CTAT-GACC
-CTAT-GACC
-CTCT-GACC
-CTCT-GACC
-CTTTTGACC
-CTTTTGACC
TCT---GACC
-TTAT-GACC
GTTCT-GACC
-CCGT-GACC
-CTAT-GACC
-CTAT-GACC
595
CTTTT-GGCA
CTCTT-GGCA
CTTTT-GGCA
CTTTTTGGCA
CTCTTTGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTCGTGCA
CTTTTTGGCA
CTTATT-GCA
CTCTT-TGCA
CATTT-GGCA
600
TCACA
TCACA
TCACA
TCGCA
TCGCA
TCACT
TCACT
TCGCA
TCGCA
TCGCA
TCGCA
TCACA
TCACA
120
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
CAGCT-GCCT
TTGCT-GCCT
CAGCT-GCCT
TAGCT-GCCT
AAGCT-GCCT
TAGCA-GCCT
TAG?A-GCCT
TGGCA-ACCT
CAGCT-GCCT
TAGCT-GYCT
CAGCT-GTCT
CAGCT-GTCT
AAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TGGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
AAGCA-ACCT
AAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
GAGCT-TCCA
TTGCT-GCCT
TTGCT-GCCT
AAGCT-GCCT
AAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
AAACT-GCCT
AAGCT-GCCT
CGGCT-GCCT
TGGCT-GCCT
TGGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TGGCT-GCCT
TGGCT-GCCT
---------TGGCT-GCCT
TGGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
AAGCA-GCCT
CGGCT-GCCT
TAGCA-GCCT
TAGCA-GCCT
TAGCA-GCCT
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGTAG
CG-TTCGAAG
CG-TTTGTAG
CG-TTTGTAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGTAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGGTG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TCA--TA
CG-TTCGAAG
CG-TACGGAG
CG-TTCGGAG
CG-TACGGAG
CG-TTCGTAG
CG-TTCGTAG
CG-TACGAAG
CG-TACGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
---------CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGTAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
TCGCGTGT-G
TCGTGTGT-G
TCGTGAGT-G
TCGTGTGC-G
TCGTGTGC-G
TCGTGCTT-G
TCGTGTTT-G
TCGTGTGC-G
TCGTGTGC-T
TCGTGTGT-G
TCGTGGGT-A
TCGTGGGT-A
TCGYGTGC-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGC-G
TCGTGTGC-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGCGC-G
TCGTGTGC-G
TCGTGTGC-G
TCGCGTGT-G
TCGCATGT-G
TCGTGTGC-G
TCGTGTGT-G
TTGTGTGC-A
TTGTGTGC-A
TCGTGTGC-G
TCGTGTGC-G
TCGTCGG--TCGCGTGC-G
TCGCGTGC-C
TCGCGCGC-G
TCGCGCGC-G
TCGTGTGC-G
TCGTGTGC-G
TCGTGTGT-G
TCGTGTGC-G
TCGCGTGT-G
TCGCGCGC-G
TCGCGCGC-G
TCGCGCGC-G
TCGCGCGC-G
TCGCGCGC-G
TCGTGTGC-A
TCGTGTGC-G
TCGCGTGC-T
TCTTGTGC-A
TCGTGTGC-G
TCGTGTGT-G
TCGTGTGC-G
TCGTGTGC-G
121
CTCGTCGATT
CTCGTCGATT
CTCGCTGATT
CTCGACGATT
TTCGTCGATT
CACGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCGATT
CTTGTTGATCTCGTCGATT
CTCGTCGATT
TTCGTCGATC
CTCGTTGATT
CTCGTTGATT
CTCGTCGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCAAAT
CTCGTCCAAT
TCCGTCGATC
TTCGTCGATT
CTCGTCGATT
CTCGTCGAAT
CTCGTTGGTG
CTCGTTGATT
CTCGTTGATT
CTCGTTTATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
-TCGTTCCGG
CTCGTCGATT
CTCGTTGATC
TTCGTCGATC
CTCGTTGATC
CTCGTCGATT
CTCGTCGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
TTCGTCGATC
CTCGTCGATC
CTCGTCGATC
CTCGTCGATC
CTCGTCGATC
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
---TGGA-CC
---TTGA-CA
---TGGA-CC
---AGGA-CC
---AGAA-CA
---AGGA-TC
---AGGA-TC
---AGGA-CC
---TGGA-CC
---CGGA-CC
---CGGA-TC
---CGGA-TC
---AGAA-CA
---AGGA-CC
---AGGA-CC
---CGGA-TC
---AGGA--T
---AGGA--T
---AAGA-CA
---AGGA-CA
---GAGA-GA
---AGAA-CA
---TGGA-CC
---TGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
TCCAAAAGCT
---TGGA-CC
---GGGA-CG
---GGGA-CG
---GGGA-AG
---CGGA-CC
---CGGA-CC
---AGGA-CC
---AGGA-CA
---GGGA-AC
---GGGA-CG
---GGGA-CG
---GGGA-CG
---GGGA-CG
---GGGA-CG
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-TC
---TAGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
-CTCA-GACC
-CTAT-GACC
-CTAT-GACC
-TTAT-GACC
-TCAT-GACC
-CTCT-GACC
-CTTT-GACC
-CTCT-GACC
-CTAT-GACC
-CTCT-GACC
-TTGT-GACC
-TTGT-GACC
-TTAT-GACC
-GTCT-TACC
-CTCT-TACC
-TTAT-TACC
-CTCT-GACC
-CTCT-GACC
-TTAT-GACC
-TTAT-GACC
-TCGGTGGTA
-TCAT-GACC
-CTTT-GACC
-CYAT-GACC
-TTG--GACC
-CTCT-GACC
-CTCT-GACC
-TGTT-GACC
-TGCT-GACC
-CTCT-GACC
-CTCT-GACC
CTCGATGACC
-CTTACGACC
-CTCTTGACC
-CTTTCGACC
-CTCTTGACC
-TTCA-GACC
-TTTT-GACC
-CTCT-GACC
-CTCT-GAAC
-CTTCTGACC
-CTTTCGACC
-CTTTCAACC
-CTTTCGACC
-CTTTCGACC
-CTTTCGACC
-CTCT-GACC
-CTTT-GACC
-CTGT-GACC
-CTAT-GACC
-CCAT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
CTTTT-TGCA
CTTTT-GGCA
C-TGTTGGCA
CTTCT-GGCA
CTTTT-TGCA
CTTTT-GGCA
?TTTT-GGCA
CTTTTTGGCA
CTTTT-GGCA
CTTAT-GGCA
CTT----GCA
CTTTT--GCA
CCTTTTTGCA
CTTTT-GGCA
CTTTT-GGCA
CCATTTCGCA
CTTTTTGGCA
CTTTTTGGCA
CTGTT-AGCA
CTGTT-AGCA
A--TGCTGCA
CTTTTT-GCA
CTTCTT-GCA
CTCTT-TGCA
CTTTT-GGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTCTGGCT
CTTTCTGGCT
CTTTTTGGCA
CTTTTTGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTCTCGGCA
CTTTAAGGCA
TTTC-CGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTTTGGCA
CTTTTTGGCA
CTCTTTGGCA
CTTTAAGGCA
CTTTAAGGCA
CCTTAAGGCA
CCTTAAGGCA
CTTTTAGGCA
CTTCTTGGCA
CTTCTTGGCA
CTTCTTGGCA
CTTTT-GGCA
CTTTCTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
TCGCA
TCACA
TCGCA
TCACA
TCACA
TCATA
TCATA
CCGCA
TCGCA
TCGCT
TCGCC
TCGCC
TCACA
CCGAA
CCGAA
TTGCA
TCGCA
TCGCA
TCTAA
TCAAA
A-GCA
TCACA
CCGCA
TCGCA
TCGCA
TCGCA
TTGCA
TCGCA
TCGCA
TTGCA
TCGCA
TCGCA
TCGCA
TCGCA
TCGCG
TCGCA
CCGCA
TCGCA
TCACA
TCGCA
TTGCA
TCGCG
TCGTG
TCGCG
TCGCG
TCGCG
TCACA
TCACA
TCACA
TCAAA
TCACA
CCGCA
CCGCA
TCGCA
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
TAGCA-GCCT
TAGCT-GCCT
TA?CT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
CAGCT-GCCT
TAGCT-GCCT
CAGCT-GCCT
GAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
CAGCT-GCCT
TAGCT-GCCT
CAGTT-GCCT
TAGCT-GCCT
CAGCT-GCCT
TAGCT-GCCT
CAGCT-GCCT
GAGCT-GCCT
TAGCT-GTCT
TAGCT-GTCT
TAGCT-GTCT
TAGCT-GTCT
ATGCT-GCCT
CGGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TAGCT-GCCT
TTGCT-GCCT
TAGCT-GCCT
ATCGGCCGCC
TAGCT-GCCT
CTGCT-GTCT
CTGCT-GTCT
TTGCT-GCCT
610
CG-TTCGTAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGTAG
CG-TTCGGAG
CG-TTTGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGTAG
C-TTTCGAAG
C-TTTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGTAG
C-TCTCGGAG
CG-TTCGAAG
C-TTTCGAAG
CG-TTCGAAG
CG-TGCGAAG
C-TTTCGAAG
CT-TTCGGAG
CT-TTCGGAG
CT-TTCGGAG
CG-TTCGGAG
CG-TTGGAAG
CG-TTCGAAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTTGTAG
CG-TTCGGAG
CG-TTTGTAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGAAG
TCGTTCGGAG
CG-TTCGGAG
CG-TTCGAAG
CG-TTCGAAG
CG-TTCGAAG
TCGTGTGC-G
TCGCGTGT-G
TCGTGAGC-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGC-G
TCGTGTGC-G
TCGCGTGT-G
TCGTGTGC-A
TCGTGTGC-G
TCGCGTGC-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGC-G
TCGTGTGC-G
TCGTGTGC-G
TCGCGCAC-G
TCGTGCGT-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGTGC-G
TCGCGTGT-A
TCGTGTGC-T
TCGTGTGC-T
TCGTGTGC-T
TCGTGTGC-T
TCGTGCGC-G
TCGTGTGC-G
TCGTGTGC-A
TCGTGTKC-A
TCGTGTYC-A
TCGTGTTC-A
TCGTGTGC-A
TCGTGTGC-G
TCGTGTTC-G
TCGTGTGT-G
TCGTGTGT-G
TCGTGYRT-G
TCGTGTGT-G
TCGTGYGT-G
TCGTGTGT-G
TCGTGCGT-G
TCGTGTGC-G
TCGTGCAC-G
TCGTGTGC-T
TCGTGTGT-G
TCGTGTGT-G
TTGTGCGT-G
620
122
CTCGTTGATT
CTCGTTGATT
CTCGTCGATC
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCGAAT
CTCGTTGATT
CTCGTCGAAT
CTCGTCGATT
CTCGTCGATT
CTCGTCGAAT
CTCGTCGATT
CTCGTCGATT
CTCGTCGATT
CTCGTCGATC
CTCGTCGATT
CTCGTCGATT
CTCGTTGATT
CTCGTCGATT
CTCGTCGGTT
CTCGTCGATT
CTCGTCGATT
CTCGTTGATW
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTCGATT
CTCGTTGATT
CTCGTCGATT
CTMGYTGATT
CTCGTYGATT
CTCGYTGATT
CTCGTCGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGATT
CTCGTTGAAT
CTCGTCGAAT
CTCGTCGATT
TTCGTCGATC
CTCGTTGATT
CTCGTCGAAT
CTCGTCGAAT
CTCGTCGAAT
---AGGA-TC
---GGGA-CA
G--GGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---TGGA-CC
---AGGA-CC
---TTGA-CC
---TGGA-CC
---AAGA-CC
---CGGA-TC
---CGGA-TC
---TGGA-CC
---TGGA-CC
---TGGA-CC
---CGGA-TC
---CGGA-TT
---CGGA-TC
---CGGA-TC
----GGA-CC
---CCGA-CC
---CGGA-TG
---AGGA-CC
---AGGA-CC
---AGGA-MC
---AGGA-CC
---CGG--CC
---TAGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CA
---AGGA-CA
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---AGGA-CC
---TGGA-CC
---CGGA-TC
---GGGA-CC
---AGGA--C
---TGGA-CC
---TGGA-CC
---CGGA-CC
-CTCT-GACC
-CTTTTGACC
-CTTT-GACC
-CTTT-GACC
-CTTT-GACC
-CTTT-GACC
-CTTT-GACC
-CTTT-GACC
-CTAA-GACC
-CTCT-GACC
-CTAT-GACC
-CT-ACGACC
-CTAT-GACC
-TTAT-GACC
-TTAT-GACC
-CTAT-GACC
-CTTATGACC
-CTTT-GACC
-CT-ACGACC
-TTAT-GACC
-TTAT-GACC
-TTAT-GACC
-CCAT-GACC
-CT-ACGACC
-TTAT-GACC
-CTTTTGACC
-CTTT-GACC
-CTTTTGACC
-CTCT-GACC
-CTTT-GACC
-CCAT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTCT-GACC
-CTYT-GACC
-CTCT-GACC
-CTCT-GACC
-CTTT-GACC
-CCAT-GACC
-TTAT-GACC
-TC---GACC
-CTCT-GACC
-CTAT-GACC
-CTAT-GACC
-CTA--GACC
CTTTTTGGCA
CTTTT---CA
CTATC-GGCA
CTTTTTGGCA
CTTTTTGGSA
CTTTTTGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTTT-GCA
CTTTTTGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTC-GGCA
CTTTT-GGCA
CTTCT-GGCA
CTTTT-GCAC
CTTGTT-GCA
CTTTAT-GCA
-TAAT-GGCA
TTT-C-GCGA
CTTTT-GGCA
CTTTT-GGCA
CTTTC-GGCA
CTTTA-GGCA
CTTTT-GGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTATGGCA
CTTTTTGGCA
CTTTC-GGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTTTGGCA
CTTTT-GGCA
CTTTTTGGCA
CTTTT-GGCA
CTTTTTGGCA
CTTTT-CGCA
CTCTTT-GCA
CTCTC-GGTA
CTTTTTGGCA
CTTTT-GGCA
CTTTT-GGCA
CTTTT-GGCG
CCGCA
TCGCA
TCG-TCACA
TCACA
TCCCA
TCGCA
TCGCA
ACGCA
TCGCA
TCGCA
TCGCA
TTGCA
TCGTA
TCGCA
GCACA
TCGCA
TCGCA
TCGCA
TCGCA
TCGCA
TCGCA
TCGCA
TCACA
TCGCA
TCGCA
TCGCA
TCGCA
TCACA
TCGAA
TCACA
TTGCA
TCTCA
TCTCA
TCTCA
TTGCA
TCGCA
TCGCA
TCGCA
TCGCA
TCACA
TCACT
TCACA
TCACA
TCGCA
TCGCA
CCGCG
TCGCA
-CACA
-CACA
TCACA
Abutilon.andrewsianum
Abutilon.pubistamineum
Abutilon.sp.
Acaulimalva.alismatifolia
Acaulimalva.dryadifolia
Alcea.rosea
Alcea.rugosa
Allosidastrum.pyramidatum
Allowissadula.holosericea
Anisodontea.capensis
Anoda.crenatiflora
Bakeridesia.gloriosa
Bastardia.bivalvis
Bastardiastrum.cinctum
Bastardiopsis.densiflora
Batesimalva.violacea
Billieturnera.helleri
Briquetia.sonorae
Callirhoe.digitata
Callirhoe.involucrata
Calyculogygas.uruguayensis
Corynabutilon.vitifolium
Cristaria.andicola
Dendrosida.breedlovei
Dendrosida.wingfieldi
Dirhamphis sp.
Eremalche.exilis
Eremalche.parryi
Fryxellia.pygmaea
Fuertesimalva.jacens
Fuertesimalva.limensis
Gaya.atiquipana
Gaya.calyptrata
Gossypium.hirsutum
Hochreutinera.amplexifolia
Hoheria sp.
Horsfordia.exsita
Howittia.trilocularis
Iliamna.bakeri2
Iliamna.latibrac.2B
Iliamna.remota
Iliamna.rivularis
Kearnemalvastrum.lacteum
Kearnemalvastrum.sp
Kitaibela.vitifolia
Krapovickasia.physaloides
Lavatera.arborea
Lavatera.occidentalis
Lavatera.trimestris
Lecanophora.chubutensis
Lecanophora.heterophylla
Malacothamnus.chilensis
Malacothamnus.fasciculatus
Malope sp.
A-TGTTGG-A-TGATGG-A-TGTTGG-A-CGTCGA-A-CGTCGA-A-TGTCGA-A-TGTCGA-A-TGACGG---TGACGG-A-AGTCGA-A-CGTCGG-A-CGTTGG-A-TGTTGG-A-CGTTGA-A-TGTTGG-A-TGTTGG-G-TGTTGG-A-TGTTGT-A-TGTTGA-A-TTTTGT-A-CGTCGA-A-TGTCGG-C-TGTCGG-A-TGCCGG-A-TGCCGG-A-TGGTGT-A-TGTCGA-A-TGTCGA-A-TAACGG-A-CGTCGA-A-CRTCGA-C-CATTGG-C-AATTGG-A-CCTCGT-A-TGTTGT-A-CGTCGG-A-TGTCGG-A-GGACGG-A-CGTAG--A-CGTCG--A-TGTCG--A-TGTCG--A-CGTCGA-A-C------A-TGTCGA-A-CGTCGTGG
A-CGTCGA-A-CGTCGA-A-CGTCGA-A-CGTCGG-A-CRTCGG-A-TGTTGA-A-TGTCGA-A-CGTCGA--
TGCTC-GCATGCT--GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCCA-GCGTGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-RCATGCCC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCT--GCATGCTCTGCATGCCA-GCATGCCA-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGC----CATCGGAGTCGT
TGCTC-GCATGATC-GCATGCTC-GCATGCTC-GCA------------------------------------TGCTC-GCA---------TCCTCAACGTGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCTTGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCA-
TCG
TCG
--TCG
TCG
TCG
TCG
--TCG
TCG
CTC
TCG
TCG
TCG
TCG
TCG
TCG
ATG
T-TCG
TCG
TCG
TCG
TCG
TCG
ATG
TCG
T-TCG
TCG
TCG
TCG
TCG
GCG
ATG
TCG
TCG
TCG
--------TCG
----TCG
TCG
TCG
ACG
TCG
TCG
TCG
TCG
TCG
123
Malva.assurgentea
Malva.dendromorpha
Malva.linnei
Malva.nicaensis
Malva.wigandii
Malvastrum.amblyphyllum
Malvastrum.americanum
Malvastrum.coromandelianum
Malvella.sagittifolia
Meximalva.filipes
Modiola.caroliniana
Modiolastrum.lateritium
Monteiroa.glomerata
Napaea.dioica
Navaea.phoenicea
Neobrittonia.acerifolia
Nototriche.anthemidifolia
Nototriche.flabellata
Nototriche.pedicularifolia
Palaua.camanensis
Palaua.rhombifolia
Periptera.punicea
Phymosia.umbellata
Pseudabutilon.umbellatum
Rhynchosida.physocalyx
Robinsonella sp.
Sida.abutifolia
Sida.aggregata
Sida.fibulifera
Sida.hermaphrodita
Sida.hookeriana
Sida.jussieana
Sida.linifolia
Sida.longipes
Sida.odorata
Sida.oligandra
Sida.platycalyx
Sida.xantii
Sidalcea.cusickii
Sidalcea.hirsuta
Sidalcea.multifida
Sidalcea.stipularis
Sidasodes.colombiana
Sidastrum.paniculatum
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.philippiana
Sphaeralcea.wrightii
Tarasa.albertii
Tarasa.humilis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.operculata
A-CGTCGA-A-CATCGA-A-CGTCGA-A-CGTCGA-A-CGTCGA-A-CGTCGA-A-CGTCGA-A-CATCGA-T-ATATGG-A-TGTTGG-A-AGTCGA-A-CGTCGA-A-CGTCGA-A-CGTCGA-A-TGTGCA----------A-AGTAGA-A-AGTAGA-A-AGTAGA-T-TGTCGA-A-CGTCGG-A-TGTTCG-A-TGTTG--A-TGTCGG-A-CGTCGTGG
A-TGTCGA-A-TTACGG-G-TGACGG-A-CGGTGG-A-CGTCCG-A-CGTCGG-A-TGACGG-A-TGACGA-A-CGACGG-A-TGACGG-A-TGACGG-A-TGATGG-A-TGACGG-T-TGTTGA-T-TGTTGA-T-TGTTGA-A-TGTTGA-A-CTTTGG-A-TGTTGG-A-CGTCAA-A-TGTCGA-A-TGTCGA-A-TGTCGA-A-CGTCAA-A-CGTCGA-A-CGTCGA-A-TGTTGA-A-TGTTGA-A-CGTAGA--
TGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATCGTC-GCATGCTC-GCATGCTC-GCATGCT--GCATGC---GCATGC----CATGCTC-GCATGCTC-GCATGCTC-GCATGCT--GCATGCGCT--A---------TGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCA---------TGCT--GCATGCTC-GCATGCTC-GCATGC?---CATGCT--GCATGCT--GCAGTAAT-GCATGCT--GCATGC?C---ATGC?---CATGC?---CATGCTC-GCAA
TGCT--GCATGCT--GCATGCT--GCATGCTT-GCATGCTT-GCATGCTG-GCATGCTT-GCATGC----CATGC----CATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCWTGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCA-
TCG
TCG
TGC
TCG
TAC
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TC--TCG
TCG
TCG
TCG
TCG
TCG
--TCG
TCG
TCG
TCG
TCG
--TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
--T-T-T-TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
124
Tarasa.rhombifolia
Tarasa.thyrsoidea
Tarasa.trisecta
Tetrasida.chachapoyensis
Tetrasida.weberbaueri
Thespesia.lampas
Urocarpidium.albiflorum
Wissadula.boliviana
Wissadula.cruziana
Wissadula.periplocifolia
A-AGTCGA-A-CGTAGA-A-WGTCGA-AATGTTGA---TGACGG-A-GGACGG-A-CGTCGA-A-TGTTGG-A-TGTTGG-AATGGTGA--
TGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCATGCTC-GCT-
TCG
TTG
TCG
TCG
TCG
--TCG
TCG
TCG
TCG
125
Appendix B. Tarasa and outgroups combined chloroplast data matrix
matK-3’trnK (1-779), psbA-trnH (780-1437), trnT-trnL (1438-3291)
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
10
T----CCTTC
-TAATCCTTC
TAAATCCTTC
----TCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
???ATCCTTC
----TCCTTC
TAAATCCTTC
----TCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
T----CCTTC
TAAATCCTTC
TAAATCCTTC
TAGTTACTTC
-------TTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
TAAATCCTTC
----TCCTTC
TAAATCCTTC
T--------TAAATCCTTC
TAAATCCTTC
---------C
TAAATCCTTC
T----CCTTC
20
AGTGGTACGG
AGTGGT-CGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AG?GG???GG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
-------CGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
AGTGGTACGG
30
GGTCAAATGC
GGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
GGTCAAATGC
GGTCAAATGC
GGTCAAATGC
GGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
AGTCAAATGC
40
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
TAGAAAATTC
126
50
ATTTATAATA
ATTTATAATA
ATTTATAATA
ATTTATAATA
ATTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
ATTTATAATA
ATTTATAATA
ATTTATAATA
ATTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
TTTTATAATA
60
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
GATAATGCTA
70
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGT?
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
TGAAGACGTT
80
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACGAGA
GGATACGAGA
GGATACGAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACGAGA
GGATACAAGA
GGATACGAGA
GGATACGAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
GGATACAAGA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
90
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATAA
ATTCCA?TAA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
ATTCCAATTA
100
110
120
TTTCTCTTAT TGGATCATTG TCTAAGGAAA
TTTCTCTTAT TGGATCATTG TCTAAGGAAA
TTTCTCTTAT TGCATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
TTTCTCTTAT TGGATCATTG TCTAAGGCAA
130
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
AATTTTGTAA
140
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGA
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
CACATTAGGG
150
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCTATTA
CATCCTATTA
CATCCTATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
CATCCCATTA
160
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAT
GTAAGCCGAT
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCTAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
GTAAGCCGAC
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
170
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
180
190
200
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
TCCTCCGATT CTGATATTAT TGACCGATTT
210
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
220
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
230
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
240
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
127
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
GTGGGCCGAT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
TCCTCCGATT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
CTGATATTAT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
TGACCGATTT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GTGCGTATAT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GTAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GTAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
GCAGAAATCT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
TTCTCATTAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
CACAGTGGAT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
250
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
260
270
280
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAAAGTTTG TATCGAATAA AATATATACT
AAAAAGTTTG TATCGAATAA AATATATACT
AAA-AGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
AAAGAGTTTG TATCGAATAA AATATATACT
290
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
300
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
310
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
320
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
128
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAA?A
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
CTTCAAAAAA
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
AAAGAGTTTG
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
TATCGAATAA
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
AATATATACT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TCGGTTTTCT
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
TGTGTTAAAA
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
CTTTGGCCCG
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
TAAACACAAA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
330
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
340
350
360
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
GTGCTTTTTT GAAAAGATTA GGTTCGGAAT
370
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
380
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACA
390
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
400
AAAACCATGT
AAGACCATGT
AAGATCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
129
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
AGTACTGTAC
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GTGCTTTTTT
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GAAAAGATTA
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GATTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
GGTTCGGAAT
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
TTTTGGAAGA
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACA
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
ATTCTTTACG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
GAAACGGAAG
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
AAGACCATGT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
410
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
420
430
440
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTTCCAA GAGGGTTTTT TGCTTTGCGA
ATCTTTCCAA GAGGGTTTTT TGCTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTTCAAA AAGGTTTTTT TACTTTGCGA
ATCTTTCAAA AAGGTTTTTT TACTTTGCGA
ATCTTTCAAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA GAGGTTTTTT TGCTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
ATCTTCCCAA AAGGTTTTTT TACTTTGCGA
450
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
460
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
470
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
480
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
130
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
TTTTTCTTTG
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
ATCTTCCCAA
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
AAGGTTTTTT
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
TACTTTGCGA
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
AAGTTCTATC
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
GGGGACGAAT
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
TTGGTATTTG
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
GATATTATTT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
490
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GGATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
GTATCAATGC
500
510
TCTGGTCAAT CATGAATGAT
TCTGGTCAAT CATGAATGAT
TCTGGTCAAT CATGAATGAT
TCTGGTCAAT CATGAATGAT
TCTGGTCAAT CATGAATGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGAATGAT
TCTGGTCAAG CATGAATGAT
TCTGGTCAAG CATGAATGAT
TCTGGTCAAG CATGAATGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
TCTGGTCAAT CATGACTGAT
520
TGGTTATAAA
TGGTTATAAA
TGGTTATAAA
TGGTTATAAA
TGGTTATAAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATAAA
TGGTTATAAA
TGGTTATAAA
TGGTTATAAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
TGGTTATGAA
530
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
ATTATGTAAA
540
TTCAATTTAA
TTCAATTTAA
TTCAATTAAA
TTCAATTAAA
TTCCATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
TTCAATTAAA
550
ATAATAA--ATAATAA--ATAATAA--ATAATAAGAA
ATAATAAGAA
ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA--ATAATAA---
560
--AATAAAAA
--AATAAAAA
--AATCAAAA
AAAAAAAAAAAAAAAAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATAAAAA
--AATAAAAA
--AATAAAAA
--AATAAAAA
--AATAAAAA
--AATAAAAA
--AATAAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATAAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATAAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
--AATCAAAA
131
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
570
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
TGAGAATTTT
580
590
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TTCGAAATGA TCAAGAGATA
TTCGAAATGA TGAAGAGATA
TTCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCGGAAATGA TGAAGAGATA
TCGGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGA?ATA
TCCGAAATGA TGAAGAGATA
TCCGAAATGA TGAAGAAATA
TCCGAAATGA TGAAGAGATA
600
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGCAT
ACAAACGCAT
ACAAACGCAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAATGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGCAT
ACAAATGAAT
ACAAACTAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
ACAAACGAAT
610
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTCTTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTTTTTATTT
TTTTTTATTT
TTTTTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTTTTTTATT
TTATTTATTT
TTTTTTATTT
TTATTTATTT
TTATTTATTT
TTATTTATTT
TTTTTAATTT
TTATTTATTT
TTATTTATTT
620
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
CTAGTATTAA
630
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
ATGTTCATGC
640
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
AGTAAGAATA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
650
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
660
670
680
GGCCGAGTAT TCCACCTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
GGCCGAGTAT TCCACTTTTT TTCGAGTCCT
690
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
G?TTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
700
TAAATAGGGT
TAAATAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
710
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
720
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
132
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATG
AGAGGGGATG
AGAGGGGATG
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATG
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
AGAGGGGATT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCTGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
GGCCGAGTAT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TCCACTTTTT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
TTCGAGTCCT
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
GTTTAGGGAA
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TAAACAGGGT
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTAAAATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTAAAATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TT?AAATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTTGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTAGATGTA
TTTTGATGTA
TTTAGATGTA
TTTAGATGTA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
TACATAGAGA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
730
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
740
750
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
CAATGAAAAA TGCAAGCACG
760
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
770
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
AGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
780
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
T-CTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACA
TTCTTTAACG
TTCTTTAAC-
790
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
GCTTTGGTTT
GCTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGATTT
??TTTGGTTT
800
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
133
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
AAGCCGTGTG
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
CAATGAAAAA
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
TGCAAGCACG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GTTTGGGGAG
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
GGATTTTTTA
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTT-AACG
TTCTTTAACG
TTCTTTAACG
TTCTTT---G
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTT----G
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
TTCTTTAACG
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
ACTTTGGTTT
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
TAGTGTATAC
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
810
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
820
830
AAAATAAAGG GGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
AAAATAAAGG AGCAATATAT
840
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
850
T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------T--------TTTAGTTTAT
TTTAGTTTAT
TTTAGTTTAT
T---------
860
---------T
TTAG-----TTAGATTTAG
TTCGATTTAG
TTCGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTCGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
870
TAGTCTTTTT
---TCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
880
TTTT---CGT
TTTT---CGT
TTT----CGT
TTT----CGT
TTT----CGT
TTTT---CGT
TTTT---CGT
TTTT---CGT
TTTT---CGT
TTTT---CGT
TTTTT--CGT
TTTTT--CGT
TTTTT--CGT
TTT----CGT
TTT----CGT
TTT----CGT
TTT----CGT
TTTTT--CGT
TTTTT--CGT
TTTTT--CGT
TTTTT--CGT
TTTTT--GGT
TTTTTT-CGT
TTTTTTTCGT
TTTTTT-CGT
134
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
CGAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
-GAGTTTTTG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AAAATAAAGG
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
AGCAATATAT
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TGCTCCTTTA
TTTAGTTTAT
T--------T--------T--------T--------T--------T--------T--------TTTAGTTTAT
T--------T--------T--------TTTAGTTTAT
TTTAGTTTAT
T--------T--------T--------TTTAGTTTAT
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TTAGATTTAG
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TAGTCTTTTT
TTTTT--CGT
TTTTT--CGT
TTTTT--CGT
TTTT---CGT
TTTTT--CGT
TTTTTT-CGT
TTTTT--CGT
TTTTT--CGT
TTTTTT-CGT
TTTT---CGT
TTTTTT-CGT
TTTT---CGT
TTTTTT-CGT
TTTTT--CGT
TTT----CGT
TTTT---CGT
TTTT---CGT
TTTTTT-CGT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
890
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
900
910
TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TTTCTTTACA TTTATATA-TTTCTTTACA TTTATATA-TTTCTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTAAATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATATA
TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA-TT-CTTTACA TTTATATA--
920
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTGG
----AGTTTG
----AGTTTG
----AGTTTG
TATAAGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
930
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
CTTTTTCTTT
CTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
940
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACCT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACCT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
950
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
960
CCTTATTTAT
CCTTATTTAT
CCTTATTTAT
CCTTATTTAT
CCTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CCTTATTTAT
CCTTATTTAT
CCTTATTTAT
CCTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
135
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TACACTTTTT
TT-CTTTACA
TT-CTTTACA
TT-CTTTCCA
TT-CTTTACA
TT-CTTTACA
TT-CTTTACA
TT-CTTTACA
TT-CTTTACA
TT-CTTTACA
TTTACATA-TTTATATA-TTTATATA-TTTATATA-TTTATATA-TTTATATA-TTTACATA-TTTATATA-TTTATATA--
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
----AGTTTG
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
TTTTTTCTTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
ACTTTAACTT
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
TTTTATTTTA
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
CTTTATTTAT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
970
TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-TACCTTA
TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT-------TT--------
980
-------CTT
-------CTT
-------CTT
-------CTT
-------CTT
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTT
-------CTT
-------CTT
-------CTT
-------CTT
-------CTT
TTTATTTCTT
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
-------CTG
990
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
GATTATCATA
ATTATCATAA
ATTTTGATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
ATTATCATAA
1000
GAAATCAAAA
GAAATCAAAA
GAAAT-AAAA
GAAAT-AAAA
GAAAT-AAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAC
GAAATCAAAC
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
A?AAATCAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
GAAATCAAAA
1010
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAAAAA--GG
AAAAAAA-GG
AAAAAAAAGG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA?----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
AAA-----GG
1020
ATTTT-AAAT
ATTTT-AAAT
ATTTTT-AAT
ATTTTT-AAT
ATTTTT-AAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTT-AAT
ATTTTT-AAT
ATTTTT-AAT
ATTTTT-AAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
?TTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
ATTTTTTAAT
1030
GAGTTTTATT
GAGTTTTATT
GAGTTTGATT
GAGTTTGATT
GAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
GAGTTTGATT
GGGTTTGATT
GGGTTTGATT
GAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTTATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTTATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
AAGTTTGATT
1040
TCGTTTTATT
TCGTTTTATT
TCGTTTTATT
TCGTTTTATT
TCGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TCGTTTTATT
TCGTTTTATT
TCGTTTTATT
TCGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
TGGTTTTATT
136
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
1050
TGGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
-GGATTTGAC
1060
TTAATATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAATATTAT
TTAATATTAT
TTAATATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAA
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
TTAGTATTGT
TTAGTATTAT
TTAGTATTAT
TTAGTATTAT
1070
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGT-ACGTTCGT-ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGCTA
ACGTTCGCTA
ACGTTCGCTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGT?CGTTA
ACGTCCGTTA
ACGGTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
ACGTTCGTTA
1080
TGTAAGAATT
TGTAAGAATT
TGTAATAATA
------------------TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
?GTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATG
TGTAATAATA
TGTAATAATA
TGTAATAATA
TGTAATAATA
1090
CGTTATGTAA
CGTTATGTAA
TA--A----------------------TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA---TA
TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA---TA
TATTA----TATTA----TATTA----TATTA----TATTA----TATTA----TATTA-----
1100
TAATAT---TAATATACGT
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGTTATGTAA
------------------------------------------------------------------------------------------CGTTATGTAA
----------------------------------------------------------------
1110
-AATATATTA
TATTATATAT
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAATATATTA
------------------------------------------------------------------------------------------TAATATATTA
----------------------------------------------------------------
1120
TATATTAACTAACGTAACTATATTAAC------------------TATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACT
TATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAAC?A?ATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAACTATATTAAC-
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
1130
----------------------------------------------------------------
1140
----------------------------------------------------------------
1150
----------------------------------------------------------------
1160
--AAATTTTT
--GAATTTTT
--GAATTTTT
--------------------GAATTTTT
--GAATTTTT
1170
TT------AA
TT------AA
T------AAA
------------------TT------AA
TT------AA
1180
AA--TATAAA
AA--TATAAA
AA--TAAAAA
------------------AA--TATAAA
AA--TATAAA
1190
AAAAA----AAAAA----AAAAA----------------------AAAAA----AAAA------
1200
--------GT
--------GT
--------GT
-----AAAGT
-----AAAGT
--------GT
--------GT
137
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
------------------------------------------------------ATTAACGAAT
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
------------------------------------------------------TTTTTTAAAA
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
------------------------------------------------------TATAAAAATA
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
ACGAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
--GAATTTTT
TT------AA
TT------AA
TT------AA
T-------AA
--------AA
--------AA
TT------AA
TTTTT---AA
TTTTT---ATTTT----AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TTAATTTTAA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
T-------AA
TTTTT---AA
TT------AA
TT------AA
TT------AA
TT------AA
TT------AA
TTT-----AA
TTT-----AA
TT------AA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AAA-TATAAA
AAAATATAAA
AAAATATAAA
AA--TATAAA
----TATAAA
----TATAAA
----TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AAA-TATAAA
----TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
AA--TATAAA
A---TATAAA
A---TATAAA
AA--TATAAA
AAAA-----AAAAA----AAAA-----AAAA-----AAAA-----AAAA-----AAAAAAA--AAAAA----AAAAA----AAAAA----AAA------AAAAA----AAAAA----AAAAA----AAAAA----AAAA-----AAAAA----AAAA-----AAAAAAA--AAAAA----AAAAA----AAAA-----AAAAA----AAAA-----AAAAA----AAAAA----AAAA-----AATAAAA--AAAA-----AAAA-----AAAAA----AAAAA----AAAA-----AATAAAAAAA
AAAA-----AATAAA----
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
AAAGTAAAAT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
--------GT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
1210
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTG
AAAAAAGTTG
AAAAAAGTTG
AAAAAAGTTG
AAAAAAGTTG
AAA------AAA------AAA------AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
1220
TTACCAATCT
TTACCAATCT
TTACCATTCT
TTACCAATTT
TTACCAATTT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
---------------------------T
TTACCAATCT
TTACCAATCT
TTACCAATCT
1230
TTTGTAACGT
TTTGTAACGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
---------------------TTAAA-TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
1240
TTTTATTTTTTTTATTTTTTTTATTTTTTTTATTTTTTTTATTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTT-----------T
---------T
---------T
TTTTATTTTTTTTATTTTTTTTATTTT-
1250
-AGAGTCAAA
-AGAGTCAAA
-AGAGTAAAA
-AG?GTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
TAGAGTAAAA
TAGAGTAAAA
TAGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
1260
ATAAATAAAA
ATAAATAAAA
ATAAATACAA
ATAAATAAAA
ATAAATAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAATAAAA
ATAAATAAAA
ATAAATAAAA
1270
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AG?AAAGATA
AGAAAAGATA
AGAAACGATA
AGAAAAGATA
AGAAAAGATA
AGAAACGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGAGA
AGAAAAGAGA
1280
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
138
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AAAAAAGTTC
AAAAAAGTTG
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAA------AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTG
AAAAAAGTTC
AAA------AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAAAAAGTTC
AAA------AAA------AAAAAAGTTC
AAAAAAGTTC
AAAAAGGTTG
AAAAAAGTTC
AAAAAAGTTG
AAAAAAGTTC
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAAT?T
TTACCAATCT
TTACCAATCT
---------TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATTT
TTACCAATCT
---------TTACCAATCT
TTACCAATCT
TTACCAATCTTACCAATCT
------------------TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTACCAATCT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
---------TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
---------TTTGTAAAGT
TTTGTAAAGT
---------TTTGTAAAGT
------------------TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTGTAAAGT
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTT
TTTTTTTTTTTTTTTTTT---------T
TTTTTTTTTT
TTTTTTTTTTTTTTTTTTT
TTTTTTTT-TTTTTTTT----------T
TTTTTTTT-TTTTTTTTT---------TTTTTTTTT---------T
---------T
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTTTTTTTTT
TTTTTTTT--
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
TAGAGTAAAA
TAGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AG?GTAAAA
-AGAGTAAAA
TAGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
----------AG?GTAAAA
TAGAGTAAAA
TAGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
-AGAGTAAAA
ATAAATAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
---------AGAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
ATAAAGAAAA
AGAAAAGAGA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
---------------GATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
---------AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
AGAAAAGATA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
----TTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
---------GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
GAGATTGGAA
TAGATTGGAA
GAGATTGGAA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
1290
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTATATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
1300
ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTAATAT
ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA----
1310
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
CTTATCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
1320
AAATAAGAGA
AAATAAGAGA
AAATAAGAGA
AAATAAGAGA
AAATAAGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATAAGAGA
AAATAAGAGA
AAATAAGAGA
AAATAAGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
1330
AAAAACTAAT
AAAAACTAAT
AAAAACTAAT
AAAAACTAAT
AAAAACTAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACTAAT
AAAAACTAAT
AAAAACTAAT
AAAAACTAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
1340
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
1350
AGAAATTAAT
AGAAATTAAT
AGAAATTGAT
AGAAATTGAT
AGAAATTGAT
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTTAT
AGAAATTGAT
AGAAATTGAT
AGAAATTGAT
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
1360
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
139
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
---TCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
TTTTCTATAT
ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA---ATCTTA----
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
----TCTCAG
AAATACGAGA
CAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATAC?AGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAATACGAGA
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAACGAAT
AAAAAC---AAAAACGAAT
AAAAACGAAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGAT
GATGAATGGT
---GAATGAT
GATGAATGAT
GATGAATGAT
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGA?
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
AGAAATTGAG
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
ATATTTGAAA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
1370
TATTTAAA-C
TATTTAAA-C
TATTTAAA-C
TATTTAAA-C
TATTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAC-C
TATTTAAA-C
TATTTAAA-C
TATTTAAA-C
TATTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAAAC
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
1380
AATCAAAAAA
AATCAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATCAAAAGA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
1390
AA-TACTATA
AA-TACTATA
---TAC------TAGTATA
---TAGTATA
A------ATA
A--TACTATA
A--TACTATA
AA-TACTATA--TACTATA
AA-TACTATA
AA-TACTATA
AA-TACTATA
A--TACTATA
A--TACTATA
A--TACTATA
A--TACTATA
A--TACTATA
AA-TACTATA
AAATACTATA
AAATACTATA
AAATACTATA
AA-TACTATA
A--TACTATA
A--TAC?AT?
A--TACTATA
AA-TACTATA
AA-TACTATA
A--TACTATT
AAATACTATA
AAATACTATA
AAATACTATA
AA-TACTATA
AAATACTATA
1400
CTATTCAAAA
CTAT--------------CTATA----CTATA----CTAT-----CTAT-----CTAT--------------CTAT-----CTATTATAAA
CTATT----CTAT-----GTAT-----GTAT-----GTAT-----GTATATAATA
CTAT-----CTATT----CTATT----CTATT----CTATT----CTATT----CTATT----CTATT----CTATT----CTATT----CTAT-----ATAGTAG--CTATT----CTATT----CTATT----CTAT-----CTATT-----
1410
AAAA--------------------------------------------------------------------------------------AGAGGGAAAA
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
1420
----TACTAT
---------------------------------------------------------------------------------AAAATACTAT
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
1430
ACTA-TATAG
------ATAG
-----TATAG
--GTATATAG
--GTATATAG
-----TATAG
-----TATAG
-----TATAG
-----TATAG
-----TATAG
ACTATTATAA
------ATAA
-----TATAA
------ATAG
------ATAG
------ATAG
--GTATATAG
-----TATAG
------ATAG
------ATAG
------ATAG
------ATAG
------ATAG
------ATAG
------ATAA
------ATAG
------ATAG
-----TATAG
------ATAG
------ATAG
------ATAA
------ATAG
-----TATAG
------ATAG
1440
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
AAGAGGG--AAGAGGGTCA
AAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGG--TAGAGGGTCA
TAGAGGG--TAGAGGGTCA
TAGAGGGTCA
TA?AGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
140
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
TTTTTAAA-C
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
AATAAAAAAA
A--TACTATA
A--TACTATA
AA-TACTATA
AA-TACTATA
AAATACTATA
A--TACTATA
A--TACTATA
A--TACTATAA-TACTATA
CTATT----CTATT----CTATA----CTATT----CTAT-----CTATT----CTATT-------------CTATT-----
----------------------------------------------------------------------------------
----------------------------------------------------------------------------------
------ATAG
------ATAA
-----TATAA
------ATAG
-----TATAG
------ATAG
------ATAG
-----TATAG
------ATAG
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
TAGAGGGTCA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
1450
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
---------AATAATAGAA
AATAATCGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGGA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
---------AATAATAGAA
---------AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
AATAATAGAA
1460
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGT?TC
---------ATTTTGT?TC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
---------ATTTTGTATC
---------ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
ATTTTGTATC
1470
CATATAAATT
CATATAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
---------CATAGAAATT
CATAGAAATT
CATAGAGATT
CA-AGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
---------CATAGAAATT
---------CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
CATAGAAATT
1480
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
---------CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CATCAAACTA
CATCAAACTA
CATCAAACTA
CATCAAACTA
---------CATCAAACTA
---------CATCAAACTA
CATCAAACTA
CATCAAACTA
CAGCAAACTA
CATCAAACTA
CAGCAGACTA
CATCAAACTA
CATCAAACTA
CATCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CATCAAACTA
CATCAAACTA
CAGCAAACTA
CAGCAAACTA
CAGCAAACTA
CATCAAACTA
1490
TTACGATCTT
TTACGATCTT
TTACAATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
---------TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
---------TTACGATCTT
---------TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
TTACGATCTT
1500
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
---------ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
---------ATCTATTAAC
---------ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
ATCTATTAAC
1510
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTGG
TATCTATTAG
TATCTATTAG
---------TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
---------TATCTATTAG
---------TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
AATTTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
TATCTATTAG
AATCTATTAG
TATCTATTAG
TATCTATTAG
1520
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
---------TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
---------TTATTCATAA
---------TTATTCATAA
TTATTCATAA
TTATCCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
TTATTCATAA
141
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
1530
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
---------CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
---------CTACTATGAA
---------CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGGA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
CTACTATGAA
1540
TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT--------------TTAT-----TTAT-----TTAT-----TTATTAATAT
TTATTAATAT
TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT--------------TTAT--------------TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT-----TTAT------
1550
------------------------------------------------------------------------------------------------------------------------------GAATTATAGA
GAATTATAGA
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
1560
--TAATATGA
--TAATATGA
-----------TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
-----------TAATATGA
--TAATATGA
--TAATATGA
ATTAATATGA
ATTAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
-----------------------------TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
--TAATATGA
1570
ATTATAGACT
ATTATAGACT
-----AGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
---------ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
---------------------------ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
ATTATAGACT
1580
AGAATTTAGA
AGAATTTAGA
AGAATTTAGA
AGAATTTAGA
AGAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
---------ACAATTTAGA
ACAATTTAGA
AGAATTTAGA
AGAATTTAGA
AGAATTTAGA
AGAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
---------------------------ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
ACAATTTAGA
1590
AAAA-CTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
---------AAAAACTAGA
AAAAACTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAA-CTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
---------------------------AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTAGA
AAAAACTATA
AAAAACTAGA
AAAAACTATA
AAAAACTAGA
1600
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAAGT
ATTTCTAAGT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
---------ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCGAATT
ATTTCGAATT
ATTTCGAATT
ATTTCGAATT
-----------T---A-----------ATTTCGAATT
ATTTCGAATT
ATTTCGAATT
ATTTCTAATT
ATTTCGAATT
ATTTCTAATT
ATTTCGAATT
ATTTCGAATT
ATTTCGAATT
ATTTCTAATT
ATTTCTAATT
ATTTCGAATT
ATTTCGAATT
ATTTCGAATT
ATTTCTAATT
ATTTCTAATT
ATTTCTAATT
ATTTCGAATT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
1610
TTTAATGCCA
TTTAATGCCA
TTTAATGTCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
1620
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
1630
TAACATATTT
TAACATATTT
TAACATATTT
TAACATATTT
TAACATATTT
GAACATATTT
GAACATATTT
1640
AATATAATAAATATAATAAATATAATAAATATAATAAATATAATAAATATAATAA
AATATAATA-
1650
---------------------------------------------TAATA--------------
1660
----------------------------------------------------------------
1670
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
1680
ATGGTAGATT
ATGGTAGATT
ATAGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
142
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
---------TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
---------------------------TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTGAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TTTAATGCCA
TACTT-AATA
TACTT-AATA
TACTT-AATA
---------TGCTT-AATA
TGCTT-AATA
TACCTTAATA
TACTT-AATA
TACTTTAATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
---------------AATA
---------TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TGCTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TACTT-AATA
TGCTT-AATA
TGCTT-AATA
TACTT-AATA
TACTTTAATA
TACTT-AATA
TACTT-AATA
TACCTTAATA
TACTT-AATA
GAACATATTT
GAACATATTT
GAACATATTT
---------GAACATATTT
GAACATATTT
TAACATATTT
TAACATATTT
TAACATATTT
TAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
---------GAACATATTT
---------GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
GAACATATTT
AATATAATAAATATAATAA
AATATAATAA
---------AATATAATAA
AATATAATAA
AATATAATAAATATAATAT
AATATAATAT
AATATAATAAATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
---------AATATAATAA
---------AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
AATATAATAA
---------TAATA----TAATA-------------TAATA----TAATA-------------ATAATAATGATAATAATG---------TAATA----TA-------TA-------TA-------TA----------------TA----------------TA-------TA-------TA-------TAATA----TA-------TAATA----TA-------TA-------TA-------TAATA----TAATA----TAATATATAA
TA-------TA-------TAATA----TAATA----TAATA----TA--------
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAATATAATA
-------------------------------------------------------
---TATAATA
---TATAATA
---TATAATA
------------TATAATA
---TATAATA
---TATAATA
--ATATAATA
--ATATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
------------TATAATA
------------TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TATAATA
---TCTAATA
---TATAATA
ATATATAATA
---TATAATA
---TATAATA
---TATAATA
---TCTAATA
---TATAATA
---TATAATA
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
---------ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
---------ATGGTAGATT
---------ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
ATGGTAGATT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
1690
CAATCTAATG
CAATCTAATA
CAATCTAATA
CAATCTAATA
CAATCTAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
---------CAATCGAATA
CAATCGAATA
CAATCTAATA
CAATCTAATA
CAATCTAATA
1700
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
---------TTCAATTTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
1710
---------------------------TATTCAATCT
TATTCAATCT
----------------------------------------------------------------------------------------------------
1720
--TGTTGATA
--TGTTGATA
--TGTTGATA
AATGTTGATA
AATGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
-----------TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
1730
GAATATAATA
GAATATAATA
GAATAGAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
---------GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAAT-
1740
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
---------ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
----------
1750
TAATA----TAATA----GAATA----GAATA----GAATA----GAATATAATGAATATAATGAATATAATGAATATAATGAATATAAT---------GAATATAATT
GAATATAATT
GAATATAATGAATATAAT----------
1760
-------TAA
-------TAA
-------TAA
-------TAA
-------TAA
------------------------------------------------------AAGAATATAA
AAGAATATAA
----------------------------
143
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
CAATCTAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
---------CAATCGAATA
---------CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
CAATCGAATA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
---------TTCAATCTAA
---------TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
TTCAATCTAA
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
-----------TGTTGATA
-----------TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
--TGTTGATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
---------GAATATAATA
---------GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
GAATATAATA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
---------ATATAATTAA
---------ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
---------ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
ATATAATTAA
GAATATAATGAATATAATGAATATAATT
GAATATAATT
GAATATAATT
GAATATAATT
---------GAATATAATT
---------GAATATAATT
GAATATAATT
TAATATAATT
GAATATAATGAATATAATT
GAATATAATT
GAATATAATT
GAATATAATT
GAATATAATT
GAATATAATT
--TTCTAATT
GAATAGAATT
GAATATAATT
GAATATAATT
GAATATAATTAATATAATT
GAATATAATCAATATAATT
------------------AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
---------AAGAATATAA
---------AAGAATATAA
AAGAATATAA
AAGAATATAA
---------AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
AAGAATATAA
---------AAGAATATAA
---------AAGAATATAA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
1770
TAATTCTATT
TAATTCTATT
TAATTCTATT
TAATTCTATT
TAATTCTATT
-AATTCTCTT
-AATTCTCTT
-AATTCTCTT
-AATTCTCTT
-AATTCTCTT
---------TAATTATCTT
TAATTCTCTT
-AATTCTATT
-AATTCTTAT
-AATTCTTAT
-AATTCTATT
-AATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
---------TAATTCTCTT
----------
1780
TAAAATTTAA
TAAAATTTAA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
---------TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TCAAATTTCA
TAAAATTTCA
---------TAAAATTTCA
----------
1790
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
---------AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAAATTATT
AAAAATTATT
AAAAATTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
---------AAAA-TTATT
----------
1800
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
---------TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTTTTT
TTAATTTTTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
---------TTAATTATTT
----------
1810
A--------A--------ACTTCTATTT
ACTTCTATTT
ACTTCTATTT
AATTCTATTT
AATTATATTT
AATTATATTT
AATTCTATTT
AATTATATTT
---------AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTATATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
---------AATTCTATTT
----------
1820
------------------GATTCTATTT
G--------G--------------------------------------------------------------G--------G--------G--------------------------------------------G--------G--------G--------G-----------------G------------------
1830
--------AT
--------AT
TATATTTGAT
--------AT
--------AT
--------------------------------------------------------------AT
--------AT
--------AT
--------------------------------------------AT
--------AT
--------AT
--------AT
-----------------AT
----------
1840
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TATATTTT?A
TATATTTT?A
-------T?A
-------T?A
-------T?A
-------T?A
-------T?A
--------?TCTATTTT?A
TCTATTTT?A
TATATTTT?A
-------T?A
-------T?A
-------T?A
-------T?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
--------?TCTATTTT?A
--------?-
144
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
-AATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
TAATTCTCTT
-AATTCTCTT
TAATTCTCTT
-AATTCTCTT
TAATTCTCTT
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TCAAATTTCA
TAAAATTTCA
TCAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
TAAAATTTCA
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
AAAA-TTATT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
TTAATTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTATATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
AATTCTATTT
G--------G--------G-----------------G--------G--------G--------G--------G--------G--------G--------G--------G--------G-----------------G-----------------G---------
--------AT
--------AT
--------AT
-----------------AT
--------AT
--------AT
--------AT
--------AT
--------AT
--------AT
--------AT
--------AT
--------AT
-----------------AT
-----------------AT
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
-------T?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
TCTATTTT?A
-------T?A
TCTATTTT?A
-------T?A
TCTATTTT?A
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
1850
ATCATATTAA
ATCATATTAA
ATCATATTAA
ATCATATTAA
ATCATATTAA
AGCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
---------ATCATAGTCA
ATCATAGTCA
ATCATATTAA
ATCATATTAA
ATCATATTAA
ATCATATTAA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
---------ATCATAGTCA
---------ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
1860
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
---------AAATTATATT
AAATTATATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
---------AAATTAGATT
---------AAATTAGATT
AAATTAGATT
AAATTGGATT
AAATTAGATT
AAATTAGATT
AAATTATATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
1870
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
---------TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
---------TTAATTTAAT
---------TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
1880
CATAATATAT
CATAACATAA
CATAACA-AA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
---------CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
---------CATAACATAA
---------CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
1890
TAT------T
TATA-TAATT
TATA-TCATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
---------TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATAATTATT
TATA-TTATT
---------TATA-TTATT
---------TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
1900
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
---------AATATTATAA
AATATTATAA
AATATTCTAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
---------AATATTATAA
---------AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
1910
TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--------?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--------?--TTATTA?--------?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATAA?TTA
TTATTA?---
1920
---------T
---------T
---------T
---------T
---------T
---TGTTCCA
---TGTTCCA
---TGTTCCA
---TGTTCCA
---TGTTCCA
------------TGTTCTA
---TGTTCTA
---------T
---------T
---------T
---TGTTATT
---TGTTCCA
---TGTTCTA
---TGTTCTA
---TGTTCTA
---TGTTCTA
------------TGTTCTA
------------TGTTCTA
---TGTTCTA
---TGTTCTA
---TGTTCCA
---TGTTCTA
---TGTTCTA
---TGTTCTA
TTATGTTCTA
---TGTTCTA
145
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
ATCATAGTCA
AAATTAGATT
AAATTAGATT
AAATTATATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
AAATTAGATT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
TTAATTTAAT
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
CATAACATAA
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
TATA-TTATT
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
AATATTATAA
TTATTA?--TTATTA?--TTATTA?--TTATTA?--TTATAA?TTA
TTATTA?--TTATTA?--TTATTA?--TTATTA?---
---TGTTCTA
---TGTTCTA
---TGTTCTA
---TGTTCTA
TTATGTTCTA
---TGTTCCA
---TGTTCTA
---TGTTCCA
---TGTTCTA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
1930
GTTATATTTA
GTTATATTTA
GTTATATTTA
GTTATATTTA
GTTATATTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
---------------TTTA
------TTTA
GTTATATTTA
GTTATATTTA
GTTATATTTA
GTTATATTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
---------------TTTA
---------------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
------TTTA
1940
TTCTATTAGA
TTCTATTAGA
TTCTATTAGT
TTCTATCAGG
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGA
TTCTATTAGT
TTCTATTAGT
---------TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
---------TTCTATTAGT
---------TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
TTCTATTAGT
1950
ATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATT
ATATTCTATATATTCTAT---------ATATTCTATATATTCTATT
ATATTCCATATATTCTATT
ATATTCTATT
ATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTAT---------ATATTCTATA
---------ATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATT
ATATTCTATATATTCTATT
ATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTATATATTCTAT-
1960
---------------------------------------------------------------TATAA----------------------------------------TAGAATTAGA
---------TAGAATTAGA
TAGAATTAGA
---------------------------------------------------------------------------------------------------------------------TATTCTATTA
---------TATTCTATTA
----------------------------------------------------------------------------------------------------
1970
------------------------------------------------------------------------------------------------------------ATATTCTATA
---------ATATTCTATA
ATATTCTATA
---------------------------------------------------------------------------------------------------------------------GTATATTCTA
---------GTATATTCTA
----------------------------------------------------------------------------------------------------
1980
------------------------------------------------------------------------------------------------------------TATATTACTA
---------TATATTAGTA
TATATTAGTA
---------------------------------------------------------------------------------------------------------------------T-----------------T------------------------------------------------------------------------------------------------------------
1990
------------------------------------------------------------------------------------------------------------TAATATAGTA
---------TAATCATAGT
TAATCATAGT
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2000
------------------------------------------------------------------------------------------------------------TTCTTATAGT
---------AATATTCTAG
AATATTCTAG
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
146
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
2010
------------------------------------------------------------------------------------------------------------TATAATAAAA
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2020
-------------------------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
----------------TTA
TTACTAA---------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
----------------TTA
----------------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
-------TTA
2030
------------------GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAA------T
GAATTAGAAT
GAATTAGAAT
---------GAATTAGAAT
---------GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
---------GAATTAGAAT
---------GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAGT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTAGAAT
GAATTATAAT
GAATTAGAAT
GAATTATAAT
GAATTAGAAT
2040
-------ATA
-------ATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
---------ATTCTATATA
-TTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
---------ATTCTATATA
---------ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
ATTCTATATA
2050
TAT--TAGTC
TAT--TAGTA
TAT--TAGTA
TAT--TAGTA
TAT--TAGTA
TATAATACTA
TATAATACTA
TATAATACTA
TAA--TACTA
TATAATACTA
---------TAT--TACTA
TAT--TACTA
TAT--TAGTA
TAT--TAGTA
TAT--TAGTA
TAT--TAGTA
TATAATACTA
TAA--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
---------TAT--TACTA
---------TAT--TACTA
TAT--TACTA
TAT--TACTA
TATAATACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TAT--TACTA
TATAATACTA
TAT--TACTA
TATAATACTA
TAT--TACTA
2060
TAAT-ATAGT
TAAT-ATAGT
TATT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
---------TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAATCATAGT
TAATCATAGT
TAATCATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
---------TAAT-ATAGT
---------TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-AAAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
TAAT-ATAGT
2070
AATATTATAT
AATAATATAT
AATACTATAA
AATATTATAA
AATATTATAA
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
---------ATTCTTATAATTCTTATAAATATTA--AATATTCTAA
AATATTCTAA
AATATTCTAA
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
---------ATTATTATAT
---------ATTATTATAT
ATTATTATAT
ATTATTTTAT
ATTATTATAT
ATTATTATAT
ATTATATTAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
ATTATTATAT
2080
TATTA----TATATTATTA
-TATAATATA
-TATATTATA
-TATAATATA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
----------GTTATAAGA
-GTTATAAGA
-----TACTA
TATATTACTA
TATATTACTA
TATATTACTA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
T-TAT-AATA
---------T-TAT-AATA
---------T-TAT-AATA
TTATAATATA
T---T-AATA
T-TAT-AATA
T-TAT-AATA
AGTTATAATA
T-TAT-AATA
T-TAT-AATA
TTATAATATA
TG--ATAATA
T-TATAAATA
-GTTATAATA
T-TAT-AATA
T-TAT-AATA
T-TCTAATAT
T-TATAATAA
T-TCT-AATA
T-TAT-AATA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
2090
------------------TTATAAA--T--------T--------TA-------TA--------
2100
----------------------------------------------------------------
2110
------TACT
------TACT
---------------TACT
------TACT
----ATTACT
----ATTACT
2120
AATAGTACTC
AATAGTACTC
--TAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
2130
GACCTTTTTT
GACCTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
2140
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
TTTCTATTTT
TT-CTATTTT
TTTCTATTTT
TT-CTATTTT
2150
ATTTT----ATTTT----ATTTT----ATTTT----ATTTT----ATTTTTAATT
ATTTTTAATT
2160
-----GAATT
-----GAATT
-----GAATT
-----GAATT
-----GAATT
TTATTTAATT
TTATTTAATT
147
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TA-------TA-------TA----------------AA-------AA-------TA-------TATTAT---TATTAT---TAATAT---TA-------TA-------TA-------TA-------TA----------------TA----------------TA-------AA-------TAGTATTATT
TA-------TA-------TA-------TA-------TA-------AA-------TA-------TAGTATTATT
TA-------TA-------TA-------AAT------TA-------TA-------TA--------
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTTTTATAA
---------------------------------------------------------------ATATTTATAA
----------------------------------------------------------------
----ATTACT
----ATTACT
----ATTACT
-------------ATTACT
----ATTACT
-----TTACT
----ATTAGT
----ATTAGT
----ATTACT
----ATTACT
----AATACT
----AATACT
----AATACT
----AATACT
-------------AATACT
-------------AATACT
------TACT
AAAAAATACT
----ATTACT
----AATACT
----ATTACT
----AATACT
----AATACT
------TACT
----ATTACT
TATAATTACT
----ATTACT
----AATACT
----AATACT
------TACT
----ATTACT
----ATTACT
----AATACT
AATAGTACTC
AATAGTACTC
AATAGTACTC
---------AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
---------AATAGTACTC
---------AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
AATAGTACTC
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
---------TACTTTTTTT
TACTTTTTTT
GACTTTTTTT
GACTGTTTTT
GACTGTTTTT
GACTATTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
---------GACTTTTTTT
---------GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
AACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
GACTTTTTTT
TT-CTATTTT
TTTCTATTTT
TT-CTATTTT
---------T--CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
T--CTATTTT
T--CTATTTT
T--CTATTTT
TT-CTATTTT
---------T--CTATTTT
---------T--CTATTTT
T--CTATTTT
---CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
---CTATTTT
T--CTATTTT
T--CTATTTT
TT-CTATTTT
TT-CTATTTT
TT-CTATTTT
T--CTATTTT
T--CTATTTT
TTTCTATTTT
TT-CTATTTT
TTTCTATTTT
T--CTATTTT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
---------CTTTTTCATT
CTTTTTCATT
ATTTT----ATTTT----ATTTTT---ATTTT----ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
---------ATTTTTAATT
---------ATTTTAAATT
ATTTTTAATT
ATTTTTAATT
ATTTTT---ATTTTTAATT
CTTTTTCATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
CTTTTTCATT
CTTTTT---ATTATTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
ATTTTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
---------TTATTTAATT
TTATTTAATT
-----GAATT
-----GAATT
-----GAATT
-----GAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
---------TTATTTAATT
---------TTATTTAATT
TTATTTAATT
TTATTTAATT
------AATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
--ATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
TTATTTAATT
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
2170
TGA-GTATAT
TGA-GTATAT
TTAAGTATAT
TTA-GTATAT
TTA-GTATAT
TTA------TTA-GTATAT
TTA-GTATAT
TTA------TTA-GTATAT
---------TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TGA-GTCTAT
TGA-GTCTAT
2180
TTTCATTTTA
TTTCATTTTA
TTT------TTTCATTTTT
TTTCATTTTT
---------TTTCATTTTA
TTTCATTTTA
---------TTTCATTTTA
---------TAAAATATTA
TAAAATTTTA
TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
2190
AGTATATTTT
AGTATATTTT
---------AGTTTATTTT
AGTCTATTTT
-GTATATTTT
AGTATATTTT
AGTATATTTT
-GTATATTTT
AGTATATTTT
---------AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
2200
ACATTTAAAT
ACATTTAAAT
ACATTTTAAT
ACATTTAAAT
ACATTTAAAT
-CATTTTAAG
ACATTTAAAT
ACATTTAAAT
-CATTTTAAG
ACATTTAAAT
---------ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
2210
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATTTTTC
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
---------TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
2220
ATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTCTAATTA
ATTTTTTATATTTTTTATATTTTTTATATTTTTTAT---------ATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTTTT
2230
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
TCTAGACTTA
-----AGATT
-----AGATT
-----AGATT
-----AGAAT
--------------AGATT
-----AGATT
-----AGATT
-----AGATT
TTTATAGATT
2240
TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA---------------TAA------TAA------TAA------TAA------TAA-------
148
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TGA-GTATAT
TTA-GTATAT
TTA------TTA------TTA-GTATAT
TTA-GTATAT
---------TTA-GTATAT
---------TTA-GTATAT
TTA------TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAA
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA-GTATAT
TTA------TTA-GTATAT
TTA-GTATAT
TTTCATTTTA
TTTCATTTTA
------------------TTTCATTTTA
TTTCATTTTA
---------TTTCATTTTA
---------TTTCATTTTA
---------TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
TAAAATTTTA
TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
TAAAATTTTA
TTTCATTTTA
TTTCATTTTA
TTTCATTTTA
---------TTTCATTTTA
TTTCATTTTA
AGTATATTTT
AGTATATTTT
-GTATATTTT
-GTATATTTT
AGTATATTTT
AGTATATTTT
---------AGTATATTTT
---------AGTATATTTT
-GTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
AGTATATTTT
-GTATATTTT
AGTATATTTT
AGTATATTTT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
---------ACATTTAAAT
---------ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
ACATTTAAAT
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
---------TATATAGATA
---------TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
TATATAGATA
ATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTAT---------ATTTTTTAT---------ATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTAATTTT
ATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTATATTTTTTAT-
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
--------------AGATT
--------------AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
T-TATAGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGATT
-----AGAGT
-----AGATT
-----AGATT
TAA------TAA------TAA------TAA------TAA------TAA---------------TAA---------------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAA------TAATTTAATC
TAA------TAA------TAA------TAA------TAA------TAA-------
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
2250
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2260
----------------------------------------------------------------------------------------------------------TGT
-------TGT
-------------------------------------------------------------------------------------------------------------
2270
-TTTAATCGA
-TTTAATCGA
-TAAAATTGA
-TTAAATCGA
-TTAAATCGA
TGTTAATGTA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
---------ATTTAATCGA
ATTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
----------TTTAATCGA
----------
2280
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
T--------TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
---------TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
---------TCGATTTAAT
----------
2290
AGATTAAA-AGATTAAA-AGATTAAATT
AGATTAAA-AGATTAAA----------ACATTAAC-ACATTAAC-ACATTAAC-ACATTAAC----------ACATTAAA-ACATTAAA-AGATTAAA-AGATTAAA-AGATTAAA-AGATTAAA-ACATTAAC-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA----------ACATTAAA-----------
2300
------------------ATTTAATAGA
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2310
-----TATAG
-----TATAG
TTAAATATAG
-----TATAG
-----TATAG
--------------TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
--------------TAGAG
-----TAGAG
-----TATAG
-----TATAG
-----TATAG
-----TATAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
--------------TAGAG
----------
2320
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTAAATTTA
ATTAAATTTA
---------ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
---------ATTCAATTTA
ATTCAATTTA
ATTAAATTTA
AGTAAATTTA
AGTAAATTTA
AGTAAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
---------ATTCAATTTA
----------
149
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
---------------------------------------------------------------------------------------------------GATCGATTTA
-------------------------------------------------------
----------------------------------------------------TGT
---------------------------------------------ATACATTAAA
-------------------------------------------------------
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
ATTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
ATTTAATCGA
-TTTAATCGA
-TTTAATCGA
--TTAATCGA
-TTTAATCGA
-TTTAATCGA
-TTTAATCGA
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
TCGATTTAAT
ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAC-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTAAA-ACATTACT-ACATTAAA-ACATTAAC-ACATTAAA--
-------------------------------------------------------------------------------------------------------------------------------------------------------------------
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
-----TAGAG
------AGAG
-----TAGAG
-----TAGAG
-----TAGAG
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCGATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
A--------ATTCAATTTA
ATTCAATTTA
ATTCAATTTA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
2330
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
---------TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
---------TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
---------TTTCTATTTA
---------TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
2340
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
---------GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
---------GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
---------GTTTAGAGTT
---------GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
2350
CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT------------CTAAAT---CTAAAT---CTAAAT---CTAAAT------------CTAAAT---CTAAATTATT
CTAAAT---CGAAAT---CGAAAT---CGAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT------------CTAAAT------------CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT----
2360
------AGAG
------AGAG
------AGAG
------AGAG
------AGAG
---------------------------------------------------------------TAGTTTAGAG
------AGAG
------AGAG
------AGAG
------AGAG
----------------------------------------------------------------------------------------------------------------------------------------------------------
2370
--ATAATTAG
--ATAATTAG
--ATAATTAG
--ATAATTAG
--ATAATTAG
-----------------AG
--------AG
--------AG
--------AG
-----------------AG
TTCTAAATAG
--ATAATTAG
--ATAATTAG
--ATAATTAG
--ATAATTAG
--------AG
--------AG
--------AG
--------AG
--------AG
-----------------AG
-----------------AG
--------AG
--------AG
--------AG
--------AG
--------AG
--------AG
--------AG
--------AG
2380
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
---------AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
---------AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATA
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
---------AGTGAAAATC
---------AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
2390
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
--------CA
TTTTTATGCA
CTTTTATGCA
TTTTTATGCA
TTTTTATGCA
---------TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
---------TTTTTATGCA
---------TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
2400
TTT------A
TTT------A
TTTACATTTA
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
---------TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
---------TTT------A
---------TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
150
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
---------TTTCTATTTA
TTTCTATTTA
TTTCTATTTA
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
---------GTTTAGAGTT
GTTTAGAGTT
GTTTAGAGTT
CTAAAT---CTAAAT---CTAAAT---CTAAAT---CTAAAT------------CTAAAT---CTAAAT---CTAAAT----
----------------------------------------------------------------------------------
--------AG
--------AG
--------AG
--------AG
--------AG
-----------------AG
--------AG
--------AG
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
---------AGTGAAAATC
AGTGAAAATC
AGTGAAAATC
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
---------TTTTTATGCA
TTTTTATGCA
TTTTTATGCA
TTT------A
TTT------A
TTT------A
TTT------A
TTT------A
---------TTT------A
TTT------A
TTT------A
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
2410
TATAT-CATT
TATAT-CATT
TATATACATT
TATATACATT
TATATACATT
TATAATGTAT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
---------TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
---------TATATACATT
---------TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
TATATACATT
-----?CATT
TATATACATT
TATATACATT
TATATACATT
2420
ATATAAATCA
ATATAAATCA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGATATAAATGA
ATATAAATGG
ATATAAATGA
ATATAAATGA
---------ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATCTAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
---------ATATAAATGA
---------ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAGATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
ATATAAATGA
2430
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
--C------TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
---------TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
---------TACATTAACA
---------TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTAACA
TACATTA-CA
TACATTAACA
TACATTAACA
TACATTAACA
2440
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
---------TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
---------TTATAAGTAT
TTATAAGTCT
TTTTAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
---------TTATAAGTCT
---------TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
TTATAAGTCT
2450
CGATAATTAG
CGATAATTAG
CGATAATTAG
CGATAATTAG
CGATAATTAG
--ATAAAAAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
---------AGATAATTAG
AGATAATTAG
CGATAATTAG
CGATAATTAG
CGATAATTAG
CGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
---------AGATAATTAG
---------AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
AGATAATTAG
2460
AATGAAACCC
AATGAAACCC
AATGAAACTC
AATGAAACTC
AATGAAACTC
ATTTTCACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
---------AATGAAACTC
AATGAAACTC
AATGAAACCC
AATGAAACCC
AATGAAACCC
AATGAAACCC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
---------AATGAAACTC
---------AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
AATGAAACTC
2470
CTTTTAGA-CTTTTAGA-TTTTTAGACT
TTTTTAGA-TTTTTAGA-TATTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA----------TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA----------TTTTTAGA----------TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGATA
TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA-TTTTTAGA--
2480
----CTAAAA
----CTAAAA
TAGACTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
-------------CTAAAA
----CTAAAA
----CTAAAA
----CTCAAA
----CTCAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
-------------CTAAAA
-------------CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
GA--CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
----CTAAAA
151
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
2490
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
---------AATTTGAATT
AATTTGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
---------AATTCGAATT
---------AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTTGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTTGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
AATTCGAATT
2500
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAA
ATCTTCGAAT
ATCTTCGAAA
---------ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
---------ATCTTCGAAT
---------ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAT
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAT
ATCTTCGAAA
ATCTTCGAAT
2510
TCGAAAT--TCGAAAT--TCGAAAT--TCGAAAT--TCGAAAT--TCGAAATCGA
TCGAAAT--TCGAAAT--TCGAAATCGA
TCGAAAT-----------TCGAAATCGA
TCGAAATCGA
TCTAAAT--TCGAAAT--TCGAAAT--TCGAAAT--TCGAAAT--TCGAAATCGA
TCGAAATCGA
TCGAAATCGA
TCGAAATCGA
---------TCGAAATCGA
---------TCGAAATCGA
TCGAAATCGA
TCGAGATCGA
TCGAAAT--TCGAAATCGA
TCGAAATCGA
TCGAAATCGA
TCGAAATCGA
TCGAAATCGA
TCGAAAT--TCGAAATCGA
TCGAAAT--TCGAAATCGA
TCGAAATCGA
TCGAAAT--TCGAAAT--TCGAAAT--TCGAAATCGA
2520
---AGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
AA------TG
---AGAAATG
---AGAAATG
AA------TG
---AGAAATG
---------AATAGAAATG
AATAGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
AATAGAAATG
AATAGAAATG
AATAGAAATG
AA------TG
---------AA------TG
---------AATAGAAATG
AATAGAAATG
AATAGAAATG
---AGAAATG
AATAGAAATG
AATAGAAATG
AATAGAAATG
AATAGAAATG
AA------TG
---AGAAATG
AATAGAAATG
---AGAAATG
AATAGAAATG
AATAGAAATG
---AGAAATG
---AGAAATG
---AGAAATG
AATAGAAATG
2530
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
---------AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAG
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
---------AATGGAATAA
---------AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AA-------AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
AATGGAATAA
2540
TTAGTTGTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
---------TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
---------TTAGTTCTAG
---------TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
---------TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
TTAGTTCTAG
2550
CTATTATA-CTATTATA-CTATTAAA-CTATTATA-CTATTATA-CTATTAAATCTATTAAATCTATTAAATCTATTAAATCTATTAAAT---------CTATTA---CTATTA---CTATTAACTCTATTAAATCTATTAAATCTATTAAATCTATTAAATCTATTA---CTATTA---CTATTA---CTATTA------------CTATTA------------CTATTA---CTATTA---CTATTA---CTATTAAATT
CTATTA---CTATTA---CTATTA---CTATTA---CTATTA---CTATTAAATCTATTAAAT---------CTATTA---CTATTA---CTATTAAATA
CTATTAAATCTATTAAATCTATTA----
2560
----------------------------------------------------------AATTAG
----AATTAG
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AAATAATTAG
------------------------------------------------------------------------------------------ATTAGTTCTA
----------------------------
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
2570
------------------------------------------------------TTCTAGCTAT
2580
------------------------------------------------------TAAATAATTA
2590
------------------------------------------------------GTTCTAGCTA
2600
-----------------------TAATT
------------------------AATT
TTAAATAATT
2610
------------------AGTTCTAGCT
------------------AGTTCTAGCT
AGTTCTAGCT
2620
------CTAT
------CTAT
ATTATACTAT
------CTAT
------CTAT
ATTAAACTAT
ATTATACTAT
2630
AAAATAAATA
AAAATAAATA
AAAATTCATA
AAAATTAATA
AAAATTAATA
AAAATAAATA
AAAATAAATA
2640
ATTAATAATG
ATTAATAATG
A-------TG
A-------TG
A-------TG
ATTAATAATG
ATTAATAATG
152
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTCTAGCTAT
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCTAGCTAT
------------------------------------------------------------------------------------------GCTATTAAAT
----------------------------
TAAATAATTA
-----------------------------------------------------------------------------AATTA
-----AATTA
------------------------------------------------------------------------------------------TAAATAATTA
------------------------------------------------------------------------------------------AATTAGTTCT
--------------AATTA
----------
GTTCTAGCTA
------------------------------------------------------------------------GTTCTAGCTA
GTTCTAGCTA
------------------------------------------------------------------------------------------GTTCTAGCTA
------------------------------------------------------------------------------------------AGCTATTA----------GTTCTAGCTA
----------
TTAAATAATT
------AATT
------AATT
---------------------------------AATT
------AATT
------AATT
TTAAATAATT
TTAAATAATT
------------------------------------------------------------------------------------------TTAAATAATT
---------------------------------------------------AATT
------AATT
------------------------------------------AATT
TTAAATAATT
----------
AGTTCTAGCT
AGTTCTAGCT
AGTTCTAGCT
---------------------------AGTTCTAGCT
AGTTCTAACT
AGTTCTAACT
AGTTCTAGCT
AGTTCTAGCT
------------------------------------------------------------------------------------------AGTTCTAGCT
---------------------------------------------AGTTCTAGCT
AGTTCTAGCT
------------------------------------AGTTCTAGCT
AGTTCTAGCT
----------
ATTATACTAT
ATTATACTAT
ATTATACTAT
-------------TACTAT
----TACTAT
ATTATACTAT
ATTATACTAT
ATTATACTAT
ATTATACTAT
ATTATACTAT
----TACTAT
----TACTAT
----TACTAT
----TACTAT
-------------TACTAT
-------------TACTAT
----TACTAT
----TACTAT
ATTATACTAT
----TACTAT
----TACTAT
----TACTAT
----TACTAT
----TACTAT
ATTATACTAT
ATTATACTAT
-------------TACTAT
----TACTAT
----TACTAT
ATTATACTAT
ATTATACTAT
----TACTAT
AAAATAAATA
AAAATAAATA
AAAATAAATA
---------AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
---------AAAATAAATA
---------AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
---------AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
AAAATAAATA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
2650
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
---------AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
2660
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
---------ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
2670
TTATTTTTTT
TTATTTTTTT
TTCTTTTTTT
TTCTTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
---------TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
2680
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
---------–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
2690
A--------A--------A--------ATTTTCTTTT
ATTTTCTTTT
A--------A--------A--------A--------A-----------------A--------A--------A--------A--------A---------
2700
---------------------------TTTA-GTTAT
TTTAGGTTAT
----------------------------------------------------------------------------------------------------
2710
2720
----TTATGG TTATA--------TTATGG TTATA--------TTATGGT TATCTTATT
CTTATTATGG TTATCTTATT
CTTATTATGG TTATCTTATT
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
---------- -------------TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
----TTATGG TTATATCTTA
153
ATTAATAATG
ATTAATAATG
ATTAATAATG
---------ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
---------ATTAATAATG
---------ATTAATAATG
ATTAATACTG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
---------ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
ATTAATAATG
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
---------AATAAATTCA
---------AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
---------AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
AATAAATTCA
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
---------ATTTGCATTT
---------ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTC
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
---------ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
ATTTGCATTT
TTATTTTTTT
TTATTTTTTT
TGATTTTTTT
TGATTTTTTT
TGATTTTTTT
TTATTTTTTT
---------TTATTTTTTT
---------TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TGATTTTTTT
TTATTTTTTT
TGATTTTTTT
TGATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
---------TTATTTTTTT
TGATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
TTATTTTTTT
–AGTTATCTT
–AGTTATCTT
TAGTTATCTT
TAGTTATCTT
TAGTTATCTT
–AGTTATCTT
---------–AGTTATCTT
---------–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
TAGTTATCTT
–AGTTATCTT
TAGTTATCTT
TAGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
---------–AGTTATCTT
TAGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
–AGTTATCTT
A--------A--------A--------A--------A--------A-----------------A-----------------A--------A--------A--------A--------A--------A--------A--------A--------A--------A--------A-----------------A--------A--------A--------A--------A--------A---------
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
-------------TTATGG
-------------TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
-------------TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
----TTATGG
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
---------TTATATCTTA
---------TTATATCTTA
TTATATCTTA
TTATATCTTA
TTAT-----TTATACCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTAT--------------TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
TTATATCTTA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
2730
------------------ATGGTTATAATGGTTATAATGGTTATATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTA---------TTATGGTTAT
TTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTA---------TTATGGTTA----------
2740
---------------------------------------------------------TATCTTA
---TATCTTA
---------------------------TTATATCTTA
------------------------------------------------TATCTTA
---TATCTTA
---TATCTTA
---TATCTTA
---------------------TATCTTA
----------
2750
------------------------------------------------------T
TTATGGTTAT
TTATGGTTAT
---------T
---------T
---------TTATGGTTAT
---------T
---------T
---------T
---------T
---------T
TTATGGTTAT
TTATGGTTAT
TTATGGTTAT
TTATGGTTAT
---------T
---------TTATGGTTAT
----------
2760
-TAGGATAGT
-TAGGATAGT
-TAGGATAGT
-TAGGATAGT
-TAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
---------ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
---------ATAGGATAGT
----------
2770
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
---------CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
---------CTAATAAAAG
----------
2780
GATAAGAATC
GATAAGAATC
GATAAGAATC
GATAAGAATC
GATAAGAATC
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
---------GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATC
GATAAGAATC
GATAAGAATC
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
---------GATAAGAATA
----------
2790
AAAATGAAAT
AAAATGAAAT
AAAATAAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
---------AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATAAAAT
---------AAAATGAAAT
----------
2800
TAAAGAAAAA
TAAAGAAAAA
TCAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
---------TAAATAAAAA
TAAATAAA?A
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
---------TAAAGAAAAA
----------
154
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TTATGGTTATTATGGTTATTATGGTTA---------TTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTA------------------TTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTATTATGGTTA-
---TATCTTA
---TATCTTA
---------------------TATCTTA
------------TATCTTA
---TATCTTA
---TATCTTA
-------------ATCTTA
------------TATCTTA
---TATCTTA
---TATCTTA
------------TATCTTA
---TATCTTA
TTATGGTTAT
TTATGGTTAT
---------T
---------TTATGGTTAT
---------T
TTATGGTTAT
TTATGGTTAT
TTATGGTTAT
---------T
TTATGGTTAT
---------TTATGGTTAT
TTATGGTTAT
TTATGGTTAT
---------T
TTATGGTTAT
TTATGGTTAT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
---------ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
ATAGGATAGT
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAGAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
---------CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
CTAATAAAAG
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
---------GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
GATAAGAATA
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
---------AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
AAAATGAAAT
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAATAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAATAAAAA
---------TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
TAAAGAAAAA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
2810
AAGATACAAC
AAGATACAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
---------AAGATGCAAC
AAGATGCAAC
AAGATACAAC
AAGATACAAC
AAGATACAAC
AAGATAGAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
---------AAGATGCAAC
---------AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
2820
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
---------CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
---------CCATAGAAAT
---------CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
2830
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
---------GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATTCAGA
GAAATTCAGA
GAAATTCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
---------GAAATCCAGA
---------GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
2840
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATCAG
TCATAATCAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
---------TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
---------TCATAATGAG
---------TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
2850
AGACT----AGACTCTTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
---------AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
---------AGACTCCTTT
---------AGATTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
2860
CTTTTGTTTT
GTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTT---------CTTTTGTTTT
C?TCTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTCTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
---------CTTTTGTTTT
---------CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
2870
CATTCAAAACATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
---------CATTCATAAA
CATTCATAAA
CAT-CATAAA
CATTCATAAA
CATTCATAAA
CATTCACAAA
CATTCAT--CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
---------CATTCATAAA
---------CATTCAT--CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
2880
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
155
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AAGATGCAAC
AAGATGCAAC
---------AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
AAGATGCAAC
CCATAGAAAT
CCATAGAAAT
---------CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
CCATAGAAAT
GAAATCCAGA
GAAATCCAGA
---ATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
GAAATCCAGA
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
TCATAATGAG
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
AGACTCCTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CTTTTGTTTT
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
CATTCATAAA
TCTTTTGTTT
-------------------------------------------------------------------------
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
2890
------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCATTCATAA
-------------------------------------------------------------------------
2900
GGCGGAAGCT
GGCGGAAGCT
GGGGTCCATT
GGCGGAAGCT
GGCG------GGCGGAAGC
-GGC------GGCGGAAGC
-GGCGGAAGC
-GGCGGAAG----------GGC------GGCGGAAGC
-GGCGCCGTT
-GGCGGAAGC
-GGCG-----GGC---------------GGCGGAAGC
-GGCGGAAGC
-GGCGG----GGCG?CAGC
----------GGCGGAAGC
------------GAAAGGC
-GGCGGAAGC
-GGCGGAAGC
-GGCGGAAGC
-GGCGGAAGC
-GGCGGAAGC
-GGCGGAAGC
-GGCG-----GGCGGAAGC
AGGCGC----GGCGGAAGC
-GGCGGAAGC
-GGCGG----GGCG-----GGCGGAAGC
-GGCGGAAGC
-GGCGGCAGC
-GGCG-----
2910
AAGA-----AAGT-----ACAAATGCGAAGAAAAAGA
---------TAGGAAAAAG
---------TAGGAAAAAG
TAGGAAAAAG
---------------------------TAGG-----CGG------TAAGAAAAAG
-----------GGAAAAAG
---------TAGG-----TAGG--------------T?GG--------------TAGGAAAAAG
---------G?-------TAGGAAAAAG
TAGGAAAAAG
TAGG-----TAGGAAAAAG
TAGGAAAAAG
TAGG--------------TAGGAAAAAG
---------TAGGAAAAAG
TAGG-----------------------TAGGAAAAAG
TAGG-----TAGG---------------
2920
---------------CCGT
------CCGT
ATCGA-CCGT
---------AATCGACCGT
---------T
AATCGACCGT
AATCGACCGT
------CCGT
------CCGT
---------------CCGT
---------AATCGACCGT
---------AATCGACCGT
---------------CCGT
------CCGT
---------------CCGT
---------AATCGACCGT
---------------CCGT
AATCGACCGT
AATCGACCGT
------CCGT
AGTCGACCGT
AATCGACCGT
------CCGT
---------AATCGACCGT
---------AATCGACCGT
------CCGT
------------------AATCGACCGT
------CCGT
-------------------
2930
-CGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
---------TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
---AGTATTC
TCGAGTATTC
---AGTATTC
TCGAGTATTC
-CGAGTATTC
TCGAGTATTC
--GAGTATTC
TCGAGTATTC
TCGAGTATTC
-CGAGTATTC
-CGAGTATTC
-------TTC
TCGAGTATTC
---------TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
-CGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
TCGAGTATTC
-----------GAG-ATTC
TCGAGTATTC
-CGAGTATTC
-CGAGTATTC
--GAGTATTC
2940
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
-ACCAAATTG
CACAAAATTG
CACCAAATTG
CACCAAATTG
CACAAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCA?ATTG
CACCAAATTG
CACCAAATTG
-------TTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CA-----TTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
-----AATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
CACCAAATTG
2950
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
CCTGATAAAA
2960
CTGAGAATAA
CTGAGAATAA
CGGAGAGTAA
CTGAGAGTAA
CTGAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTGAGAGTAA
CTGAGAGTAA
CTGAGAGTAA
CTGAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAT
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTGAGAGTAA
CTTAGAGTAT
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
CTGAGAGTAA
CTTAGAGTAA
CTTAGAGTAA
156
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
2970
T-AGGGGCAT
T-AGGGGCAT
TTAGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
ATAGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCCT
T-AGGGGCAT
T-AGGGGCAT
ATAGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCCT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
T-AGGGGCAT
2980
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
ATATATGTTA
2990
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
TGGAATACCC
3000
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
ATCTATATTG
3010
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
AATTGCGAAT
3020
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ATAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
ACAGAAATGA
3030
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATAGTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
TAAAATATTT
3040
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
TCTGATTGGA
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
3050
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
3060
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATAGGG
ATAAATATGG
ATAAATATGG
3070
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
3080
GAGACGAAAG
GAGACGAAAG
GAGACAAAAG
GAGACAAAAG
GAGACAAAAG
GAGACGAAAG
GAGACGAAAG
3090
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
3100
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
3110
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
TAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
3120
AAGACCCGTAAGACCCGTAAGACCCTTAAGACCCTTC
AAGACCCTTC
AAGACCCTTAAGACTCTT-
157
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATCAAA
CCAAATCAAA
CCAAATCAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATCAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATCAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATCAAA
CCAAATCAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
CCAAATAAAA
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
AAAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
ATAAATATGG
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GTCTCCGATA
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAG
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAG
GAGACGAAAA
GAGACGAAAG
GAGACGAAAA
GAGACGAAAA
GAGACGAAAA
GAGACGAAAG
GAGACGAAAG
GAGACGAAAG
GAGACGAAAA
GAGACGAAAA
GAGACGAAAG
GACACGAAAG
GAGACGAAAG
GAGACGAAAA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGAGAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGAGAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AAGATAAACA
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AAAAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAGAAAT
AACAAAAAAT
AACAAGAAAT
AACAAGAAAT
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAAATAGGTA
AAGACTCTTAAGACCCTTAAGACTCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCGTAAGACCCGTAAGACCCGTAAGACCCGTAAGACTCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGACCCTTAAGAC?CTTAAGACCCTTAAGACCCTTAAGACCCTT-
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
3130
---CAATATA
---CAATATA
---CAATATA
ATTCAATATA
ATTCAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
3140
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCCGTA
AGAATCCGTA
3150
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTAGATCTA
TTTATATCTA
TTTATATCTA
3160
CTAAATTCAA
CTAAATTCAA
CTAAATTCAA
CTAAATTCAA
CTAAATTCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATTCAA
CTAAATTCAA
CTAAATTCAA
3170
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCACA
CGGTTTCACA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
AGGTTTCGCA
AGGTTTCGCA
3180
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
3190
GAAAA----GAAAA----ATAAA----ATAAA----ATAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----ATAAATATAA
A--------A---------
3200
---------------------------------------------------------------------------------------------------------------------TGAAAGAAAA
-------------------
158
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
---CAATATA
AGAATCCGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCCATA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAATA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAGTA
AGAATCAATA
AGAATCAGTA
AGAATCAGTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
TTTATATCTA
CTAAATTCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATACAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
CTAAATGCAA
AGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
CGGTTTCGCA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
TAAAAAGAAA
ATAAATA-AA
GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA----GAAAA-----
TGAAAGAAAA
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
3210
TAAACAAATG
TAAACAAATG
TAAATAAATG
--------TA
--------TA
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
3220
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAACGG
AAAGAAACGG
AAAGAAACGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
3230
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAGGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
3240
AGA?AAGGTA
AGA?AAGGTA
AGA?AAGGTA
AGA?AAGGTA
AGA?AAGGTA
AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?AAGGTA
AGA?AAGGTA
AGA?AAGGTA
AGA?AAGGTA
AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?------
3250
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GGCGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
3260
----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCCCGGGCATCCCGGGCATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC-
3270
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAACTGAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
3280
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCCA?TCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTA-TCTCA
CCTAATCTCA
159
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
TAAACAAATG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAACGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
AAAGAAAGGG
Modiolastrum.lateritium
Modiola.caroliniana
Urocarpidium.albiflorum
Fuertesimalva.limensis
Fuertesimalva.jacens
Sphaeralcea.angustifolia
Sphaeralcea.cordobensis
Sphaeralcea.crispa
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.pedicularifolia
Nototriche.anthemidifolia
Malacothamnus chilensis
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.spiciformis
Tarasa.albertii
Tarasa.antofagastanaA
Tarasa.antofagastanaC
Tarasa.antofagastanaB
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonellii
3290
AGACACAAAG
AGACACAAAG
AGACACAAAG
AT?CGCAGA?
AAACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
-GACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
G
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
GAAAGGAGGG
AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?-----AGA?------
160
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACAGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
GACGGAAGGG
----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC----CATCC-
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAG-GAGAT
TAAC-GGGAT
TAAC-GAGAT
TAAC-GAGAT
TAAC-GAGAT
TAAG-GAGAT
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
CCTAATCTCA
Tarasa.operculata
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.tarapacana
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea
Tarasa.trisecta
Tarasa.urbaniana
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
AGACACAAAG
G
G
G
G
G
G
G
G
G
161
Appendix C. Tarasa and outgroups ITS alignment
ITS1 (1-304), 5.8S (305-468), ITS2 (469-703)
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
10
20
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCT-GCAGAA
CCTAGCAGAA
CCT-GCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
30
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCCGTG
-CGACCTGTG
-YG-CCTGTG
ACGACCTGTG
-CGACCCGTG
-CGACCCGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCCGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCCGCG
-CGACCCGTG
-CGACCTGTG
-TGACCCGTG
-CGACCCGTG
-CGACCTGTG
-CGACCCGTG
-CGACCCGTG
-CGACCTGTG
40
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACATGATAT
AACATGATAT
AACGTGATAT
AACATGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
162
50
CAAACAACTC
CAAACAACTC
AAAACAACTC
CAAACAACTA
CAAACAACTA
CAAACAACTC
CAAACAACTC
CAAACAACTC
CAAACAACTC
CAAACAACTC
CAAACAACCC
CAAACAACCC
CAAACAACCC
CATACAACTC
CAAACAACTA
CAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
AAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
TAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACCC
CAAACAACTA
CAAACAACCC
AAAACAACTC
CAAACAACTC
CAAACAACTC
CAAACAACCC
60
AACGAGGGGG
AACGAGGGGG
AACGAGGGGG
AACGAGGGGG
AACGAGGGGG
CACGAGGGGG
CACGAGGGGG
CACGAGGGGG
CACGAGGGGG
CACGAGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGAGGGGG
CACGGGGGGG
CGCGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CGCGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGAGGGGG
CACGAGGGGG
CACGGGGGGG
70
-CGTAGATGC
-CGTAGATGC
-CGTAAATGC
-CGCGGATGC
-CGCGGATGC
-TGCGGATGC
-CGCGGATGC
-CGCGGATGC
-TGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGACGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGACGC
-CGCGGATGC
-CGCGGATGC
-TGTGGATGC
-CGCGGATGC
-CGCGGGTGC
-CGTGGATGC
GCGCGGATGC
-CGCGGATGC
-CGTGGATGC
-YGCGGAYGC
-CGCGGATGC
-CGTGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-YGCGGATGC
80
ATTCTTGCAC
ATCCTTGCAC
ATCATTGCAC
ATCCTTGCAC
ATCCTTGCAC
ATCTTTGCCC
ATCCTTGCCC
ATCYTTGCCC
ATCTTTGCCC
ATCCTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCCTTGCCC
ATCCTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCCTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCCTTGCCC
ATCCTTGCCC
ATCTTTGCCC
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACTTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
TCGAAACCTG
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
CCTAGCAGAA
90
100
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
CCAAACCCCC
CCAAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-GAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AGACCCCC
C-AAACCCCC
C-AAACCCCC
C-GAACCCCC
TCGATGCCTT
TCGATGCCTT
TTGATGTCTT
TCGATGCCTT
TCGATGCCTT
TCGTTGCCTT
TTGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGATGCCTT
TCGATGCCTT
TCGGTGCCTT
CCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCCGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGCTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGATGCCTT
-CGACCTGTG
-CGACCTGTG
-CGACCCGCG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCCGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-CGACCTGTG
-TGACCTGTG
-CGACCTGTG
-TGACCTGTG
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
AACATGATAT
AACGTGATAT
AACGTGATAT
AACGTGATAT
110
GG---TGTGC
GG---TGTGC
AG---TTTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
TG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
120
TTGGCTTTGT
TTGGCT---TTGGCTTCGC
TTGGCCTTTC
TTGGCCTTTC
TTGCCCTTGC
TTGCCTTTGC
TTGCCTTTGC
TTGCCTTTGC
TTGCCTTTGC
TTGTCTTTGT
TTGCCTTTGT
TTGTCTTTGT
TTGCCTTTGC
TTGCCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGCCTTTGC
163
AAAACAACTC
AAAACAACCC
CAAACAACCC
AAAACAACCT
CAAACAACCT
CAAACAACCC
CAAACAACCC
CAAACAACTC
AAAACAACCC
CAAACAACCC
AAAACAACCC
CAAACAACCC
CAAACAACCT
CAAACAACCT
CAAACAACCC
CAAACAACCC
130
CAC-A----------------------ACC-ATCCGC
ACC-ATCCGC
CCC-ATCCAC
CCC-ATCCAC
CCC-ATCCAC
CCC-ATCCAC
CCC-ATCCAC
ACC-ATCCTC
ACC-ATCCTC
ACC-ATCCTC
CCC-ATCCGC
CCC-ATCCGY
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
GCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCGC
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGAGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
CACGGGGGGG
140
---------------------------TCTT---GGG
TCTT---GGG
TCTC---GGG
TCTTTTTGGG
TCTC---GGG
TCTC---GAG
TCTTTTTGGG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---TGG
TCTC---GCG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTT---GGG
-CGCGGATGC
-CGAGGATGC
-CGCGGATGC
GCACGGATGC
-CGTGGATGC
-CGCGGATGC
-TGCGGATGC
-TGCGGATGC
-TGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGCGGATGC
-CGTGGATGC
-CACGGATGC
-YGCGGATGC
-CGCGGATGC
150
--------------------------AT
TGGGTGTGAT
CGGGTGTGAT
CGGTTGAGTT
CGGTTGARAT
CGGTTGAGAT
CGGTTGAGTT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGTT
CGGATGAGAT
TGGTTGAGTT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGAT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGAT
CGGTTGAGTT
CGGTTGAGTT
CGGTTGAGAT
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
AACTTTGCTC
ATCTTTGCCC
ATCTTTGCCC
ATCCTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGCCC
ATCTTTGTCC
ATCTTTGCCC
ATCTTTGCCC
ATC-TTGCCC
ATCTTTGCCC
160
-----------------TT
GCGAAGTCTT
GGCAGGTCTA
GCCAGGTCTT
GCAAGGTCTT
GCAAGGTCTT
GCAAGGTCTT
GCAATGTCTC
GCAAGGTCTT
GCAAGCT--T
GCAAG--CTT
GCAAG-TCTT
GCCAGGTCTT
GCAAGGTCTT
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTT
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCTT
GCAAGGTCTA
GCAAGGTCTA
GCAAGGTCCT
GCAAGGTCTA
GYAAGGTCTA
GCAAGGTCTT
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AGACCCCC
C-GAACCCCC
C-GAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-AAACCCCC
C-GAACCCCC
C-GAACCCCC
C-AAACCCCC
C-AAACCCCC
170
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
------CTCC
GTCACACTCC
GTCAC--TCC
GTCAC--TCC
GTCAC--TCC
GTCACACTCC
GTCACACTCC
GTCACACTCC
GTCACACTCC
GTCACACTCC
GTC---CCCT
GTC---CCCT
GTC---CACT
GTCACACTTC
GTCACATTCT
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GTC----TCC
GCC----TCC
TCGGTGCCTT
TCGGTGCCTT
TCGATGCCTT
TCGATGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGATGCTTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
TCGGTGCCTT
180
AAGGCAAA-AAGGCAAA-AAGGCAAAAA
GAGGCAAAAGAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAGAGGCAAAAGAGGCAAAAGAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAA-
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---CGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
GG---TGTGC
TTGTCTTTGC
TTGTCTTTGC
TTGCCTTTAC
TTGCCTTTAC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGCCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGCCTTTAC
TTGTCTTTGC
TTGCTTTTGC
TTGTCTTTGC
TGGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
TTGTCTTTGC
190
TGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
CGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
CAAACAACCC
CGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
200
CCGGCGCGAA
CCGGCGCGAA
CCGGCGCGAA
CCGGCGCGAA
CCGGCGCGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGCGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
164
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCGC
CCC-ATCCGC
CCC-ATCCAC
CCC-ATCCTC
CCCCATCCTC
GCC-ATCCTC
CTC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCGC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
CTC-ATCCTC
CCC-ATCCTC
CCC-ATCCTC
210
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTACGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTACGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TCTC---GAG
TCTC---GAG
TCTC---GGG
TCTC---GGG
TCTC---GAG
TCTA---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GGG
TCTC---GGG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTC---GAG
TCTT---GAG
TCTC---GAG
220
GAATAAAAAA
GAATAAAAAA
GAATTAAAAA
GAATAAAAAA
GAATAAAAAA
GATTTGAAAA
GATTTGAAAA
GATTTGAAAA
GATTTTAAAA
GATTTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTAAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTAAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
CGGTTGAGTT
CGGATGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGATGAGAT
CGGATGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGTT
CGGTTGGGAT
CGGTTGAGAT
CGGATGAGAT
CGGTTGAGTT
CGGATGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGAT
CGGTTGAGTT
230
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
-TGGAAAGAG
-TGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
-TGGAAAGAG
-TGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
GCAAGGTCTG
GCAAGGTCCT
GCCAGGTCTT
GCCAGGTCTT
GCAACGTCTT
GCAAGGTCCT
GCAAGGTCTT
GCAAGGTCCT
GCAAGGTCTT
GCAAGGTCTT
GCAAGGTCTA
GCAACGTCTT
GCCAGGTCTT
GCAAGGTCTT
GCAAGGTGTA
GCAAGGTCTT
GCGAGGCCTT
GCAAGGTCTT
GCAAGGTCTT
GCAAGGCCTT
GCAAGGTCTA
240
TGCACGTCTG
TGCACGTCTG
TGCACGTCTG
TGCACGTCAG
TGCACGTCTG
TGCACGTTTG
TGCACGTTTG
TGCACGTTTG
TGCACGTTTG
TGCACGTTTG
TGCATGT-TG
TGCATGT-TG
TGCATGT-TG
TGCACGTTTG
TGCACGTTTG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TA
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCGCGT-TG
TGCGTGT-TG
TGCACGT-TG
TGCACGT-TG
TGCATGT-TA
TGCACGT-TG
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
GTC----TCC
GTC----TCC
GTCACACTAT
GTC----TCC
GTC----TCC
GTCACACTTC
GTCACACTTC
GTC----TCC
GTC----TCC
GTC----TCC
GCC----TCC
GTCACATTCC
GTCACATTCC
GTC----TCC
GTC----TCC
GTCACACTTC
GTC----TCC
GTC----TCC
GTC----TCC
CTC----TCC
GTCACATTCC
GTCACATTCC
GTC----TCC
GTC----TCC
250
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
CTGCCGTCST
CTGCCGTCGT
CTGTCGTCAT
CTGTCGCCGT
CTGTCGCCGT
CTGTCGTCGT
CTGTCGCCAT
CTGTTGCCAT
CTGTCGTCGT
CTGTCGCCAT
CTGTTGCCGT
CTGTTGCCGT
CTGTTGCCGT
CTGTCGACGT
CTGTCGCCGT
CTGTTGCCAT
TTGTTGCCAT
TTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCGT
AAGGCAAAAAAGGCTAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCCAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAAAAGGCAAAA260
CCCGTTCGCG
CCCGTTCGCG
CCCGTTCGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
TGAACAACCC
TGAACAACCC
CAAACAACCC
TGAACAACCC
TGAACAACCC
CGAACAACCC
CGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
CGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGAACAACCC
TGATCAACCC
CGAACAACCC
TCAACAACCC
TGAACAACCC
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGCGAA
CCGGCGCGAA
CCGGCGCGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGCGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
CCGGCGTGAA
270
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGT
GTGTCTGTGT
GTGTCTGTGC
GTGTCTGTGT
GTGTCTGTGT
GTGTCTGTGC
GTGTCTGTGT
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTATGTGC
GTGTATGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGACTGAGC
280
GGTAGTGACG
GGTAGTGACG
GGCAGCAACG
GGCAGTGACG
GGCAGTGGCG
GGCAGTGACG
GGCAGTGACG
GGCAGTGACG
GGCAGTGACG
GGCAGTGACG
GACAGTGATGACAGTGATGACAGTGATGGCAGTGACG
GGCAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
165
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
TTGCGCCAAG
290
TTGTTACTTT
TTGTTACTTT
CTGTTACTTT
CTGTTACTTT
CTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TT---ACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
GAATTGAAAA
GAATTGAAAA
GAATTAAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTAAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTAAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
GAATTGAAAA
300
TGTCGTCAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGATA
TGTCGTGATA
TGTCGTGAAA
TGTCGTGATA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
-TGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
ATGAAAAGAG
-TGAAAAGAG
310
CTACAAAACG
CTACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
TGCGCGT-TG
TGCACGT-TG
TGCACGTTTG
TGCGCGT-TG
TGCACGT-TG
TGCACGTTTG
TGCACGTTTG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TA
TGCATGT-TG
TGCACGTTTG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
TGCACTT-TG
TGCACGT-TG
TGCACGT-TG
TGCACGT-TG
320
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
CTGTTGCCAT
CTGTTGCCAT
TTGTTGCCGT
CTGTTGCCAT
CTGTTGCCAT
CTGTCGCCRT
CTGTTGCCAT
CTGTTGCCAT
CTGTCAACGT
CTGTCAACGT
CTGTTGCCGT
CTGTTGCCAT
CTGTTGCCAT
TTGTTGCCGT
TTGTCGTCGT
CTGTCGTCGT
CTGTTGCCAT
CTGTTGCCGT
CTGTCAACGT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTTGCCAT
CTGTCGTTGT
TTGTCGTCGC
CTGTTGCCAT
CTGTTGCCAT
330
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
CCCGTACGCG
340
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
GTGTCTGTGC
GTGTCTGTGC
GTGTATGTGC
GTGTCTGTGC
GTGTMTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGT
GTGTCTGTGT
GTGACAGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTATGTGC
GTGTCTGTGC
GTGTTTGTAC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGT
GTGTCTGTGC
GTGTTTGTGC
GTGTTTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTCTGTGC
GTGTTTGTGC
GTGTCTGTGC
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GGCAGCGATG
GACAGTGATG
GACAGTGATG
GGCAGTGACG
GGCAGTGACG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GGCAGTGACG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
GACAGTGATG
350
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
360
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGCAGCGA
AACGTAGCGA
AACGTAGCGA
AACGCAGCGA
AACGTAGCGA
AACGCAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
166
TTGTTACTTT
TTGTTACTTT
ATGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
CTGTTACTTT
TT---ACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
TTGTTACTTT
370
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTAAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
-GTCGTGAAA
TGTCGTGAAA
TGTCGTGAAA
380
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
ATACAAAACG
ATACAAAACG
ATAAAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATAAAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
TTACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
ATACAAAACG
390
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
ACTNTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
ACTCTCGGCA
400
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGT-AACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
ACGGATATCT
410
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
CGGCTCTCGC
420
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
ATCGATGAAG
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGCAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
AACGTAGCGA
430
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
440
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
167
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
AATGCGATAC
450
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
TGAGGGCACG
TGAGGGCACG
CGAGGGCACG
TGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
TTGGTGTGAA
460
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
TTGCAGAATC
470
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
CCGTGAACCA
480
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTTGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
TCGAGTCTTT
490
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
-AATCAAATC
-AATCAAATC
-AATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAGCC
--ATCAAGCC
--ATCAAACC
--ATCAAGCC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAAAC
--ATCAAACC
--ATCAAACC
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
GAACGCAAGT
500
-TCAGCCCTC
-TCAGCCCTC
CTCAGTCCTC
CTGAGCCCTC
CTTATCCCTC
ATTAGTCCTC
ATTAGTCTTY
ATTAGTCTTC
ATTAGTCCTC
ATTAGTCTTC
ATTAGCCCTC
ATTATCCCTC
ATTAGCCCTC
CTAAGCCCTT
ATTAGCCCTC
ATTAGCCCTC
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
TGCGCCCCAA
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
GCCATTAGGC
510
CGGCTGCG-T
CGGCTGCG-T
GGGCTGCGGT
GGGCTACGGT
GGGCTACGGT
GGGCTACGGT
GGGCTATGGT
GGGCTATGGT
GGGCTATGGT
GGGCTATGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCTACGGT
GTGCTACGGT
GGGCAACGGT
520
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TCT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGY
TTAAC--TGT
TTAAC--TGT
TTAAY--TGT
TGAAT--TGT
TGAAT--TGT
TGAGC--TGT
168
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
CGAGGGCACG
530
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAC
GGGCGGAAAC
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
TCTGCCTGGG
540
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGTCTCCCG
TGGTCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
TGTCACGCAT
550
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTAACC
TGCGCTCACC
TGCGCTCACC
CGTCGCCCCC
CGTCGCCCCCGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCAC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCAC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
TGTCGCCCCCGTCGCCCCC
CGTCGCCCCC
CGTCGCCCCC
560
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGCT
GCTCGCGGTT
GCTCGCGGTT
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATTAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCCAACC
--CTCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAACCC
--ATCAACCC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAAACC
--ATCAACCC
--ATCAACCC
--ATCAAACC
--ATCAAACC
570
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GTTAGCCCTC
GTTAGCCCTC
GTTAGCCCTC
ATTAGCCCTC
ATTAGCCTTT
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCCC
TTTAGCCCTC
TTTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
RTTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
CTAAGCCCTT
CTWAGCCCTT
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
TTTAGCCCTC
ATTAGCCCTC
CTAAGCCCTT
ATTAGCCCTC
TTTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTAGCCCTC
ATTTGCCCTC
ATTAGCCCTC
TTTAGCCCTC
580
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCTTCG
CGAGTCCTCG
MGAGTCCTCG
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGAAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAGCGGT
GGGCAACGGT
GGGCAACGGT
GTGCTATGGT
GGGCAACGGT
GGGCAACGGT
GGGCTACGGT
GGGCTATGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAGCGGT
GGGCAACAGT
GGGCAATAGT
GGGCAACGGT
GGGCAACGGT
GGGCTACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAACGGT
GGGCAATAGT
GGGCAACAGT
GGGCAACGGT
GGGCAACGGT
TGAGC--TGT
TGAGC--TGT
TGAAC--TGT
TGAGC--TGT
TGAAT--TGT
TGAAC--TGT
TGAGT--TGT
TGAGT--TGT
TGAAA--TGT
TGAAC--TGT
TGAAC--TGT
TGAAT--TGT
TGAGT--TGT
TGAGC--TGT
TGATT--TGT
TGAGT--TGT
TGAAC--TGT
TGAAT--TGT
TGAAT--TGT
TGAAC--TGT
TGAAC--TGT
GGAAC--TGT
TGAAT--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAT--TGT
TGAAC--TGT
TGAAC--TGT
GGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAC--TGT
TGAAY--TGT
TGAAC--TGT
590
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATCAAGT
GCGAKKAAGT
GCGATGAAGT
GCGATSAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
600
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GTCGCGACAA
GCCGCGACAA
GCYGCGACAA
GCCRCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GYCGCGACAA
GCCGCGACAA
169
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
GGGCGGAAAT
610
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
TGGCCTCCCG
620
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGTGCTCATC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGCGCTCACC
TGTGCTCACC
TGCGCTCACC
TGCGCTCACC
630
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
AGCCTCGTTC
AGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCACGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCCCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCCCGCGGTT
GCTCACGGTT
GCTCACGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
GCTCGCGGTT
CCTCGCGGTT
GCTCACGGTT
GCTCACGGTT
GCTCGCGGTT
GCTCGCGGTT
640
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
GGAGTCGTGT
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAGAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
GGCCTAAAAT
650
Fuertesimalva.limensis
Fuertesimalva.jacens
Urocarpidium.albiflorum
Modiola.caroliniana
Modiolastrum.lateritium
Sphaeralcea.angustifolia
Sphaeralcea.crispa
Sphaeralcea.cordobensis
Sphaeralcea.wrightii
Sphaeralcea.philippiana
GC-GCTCGTT
GC-GCTCGTT
GC-TCTCGTT
GT-GCTCGTT
GC-GCTCGTT
GC-ACTCGTC
YC-ACTCGTY
KC-ACTMGYT
GC-ACTCGTC
TC-ACTCGYT
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAATCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
CGAGTCCTCG
TGAGTCCTCG
660
GATT-AGGAT
GATT-AGGAT
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GCGATGAAGT
GCGATGAAGC
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCGATGAAGT
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCACGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCACGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GTCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
GCCGCGACAA
670
C-TCTGACCC
C-TCTGACCC
C-TCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
680
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
170
TCGGTGGGAT
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAT
TCGGTGGGAT
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAT
TCGGTGGGAT
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
TCGGTGGGAA
690
TCGCAACRTC
TCGCAACGTC
TCGCAACGTC
CCGCAAAGTC
CCGCAACGTC
TTGCAACGTC
TCTCAATGTC
TCTCAATGTC
TTGCAACGTC
TCTCAATGTC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTTGAGC
YGCTTTTAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTTCAGC
TGCTTTTAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTTTAGC
TGCTTTGAGC
TGCTTTGAGC
TGCTTATAGC
TGCTTTGAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTGAGC
TGCTTCTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTGAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTTAGC
TGCTTTGAGC
TGCCTCGTAC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGACTCTTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTT
TGCCTCGTTT
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTC
TGCCTCGTTT
TGCCTCGTTT
TGCCTCGTTC
TGCCTCGTTC
700
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
AATGCTCGCA
GATGCTCGCA
GATGCTCGCA
AATGCTCGCA
GATGCTCGCW
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
GAAGTCGTGT
GGAGTCGTGT
GTAGTCGTGC
GTAGTCGTGC
GTAGTCGTGC
GTAGTCGTGT
GTAGTCGTGC
GGAGTCGTGC
GTAGTCGTGC
GTAGTCGTGT
GTAGTCGTGC
GGAGTCGTGT
GTAGTCGTGT
GTAGTCGTGT
GGAGTCGTGC
GTAGTCGTGC
GTAGTCGTGC
GGAGTCGTGT
GTAGTCGTGC
GGAGTCGTGC
GAAGTCGTGT
GAAGTCGTGT
GGAGTCGTGC
GGAGTCGTGC
GTAGTCGTGC
GGAGTCGTGC
GTAGTCGTGC
GTAGTCGTGT
GTAGTCGTGT
GGAGTCGTGT
GAAGTCGTGT
GGAGTCGTGC
GTAGTCGTGT
GTAGTCGTGT
GGAGTCGTGT
GTAGTCGTGT
GTAGTCGTGC
GGAGTCGTGT
GTAGTCGTGT
Nototriche.flabellata
Nototriche.anthemidifolia
Nototriche.pedicularifolia
Malacothamnus.chilensis
Tarasa.albertii
Tarasa.antofagastanaB
Tarasa.antofagastanaC1
Tarasa.antofagastanaA2
Tarasa.antofagastanaC2
Tarasa.antofagastanaA1
Tarasa.capitata
Tarasa.cardenasii
Tarasa.cerratei1
Tarasa.cerratei2
Tarasa.congestiflora
Tarasa.corrugata
Tarasa.geranioides1
Tarasa.geranioides2
Tarasa.geranioides3
Tarasa.heterophylla
Tarasa.humilis
Tarasa.latearistata1
Tarasa.latearistata2
Tarasa.machupicchensis
Tarasa.mandonii
Tarasa.marinii
Tarasa.meyeri
Tarasa.nototrichoides
Tarasa.odonelli
Tarasa.operculata1
Tarasa.operculata2
Tarasa.pediculata
Tarasa.rhombifolia
Tarasa.spiciformis
Tarasa.tarapacana1
Tarasa.tarapacana2
Tarasa.tenella
Tarasa.tenuis
Tarasa.thyrsoidea2
Tarasa.thyrsoidea1
Tarasa.trisecta
Tarasa.urbaniana
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GC-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GGTCGTT
GT-GCTCGTT
TC-GCTCGTT
GT-GGTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
AT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GT-GCTCGTT
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATTT-GGAC
GATT-AGGAC
GATTT-GGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATTT-GGAC
GATTT-GGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATTTAGGAC
GATTT-GGAC
GATT-AGGAC
GATT-AGGAC
GATTT-GGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATTTAGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GATT-AGGAC
GAAT-AGGAC
GATT-AGGAC
GATT-AGGAC
GAAT-AGGAC
GATT-AGGAC
CCTTTGACCC
CCTTTGACCC
CCTTTGACCC
CCTCTGACCC
ACTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTTTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
ACTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCCGACCC
TCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTTTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGGCCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTCTGACCC
CCTTTGACCC
CCTCTGACCC
CCTCTGACCC
CCTTTGACCC
CCTTTGACCC
TTTTT-GGSA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTCA-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTTTGGCA
TTTTT-GGCA
TTTTT-GGCA
TTTCA-GGCA
TTTT--GGCA
TTTT--GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTTT-GGCA
TTTT--GGCA
TTTT--GGCA
TTTTT-GGCA
TTTTT-GGCA
171
TCACAAAGTA
TCACAAAGTA
TCCCAAAGTA
TCACAATGTT
TCGCAACGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACTAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAATGTC
TCACAAAGTC
TCACAAAGTC
TCGCAACGTC
TCACAAAGTC
TCACAAAGTC
TCGCAATGTT
TCGCAATGTT
TCACTAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAATGTC
TCACAACGTA
TCACAACGTA
TCACAAAGTC
TCACTAAGTC
TCGCAATGTT
TCACAAAGTC
TCACAAAGTC
TCACAAAGTC
TCACAATGTC
TCACAACGTA
TCACAACGTA
TCACAAWGTC
TCACAAAGTC
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
GATGCTCGCA
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TCG
TTG
TCG
TCG
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Vita
Jennifer Alane Tate was born April 25, 1972 in Charleston, Illinois to Ann
Marie and Gary Wayne Tate. She graduated valedictorian from Auburn High
School (Auburn, Illinois) in 1990 and earned a B.S. in Plant Biology from the
University of Illinois at Urbana-Champaign in 1994. During her senior year, she
studied abroad at the University College of North Wales at Bangor.
After
graduating, she worked for two years at the Illinois Natural History Survey as a
herbarium assistant. She began the Ph.D. program at the University of Texas at
Austin in August 1996.
Permanent address: 2300 Broadmoor Drive, Apt. 47, Bryan, TX 77802
This dissertation was typed by the author.
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Jennifer Tate