Abstract
Immunocytochemical analysis of meiotic proteins on mouse chromosome spreads is one method of choice to study prophase I chromosome organization and homologous recombination. In recent decades, the development of microscopic approaches led to the production of a large number of images that monitor fluorescent proteins, defined as fluorescent objects, and a major challenge facing the community is the deep analysis of these fluorescent objects (measurement of object length, intensity, distance between objects, as well as foci identification, counting, and colocalization). We propose a set of tools designed from the macro language of the widely used image analysis software ImageJ (Schindelin et al., Nat Methods 9: 676–682, 2012), embedded in the “MeiQuant” macro, which are specifically designed for analyzing objects in the field of meiosis. Our aim is to propose a unified evolutive common tool for image analysis, with a specific focus on mouse prophase I meiotic events.
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Acknowledgments
We thank the Montpellier Resources Imagerie (MRI), a member of the national infrastructure France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04, “Investments for the future”) for microscopy. We acknowledge the CNRS INSERM ATIP-Avenir 2017 program and ANR CONDENSin3R (ANR-20-CE12-0016-02) that support the work performed in TR lab. This work was supported by the Agence Nationale de la Recherche ANR FIRE (ANR-17-426 CE12-0015) and by the Ligue contre le Cancer (Comités départementaux de l’Hérault et du Gard) to F.B. L.D.T. is funded by a PhD fellowship from Université Montpellier, UM, ED CBS2. A.Z. was funded by the ANR FIRE and a PhD fellowship (FDT202106012805) from the Fondation pour la Recherche Médicale (FRM).
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Cau, J., Toe, L.D., Zainu, A., Baudat, F., Robert, T. (2024). “MeiQuant”: An Integrated Tool for Analyzing Meiotic Prophase I Spread Images. In: Barchi, M., De Felici, M. (eds) Germ Cell Development. Methods in Molecular Biology, vol 2770. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3698-5_17
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DOI: https://doi.org/10.1007/978-1-0716-3698-5_17
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