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Genotype-Specific Features of Cold-Induced Sweetening Process Regulation in Potato Varieties Nikulinsky, Symfonia, and Nevsky

  • BIOINFORMATICS, BIOENGINEERING, AND BIOTECHNOLOGY
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Abstract—In this study, we performed expression analysis of genes associated with cold-induced sweetening in potato tubers: vacuolar invertase (Pain-1), sucrose synthase (SUS4), and invertase inhibitor (InvInh2). Potato varieties Nikulinsky, Symfonia, and Nevsky were used. All three varieties were found to accumulate sugars at low temperatures; the maximum accumulation of reducing sugars was observed at 4°C. It was found that the expression pattern of genes associated with cold-induced sweetening differs depending on the variety and storage duration. The increased expression of vacuolar invertase and its inhibitor is more pronounced at the beginning of storage period, whereas the increased expression of sucrose synthase is more pronounced after 3 months of storage. At early storage periods, high expression of invertase and low expression of inhibitor is observed in the Dutch variety Symfonia, and vice versa in the Russian varieties Nikulinsky and Nevsky. The involvement of the studied genes in the process of cold-induced sweetening is discussed.

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ACKNOWLEDGMENTS

We are grateful to S.V. Gerasimova and D.A. Afonnikov for their help in conducting the study and writing the article.

Funding

The analysis of the sugar content during storage was performed within the framework of the subprogram of the Complex Science and Technology Program “Development of Selection and Seed Production of Potatoes in the Russian Federation,” project no. FWNR-2019-0012. The development of primers and analysis of gene expression was carried out as part of the budgetary project no. FWNR-2022-0017.

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Authors and Affiliations

  1. Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090, Novosibirsk, Russia

    A. A. Egorova, N. E. Kostina, D. D. Kuvaeva, A. B. Shcherban, S. M. Ibragimova & A. V. Kochetov

  2. Kurchatov Genomic Center of the Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, 630090, Novosibirsk, Russia

    A. A. Egorova, I. A. Saboiev, A. B. Shcherban, S. M. Ibragimova & E. A. Salina

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  1. A. A. Egorova
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  2. I. A. Saboiev
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  4. D. D. Kuvaeva
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Correspondence to A. A. Egorova.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Egorova, A.A., Saboiev, I.A., Kostina, N.E. et al. Genotype-Specific Features of Cold-Induced Sweetening Process Regulation in Potato Varieties Nikulinsky, Symfonia, and Nevsky. Mol Biol 57, 193–203 (2023). https://doi.org/10.1134/S0026893323020061

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