Skip to main content

Advertisement

SpringerLink
Log in

Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

  • Short Communication
  • Published:
Brazilian Journal of Botany Aims and scope Submit manuscript

Abstract

Aechmea distichantha Lem. is a Bromeliaceae species with wide geographic distribution; it is found in Atlantic Forest, “Cerrado” and “Chaco” ecoregions from Tropical to Subtropical areas in Brazil, Bolivia, Paraguay and Argentina. The species has a great ornamental value and is currently threatened by its predatory exploration and by habitat loss. The lack of suitable molecular markers hampers research on its genetic diversity, which could contribute to the design of conservation plans and to demographic and phylogeographic studies of the species. Here, we tested the cross-amplification of 43 nuclear microsatellite markers, originally developed for other bromeliad species. We obtained high cross-amplification indices (37 SSR primers, 86%) and polymorphism (17 SSR primers, 46%). We then used ten SSR loci to genotype individuals from three populations of A. distichantha. The observed and expected heterozygosity per locus in the A. distichantha populations ranged from 0.182 to 0.735 and 0.297 to 0.830, respectively. These loci showed sufficient variability to be used in future the studies of genetic diversity, genetic structure and phylogeography of A. distichantha to understand its evolutionary history during its dispersal, colonization and adaptation to different ecoregions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Abondanza BF (2012) Revisão taxonômica do complexo Aechmea coelestis (K. Koch) E. Morren (Bromeliaceae). Dissertation, Instituto de Botânica de São Paulo, São Paulo

  • Aoki-Gonçalves F, Louzada RB, Souza LM, Palma-Silva C (2014) Microsatellite loci for Orthophytum ophiuroides (Bromelioideae, Bromeliaceae) species adapted to neotropical rock outcrops. Appl Plant Sci 2:1–4

    Article  Google Scholar 

  • Barbará T, Martinelli G, Fay MF, Mayo SJ, Lexer C (2007a) Population differentiation and species cohesion in two closely related plants adapted to neotropical high-altitude ‘inselbergs’, Alcantarea imperialis and Alcantarea geniculata (Bromeliaceae). Mol Ecol 16:1981–1992

    Article  PubMed  Google Scholar 

  • Barbará T, Palma-Silva C, Paggi GM, Bered F, Fay MF, Lexer C (2007b) Cross-species transfer of nuclear microsatellite markers: potential and limitations. Mol Ecol 16:3759–3767

    Article  PubMed  Google Scholar 

  • Barbará T, Martinelli G, Palma-Silva C, Fay MF, Mayo SJ, Lexer C (2009) Genetic relationships and variation in reproductive strategies in four closely related bromeliads adapted to neotropical ‘inselbergs’: Alcantarea glaziouana, A. regina, A. geniculata and A. imperialis (Bromeliaceae). Ann Bot 103:65–77

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Benzing DH (2000) Bromeliaceae: profile of an adaptive radiation. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Boneh L, Kuperus P, Van Tenderem PH (2003) Microsatellites in the bromeliads Tillandsia fasciculata and Guzmania monostachya. Mol Ecol Notes 3:302–303

    Article  CAS  Google Scholar 

  • Carvalho FMV, De Marco Jr P, Ferreira Junior LG (2009) The “Cerrado” into-pieces: habitat fragmentation as a function of landscape use in the savannas of central Brazil. Biol Conserv 142:1392–1403

    Article  Google Scholar 

  • Chaves CJN, Aoki-Gonçalves F, Leal BSS, Rossatto R, Palma-Silva C (2018) Transferability of nuclear microsatellite markers to the atmospheric bromeliads Tillandsia recurvata and T. aeranthos (Bromeliaceae). Braz J Bot 41(4):931–935

    Article  Google Scholar 

  • Dieringer D, Schlötterer C (2003) Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Ferreira DMC, Neri J, Palma-Silva C, Pinangé DS, Benko-Iseppon AM, Louzada RB (2017) Cross-amplification of nuclear microsatellite markers in two species of Cryptanthus Otto & A. Dietr. (Bromeliaceae). Rev Bras Bot 40:475–480

    Article  Google Scholar 

  • Freire RM, Barberis IM, Vesprini JL (2018) Reproductive traits and floral visitors of Aechmea distichantha plants growing in different habitats of a South American xerophytic forest. Rodriguésia 69(2):385–396

    Article  Google Scholar 

  • Godoy FMR, Lenzi M, Ferreira BHS, Silva LV, Zanella CM, Paggi GM (2018) High genetic diversity and moderate genetic structure in the self-incompatible, clonal Bromelia hieronymi (Bromeliaceae). Bot J Linn Soc 187:672–688

    Article  Google Scholar 

  • Goetze M, Louzada RB, Wanderley MGL, Souza LM, Bered F, Palma-Silva C (2013) Development of microsatellite markers for genetic diversity analysis of Aechmea caudata (Bromeliaceae) and cross-species amplification in other bromeliads. Biochem Syst Ecol 48:194–198

    Article  CAS  Google Scholar 

  • Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Article  Google Scholar 

  • Hedrick F (2005) A standardized genetic differentiation measured. Evolution 59:1633–1638

    Article  CAS  PubMed  Google Scholar 

  • Lavor P, van den Berg C, Jacobi CM, Carmo FF, Versieux LM (2014) Population genetics of the endemic and endangered Vriesea minarum (Bromeliaceae) in the Iron Quadrangle, Espinhaço Range. Am J Bot 101:1167–1175

    Article  PubMed  Google Scholar 

  • Luther HE (2012) An alphabetical list of bromeliad binomials. Marie Selby Botanical Gardens and Bromeliad Society International, Maddison

    Google Scholar 

  • Martinelli G, Vieira CM, Gonzalez M, Leitman P, Piratininga A, Costa AF, Forzza RC (2008) Bromeliaceae da Mata Atlântica Brasileira: lista de espécies, distribuição e conservação. Rodriguésia 59:209–258

    Article  Google Scholar 

  • Meireles JE, Manos PS (2018) Pervasive migration across rainforest and sandy coastal plain Aechmea nudicaulis (Bromeliaceae) populations despite contrasting environmental conditions. Mol Ecol 27:1261–1272

    Article  CAS  PubMed  Google Scholar 

  • Myers N, Mittermeier RA, Mittermeier CG, Da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858

    Article  CAS  Google Scholar 

  • Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Nat Acad Sc 70:3321–3323

    Article  CAS  Google Scholar 

  • Neri J, Nazareno AG, Wendt T, Palma-Silva C (2015) Development and characterization of microsatellite markers for Vriesea simplex (Bromeliaceae) and cross-amplification in other species of Bromeliaceae. Biochem Syst Ecol 58:34–37

    Article  CAS  Google Scholar 

  • Paggi GM, Palma-Silva C, Bered F, Cidade FW, Sousa ACB, Souza AP, Wendt T, Lexer C (2008) Isolation and characterization of microsatellite loci in Pitcairnia albiflos (Bromeliaceae), an endemic bromeliad from the Atlantic Rainforest, and cross–amplification in other species. Mol Ecol Resour 8:980–982

    Article  CAS  PubMed  Google Scholar 

  • Palma-Silva C, Cavallari MM, Barbará T, Lexer C, Gimenes MA, Bered F, Bodanese-Zanettini MH (2007) A set of polymorphic microsatellite loci for Vriesea gigantea and Alcantarea imperialis (Bromeliaceae) and cross amplification in other bromeliad species. Mol Ecol Notes 7:654–657

    Article  CAS  Google Scholar 

  • Palma-Silva C, Lexer C, Paggi GM, Barbará T, Bered F, Bodanese-Zanettini MH (2009) Range-wide patterns of nuclear and chloroplast DNA diversity in a Neotropical forest species, Vriesea gigantea (Bromeliaceae). Heredity 103:502–512

    Article  CAS  Google Scholar 

  • Palma-Silva C, Wendt T, Pinheiro F, Barbará T, Fay MF, Cozzolino S, Lexer C (2011) Sympatric bromeliad species (Pitcairnia spp.) facilitate tests of mechanisms involved in species cohesion and reproductive isolation in Neotropical inselbergs. Mol Ecol 20:3185–3201

    Article  CAS  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP: population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Article  Google Scholar 

  • Reitz R (1983) Bromeliáceas e a malária - bromélia endêmica. In: Reitz R Flora Ilustrada Catarinense (Fasc. Brom.) Herbário Barbosa Rodrigues, Itajaí

  • Santa-Rosa S, Souza FVD, Vidal AM, Ledo CAS, Santana JRF (2013) Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha. Hortic Bras 31(2):112–118

    Article  CAS  Google Scholar 

  • Sarthou C, Boisselier-Dubayle MC, Lambourdière J, Samadi S (2003) Polymorphic microsatellites for the study of fragmented populations of Pitcairnia geyskesii L. B. Smith (Bromeliaceae), a specific saxicolous species of inselbergs in French Guiana. Mol Ecol Notes 3:221–223

    Article  CAS  Google Scholar 

  • Scrok GJ, Varassin IG (2011) Reproductive biology and pollination of Aechmea distichantha Lem (Bromeliaceae). Acta Bot Bras 25(3):571–576

    Article  Google Scholar 

  • Smith LB (1934) Geographical evidence on the lines of evolution in Bromeliaceae. Bot Jahrb Syst 66:446–468

    Google Scholar 

  • Smith LB, Downs RJ (1979) Bromelioideae (Bromeliaceae). Flora Neotropica 14:1493–2141

    Google Scholar 

  • Turchetto-Zolet AC, Segatto ALA, Turchetto C, Palma-Silva C, Freitas LB (2013) Guia prático para estudos Filogeográficos. Ribeirão Preto

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  CAS  Google Scholar 

  • Versieux LM, Coffani-Nunes JV, Paggi GM, Costa AF (2018) Check-list of Bromeliaceae from Mato Grosso do Sul, Brazil. Iheringia Ser Bot 73:163–168

    Article  Google Scholar 

  • Vieira MLC, Santini L, Diniz AL, Munhoz CF (2016) Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol 39:312–328

    Article  PubMed  PubMed Central  Google Scholar 

  • Wörhmann T, Weising K (2011) In silico mining for simple sequence repeat loci in a pineapple expressed sequence tag database and cross-species amplification of EST-SSR markers across Bromeliaceae. Theor Appl Genet 123:635–647

    Article  Google Scholar 

  • Wörhmann T, Pinangé DSB, Krapp F, Benko-Iseppon AM, Huettel B, Weising K (2012) Development of 15 nuclear microsatellites markers in the genus Dyckia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing. Conserv Genet Resour 5:81–84

    Article  Google Scholar 

  • Zanella CM, Janke A, Paggi GM, Goetze M, Reis MS, Bered F (2012) Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads. Int J Mol Sci 13:15859–15866

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zenk FL, Firmer C, Wöhrmann T, Silva LV, Weising K, Huettel B, Paggi GM (2018) Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing. Appl Plant Sci 6:1–7

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 457698/2014-4), the Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT 23/200.245/2014) for fellowships and financial support, and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2018/07596-0).

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, 79070-900, Brazil

    Fernanda Maria de Russo Godoy & Gecele Matos Paggi

  2. Ciências Biológicas, Universidade Federal de Mato Grosso do Sul, Campus do Pantanal, Corumbá, MS, 79304-902, Brazil

    Gecele Matos Paggi

  3. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Barão Geraldo, Campinas, SP, 13083-862, Brazil

    Clarisse Palma-Silva

Authors
  1. Fernanda Maria de Russo Godoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gecele Matos Paggi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Clarisse Palma-Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

FMRG and GMP designed the study. FMRG performed the experiments and analyzed the data. FMRG, GMP and CP-S wrote the manuscript and reviewed the manuscript.

Corresponding author

Correspondence to Gecele Matos Paggi.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

de Russo Godoy, F.M., Matos Paggi, G. & Palma-Silva, C. Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae). Braz. J. Bot 42, 353–359 (2019). https://doi.org/10.1007/s40415-019-00528-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40415-019-00528-z

Keywords

Search

Navigation