Abstract
Cremanthodium Benth. is an endemic genus in the Himalayas and adjacent areas. Some plants of the genus are traditional medicinal plants in Tibetan medicine. In this study, the chloroplast genomes of five species (Cremanthodium arnicoides (DC. ex Royle) Good, Cremanthodium brunneopilosum S. W. Liu, Cremanthodium ellisii (Hook. f.) Kitam., Cremanthodium nervosum S. W. Liu, and Cremanthodium rhodocephalum Diels) were collected for sequencing. The sequencing results showed that the size of the chloroplast genome ranged from 150,985 to 151,284 bp and possessed a typical quadripartite structure containing one large single copy (LSC) region (83,326–83,369 bp), one small single copy (SSC) region (17,956–18,201 bp), and a pair of inverted repeats (IR) regions (24,830–24,855 bp) in C. arnicoides, C. brunneopilosum, C. ellisii, C. nervosum, and C. rhodocephalum. The chloroplast genomes encoded an equal number of genes, of which 88 were protein-coding genes, 37 were transfer ribonucleic acid genes, and eight were ribosomal ribonucleic acid genes, and were highly similar in overall size, genome structure, gene content, and order. In comparison with other species in the Asteraceae family, their chloroplast genomes share similarities but show some structural variations. There was no obvious expansion or contraction in the LSC, SSC or IR regions among the five species, indicating that the chloroplast gene structure of the genus was highly conserved. Collinearity analysis showed that there was no gene rearrangement. The results of the phylogenetic tree showed that the whole chloroplast genomes of the five species were closely related, and the plants of this genus were grouped into one large cluster with Ligularia Cass. and Farfugium Lindl.
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Data Availability
The data that support the findings of this study have been deposited in the NCBI database (GenBank accession: OM386855, OM386856, OM386857, OM386858, OM386859) (http://www.ncbi.nlm.nih.gov/).
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Acknowledgements
We thank Prof. Xiang Liu and Prof. Huarong Zhou for their assistance during leaves collection.
Funding
This work was supported by National Key Research and Development Program of China (No. 2019YFC1712300) and Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program (CXTD22002).
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WZ performed the experiments, data processing and manuscript draft preparation, XD contributed to analyzing the data, LC and ZM performed sample collection, XW and GZ designed the project and approved the final manuscript version.
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Zhong, W., Du, X., Wang, X. et al. Comparative analyses of five complete chloroplast genomes from the endemic genus Cremanthodium (Asteraceae) in Himalayan and adjacent areas. Physiol Mol Biol Plants 29, 409–420 (2023). https://doi.org/10.1007/s12298-023-01292-x
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DOI: https://doi.org/10.1007/s12298-023-01292-x