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Richness and biotechnological potential of the yeast community associated with the bromeliad phylloplane in the Brazilian Neotropical Forest

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Abstract

We studied the phylloplane-associated yeast communities of bromeliads at four sites in the Brazilian Northern Atlantic Forest through a culture-dependent approach and evaluated their potential as enzyme producers. A total of 213 isolates were identified by sequencing of D1/D2 region of the LSU rDNA gene or ITS region. Sequence analyses revealed that 182 isolates (85%) belong to the phylum Basidiomycota and 31 isolates (15%) to the phylum Ascomycota. The yeasts were identified as 86 species associated to 41 genera, reporting the highest phylloplane yeast richness in the literature to the present date. Besides, 59 strains were distributed in 32 possible undescribed species (38% of the total species in the study). Only two species occurred in all sampled sites: Carlosrosaea sp. nov. 3 and Papilliotrema flavescens, evidencing the heterogeneous character of yeast communities at these environments. At least one extracellular enzyme was detected in 173 isolates (85.6% of total yeast isolates), and one isolate of Aureobasidium thailandense was able to produce all five evaluated enzymes (amylase, cellulase, esterase, pectinase, and protease). The yeast community associated with bromeliads from the Atlantic Forest demonstrated a high heterogeneity and richness not yet found in previous studies on bromeliads, with a significant number of potential new species. Our results highlight bromeliads as a hotspot for yeast biodiversity studies.

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Data availability

The DNA sequences generated during and/or analyzed during the current study are available in the GenBank repository.

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Acknowledgments

The authors would like to thank the environmental entities responsible for issuing the authorizations to collect the material in the field, the Instituto Chico Mendes de Biodiversidade (ICMBio), the representatives of Parque Memorial Zumbi dos Palmares and Usina Caeté (Grupo Carlos Lyra) for the permissions, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, number of grants 475378/2013-0, 408718/2013-7, and 311553/2018-4), Fundação de Amparo a Pesquisa de Alagoas (FAPEAL), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). H.M.C.N. received a fellowship from PEC-PG/CNPq. C.R.F. and G.V.B.P. received a fellowship from CAPES. The authors also thank to Msc. Andressa Leticia Lopes for correcting and editing English.

Funding

This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, number of grants 475378/2013-0, 408718/2013-7, and 311553/2018-4), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de amparo a Pesquisa de Alagoas (FAPEAL).

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Author notes

  1. Hector Mauricio Casanova Navarro and Ciro R. Félix contributed equally to this work.

Authors and Affiliations

  1. Instituto de Ciências Biológicas e da Saúde, Setor de Genética (ICBS), Universidade Federal de Alagoas, Av. Lourival Melo Mota, s/n, Tabuleiro dos Martins, Maceio, AL, 57072-900, Brazil

    Hector Mauricio Casanova Navarro, Ciro R. Félix, Gustavo Vasconcelos B. Paulino, James Henrique Almeida & Melissa Fontes Landell

  2. Programa de Pós-graduação em Diversidade Biológica e Conservação nos Trópicos, Universidade Federal de Alagoas, Maceio, AL, Brazil

    Hector Mauricio Casanova Navarro, Ciro R. Félix & Gustavo Vasconcelos B. Paulino

  3. Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Patricia Valente

Authors
  1. Hector Mauricio Casanova Navarro
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  2. Ciro R. Félix
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  4. James Henrique Almeida
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  6. Melissa Fontes Landell
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Contributions

H.M.C.N.: formal analysis, investigation, data curation, writing—original draft; C.R.F.: formal analysis, investigation, data curation, writing - original draft, visualization; G.V.B.P.: formal analysis, writing—original draft; J.H.A.: investigation, data curation; P.V.: supervision, writing—review and editing; M.F.L.: conceptualization, supervision, project administration, funding acquisition, writing—review and editing.

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Correspondence to Melissa Fontes Landell.

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Casanova Navarro, H.M., Félix, C.R., Paulino, G.V.B. et al. Richness and biotechnological potential of the yeast community associated with the bromeliad phylloplane in the Brazilian Neotropical Forest. Mycol Progress 19, 1387–1401 (2020). https://doi.org/10.1007/s11557-020-01631-2

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