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In vitro tuberization and plant regeneration from multinodal segment culture of Habenaria bractescens Lindl., an Argentinean wetland orchid

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Abstract

Tuberization in many terrestrial orchids represents the most important physiological process for reproduction and survival. An in vitro controlled and reproducible tuberization and plant regeneration system was designed for Habenaria bractescens. Multinodal segments were incubated on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzylaminopurine (BAP) and sucrose. After 45 days, the explants developed root tubers in vitro in 8 of the 12 media assayed. MS medium with 87.6 mM sucrose plus 4.4 μM BAP was one of the most effective for stimulating root tubers. Plants derived from in vitro root tubers were successfully transferred to the greenhouse without any acclimatization. The morphology and anatomy of the regenerated underground organs were examined to find identifying and distinguishing features. The protocol to regenerate root tubers of H. bractescens will be useful to study the basic aspects and control of tuberization and to carry out restoration programs.

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Acknowledgments

The authors are grateful to the FPLA’S (Fondo Para Las Américas), FORA (Federación Civil de Orquideófilos de la República Argentina), CONICET and SGCyT (Secretaría General de Ciencia y Técnica, UNNE) for the financial support. The authors wish to express their gratitude to Beth Hazen, Ph.D. and Ernestina Galdeano, Ph.D., for their valuable comments on the manuscript.

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  1. Instituto de Botánica del Nordeste (CONICET − Consejo Nacional de Investigaciones Científicas y Técnicas), Facultad de Ciencias Agrarias (UNNE − Universidad Nacional del Nordeste), Casilla de Correo 209, 3400, Corrientes, Argentina

    Ricardo Daniel Medina, Eduardo Alberto Flachsland, Ana María Gonzalez, Graciela Terada, Mirta Mabel Faloci & Luis Amado Mroginski

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  1. Ricardo Daniel Medina
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  2. Eduardo Alberto Flachsland
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Correspondence to Ricardo Daniel Medina.

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Medina, R.D., Flachsland, E.A., Gonzalez, A.M. et al. In vitro tuberization and plant regeneration from multinodal segment culture of Habenaria bractescens Lindl., an Argentinean wetland orchid. Plant Cell Tiss Organ Cult 97, 91–101 (2009). https://doi.org/10.1007/s11240-009-9505-2

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