Abstract
Clematis chlorotic mottle virus (ClCMV) is a previously undescribed virus associated with symptoms of yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and flower distortion and discoloration on ornamental Clematis. The ClCMV genome is 3,880 nt in length with five open reading frames (ORFs) encoding a 27-kDa protein (ORF 1), an 87-kDa replicase protein (ORF 2), two centrally located movement proteins (ORF 3 and 4), and a 37-kDa capsid protein (ORF 5). Based on morphological, genomic, and phylogenetic analysis, ClCMV is predicted to be a member of the genus Pelarspovirus in the family Tombusviridae.
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All authors declare they have no conflict of interest. The research reported in this manuscript did not involve experimentation with human or animal subjects. Plant samples were moved within the U.S.A. under USDA APHIS permit P526P-13-03719 and from the U.K. under permit P526P-14-00688.
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705_2017_3236_MOESM2_ESM.tif
Supplementary Figure S1 An internal replication element (IRE) indentified by Mfold RNA secondary structure prediction analysis within the RdRp of the genome of ClCMV is similar to those found in other members of the family Tombusviridae, including viruses of the genus Pelarspovirus. This IRE contains one distinct conserved motif (5’-CAU UCC-3’/5’-GGC AGG-3’) within the Tombusvirdae IRE motif 2, which has been identified in other members of the genus Pelarspovirus and is in ClCMV at nt 1020 and nt 1069. The nucleotides in the internal loop motifs that are conserved within the genus Pelarspovirus are shown in red, and nucleotides corresponding to the CC mismatch in tombusviruses are shown in green. (TIFF 30 kb)
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McLaughlin, M., Lockhart, B., Jordan, R. et al. Complete nucleotide sequence of clematis chlorotic mottle virus, a new member of the family Tombusviridae . Arch Virol 162, 1373–1379 (2017). https://doi.org/10.1007/s00705-017-3236-1
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DOI: https://doi.org/10.1007/s00705-017-3236-1