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Taxonomic revision of Delphinium (Ranunculaceae) in the South-East of European Russia

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Abstract

Morphological and phylogenetic (nrITS barcode) analyses are conducted to clarify the taxonomic status of 10 Delphinium species (D. cuneatum, D. dictyocarpum, D. duhmbergii, D. elatum, D. litwinowii, D. pubiflorum, D. puniceum, D. sergii, D. subcuneatum, D. uralense) occurring in the South-East of European Russia. The morphometric analysis is carried out with 22 quantitative and 32 qualitative parameters. Based on all parameters, the nMDS supports the differentiation of D. puniceum, D. sergii, D. uralense and D. pubiflorum whereas other studied taxa remain undistinguished. Furthermore, the Random forest analysis and the MrBayes phylogenetic analysis of ITS sequences confirm the species status of D. puniceum belonging to D. sect. Diedropetala and D. elatum, D. uralense, D. dictyocarpum and D. pubiflorum belonging to D. sect. Delphinastrum. Finally, recursive partitioning is performed to develop the dichotomous key which can be used to identify the species of Delphinium in the study area. In conclusion, we stress that the recognition of species belonging to D. sect. Delphinastrum is hindered by the presence of numerous intermediate or hybrid forms, on the one hand, and the impact of climatic conditions on the morphological (and, specifically, taxonomically significant) traits, on the other hand.

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Acknowledgements

The reported study was funded by Russian Fund for Basic Research (RFBR), Project Number 18-34-00061.

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Authors and Affiliations

  1. Botanic Garden, Saratov State University, Astrakhanskaya Str., 83, Saratov, Russian Federation, 410012

    Alexander S. Kashin, Tatyana A. Kritskaya, Artem V. Bogoslov, Irina V. Shilova & Alena S. Parkhomenko

  2. Department of Biology, Bashkir State University, Zaki Validi Str., 32, Ufa, Russian Federation, 450076

    Maya M. Ishmuratova

  3. Bashkir State Natural Sanctuary, Karat Str., 2, Starosubkhangulovo, Bashkortostan Republic, Russian Federation, 453580

    Maya M. Ishmuratova

  4. Ufa Institute of Biology – Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences, October Prospect, 71, Ufa, Russian Federation, 450054

    Nikolai I. Fedorov

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  1. Alexander S. Kashin
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Correspondence to Tatyana A. Kritskaya.

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Supplementary Information

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Information on Electronic Supplementary Material

Online Resource 1. Morphological parameters and their variations used in the morphometric analysis of Delphinium taxa.

Online Resource 2. Morphological parameters of leaf blade (following Anatov et al. 2015).

Online Resource 3. Morphological parameters of flower, bracts and bracteoles.

Online Resource 4. GenBank accession numbers of studied populations of Delphinium species in the south-east of European Russia.

Online Resource 5. Command history and results of statistical processing of the morphometric data in the R environment.

Online Resource 6. Haplotype network of ITS sequences of 9 Delphinium taxa built in the TCS software. The specimen abbreviations are the same as in Figure 1 and Table 2.

Online Resource 7. Maximum Likelihood tree built with ITS sequences of 9 Delphinium taxa using the Tamura-Nei model.

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Kashin, A.S., Kritskaya, T.A., Bogoslov, A.V. et al. Taxonomic revision of Delphinium (Ranunculaceae) in the South-East of European Russia. Plant Syst Evol 307, 59 (2021). https://doi.org/10.1007/s00606-021-01783-y

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