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Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping

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Abstract

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Association and linkage mapping techniques were used to identify and verify single nucleotide polymorphisms (SNPs) associated with Sclerotinia sclerotiorum resistance. A novel resistant gene, GmGST , was cloned and shown to be involved in soybean resistance to SSR.

Abstract

Sclerotinia stem rot (SSR), caused by the fungus Sclerotinia sclerotiorum, is one of the most devastating diseases in soybean (Glycine max (Linn.) Merr.) However, the genetic architecture underlying soybean resistance to SSR is poorly understood, despite several mapping and gene mining studies. In the present study, the identification of quantitative trait loci (QTLs) involved in the resistance to S. sclerotiorum was conducted in two segregating populations: an association population that consisted of 261 diverse soybean germplasms, and the MH population, derived from a cross between a partially resistant cultivar (Maple arrow) and a susceptible cultivar (Hefeng25). Three and five genomic regions affecting resistance were detected by genome-wide association study to control the lesion length of stems (LLS) and the death rate of seedling (DRS), respectively. Four QTLs were detected to underlie LLS, and one QTL controlled DRS after SSR infection. A major locus on chromosome (Chr.) 13 (qDRS13-1), which affected both DRS and LLS, was detected in both the natural population and the MH population. GmGST, encoding a glutathione S-transferase, was cloned as a candidate gene in qDRS13-1. GmGST was upregulated by the induction of the partially resistant cultivar Maple arrow. Transgenic experiments showed that the overexpression of GmGST in soybean increased resistance to S. sclerotiorum and the content of soluble pigment in stems of soybean. The results increase our understanding of the genetic architecture of soybean resistance to SSR and provide a framework for the future marker-assisted breeding of resistant soybean cultivars.

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Acknowledgements

This study was conducted in the Key Laboratory of Soybean Biology of the Chinese Education Ministry, Soybean Research & Development Center (CARS), and the Key Laboratory of Northeastern Soybean Biology and Breeding/Genetics of the Chinese Agriculture Ministry and was financially supported by the Chinese National Natural Science Foundation (31871650, 31671717, 31471517, 31971967), National Key R & D Project (2016YFD0100304, 2017YFD0101306, 2017YFD0101302), Heilongjiang Provincial Project (JC2018007, GX17B002, C2018016, GJ2018GJ0098),the National Project (2014BAD22B01, 2016ZX08004001-007), the Youth Leading Talent Project of the Ministry of Science and Technology in China (2015RA228), The National Ten-thousand Talents Program, Postdoctoral Fund in Heilongjiang Province (LBH-Z15017), The national project (CARS-04-PS04). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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  1. Zou Jianan, Wenjing Li and Yuting Zhang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Soybean Biology in Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin, 150030, China

    Zou Jianan, Wenjing Li, Yuting Zhang, Wei Song, Haipeng Jiang, Yuhang Zhan, Weili Teng, Xue Zhao & Yingpeng Han

  2. Zhumadian Academy of Agricultural Sciences, Zhumadian, 463000, China

    Jingyun Zhao

  3. Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Lijuan Qiu

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  1. Zou Jianan
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Contributions

YPH and XZ designed and supervised the research; JNZ, WJL and YTZ conducted the experiment and analyzed the data; WS, HPJ, YHZ, JYZ and WLT conducted the phenotype identification and transgenic work. XZ, JNZ and LJQ wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Xue Zhao or Yingpeng Han.

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Jianan, Z., Li, W., Zhang, Y. et al. Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping. Theor Appl Genet 134, 2699–2709 (2021). https://doi.org/10.1007/s00122-021-03855-6

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