Fig 1.
A-G Direct multiple shoot organogenesis from in vitro root explants of Citrus jambhiri Lush. [A: Seedling; B, C, D: Nodulation in root explants, multiple shoot organogenesis, multiple shoot elongation at MSN+BA 1.0+GA3 1.0 mg L-1, respectively; E, F, G: Nodulation in root explants, multiple shoot organogenesis, multiple shoot elongation at MSN+BA 1.0+GA3 2.0 mg L-1, respectively]. DOI 10.17605/OSF.IO/CFT75.
Table 1.
Effect of Nitsch vitamin, cytokinins and gibberellic acid (GA3) on direct multiple shoots organogenesis from in vitro root explants of Citrus jambhiri Lush.
Fig 2.
A-J Root induction in in vitro plantlets of Citrus jambhiri Lush. [A: ½MSN; B-C: ½MSN+NAA 1.0 and 2.0 mg L-1; D-E: ½MSN+IAA 1.0 and 2.0 mg L-1; F: MSN; G-H: MSN+NAA 1.0 and 2.0 mg L-1; I-J: MSN+IAA 1.0 and 2.0 mg L-1]. DOI 10.17605/OSF.IO/FR7X5.
Table 2.
Effect of Nitsch vitamin and auxins on root induction in the shootlets derived from in vitro root explants of Citrus jambhiri Lush.
Fig 3.
A-D Relative expression of growth regulating factors (A. GRF1 and B. GRF5) and GA3 pathway genes (C. GA2OX1 and D. KO1) at different stages of organogenesis from in vitro root explants of Citrus jambhiri Lush. DOI 10.17605/OSF.IO/XGUFT.
Fig 4.
A-F Relative expression of auxin responsive factors (A. ARF1 and B. ARF8), auxin efflux carrier protein genes (C. PIN1 and D. PIN5), auxin/IAA family protein gene (E. IAA4) and GH3 family protein gene (F. GH3) at different stages of rooting of Citrus jambhiri Lush. DOI 10.17605/OSF.IO/C27QB.
Fig 5.
A-C Scanning electron micrograph of the roots induced in Citrus jambhiri Lush. plantlets using various concentrations of auxins [A. ½MSN (A1-A2: External at 500 and 1000x; A3-A4: Internal at 1500 and 2000x), B. ½MSN+NAA 1.0 mg L-1(B1-B2: External at 500 and 1000x; B3-B4: Internal at 1500 and 2000x), C. ½MSN+IAA 1.0 mg L-1 (C1-C2: External at 500 and 1000x; C3-C4: Internal at 1500 and 2000x)]. DOI 10.17605/OSF.IO/GP6AH.
Fig 6.
A-B Indexing of the regenerants employing Las F and R (500 bp) to detect Citrus greening (Huanglongbing) [A] and K607 F and K608 R (648 bp) to detect Citrus tristeza retro virus [B] in Citrus jambhiri Lush. regenerants [Lane M: 100 bp ladder (GCC biotech); Lane 1: Positive control (C. greening or CTV infected leaf midrib); Lane 2: leaf midrib from the mother plant; Lane 3: In vitro leaf midrib from the plantlets (½MSN); Lane 4: In vitro leaf midrib from the plantlets (½MSN+NAA 1.0 mg L-1); Lane 5: In vitro leaf midrib from the plantlets (½MSN+IAA 1.0 mg L-1); Lane 6: primer control (negative control)]. DOI 10.17605/OSF.IO/YDNKU.
Fig 7.
A-B Genetic fidelity of Citrus jambhiri Lush. regenerants employing RAPD (A. OPU 05) and ISSR (B. UBC 810) markers [Lane M: 1 kb DNA ladder for RAPD and ISSR; Lane 1: Mother plant from Kachai village, Ukhrul, Manipur; Lane 2: In vitro seedlings; Lane 3: Seedlings planted at Langol farm of ICAR, Manipur; Lane 4: Seedlings planted at polyhouse of ICAR, Manipur; Lane 5: Regenerants obtained from MSN+BAP 1.0+GA3 1.0 mg L-1; Lane 6: Regenerants obtained from MSN+BAP 1.0+GA3 2.0 mg L-1; Lane 7: Plantlets obtained from ½MSN; Lane 8: Plantlets obtained from ½MSN+NAA 1.0 mg L-1; Lane 9: Plantlets obtained from ½MSN+IAA 1.0 mg L-1]. DOI 10.17605/OSF.IO/9YC6V.
Table 3.
Polymerase chain reaction amplicons obtained from RAPD and ISSR analysis of in vitro regenerants of C. jambhiri Lush.
Fig 8.
A-G Survivability of in vitro plantlets from different concentrations of auxins [A. ½MSN, B. ½MSN+NAA (1.5 mg L-1), C. ½MSN+IAA (1.0 mg L-1), D. MSN, E. MSN+NAA (1.5 mg L-1), F. MSN+IAA (1.0 mg L-1), G. %Survivability of the in vitro plantlets derived from MSN+BA 1.0+GA3 1.0 mg L-1 and different concentrations of auxins] DOI 10.17605/OSF.IO/R67JZ.